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tgfb follicle

Yang Gao, Xin Fang, David F Vincent, David W Threadgill, Laurent Bartholin, Qinglei Li
BACKGROUND: Transforming growth factor beta (TGFB) superfamily signaling is implicated in the development of sex cord-stromal tumors, a category of poorly defined gonadal tumors. The aim of this study was to determine potential effects of dysregulated TGFB signaling in the ovary using Cre recombinase driven by growth differentiation factor 9 (Gdf9) promoter known to be expressed in oocytes. METHODS: A mouse model containing constitutively active TGFBR1 (TGFBR1CA ) using Gdf9-iCre (termed TGFBR1-CAG9Cre ) was generated...
December 8, 2017: Reproductive Biology and Endocrinology: RB&E
Yi-Lin Yan, Thomas Desvignes, Ruth Bremiller, Catherine Wilson, Danielle Dillon, Samantha High, Bruce Draper, Charles Loren Buck, John Postlethwait
BACKGROUND: Aberrant signaling between germ cells and somatic cells can lead to reproductive disease and depends on diffusible signals, including transforming growth factor-beta (TGFB) -family proteins. The TGFB-family protein Gsdf (gonadal soma derived factor) controls sex determination in some fish and is a candidate for mediating germ cell/soma signaling. RESULTS: Zebrafish expressed gsdf in somatic cells of bipotential gonads and expression continued in ovarian granulosa cells and testicular Sertoli cells...
November 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
W Kranc, J Budna, R Kahan, A Chachuła, A Bryja, S Ciesiółka, S Borys, M P Antosik, D Bukowska, K P Brussow, M Bruska, M Nowicki, M Zabel, B Kempisty
For normal folliculogenesis and oogenesis to occur many intrinsic and extrinsic factors are needed, i.e. positive feedback of hormone secretion and local ovarian-follicular growth factors distribution. During follicle formation, granulosa cells (GCs) change their morphology and physiological properties. The factors needed for GCs to differentiate within each layer are transforming growth factor beta (TGFB) and insulin-like growth factor (IGF), as well as the activation and modification of biochemical pathways involved in folliculogenesis...
January 2017: Journal of Biological Regulators and Homeostatic Agents
Isam B Sharum, Sofia Granados-Aparici, Fiona C Warrander, Felicity P Tournant, Mark A Fenwick
The molecular mechanisms involved in regulating the development of small, gonadotrophin-independent follicles are poorly understood; however, many studies have highlighted an essential role for TGFB ligands. Canonical TGFB signalling is dependent upon intracellular SMAD proteins that regulate transcription. STRAP has been identified in other tissues as an inhibitor of the TGFB-SMAD signalling pathway. Therefore, in this study we aimed to determine the expression and role of STRAP in the context of early follicle development...
February 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
Vahid Zadmajid, Ali Falahatimarvast, Erin L Damsteegt, Alvin N Setiawan, Yuichi Ozaki, Alireza Shoae, P Mark Lokman
Members of the transforming growth factor-b (TGFb) superfamily are important during early oogenesis in mammals. In this study, we tested whether documented effects of 11-ketotestosterone (11KT) on previtellogenic eel ovaries are mediated through affecting the expression of key ovarian TGFb genes. Furthermore, we investigated whether 11KT effects interacted with temperature. Accordingly, three thermal regimes were compared and their interaction with 11KT-mediated actions on expression of TGFb superfamily genes (chiefly inhibin subunits) evaluated in the eel (Anguilla australis)...
September 2015: Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
V Matiller, M L Stangaferro, P U Díaz, H H Ortega, F Rey, E Huber, N R Salvetti
Cystic ovarian disease (COD) is one of the main causes of infertility in dairy cattle. It has been shown that intra-ovarian factors may contribute to follicular persistence. Transforming growth factor-beta (TGFB) isoforms are important paracrine and autocrine signalling molecules that regulate ovarian follicle growth and physiology. Considering the importance of these factors in the ovarian physiology, in this study, we examined the expression of TGFB isoforms (TGFB1, TGFB2 and TGFB3) in the ovary of healthy cows and animals with spontaneous and adrenocorticotrophic hormone (ACTH)-induced COD...
October 2014: Reproduction in Domestic Animals
Amanda Feeney, Eric Nilsson, Michael K Skinner
An ovarian follicle is composed of an oocyte and surrounding theca and granulosa cells. Oocytes are stored in an arrested state within primordial follicles until they are signaled to re-initiate development by undergoing primordial-to-primary follicle transition. Previous gene bionetwork analyses of primordial follicle development identified a number of critical cytokine signaling pathways and genes potentially involved in the process. In the current study, candidate regulatory genes and pathways from the gene network analyses were tested for their effects on the formation of primordial follicles (follicle assembly) and on primordial follicle transition using whole ovary organ culture experiments...
September 2014: Reproduction: the Official Journal of the Society for the Study of Fertility
Juliano C da Silveira, Elaine M Carnevale, Quinton A Winger, Gerrit J Bouma
BACKGROUND: Ovarian follicle growth and maturation requires extensive communication between follicular somatic cells and oocytes. Recently, intercellular cell communication was described involving cell-secreted vesicles called exosomes (50-150 nm), which contain miRNAs and protein, and have been identified in ovarian follicular fluid. The goal of this study was to identify a possible role of exosomes in follicle maturation. METHODS: Follicle contents were collected from mares at mid-estrous (~35 mm, before induction of follicular maturation) and pre-ovulatory follicles (30-34 h after induction of follicular maturation)...
2014: Reproductive Biology and Endocrinology: RB&E
Marta Jackowska, Bartosz Kempisty, Magdalena Woźna, Hanna Piotrowska, Paweł Antosik, Piotr Zawierucha, Dorota Bukowska, Michał Nowicki, Jędrzej M Jaśkowski, Klaus-Peter Brüssow
The TGFB superfamily genes are involved in several important cell functions, including proliferation and differentiation, and the role of the expression of these genes in growth and development of theca and granulosa cells is well recognised. However, the dependence between the stage of oocyte maturation or follicular size and the expression of these genes in pigs is still not entirely known. This study was aimed at investigating the expression pattern of GDF9, TGFB1, TGFB2 and TGFB3 in porcine oocytes before and after in vitro maturation (IVM) as well as in oocytes collected from follicles of different sizes...
March 2013: Acta Veterinaria Hungarica
Elisabeth Sambroni, Antoine D Rolland, Jean-Jacques Lareyre, Florence Le Gac
The general rules established from mammalian species for the regulation of spermatogenesis by gonadotropins may not be fully relevant in fish. Particularly, Fsh is as potent as Lh to stimulate steroidogenesis and the Fsh receptor is expressed in Leydig cells. In seasonal breeders, Fsh is likely the major gonadotropin involved in spermatogenesis onset and Lh is required to support spermatogenesis progression and gamete release. However, the genes that relay the action of Fsh and Lh have been poorly investigated in fish...
February 2013: Journal of Molecular Endocrinology
Stephanie A Pangas
Genetic or environmental factors that affect the endowment of oocytes, their assembly into primordial follicles, or their subsequent entry into the growing follicle pool can disrupt reproductive function and may underlie disorders such as primary ovarian insufficiency. Mouse models have been instrumental in identifying genes important in ovarian development, and a number of genes now associated with ovarian dysfunction in women were first identified as causing reproductive defects in knockout mice. The transforming growth factor beta (TGFB) family consists of developmentally important growth factors that include the TGFBs, anti-Müllerian hormone (AMH), activins, bone morphogenetic proteins (BMPs), and growth and differentiation factor 9 (GDF9)...
October 2012: Molecular Reproduction and Development
Chitra Joseph, Morag G Hunter, Kevin D Sinclair, Robert S Robinson
The role of the tissue remodelling protein, secreted protein, acidic, cysteine-rich (SPARC), in key processes (e.g. cell reorganisation and angiogenesis) that occur during the follicle-luteal transition is unknown. Hence, we investigated the regulation of SPARC in luteinsing follicular cells and potential roles of SPARC peptide 2.3 in a physiologically relevant luteal angiogenesis culture system. SPARC protein was detected mainly in the theca layer of bovine pre-ovulatory follicles, but its expression was considerably greater in the corpus haemorrhagicum...
September 2012: Reproduction: the Official Journal of the Society for the Study of Fertility
Zhi He, Yangsheng Wu, Jun Xie, Taixin Wang, Lihong Zhang, Weimin Zhang
Growth differentiation factor 9 (GDF9) is a member of the transforming growth factor beta (TGFb) superfamily. As an oocyte-derived growth factor, GDF9 plays key roles in regulating follicle development. In the present study, we identified a gdf9 homologue from the ovary of ricefield eel, and analyzed its expression both at the mRNA and protein levels. Ricefield eel Gdf9 showed high homologies with those of other teleosts, especially perciformes fish. RT-PCR analysis revealed that ricefield eel gdf9 was expressed exclusively in the ovary and testis...
September 1, 2012: General and Comparative Endocrinology
C Joy McIntosh, Steve Lawrence, Peter Smith, Jennifer L Juengel, Kenneth P McNatty
The transforming growth factor β (TGFB) superfamily proteins bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9), are essential for mammalian fertility. Recent in vitro evidence suggests that the proregions of mouse BMP15 and GDF9 interact with their mature proteins after secretion. In this study, we have actively immunized mice against these proregions to test the potential in vivo roles on fertility. Mice were immunized with either N- or C-terminus proregion peptides of BMP15 or GDF9, or a full-length GDF9 proregion protein, each conjugated to keyhole limpet hemocyanin (KLH)...
February 2012: Reproduction: the Official Journal of the Society for the Study of Fertility
Xiaohui Li, Swamy K Tripurani, Rebecca James, Stephanie A Pangas
Bidirectional signaling between oocytes and granulosa cells is required for normal folliculogenesis. Oocyte-secreted members of the transforming growth factor beta (TGFB) family, growth differentiation factor 9 (GDF9), and bone morphogenetic protein 15 (BMP15) are well-known mediators of granulosa cell function. Deletion in granulosa cells of Smad4, the common SMAD mediating all canonical TGFB-related protein signals, results in infertility. Reciprocal signaling by granulosa cell-expressed TGFB family ligands, such as activin, to the oocyte during follicle development has been proposed but not tested in vivo using conditional knockout mice...
January 2012: Biology of Reproduction
Michelle Myers, Swamy K Tripurani, Brooke Middlebrook, Aris N Economides, Ernesto Canalis, Stephanie A Pangas
The transforming growth factor beta (TGFB) protein family is renowned for its diverse roles in developmental biology including reproduction. Gremlin is a member of the differential screening-selected gene aberrative in neuroblastoma (DAN)/cerberus family of bone morphogenetic protein (BMP) antagonists. Recent studies on gremlin focus on its involvement in embryonic skeletal, lung, and kidney development. To define the role of gremlin (Grem1) in female reproduction, we analyzed postnatal folliculogenesis using global and conditional knockout (cKO) mice for gremlin...
December 2011: Biology of Reproduction
Wei Wang, Xia Chen, Xinxiu Li, Li Wang, Haiyan Zhang, Yu He, Jingjing Wang, Yongyan Zhao, Baole Zhang, Yinxue Xu
FSH plays a critical role in granulosa cell (GC) proliferation and steroidogenesis through modulation by factors including bone morphogenetic proteins family, which belongs to transforming growth factor β (TGFB) superfamily. TGFBs are the key factors in maintaining cell growth and differentiation in ovaries. However, the interaction of FSH and TGFB on the GCs' proliferation and steroidogenesis remains to be elucidated. In this study, we have investigated the role of SMAD4, a core molecule mediating the intracellular TGFB/SMAD signal transduction pathway, in FSH-mediated proliferation and steroidogenesis of porcine GCs...
May 2011: Reproduction: the Official Journal of the Society for the Study of Fertility
Scott E Lankford, Gregory M Weber
Transforming growth factor-beta (TGFbeta) superfamily members are important paracrine and autocrine regulators of ovarian development and steroidogenesis in mammals and birds, but their reproductive roles in fish are not well understood. The activin system, Tgfb, and bone morphogenetic protein 15 (Bmp15) participate in the regulation of follicle maturation in some fish species. In addition, transcript levels of TGFbeta superfamily members and their inhibitor, bambi (bmp and activin-membrane-bound inhibitor), change in the rainbow trout (Oncorhynchus mykiss) ovary during reproductive development including the transition from vitellogenesis to follicle maturation...
September 15, 2010: General and Comparative Endocrinology
Xiaoyan Gong, Elizabeth A McGee
Follicle-stimulating hormone (FSH) is the major regulator of folliculogenesis, but other factors modulate its action, including members of the transforming growth factor (TGF) beta family. The intersection of signal transduction pathways that integrate the follicular response to FSH remains to be elucidated. Herein, we investigated the role of Smad3, a critical molecule mediating the intracellular TGFbeta family proteins, in follicle development and the expression of FSH receptors. We found that gonadotropin stimulation could not induce normal ovulation in Smad3-deficient mice...
October 2009: Biology of Reproduction
Davina Rosairo, Ileana Kuyznierewicz, Jock Findlay, Ann Drummond
Ovarian follicular growth and differentiation in response to transforming growth factor-beta (TGFB) was investigated using postnatal and immature ovarian models. TGFB ligand and receptor mRNAs were present in the rat ovary 4-12 days after birth and at day 25. In order to assess the impact of TGFB1 on follicle growth and transition from the primordial through to the primary and preantral stages of development, we established organ cultures with 4-day-old rat ovaries. After 10 days in culture with FSH, TGFB1, or a combination of the two, ovarian follicle numbers were counted and an assessment of atresia was undertaken using TUNEL...
December 2008: Reproduction: the Official Journal of the Society for the Study of Fertility
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