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https://read.qxmd.com/read/30771677/phenol-removal-by-laccases-and-other-phenol-oxidases-of-pleurotus-sajor-caju-ps-2001-in-submerged-cultivations-and-aqueous-mixtures
#1
Fernanda Bettin, Francine Cousseau, Kamila Martins, Nicole Amanda Boff, Simone Zaccaria, Mauricio Moura da Silveira, Aldo José Pinheiro Dillon
In this work, phenol removal from aqueous solutions by Pleurotus sajor-caju PS-2001 phenol oxidases was assessed under different conditions. In stirred-tank reactor (STR), 77, 82, 92 and 36% of removal were attained when initial concentrations of 1.0, 2.0, 3.0 and 4.0 mmol L-1 phenol, respectively, were used. Among the different enzymes produced by this fungus, phenol removal seems to be related to the activity of laccases that attained maximum values between 33 and 91 U mL-1 in STR. With an internal-loop airlift reactor (ILAR), phenol concentrations of 1...
February 13, 2019: Journal of Environmental Management
https://read.qxmd.com/read/30771293/macro-and-microfilaricidal-activities-of-extracts-of-annona-senegalensis-and-milletia-comosa-against-onchocerca-ochengi-and-loa-loa
#2
Adela Ngwewondo, Faustin Pascal Tsague Manfo, Moses Samje, Elvis Monya, Fidelis Cho-Ngwa
Despite the efforts employed for the control of onchocerciasis, the latter has remained a significant public health problem, due mainly to the lack of safe and effective adult worm drugs and/or microfilaricides that do not kill Loa loa microfilariae (mf). Serious adverse events have been encountered after administering ivermectin to some onchocerciasis patients coinfected with Loa loa. There is therefore, an urgent need for a macro and/or microfilaricidal drug which kills Onchocerca but not L. loa microfilariae...
February 13, 2019: Experimental Parasitology
https://read.qxmd.com/read/30770357/prevalence-and-risk-factors-of-myopic-maculopathy-in-rural-southern-china-the-yangxi-eye-study
#3
Zhixi Li, Ran Liu, Guangming Jin, Jason Ha, Xiaohu Ding, Wei Xiao, Xiao Xu, Lei An, Jialiang Zhao, Mingguang He
AIMS: To evaluate the prevalence of myopic maculopathy among participants aged 50 years and older in rural southern China. METHODS: Population-based, cross-sectional study. Subjects were recruited using cluster sampling from Yangxi County, Guangdong Province, China from August to November in 2014. Non-cycloplegic autorefraction and fundus photography were performed on all of the participants. Myopic maculopathy was graded based on fundus photographs using the International Classification of Myopic Maculopathy...
February 15, 2019: British Journal of Ophthalmology
https://read.qxmd.com/read/30770138/identification-of-polcalcin-as-a-novel-allergen-of-amaranthus-retroflexus-pollen
#4
M Vakili Moghaddam, M Fallahpour, M Mohammadi, F S Rasi Varaee, K Mokhtarian, M Khoshmirsafa, R Jafari, N Shirzad, R Falak
INTRODUCTION: Amaranthus retroflexus (Redroot Pigweed) is one of the main sources of allergenic pollens in temperate areas. Polcalcin is a well-known panallergen involved in cross-reactivity between different plants. The aim of this study was the molecular cloning and expression of polcalcin, as well as evaluating its IgE-reactivity with A. retroflexus sensitive patients' sera. METHODS: Allergenic extract was prepared from A. retroflexus pollen and the IgE-reactivity profile was determined by ELISA and immunoblotting using sera from twenty A...
February 12, 2019: Allergologia et Immunopathologia
https://read.qxmd.com/read/30769892/milk-yield-milk-composition-and-the-nutritive-value-of-feed-accessed-varies-with-milking-order-for-pasture-based-dairy-cattle
#5
Kamila Dias, Sergio Garcia, Mohammed Rafiq Islam, Cameron Clark
(1) Background: Pasture varies in its chemical composition from the top of the sward to the base and cattle prefer to eat the leaf fraction. In pasture-based dairy systems, cattle predominantly walk back to pasture voluntarily after each milking, with the first cattle arriving to pasture hours before the last. Here we study the impact of pasture composition according to milking order on milk yield and milk composition for dairy cattle offered grazed ryegrass pasture. (2) Methods: In the first experiment, individual cow milk yield data were recorded on six farms over 8 months...
February 14, 2019: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/30769784/biocatalysis-of-fucodian-in-undaria-pinnatifida-sporophyll-using-bifidobacterium-longum-rd47-for-production-of-prebiotic-fucosylated-oligosaccharide
#6
Jeong Min Lee, So Young Oh, Tony V Johnston, Seockmo Ku, Geun Eog Ji
Fucosylated oligosaccharide (FO) is known to selectively promote the growth of probiotic bacteria and is currently marketed as a functional health food and prebiotic in infant formula. Despite widespread interest in FO among functional food customers, high production costs due to high raw material costs, especially those related to fucose, are a significant production issue. Therefore, several actions are required before efficient large-scale operations can occur, including (i) identification of inexpensive raw materials from which fucosylated oligosaccharides may be produced and (ii) development of production methods to which functional food consumers will not object (e...
February 14, 2019: Marine Drugs
https://read.qxmd.com/read/30769063/a-randomized-controlled-trial-comparing-crude-versus-heparin-bonded-prfe-graft-in-below-the-knee-bypass-surgery-for-critical-limb-ischemia-replace-trial-design-and-protocol
#7
Yann Gouëffic, Jean-Pierre Favre, Eric Steinmetz, Aline Ordureau, Valéry-Pierre Riche, Béatrice Guyomarch, Eugenio Rosset
BACKGROUND: While autogenous venous graft is preferable in below the knee bypass surgery for patients in critical limb ischemia, some 20% of patients will have no suitable vein for grafting, compelling a resort to artificial graft materials. Retrospective sub-group analyses within published studies comparing heparin-bonded PTFE with crude PFTE graft in below the knee bypass suggest that heparin-bonded graft offers superior long-term patency rates, but this has not been prospectively verified in patients with critical limb ischemia...
February 12, 2019: Annals of Vascular Surgery
https://read.qxmd.com/read/30769041/stem-extract-of-tabebuia-chrysantha-induces-apoptosis-by-targeting-segfr-in-ehrlich-ascites-carcinoma
#8
Siva Prasad Panda, Uttam Prasad Panigrahy, Subhranshu Panda, Bikash R Jena
ETHNOPHARMACOLOGICAL RELEVANCE: The plant Tabebuia chrysantha (Jaq.) Nicholson (Bignoniaceae) is commonly known as "Golden Goddess" in the Southern part of India and "Golden Trumpet Tree " in Central America. Stems of this plant have been traditionally used for antioxidant, anti-inflammatory, antimicrobial and anticancer actions. AIM OF THE STUDY: To evaluate the antitumor activity of methanol extract of Tabebuia chrysantha stem (METC). MATERIALS AND METHODS: The in vivo antitumor potential of METC against Ehrlich Ascites Carcinoma (EAC) in Swiss albino mice was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight, hematological parameters, biochemical parameters, and antioxidant parameters...
February 12, 2019: Journal of Ethnopharmacology
https://read.qxmd.com/read/30768183/household-saving-during-pregnancy-and-facility-delivery-in-zambia-a-cross-sectional-study
#9
Calvin Chiu, Nancy A Scott, Jeanette L Kaiser, Thandiwe Ngoma, Jody R Lori, Carol J Boyd, Peter C Rockers
Financial barriers cause many women in low- and middle-income countries to deliver outside of a health facility, contributing to maternal and neonatal mortality. Savings accrued during pregnancy can increase access to safe delivery services. We investigated the relationship between household saving during pregnancy and facility delivery. A cross-section of 2381 women who delivered a child in the previous 12 months was sampled from 40 health facility catchment areas across eight districts in three provinces in Zambia in April and May of 2016...
February 14, 2019: Health Policy and Planning
https://read.qxmd.com/read/30767927/isolation-screening-and-crude-oil-degradation-characteristics-of-hydrocarbons-degrading-bacteria-for-treatment-of-oily-wastewater
#10
Xiumei Tian, Xiaoli Wang, Shitao Peng, Zhi Wang, Ran Zhou, He Tian
The aim of this study was to isolate hydrocarbons-degrading bacteria for treatment of oily wastewater from long-standing petroleum-polluted sediments in Bohai Bay, China. Six hydrocarbons-degrading bacteria were screened and identified as Pseudomonas sp. and Bacillus sp. A new approach using a combination of various bacterial species in petroleum biodegradation was proposed and evaluated for its degradation characteristics. Gas chromatography-flame ionization detection (GC-FID) analysis showed that mixed bacterial agents (YJ01) degraded 80...
December 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://read.qxmd.com/read/30767622/characterization-of-medicinal-plant-associated-biocontrol-bacillus-subtilis-ssl2-by-liquid-chromatography-mass-spectrometry-and-evaluation-of-compounds-by-in-silico-and-in-vitro-methods
#11
Hardik Jinal Naik, Natarajan Amaresan
This study explores the antimicrobial properties of bioactive secondary metabolites extracted from the medicinal plant (Solanum surattense) associated Bacillus subtilis strain SSL2. The secondary metabolites were extracted from B. subtilis (SSL2) using ethyle acetate, acetone, butanol, chloroform, and methanol solvents. The crude extract was tested against two wilt causing pathogens: Ralstonia solanacearum and Fusarium oxysporum. The results revealed that the ethyl acetate extract has maximum inhibition against both the pathogens tested in the study...
February 15, 2019: Journal of Biomolecular Structure & Dynamics
https://read.qxmd.com/read/30766624/the-evolution-of-the-anterior-capsulotomy
#12
REVIEW
Dorota Wyględowska-Promieńska, Marcin Jaworski, Kamila Kozieł, Richard Packard
The paper describes the development of the anterior capsulotomy from its early crude beginnings in the 18th century to the possibility of automated surgery today via continuous curvilinear capsulorhexis (CCC). The reasons for the opening of the capsule have changed from a roughly made tear to allow access to the nucleus for its extraction, to the creation of more regular openings to allow support for intraocular lenses. With the development of continuous circular tears it was possible to be certain to contain the intraocular lens (IOL) in the capsular bag...
January 2019: Wideochirurgia i Inne Techniki Mało Inwazyjne, Videosurgery and Other Miniinvasive Techniques
https://read.qxmd.com/read/30766603/isolation-of-polygalacturonase-producing-bacterial-strain-from-tomatoes-lycopersicon-esculentum-mill
#13
Yemisi Dorcas Obafemi, Adesola Adetutu Ajayi, Olugbenga Samson Taiwo, Shade John Olorunsola, Patrick Omoregie Isibor
Background: Polygalacturonase (EC 3.2.1.15) enzyme aids in microbial spoilage of fruits and vegetables. It is very important to find economical ways to producing the enzyme so as to achieve maximum yield in industries due to its use at different areas of production process. Methods: Isolation of polygalacturonase-producing bacterial strain from tomatoes ( Lycopersicon esculentum Mill.) was studied. Polygalacturonase-producing bacterial strains were isolated and screened from tomatoes stored at normal laboratory temperature (25 ± 2°C)...
2019: International Journal of Microbiology
https://read.qxmd.com/read/30766543/polysaccharides-derived-from-the-brown-algae-lessonia-nigrescens-enhance-salt-stress-tolerance-to-wheat-seedlings-by-enhancing-the-antioxidant-system-and-modulating-intracellular-ion-concentration
#14
Ping Zou, Xueli Lu, Hongtao Zhao, Yuan Yuan, Lei Meng, Chengsheng Zhang, Yiqiang Li
Soil salinity reduces plant growth and is a major factor that causes decreased agricultural productivity worldwide. Seaweed polysaccharides promote crop growth and improve plant resistance to abiotic stress. In this study, polysaccharides from brown seaweed Lessonia nigrescens polysaccharides (LNP) were extracted and further separated and fractionated. Two acidic polysaccharides (LNP-1 and LNP-2) from crude LNP were obtained and characterized. The latter had a lower molecular weight (MW) (40.2 kDa) than the former (63...
2019: Frontiers in Plant Science
https://read.qxmd.com/read/30766425/comparative-evaluation-of-cytotoxic-antimicrobial-and-antioxidant-activities-of-the-crude-extracts-of-three-plectranthus-species-grown-in-saudi-arabia
#15
Ramzi A Mothana, Jamal M Khaled, Ali A El-Gamal, Omar M Noman, Ashok Kumar, Mohamed F Alajmi, Adnan J Al-Rehaily, Mansour S Al-Said
Natural products from medicinal plants represent major resource of novel therapeutic substances for combating serious diseases including cancers and microbial infections. The genus Plectranthus (Family: Labiatae) represents a large and widespread group of species with a diversity of traditional uses in treatment of various ailments. Therefore, this research study aimed to evaluate the cytotoxic, antimicrobial and antioxidant activities of three Plectranthus species growing in Saudi Arabia namely P. cylindraceus Hocst...
February 2019: Saudi Pharmaceutical Journal: SPJ: the Official Publication of the Saudi Pharmaceutical Society
https://read.qxmd.com/read/30764792/association-of-vitamin-d-receptor-gene-variants-with-polycystic-ovary-syndrome-a-meta-analysis
#16
Xiao-Yuan Shi, Ai-Ping Huang, Duo-Wen Xie, Xiao-Long Yu
BACKGROUND: Polycystic ovary syndrome (PCOS) is a common endocrine disorder in reproductive-age women. Multiple susceptible gene as well as environmental factors and their interaction each other are contributed to the PCOS risk. Several case-control studies have researched the associations of the vitamin D receptor gene (VDR) polymorphisms with PCOS susceptibility, but the jury is still out. Here, we carried out a meta-analysis to clarify polymorphisms between ApaI (C/A) (rs7975232), BsmI (G/A) (rs1544410), FokI (C/T) (rs10735810), TaqI (T/C) (rs731236) and Tru9I (G/A) (rs757343) in the VDR gene and PCOS susceptibility based on relative lager sample size...
February 14, 2019: BMC Medical Genetics
https://read.qxmd.com/read/30763949/maternal-hypertension-and-survival-in-singletons-and-twins-born-at-23-29-weeks-not-just-one-answer%C3%A2
#17
Luigi Gagliardi, Olga Basso
OBJECTIVE: To describe the association between maternal hypertension (chronic and gestational, MH) and mortality in very preterm singletons and twins, focusing on how estimates depend on gestational age (GA) and size at birth. METHODS: We estimated relative risks of in-hospital death in 12,320 singletons (MH: 22.4%) and 4381 twins (MH: 10.6%) born at 23-29 weeks in the Italian Neonatal Network (89 hospitals, 2008-2016). RESULTS: Babies with MH had higher GA and were more frequently small-for-gestational age (SGA), especially singletons...
February 14, 2019: Pediatric Research
https://read.qxmd.com/read/30763864/biochemical-response-of-indigenous-microalgal-consortia-to-variations-in-nitrogen-concentration-of-treated-effluent
#18
Hironori Okazaki, Yugo Takabe, Takanori Masuda, Yoshiko Hoshikawa
Cultivation conditions influence microalgal cellular components, such as lipid accumulation under nutrient depletion, high light irradiation and salinity stress. In this study, indigenous microalgal consortia were cultivated in batch mode using an actual treated effluent. The temporal response of cellular components to the variations in nitrogen concentration and influence of light irradiation on the response were investigated. Prolonged exposure of indigenous microalgal consortia to nitrogen exhaustion had minor effects on total lipid accumulation and enhancement of energy content...
February 4, 2019: Bioresource Technology
https://read.qxmd.com/read/30763379/in-vitro-evaluation-of-the-protective-effects-of-plant-extracts-against-amyloid-beta-peptide-induced-toxicity-in-human-neuroblastoma-sh-sy5y-cells
#19
Ana Luiza Sereia, Marcelo Tempesta de Oliveira, Adrivanio Baranoski, Leila Larisa Medeiros Marques, Fabianne Martins Ribeiro, Raquel Garcia Isolani, Daniela Cristina de Medeiros, Danielly Chierrito, Danielle Lazarin-Bidóia, Acácio Antonio Ferreira Zielinski, Cláudio Roberto Novello, Celso Vataru Nakamura, Mário Sérgio Mantovani, João Carlos Palazzo de Mello
Alzheimer's disease (AD) is the most common form of dementia and has no cure. Therapeutic strategies focusing on the reduction of oxidative stress, modulation of amyloid-beta (Aβ) toxicity and inhibition of tau protein hyperphosphorylation are warranted to avoid the development and progression of AD. The aim of this study was to screen the crude extracts (CEs) and ethyl-acetate fractions (EAFs) of Guazuma ulmifolia, Limonium brasiliense, Paullinia cupana, Poincianella pluviosa, Stryphnodendron adstringens and Trichilia catigua using preliminary in vitro bioassays (acetylcholinesterase inhibition, antioxidant activity and total polyphenol content) to select extracts/fractions and assess their protective effects against Aβ25-35 toxicity in SH-SY5Y cells...
2019: PloS One
https://read.qxmd.com/read/30763314/assessing-effects-of-germline-exposure-to-environmental-toxicants-by-high-throughput-screening-in-c-elegans
#20
Nara Shin, Luciann Cuenca, Rajendiran Karthikraj, Kurunthachalam Kannan, Monica P Colaiácovo
Chemicals that are highly prevalent in our environment, such as phthalates and pesticides, have been linked to problems associated with reproductive health. However, rapid assessment of their impact on reproductive health and understanding how they cause such deleterious effects, remain challenging due to their fast-growing numbers and the limitations of various current toxicity assessment model systems. Here, we performed a high-throughput screen in C. elegans to identify chemicals inducing aneuploidy as a result of impaired germline function...
February 2019: PLoS Genetics
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