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Colorectal Cancer And MiRNA

Huizhu Gan, Lin Lin, Nanjun Hu, Yang Yang, Yu Gao, Yu Pei, Kang Chen, Butong Sun
BACKGROUND: Aspirin, an anti-inflammatory drug, has been widely investigated in the treatment of many cancer types, including colorectal, ovarian, breast, and prostate cancers. MicroRNAs (miRNAs) are the most well studied noncoding RNAs in cancers. In the current study, we were interested in defining the function of aspirin in lung cancer treatment and the related noncoding RNAs involved in this process. METHODS: The function of aspirin in lung cancer growth was evaluated by cell viability and colony formation assays...
February 12, 2019: Thoracic Cancer
Qian Liu, Junjie Deng, Xundong Wei, Wei Yuan, Jie Ma
Colorectal cancer (CRC) is a common malignant tumor with high morbidity and mortality around the world. The aim of this study was to determine the genes significantly associated with the overall survival (OS) of CRC patients and predict their function in the competing endogenous RNA (ceRNA) regulation networks. We constructed the lncRNA-miRNA-mRNA networks according to the differentially expressed RNAs from the The Cancer Genome Atlas data sets of 561 CRC patients. Twelve differentially expressed messenger RNAs from the ceRNA networks were selected through a univariate Cox proportional hazards regression...
February 12, 2019: Journal of Cellular Biochemistry
Xiaoxiang Chen, Kaixuan Zeng, Mu Xu, Xiangxiang Liu, Xiuxiu Hu, Tao Xu, Bangshun He, Yuqin Pan, Huiling Sun, Shukui Wang
MicroRNAs (miRNAs) are important class of functional regulators involved in human cancers development, including colorectal cancer (CRC). Exploring aberrantly expressed miRNAs may provide us with new insights into the initiation and development of CRC by functioning as oncogenes or tumor suppressors. The aim of our study is to discover the expression pattern of miR-1249 in CRC and investigate its clinical significance as well as biological role in CRC progression. In our study, we found that miR-1249 was markedly downregulated in CRC tissues and cell lines, and negatively related to pN stage, pM stage, TNM stage, and overall survival (OS)...
February 12, 2019: Cell Death & Disease
Zhanwei Xuan, Jiechen Li, Jingwen Yu, Xiang Feng, Bihai Zhao, Lei Wang
Recently, an increasing number of studies have indicated that long-non-coding RNAs (lncRNAs) can participate in various crucial biological processes and can also be used as the most promising biomarkers for the treatment of certain diseases such as coronary artery disease and various cancers. Due to costs and time complexity, the number of possible disease-related lncRNAs that can be verified by traditional biological experiments is very limited. Therefore, in recent years, it has been very popular to use computational models to predict potential disease-lncRNA associations...
February 8, 2019: Genes
Shimeng Pan, Yanqiu Liu, Qianqian Liu, Yang Xiao, Bing Liu, Xiang Ren, Xia Qi, Huimin Zhou, Changqian Zeng, Li Jia
Metastasis is the main cause of death in colorectal cancer (CRC) patients. Aberrant fucosylation, catalyzed by the specific fucosyltransferases (FUTs), is associated with malignant behaviors. Non-conding RNAs, including long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), emerge as key molecules in cancer malignancy. The aim of this study was to investigate HOTAIR/miR-326/FUT6 axis modified fucosylation on sLeX -CD44 (HCELL), which served as E-selectin ligand during CRC progression. Higher levels of HOTAIR and FUT6 were verified in CRC tissues and cell lines, with a positive correlation...
February 8, 2019: Biochimica et biophysica acta. Molecular cell research
Stergios Boussios, Mehmet Akif Ozturk, Michele Moschetta, Afroditi Karathanasi, Nikolaos Zakynthinakis-Kyriakou, Konstantinos H Katsanos, Dimitrios K Christodoulou, Nicholas Pavlidis
Colorectal cancer (CRC) is the third most common malignancy worldwide. Surgery remains the most important treatment for non-metastatic CRC, and the administration of adjuvant chemotherapy depends mainly on the disease stage, which is still the strongest prognostic factor. A refined understanding of the genomics of CRC has recently been achieved thanks to the widespread use of next generation sequencing with potential future therapeutic implications. Microsatellite instability (MSI) has been suggested as a predictive marker for response to anti-programmed-cell-death protein 1 (PD-1) therapy in solid tumors, including CRC...
February 7, 2019: Journal of Personalized Medicine
Jingwei Liu, Hao Li, Bowen Zheng, Liping Sun, Yuan Yuan, Chengzhong Xing
BACKGROUND: Competitive endogenous RNA (ceRNA) regulation suggested complex network of all transcript RNAs including long noncoding RNAs (lncRNAs), which can act as natural miRNA sponges to inhibit miRNA functions and modulate mRNA expression. Until now, the specific ceRNA regulatory mechanism of lncRNA-miRNA-mRNA in colorectal cancer (CRC) still remains unclear. MATERIALS AND METHODS: RNA sequencing data of 478 colon adenocarcinoma cases and 41 controls as well as 166 rectum adenocarcinoma cases and 10 controls were obtained from The Cancer Genome Atlas (TCGA) to investigate the significant changes of lncRNAs, miRNAs and mRNAs in colorectal carcinogenesis...
February 7, 2019: Digestive Diseases and Sciences
J-J Song, W Li
OBJECTIVE: MicroRNA (miRNA) plays important roles in the progression of different cancers. In this study, we investigated the precise role of miR-10b in the growth and metastasis of colorectal cancer (CRC) cell. PATIENTS AND METHODS: The levels of miR-10b in CRC cell lines were detected using quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) assay. A series of functional assays, including cell proliferation, colony formation, wound healing and transwell invasion were conducted using miR-10b transfected cells...
January 2019: European Review for Medical and Pharmacological Sciences
Marta Herreros-Villanueva, Saray Duran-Sanchon, Ana Carmen Martín, Rosa Pérez-Palacios, Elena Vila-Navarro, María Marcuello, Mireia Diaz-Centeno, Joaquín Cubiella, Maria Soledad Diez, Luis Bujanda, Angel Lanas, Rodrigo Jover, Vicent Hernández, Enrique Quintero, Juan José Lozano, Marta García-Cougil, Ibon Martínez-Arranz, Antoni Castells, Meritxell Gironella, Rocio Arroyo
OBJECTIVES: Specific microRNA (miRNA) signatures in biological fluids can facilitate earlier detection of the tumors being then minimally invasive diagnostic biomarkers. Circulating miRNAs have also emerged as promising diagnostic biomarkers for colorectal cancer (CRC) screening. In this study, we investigated the performance of a specific signature of miRNA in plasma samples to design a robust predictive model that can distinguish healthy individuals from those with CRC or advanced adenomas (AA) diseases...
January 2019: Clinical and Translational Gastroenterology
Parinaz Nasri Nasrabadi, Somaye Zareian, Zahra Nayeri, Reza Salmanipour, Soha Parsafar, Ehsan Gharib, Hamid Asadzadeh Aghdaei, Mohammad Reza Zali
Natural dietary ingredients like flavonoids are important for body improvement against diseases. The flavonol rutin is widely found in fruits and vegetables and shows significant anticancer properties. However, the underlined signaling pathways have not been elucidated yet. In this study, the impacts of various doses of rutin (400-700 mM/ml) have been examined on human colon cancer SW480 cells metabolism, cell cycle, and apoptosis. The transcriptome was analyzed by bioinformatics tools and the interactions between rutin modulated microRNAs (miRNAs), long noncoding RNAs (lncRNAs), messenger RNAs (mRNAs), and transcription factors (TFs) were built, filtered and enriched...
January 29, 2019: Journal of Cellular Physiology
Marta Schirripa, Beatrice Borelli, Romina D'Aurizio, Simone Lubrano, Chiara Cremolini, Gemma Zucchelli, Carlotta Antoniotti, Federica Marmorino, Alessandra Anna Prete, Sabina Murgioni, Francesca Bergamo, Vittorina Zagonel, Andrea Tuccoli, Andrea Marranci, Milena Rizzo, Lorena Tedeschi, Letizia Magnoni, Alfredo Falcone, Fotios Loupakis, Laura Poliseno
Biomarkers able to improve the cost/benefit ratio are urgently needed for metastatic colorectal cancer patients that are eligible to receive regorafenib. Here, we measured plasma levels of ten circulating microRNAs (c-miRNAs) and we investigated their early changes during treatment, as well as possible correlation with clinical outcome. Ten literature-selected c-miRNAs were quantified by qRT-PCR on plasma samples collected at baseline (d1) and after 15 days of treatment (d15). C-miRNAs showing significant changes were further analyzed to establish correlations with outcome...
January 28, 2019: Pharmacogenomics Journal
Yuxi Qi, Jinliang Li
BACKGROUND: Triptolide (TPL) is the active component of Tripterygium wifordii Hook F, which has been reported to exert anti-tumor efficacies. Herein, we aimed to examine the efficacy of TPL in colorectal cancer (CRC) cells and to reveal its underlying mechanisms. METHODS: Human CRC cell lines HT-29 and SW480 were treated by TPL for 24 h in the presence or absence of epithelial-to-mesenchymal transition (EMT) inducer TGF-β1. The expression of miR-191 in cell was overexpressed by miRNA transfection...
January 23, 2019: Experimental and Molecular Pathology
Atena Soleimani, Majid Khazaei, Gordon A Ferns, Mikhail Ryzhikov, Amir Avan, Seyed Mahdi Hassanian
Transforming growth factor β (TGF-β) modulates tumor progression by regulating cell proliferation, apoptosis, metastasis, angiogenesis, and drug resistance. Biological and pharmacological agonists/antagonists, the interplay between intracellular signaling pathways, and microRNAs (miRNAs) control the initiation and activation of the TGF-β signaling pathway. It has been proposed that the expression profiles of tumor suppressor and oncogenic TGF-β miRNAs may be used for the classification, diagnosis, and prognosis of human malignancies...
January 26, 2019: Journal of Cellular Physiology
Xinfeng Yu, Dong Wang, Xiaohui Wang, Shiyue Sun, Yuhang Zhang, Shuqing Wang, Rongrong Miao, Xiaoxue Xu, Xianjun Qu
BACKGROUND: Activation of CXCL12/CXCR4 axis has been found to be associated with invasion and metastasis in many cancers. However, the underlying mechanism remains elusive. Increasing data highlight that non-coding RNAs are linked to CRC progression. METHODS: The effects of CXCR4 were investigated using villin-CXCR4 transgenic mice model by flow cytometry assay, immunohistochemistry, and Western blot. The mechanism was explored through bioinformatics, luciferase reporter assay and RNA immunoprecipitation assay...
January 24, 2019: Journal of Experimental & Clinical Cancer Research: CR
Ce Yuan, Subbaya Subramanian
Worldwide, colorectal cancer (CRC) is one of the leading causes of cancer-related deaths. Recent advances in high-throughput technologies have shown that the gut microbiota may have a major influence on human health, including CRC. Nonetheless, how the gut microbiota interacts with tumor cells in CRC patients is largely unknown. Studies have shown that the microbiota fills in a variety of niche metabolic pathways that the host does not possess. For example, the microbiota produces butyrate, which provides the colon's epithelial cells with about 70% of their energy needs...
January 22, 2019: DNA and Cell Biology
Xiangxiang Liu, Xueni Xu, Bei Pan, Bangshun He, Xiaoxiang Chen, Kaixuan Zeng, Mu Xu, Yuqin Pan, Huiling Sun, Tao Xu, Xiuxiu Hu, Shukui Wang
Background : The lack of screening methods with high diagnostic utility leads to colorectal cancer (CRC) patients usually diagnosed in advanced stages which results in high mortality. This study aimed to identify novel circulating miRNAs as biomarkers for the early detection of CRC. Materials and Methods : Total 205 participants were enrolled in this study. First, two dysregulated candidate miRNAs were selected after integrated analysis of four GEO datasets. Then, the expression of these two miRNAs in plasma samples were tested through qRT-PCR...
2019: Journal of Cancer
Md Rezanur Rahman, Tania Islam, Esra Gov, Beste Turanli, Gizem Gulfidan, Md Shahjaman, Nilufa Akhter Banu, Md Nurul Haque Mollah, Kazim Yalcin Arga, Mohammad Ali Moni
Colorectal cancer (CRC) is the second most common cause of cancer-related death in the world, but early diagnosis ameliorates the survival of CRC. This report aimed to identify molecular biomarker signatures in CRC. We analyzed two microarray datasets (GSE35279 and GSE21815) from the Gene Expression Omnibus (GEO) to identify mutual differentially expressed genes (DEGs). We integrated DEGs with protein⁻protein interaction and transcriptional/post-transcriptional regulatory networks to identify reporter signaling and regulatory molecules; utilized functional overrepresentation and pathway enrichment analyses to elucidate their roles in biological processes and molecular pathways; performed survival analyses to evaluate their prognostic performance; and applied drug repositioning analyses through Connectivity Map (CMap) and geneXpharma tools to hypothesize possible drug candidates targeting reporter molecules...
January 17, 2019: Medicina
Wei Wang, Ying He, Jing Rui, Mao-Qi Xu
Colorectal cancer (CRC) is a common malignancy with high morbidity. MicroRNAs (miRNAs or miRs) have been demonstrated to be critical post-transcriptional regulators in tumorigenesis. The current study aimed to investigate the effect of miR-410 on the proliferation and metastasis of CRC. The expression of miR-410 was examined in CRC cell lines. SW-480 and HCT-116 CRC cell lines were employed and transfected with miR-410 inhibitor or miR-410 mimics. The association between miR-410 and dickkopf-related protein 1 (DKK-1) was verified by luciferase reporter assay...
January 2019: Oncology Letters
Limei Zhu, Ran Wang, Li Zhang, Chunlei Zuo, Rui Zhang, Shaolin Zhao
Background: Previous research indicated that overexpression of miRNA-211 could promote colorectal cancer cell growth by targeting tumor suppressive gene Chromodomain-helicase-DNA-binding protein 5 (CHD5) in human colon cancer (CC). Moreover, the function of the single-nucleotide polymorphism (SNP) located in the mature region of miR-211 has not been investigated. In this study, we found that SNP of rs187960998 in miR-211 was involved in the occurrence of CC by acting as a tumor suppressor by mal-regulation of its target gene CHD5 ...
2019: OncoTargets and Therapy
Shan Muhammad, Qingchao Tang, Liu Wei, Qian Zhang, Guiyu Wang, Bilal Umar Muhammad, Kavanjit Kaur, Tatiana Kamchedalova, Zhao Gang, Zheng Jiang, Zheng Liu, Xishan Wang
MicroRNAs (miRNAs or miRs) are reported to be dysregulated in the progression and invasion of various human cancer types, including colorectal cancer (CRC). They are also reported to be molecular biomarkers and therapeutic targets in CRC. miRNAs serve functions in a plethora of biological processes, including proliferation, migration, invasion and apoptosis, and several miRNAs have been demonstrated to be involved in CRC carcinogenesis, invasion and metastasis. Aberrant miR-30d expression and its effects have been reported in certain cancer types...
January 2019: Experimental and Therapeutic Medicine
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