keyword
https://read.qxmd.com/read/37823055/monitoring-of-single-extracellular-vesicle-heterogeneity-in-cancer-progression-and-therapy
#21
REVIEW
Yoon-Jin Lee, Shinwon Chae, Dongsic Choi
Cancer cells actively release lipid bilayer extracellular vesicles (EVs) that affect their microenvironment, favoring their progression and response to extracellular stress. These EVs contain dynamically regulating molecular cargos (proteins and nucleic acids) selected from their parental cells, representing the active biological functionality for cancer progression. These EVs are heterogeneous according to their size and molecular composition and are usually defined based on their biogenetic mechanisms, such as exosomes and ectosomes...
2023: Frontiers in Oncology
https://read.qxmd.com/read/37785000/the-effect-of-mdsc-derived-exosomes-played-in-esophageal-squamous-carcinoma-cells-after-ionizing-radiation
#22
JOURNAL ARTICLE
R Sun, K Xi, X Song, W Yin, D Xi, Y Shao, W Gu, J Jiang
PURPOSE/OBJECTIVE(S): Radiotherapy is the main treatment for esophageal cancer. Previous studies have shown that radiotherapy not only kills tumor cells directly, but also reshapes the immune microenvironment of the tumor. It has been reported an increase in the recruitment of myeloid-derived suppressor cells (MDSC) can occur in tumor tissue after ionizing radiation. Exosomes are mediators of intercellular information exchange and are also involved in the regulation of the tumor microenvironment...
October 1, 2023: International Journal of Radiation Oncology, Biology, Physics
https://read.qxmd.com/read/37695590/engineered-exosomes-as-theranostic-platforms-for-cancer-treatment
#23
REVIEW
Xu Yang, Xiangyu Xie, Songbin Liu, Wei Ma, Zhi Zheng, Hua Wei, Cui-Yun Yu
Tremendous progress in nanotechnology and nanomedicine has made a significant positive effect on cancer treatment by integrating multicomponents into a single multifunctional nanosized delivery system for combinatorial therapies. Although numerous nanocarriers developed so far have achieved excellent therapeutic performance in mouse models via elegant integration of chemotherapy, photothermal therapy, photodynamic therapy, sonodynamic therapy, and immunotherapy, their synthetic origin may still cause systemic toxicity, immunogenicity, and preferential detection or elimination by the immune system...
September 11, 2023: ACS Biomaterials Science & Engineering
https://read.qxmd.com/read/37644215/multiomics-and-blood-based-biomarkers-of-electroconvulsive-therapy-in-severe-and-treatment-resistant-depression-study-protocol-of-the-detect-study
#24
JOURNAL ARTICLE
Iven-Alex von Mücke-Heim, Julius C Pape, Norma C Grandi, Angelika Erhardt, Jan M Deussing, Elisabeth B Binder
Electroconvulsive therapy (ECT) is commonly used to treat treatment-resistant depression (TRD). However, our knowledge of the ECT-induced molecular mechanisms causing clinical improvement is limited. To address this issue, we developed the single-center, prospective observational DetECT study ("Multimodal Biomarkers of ECT in TRD"; registered 18/07/2022, www.clinicalTrials.gov , NCT05463562). Its objective is to identify molecular, psychological, socioeconomic, and clinical biomarkers of ECT response in TRD...
August 30, 2023: European Archives of Psychiatry and Clinical Neuroscience
https://read.qxmd.com/read/37640804/integrated-computational-screening-and-liquid-biopsy-approach-to-uncover-the-role-of-biomarkers-for-oral-cancer-lymph-node-metastasis
#25
JOURNAL ARTICLE
Shayma Shaikh, Deep Kumari Yadav, Kinjal Bhadresha, Rakesh M Rawal
Cancer is an abnormal, heterogeneous growth of cells with the ability to invade surrounding tissue and even distant organs. Worldwide, GLOBOCAN had an estimated 18.1 million new cases and 9.6 million death rates of cancer in 2018. Among all cancers, Oral cancer (OC) is the sixth most common cancer worldwide, and the third most common in India, the most frequent type, oral squamous cell carcinoma (OSCC), tends to spread to lymph nodes in advanced stages. Throughout the past few decades, the molecular landscape of OSCC biology has remained unknown despite breakthroughs in our understanding of the genome-scale gene expression pattern of oral cancer particularly in lymph node metastasis...
August 28, 2023: Scientific Reports
https://read.qxmd.com/read/37624588/designing-multimodal-informative-sensing-with-an-exosome-mediated-signal-coupling-transduction-strategy-based-on-a-single-stimulus-multiresponse-recognition-interface
#26
JOURNAL ARTICLE
Yitian Huang, Shupei Zhang, Yanjie Chen, Lihong Gao, Hong Dai
Given that exosomes released from cancer cells carry various tumor-specific proteins on their surface, they have emerged as a source of biomarkers for cancer diagnosis. However, developing accurate and reliable assays to detect exosomes in the early stages of disease with low abundance and complex systems remains challenging. Here, the prepared PDIG film has the ability to sense multiple signals from a single stimulus, in which the presence of cobalt(II) chloride and deep eutectic solvents (DES) endows PDIG with thermochromic and thermosensitive properties...
August 25, 2023: Analytical Chemistry
https://read.qxmd.com/read/37614965/cellular-senescence-primes-liver-fibrosis-regression-through-notch-ezh2
#27
JOURNAL ARTICLE
Ping Song, Juan-Li Duan, Jian Ding, Jing-Jing Liu, Zhi-Qiang Fang, Hao Xu, Zhi-Wen Li, Wei Du, Ming Xu, Yu-Wei Ling, Fei He, Kai-Shan Tao, Lin Wang
Cellular senescence plays a pivotal role in wound healing. At the initiation of liver fibrosis regression, accumulated senescent cells were detected and genes of senescence were upregulated. Flow cytometry combined with single-cell RNA sequencing analyses revealed that most of senescent cells were liver nonparenchymal cells. Removing senescent cells by dasatinib and quercetin (DQ), alleviated hepatic cellular senescence, impeded fibrosis regression, and disrupted liver sinusoids. Clearance of senescent cells not only decreased senescent macrophages but also shrank the proportion of anti-inflammatory M2 macrophages through apoptotic pathway...
October 2023: MedComm
https://read.qxmd.com/read/37609432/an-exosome-based-therapeutic-strategy-targeting-neuroinflammation-in-alzheimer-s-disease-with-berberine-and-palmatine
#28
JOURNAL ARTICLE
Xin Zhao, Pingyuan Ge, Shaohua Lei, Siqi Guo, Peng Zhou, Li Zhao, Yiyu Qi, Xiaotong Wei, Weizhen Wu, Ningjing Wang, Rui Guo, Nianyun Yang, Qingqing Xiao, Qichun Zhang, Huaxu Zhu
INTRODUCTION: Neuroinflammation is one of the major pathogeneses in Alzheimer's disease (AD) and mainly involves abnormal inflammatory activation of microglia by multiple pathological stimuli. The treatment of AD remains a major challenge due to the multifactorial characterization of AD and the inefficient ability of therapeutic drugs to permeate through the blood‒brain barrier (BBB). Accordingly, drug combination treatment and drug carrier delivery have become important therapeutic tools for the treatment of multifactorial diseases, especially AD...
2023: Drug Design, Development and Therapy
https://read.qxmd.com/read/37598634/virological-characterization-of-hiv-1-rna-elements-detected-exclusively-through-the-ltr-region-by-the-dual-target-aptima-hiv-1-quant-dx-assay-in-a-subset-of-positive-patients
#29
JOURNAL ARTICLE
Giuseppe Sberna, Roberta Nardacci, Giulia Berno, Gabriella Rozera, Emanuela Giombini, Lavinia Fabeni, Eliana Specchiarello, Fabrizio Maggi, Alessandra Amendola
BACKGROUND: In a restricted subset of HIV patients with suppressed viral load (i.e., pol-undetected HIV-RNA), the Aptima HIV-1 Quant Dx Assay (Aptima), a dual-target (pol and LTR) and dual-probe test for viral load (VL) monitoring, can detect HIV-RNA exclusively through amplification of the LTR region. OBJECTIVES: To analyze the virological characteristics of the HIV-RNA elements detected only through LTR amplification (LTR-e). STUDY DESIGN: LTR-e isolated from plasma and peripheral blood mononuclear cells (PBMC) were evaluated for their ability to trigger productive infections...
August 15, 2023: Journal of Clinical Virology
https://read.qxmd.com/read/37567507/quantitative-and-functional-characterisation-of-extracellular-vesicles-after-passive-loading-with-hydrophobic-or-cholesterol-tagged-small-molecules
#30
JOURNAL ARTICLE
Gwenola Tréton, Claudia Sayer, Melanie Schürz, Maria Jaritsch, Anna Mueller, Cristian-Tudor Matea, Vesna Stanojlovic, Heloisa Melo-Benirschke, Celine Be, Caroline Krembel, Stephane Rodde, Matthias Haffke, Samuel Hintermann, Andreas Marzinzik, Sébastien Ripoche, Constantin Blöchl, Julia Hollerweger, Daniela Auer, Chiara Cabrele, Christian G Huber, Martin Hintersteiner, Trixie Wagner, Andreas Lingel, Nicole Meisner-Kober
Extracellular vesicles (EVs) are nanosized intercellular messengers that bear enormous application potential as biological drug delivery vehicles. Much progress has been made for loading or decorating EVs with proteins, peptides or RNAs using genetically engineered donor cells, but post-isolation loading with synthetic drugs and using EVs from natural sources remains challenging. In particular, quantitative and unambiguous data assessing whether and how small molecules associate with EVs versus other components in the samples are still lacking...
August 9, 2023: Journal of Controlled Release
https://read.qxmd.com/read/37540442/multiplex-immunostaining-method-to-distinguish-hsp-isoforms-in-cancer-tissue-specimens
#31
JOURNAL ARTICLE
Hotaka Kawai, Kisho Ono, Takanori Eguchi
Heat shock proteins (HSPs) are often expressed in all nucleated cells, but their expression profiles differ. In particular, HSP90α and HSP90β have high sequence identity and have not been fully examined for their individual and compensatory functions as molecular chaperones, differences in client proteins, and extracellular distributions with exosomes. Immunohistochemical staining is a technique to visualize the presence and localization of target antigens using specific antibodies, of which the multiplex immunostaining method can reveal differences in protein expression in the same tumor tissue and the localization of proteins of interest within tumor tissue or single cells...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/37511901/lessons-from-polyomavirus-immunofluorescence-staining-of-urinary-decoy-cells
#32
JOURNAL ARTICLE
Sahra Pajenda, Zsofia Hevesi, Michael Eder, Daniela Gerges, Monika Aiad, Oliver Koldyka, Wolfgang Winnicki, Ludwig Wagner, Farsad Eskandary, Alice Schmidt
Decoy cells that can be detected in the urine sediment of immunosuppressed patients are often caused by the uncontrolled replication of polyomaviruses, such as BK-Virus (BKV) and John Cunningham (JC)-Virus (JCV), within the upper urinary tract. Due to the wide availability of highly sensitive BKV and JCV PCR, the diagnostic utility of screening for decoy cells in urine as an indicator of polyomavirus-associated nephropathy (PyVAN) has been questioned by some institutions. We hypothesize that specific staining of different infection time-dependent BKV-specific antigens in urine sediment could allow cell-specific mapping of antigen expression during decoy cell development...
July 7, 2023: Life
https://read.qxmd.com/read/37483631/expression-of-targets-of-the-rna-binding-protein-auf-1-in-human-airway-epithelium-indicates-its-role-in-cellular-senescence-and-inflammation
#33
JOURNAL ARTICLE
Ilaria Salvato, Luca Ricciardi, Jessica Dal Col, Annunziata Nigro, Giorgio Giurato, Domenico Memoli, Assunta Sellitto, Erwin Pavel Lamparelli, Maria Assunta Crescenzi, Monica Vitale, Alessandro Vatrella, Francesco Nucera, Paola Brun, Federico Caicci, Paola Dama, Thomas Stiff, Leandro Castellano, Sobia Idrees, Matt D Johansen, Alen Faiz, Peter A Wark, Philip M Hansbro, Ian M Adcock, Gaetano Caramori, Cristiana Stellato
INTRODUCTION: The RNA-binding protein AU-rich-element factor-1 (AUF-1) participates to posttranscriptional regulation of genes involved in inflammation and cellular senescence, two pathogenic mechanisms of chronic obstructive pulmonary disease (COPD). Decreased AUF-1 expression was described in bronchiolar epithelium of COPD patients versus controls and in vitro cytokine- and cigarette smoke-challenged human airway epithelial cells, prompting the identification of epithelial AUF-1-targeted transcripts and function, and investigation on the mechanism of its loss...
2023: Frontiers in Immunology
https://read.qxmd.com/read/37478050/amperometric-identification-of-single-exosomes-and-their-dopamine-contents-secreted-by-living-cells
#34
JOURNAL ARTICLE
Jian Lv, Xiao-Yuan Wang, Shuai Chang, Cheng-Ye Xi, Xue Wu, Bin-Bin Chen, Zhi-Qian Guo, Da-Wei Li, Ruo-Can Qian
Dopamine (DA) is an important neurotransmitter, which not only participates in the regulation of neural processes but also plays critical roles in tumor progression and immunity. However, direct identification of DA-containing exosomes, as well as quantification of DA in single vesicles, is still challenging. Here, we report a nanopipette-assisted method to detect single exosomes and their dopamine contents via amperometric measurement. The resistive-pulse current measured can simultaneously provide accurate information of vesicle translocation and DA contents in single exosomes...
July 21, 2023: Analytical Chemistry
https://read.qxmd.com/read/37405532/establishment-and-evaluation-of-exosomes-related-gene-risk-model-in-hepatocellular-carcinoma
#35
JOURNAL ARTICLE
Lin Zhu, Yan Lou, Qiyu Xiao, Ling Wang, Guodong Chen, Wenjun Yang, Tengjiao Wang
Hepatocellular carcinoma (HCC) is a challenging disease to evaluate in terms of prognosis, requiring close attention to the prognosis of HCC patients. Exosomes have been shown to play an important role in HCC development and have significant potential in managing HCC patient prognosis, as they are detectable in patients' blood. By using small extracellular vesicular RNA, liquid biopsies can reflect the underlying physiological and pathological status of the originating cells, providing a valuable assessment of human health...
July 5, 2023: Biochemical Genetics
https://read.qxmd.com/read/37368982/real-time-monitoring-of-exosomes-secretion-from-single-cell-using-dual-nanopore-biosensors
#36
JOURNAL ARTICLE
Hongshuai Zhang, Xin Zheng, Tao Zhao, Yiping Chen, Yang Luo, Yangcan Dong, Hao Tang, Jianhui Jiang
Exosomes secreted from cells carry rich information from their parent cells, representing a promising biomarker for investigation of diseases. We develop a dual-nanopore biosensor using DNA aptamers to specifically recognize CD63 protein on the exosome's surface, which enables label-free exosome detection based on ionic current change. The sensor allows for sensitive detection of exosomes with a detection limit of 3.4 × 106 particles/mL. The dual-nanopore biosensor was able to form an intrapipette electric circuit for ionic current measurement due to its unique structure, which is crucial to achieve detection of exosome secretion from a single cell...
June 27, 2023: ACS Sensors
https://read.qxmd.com/read/37352015/exosomal-mirnas-and-breast-cancer-a-complex-theranostics-interlink-with-clinical-significance
#37
REVIEW
Sayantanee Mukherjee, Rajib Dhar, Swathi Jonnalagadda, Sukhamoy Gorai, Sagnik Nag, Rishav Kar, Nobendu Mukerjee, Dattatreya Mukherjee, Rishabh Vatsa, Devi Arikketh, Anand Krishnan, Rohit Gundamaraju, Saurabh Kumar Jha, Athanasios Alexiou, Marios Papadakis
Breast cancer (BC) remains the most challenging global health crisis of the current decade, impacting a large population of females annually. In the field of cancer research, the discovery of extracellular vesicles (EVs), specifically exosomes (a subpopulation of EVs), has marked a significant milestone. In general, exosomes are released from all active cells but tumor cell-derived exosomes (TDXs) have a great impact (TDXs miRNAs, proteins, lipid molecules) on cancer development and progression. TDXs regulate multiple events in breast cancer such as tumor microenvironment remodeling, immune cell suppression, angiogenesis, metastasis (EMT-epithelial mesenchymal transition, organ-specific metastasis), and therapeutic resistance...
June 23, 2023: Biomarkers: Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals
https://read.qxmd.com/read/37314731/integrated-lab-on-a-chip-microfluidic-systems-for-isolation-enrichment-and-analysis-of-cancer-biomarkers
#38
REVIEW
Sushruta Surappa, Priyanka Multani, Ugur Parlatan, Prima Dewi Sinawang, Jussuf Kaifi, Demir Akin, Utkan Demirci
The liquid biopsy has garnered considerable attention as a complementary clinical tool for the early detection, molecular characterization and monitoring of cancer over the past decade. In contrast to traditional solid biopsy techniques, liquid biopsy offers a less invasive and safer alternative for routine cancer screening. Recent advances in microfluidic technologies have enabled handling of liquid biopsy-derived biomarkers with high sensitivity, throughput, and convenience. The integration of these multi-functional microfluidic technologies into a 'lab-on-a-chip' offers a powerful solution for processing and analyzing samples on a single platform, thereby reducing the complexity, bio-analyte loss and cross-contamination associated with multiple handling and transfer steps in more conventional benchtop workflows...
June 28, 2023: Lab on a Chip
https://read.qxmd.com/read/37291937/latest-advances-and-perspectives-of-liquid-biopsy-for-cancer-diagnostics-driven-by-microfluidic-on-chip-assays
#39
REVIEW
Yujiao Xie, Xiawei Xu, Jing Wang, Jie Lin, Yong Ren, Aiguo Wu
Microfluidic-based lab-on-a-chip technology is a multidisciplinary approach, which has evolved rapidly in the past decade and remains a hot research topic as a promising microanalysis platform for a plethora of biomedical applications. Microfluidic chips have been successfully applied in cancer diagnosis and monitoring, given that they can lead to the effective separation and analysis of cancer-derived substances such as extracellular vesicles (EVs), circulating tumour cells (CTCs) and circulating DNA (ctDNA), proteins and other metabolites...
June 9, 2023: Lab on a Chip
https://read.qxmd.com/read/37284418/preventing-swarm-detection-in-extracellular-vesicle-flow-cytometry-a-clinically-applicable-procedure
#40
JOURNAL ARTICLE
Naomi C Buntsma, Mona Shahsavari, Aleksandra Gąsecka, Rienk Nieuwland, Ton G van Leeuwen, Edwin van der Pol
BACKGROUND: Flow cytometry is commonly used to detect cell-derived extracellular vesicles in body fluids such as blood plasma. However, continuous and simultaneous illumination of multiple particles at or below the detection limit may result in the detection of a single event. This phenomenon is called swarm detection and leads to incorrect particle concentration measurements. To prevent swarm detection, sample dilution is recommended. Since the concentration of particles differs between plasma samples, finding the optimal sample dilution requires dilution series of all samples, which is unfeasible in clinical routine...
May 2023: Research and Practice in Thrombosis and Haemostasis
keyword
keyword
171110
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.