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Ilaria Cicalini, Claudia Rossi, Damiana Pieragostino, Luca Agnifili, Leonardo Mastropasqua, Maria di Ioia, Giovanna De Luca, Marco Onofrj, Luca Federici, Piero Del Boccio
Metabolomics based on mass spectrometry represents an innovative approach to characterize multifactorial diseases, such as multiple sclerosis (MuS). To date, the most important biomarker source for MuS diagnosis is the cerebrospinal fluid. However, an important goal for research is to identify new molecules in more easily accessible biological fluids. A very interesting biofluid in MuS is represented by tears, considered as an intermediate fluid between the cerebrospinal fluid and serum. In this work, we developed a merged strategy for the analysis of lipids containing choline by Liquid Chromatography coupled to Tandem Mass Spectrometry (LC-MS/MS), as well as for the targeted analysis of free carnitine, acylcarnitines and aminoacids by direct infusion mass spectrometry...
March 13, 2019: International Journal of Molecular Sciences
Johanna Willer, Karin Jöhrer, Richard Greil, Christian Zidorn, Serhat Sezai Çiçek
In our continuing search for new cytotoxic agents, we assayed extracts, fractions, and pure compounds from damiana ( Turnera diffusa ) against multiple myeloma (NCI-H929, U266, and MM1S) cell lines. After a first liquid-liquid solvent extraction, the ethyl acetate layer of an acetone (70%) crude extract was identified as the most active fraction. Further separation of the active fraction led to the isolation of naringenin ( 1 ), three apigenin coumaroyl glucosides 2 ⁻ 4 , and five flavone aglycones 5 ⁻ 9 ...
February 28, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Narayan D Chaurasiya, Jianping Zhao, Pankaj Pandey, Robert J Doerksen, Ilias Muhammad, Babu L Tekwani
The investigation of the constituents that were isolated from Turnera diffusa (damiana) for their inhibitory activities against recombinant human monoamine oxidases (MAO-A and MAO-B) in vitro identified acacetin 7-methyl ether as a potent selective inhibitor of MAO-B (IC50 = 198 nM). Acacetin 7-methyl ether (also known as 5-hydroxy-4', 7-dimethoxyflavone) is a naturally occurring flavone that is present in many plants and vegetables. Acacetin 7-methyl ether was four-fold less potent as an inhibitor of MAO-B when compared to acacetin (IC50 = 50 nM)...
February 23, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Giovanni Ventura, Ramona Abbattista, Cosima Damiana Calvano, Cristina De Ceglie, Ilario Losito, Francesco Palmisano, Tommaso R I Cataldi
RATIONALE: Oleuropein (Ole) has been claimed to mitigate cisplatin (CP) induced acute injury in kidney and liver of mice. In-vitro reactivity of hydrated CP species with Ole, and Ole metabolite hydroxytyrosol (HT) is of great interest as the preliminary step for gathering in-vivo information on the possible physiological role of the Ole/HT-cis-diammineplatinum (II) (Ole/HT-cis-DAP) conjugate. METHODS: Reversed-phase liquid chromatography coupled to electrospray ionization and mass spectrometry using a linear-ion trap instrument (RPLC-ESI-MS) and tandem mass (MS/MS) measurements, both in positive and negative ion mode, revealed the molecular identity of platinum-based conjugates...
January 23, 2019: Rapid Communications in Mass Spectrometry: RCM
Martino Deidda, Cristina Piras, Giulio Binaghi, Damiana Congia, Alessandro Pani, Alberto Boi, Francesco Sanna, Angelica Rossi, Bruno Loi, Christian Cadeddu Dessalvi, Luigi Atzori, Maurizio Porcu, Giuseppe Mercuro
In this study we investigated whether the metabolomic analysis could identify a specific fingerprint of coronary blood collected during primary PCI in STEMI patients. Fifteen samples was subjected to metabolomic analysis. Subsequently, the study population was divided into two groups according to the peripheral blood neutrophil-to-lymphocyte ratio (NLR), a marker of the systemic inflammatory response. Regression analysis was then applied separately to the two NLR groups. A partial least square (PLS) regression identified the most significant involved metabolites and the PLS-class analysis revealed a significant correlation between the metabolic profile and the total ischemic time only in patients with an NLR > 5...
January 22, 2019: Scientific Reports
Giovanni Ventura, Cosima Damiana Calvano, Ilario Losito, Giuliana Bianco, Raffaella Pascale, Francesco Palmisano, Tommaso R I Cataldi
Several mobile phase additives (i.e., organic acids and their ammonium salts) were used to modulate the chromatographic retention of cyanocobalamin and its cis-diaminemonochloroplatinum(II) conjugate, depending on the specific nature of the stationary phase. Regardless the mobile phase additive, the positively charged cyanocobalamin-cis-diaminemonochloroplatinum(II) conjugate was systematically less retained than cyanocobalamin on a conventional octadecyl-silica column. In contrast, the amide-embedded C18 column exhibited a progressive increase in the conjugate retention time upon changing the mobile phase additive from organic (acetic, formic and trifluoroacetic) acids to ammonium salts, ultimately leading to an inversion of the elution order...
January 9, 2019: Journal of Separation Science
Fritz Keinert, Damiana Lazzaro, Serena Morigi
In this paper we propose a Group-Sparse Representation based method with applications to Face Recognition (GSR-FR). The novel sparse representation variational model includes a non-convex sparsity-inducing penalty and a robust non-convex loss function. The penalty encourages group sparsity by using approximation of the ℓ0-quasinorm, and the loss function is chosen to make the algorithm robust to noise, occlusions and disguises. The solution of the non-trivial non-convex optimization problem is efficiently obtained by a majorization-minimization strategy combined with forward-backward splitting, which in particular reduces the solution to a sequence of easier convex optimization sub-problems...
January 1, 2019: IEEE Transactions on Image Processing: a Publication of the IEEE Signal Processing Society
Francesco Danza, Damiana Ravasi, Nicola Storelli, Samuele Roman, Samuel Lüdin, Matthieu Bueche, Mauro Tonolla
The meromictic Lake Cadagno is characterized by a compact chemocline with high concentrations of anoxygenic phototrophic purple and green sulfur bacteria. However, a complete picture of the bacterial diversity, and in particular of effects of seasonality and compartmentalization is missing. To characterize bacterial communities and elucidate relationships between them and their surrounding environment high-throughput 16S rRNA gene pyrosequencing was conducted. Proteobacteria, Chlorobi, Verrucomicrobia, and Actinobacteria were the dominant groups in Lake Cadagno water column...
2018: PloS One
Fatma M El-Demerdash, Ali B Jebur, Hoda M Nasr, Hanin M Hamid
Oxidative stress and increased production of reactive oxygen species have been implicated in pesticides and heavy metals toxicity. The objective of this study was to investigate the efficacy of Turnera diffusa Willd (damiana) on counteracting fenitrothion (FNT) and/or potassium dichromate (CrVI)-induced testicular toxicity and oxidative injury in rats. FNT and/or CrVI intoxicated animals revealed a significant increase in thiobarbituric acid reactive substances and hydrogen peroxide levels. While, reduced glutathione and protein content, as well as antioxidant enzymes, phosphatases, and aminotransferases activities, were significantly decreased...
December 22, 2018: Environmental Toxicology
Simone Scarlata, Damiana Mancini, Alice Laudisio, Antonelli Incalzi Raffaele
We aimed at testing M-mode ultrasound diaphragmatic thickness reproducibility and its clinical correlates in healthy volunteers. Sixty-six consecutive healthy volunteers were considered eligible, and enrolled in the study. During a single visit, all participants received three M-mode and B-mode ultrasound evaluations of Diaphragmatic Thickness (DT). We then proceeded to calculate the thickness difference and assess the association of ultrasonographic measurements with demographic and anthropometric data. Variables associated through univariate analyses were entered in multivariable models, and Intraclass Correlation Coefficient (ICC) was performed in order to determine intra- and inter-observer reproducibility...
February 2019: Respiratory Physiology & Neurobiology
Mónica García-Peris, M Isabel Jiménez Candel, Yolanda Mañes Jiménez, Macarena Pariente Martí, Damiana González Granda, Fernando Calvo Rigual
INTRODUCTION: The aim of this study is to assess epidemiological, clinical and laboratory characteristics of primary infection by Epstein-Barr virus (EBV) in children without previous diagnosis of any immune disease and its relationship with clinical presentation. PATIENTS AND METHODS: A retrospective study was conducted on all children from 0 to 15 years with IgM against viral capsid of EBV positive or indeterminate during a 22 month period. Epidemiological, clinical and laboratory data were analysed and compared between typical (mononucleosis syndrome) and non-typical clinical symptoms...
October 24, 2018: Anales de Pediatría: Publicación Oficial de la Asociación Española de Pediatría (A.E.P.)
Annalisa Adinolfi, Silvia Zelli, Damiana Leo, Cristiana Carbone, Liudmila Mus, Placido Illiano, Enrico Alleva, Raul R Gainetdinov, Walter Adriani
Dopamine (DA) is a key neurotransmitter of the central nervous system, whose availability is regulated by the dopamine transporter (DAT). Deletion of DAT gene leading to hyperdopaminergia was previously performed on mouse models. This enabled recapitulation of the core symptoms of Attention-Deficit / Hyper-activity Disorder (ADHD), which include hyperactivity, inattention and cognitive impairment. We used recently developed DAT knockout (DAT-KO) rats to carry out further behavioral profiling on this novel model of hyperdopaminergia...
November 22, 2018: Behavioural Brain Research
Alexander Batista-Duharte, Damiana Téllez-Martínez, Cleverton Roberto de Andrade, Deivys Leandro Portuondo, Juliana Aparecida Jellmayer, Marisa Campos Polesi, Iracilda Zeppone Carlos
Little is known about the differences in the CD4+ T-cell response induced by Sporothrix schenckii and Sporothrix brasiliensis, the most virulent species that cause sporotrichosis. Here, the helper (Th) and regulatory T cells (Tregs) responses were evaluated comparatively in a murine model of sporotrichosis on days 7, 21 and 35 after subcutaneous infection with either S. schenckii or S. brasiliensis conidia. The fungal load was measured at the site of infection, as well as in the liver and spleen. The Th1/Th17/Tregs responses were analyzed in the spleen, while the level of IL-2, IL-4, IL-6, TNF-alpha, IFN-ɣ, IL-17A and IL-10 cytokines were measured at the local site of infection on 24 h postinfections and in sera on the indicated days...
December 2018: Fungal Biology
Claudia Rossi, Ilaria Cicalini, Mirco Zucchelli, Maria di Ioia, Marco Onofrj, Luca Federici, Piero Del Boccio, Damiana Pieragostino
Multiple sclerosis (MuS) is an autoimmune disease of the central nervous system characterized by neuroinflammation, neurodegeneration, and degradation of the myelin sheath. Epidemiological studies have shown that the female gender is more susceptible than the male gender to MuS development, with a female-to-male ratio of 2:1. Despite this high onset, women have a better prognosis than men, and the frequency of the relapsing phase decreases during pregnancy, while it increases soon after birth. Therefore, it is interesting to investigate hormonal fluctuations during pregnancy and whether they correlate with metabolic signatures...
November 14, 2018: International Journal of Molecular Sciences
Hugo Luca Abate, Nelson Jessé Rodrigues Dos Santos, Danilo Rodrigues Barros Brito, Jessica Damiana Marinho Valente, Thállitha Samih Wischral Jayme Vieira, João Luis Garcia, Rafael Felipe da Costa Vieira, Odilon Vidotto
Anaplasma marginale and piroplasm species are widespread among Brazilian cattle herds. Both of these tick-borne pathogens hamper livestock production and cause a significant economic impact. Although buffaloes have demonstrated a high level of adaptability, data on tick-borne pathogens are scarcely reported in Brazil. Thus, the aim of this study was to screen water buffaloes from the state of Maranhão for piroplasm and A. marginale occurrence using PCR assays. All samples were negative for A. marginale. One of the 287 (0...
November 8, 2018: Brazilian Journal of Veterinary Parasitology
Sandra Trindade Low, Ana Paula Costa Rodrigues Ferraz, Regiane Maio, Fabiane Valentini Francisqueti, Damiana Tortolero Pierine, Weidylla Natália Silva Borges, Ariel Dandara de Carvalho, Gedalva Pereira de Lima, Ana Lúcia A Ferreira, Klinsmann Carolo Dos Santos, Camila Renata Corrêa
OBJECTIVE: The aim of the present study was to investigate the association of the redox state via malondialdehyde (MDA) as a lipid peroxidation biomarker and hydrophilic antioxidant capacity (HAC) with dietary, anthropometric, demographic, socio-economic and clinical variables as well as the serum concentrations of vitamins in children aged 20-36 months. This cross-sectional study was conducted from May 2013 to May 2014 and included a total of 100 children. METHODS: The variables studied included anthropometric measurements, dietary intake by the Food Frequency Questionnaire (FFQ), socio-demographic features, clinical attributes, serum redox status, and serum vitamin concentrations...
October 18, 2018: Clinics
Damiana Leo, Ilya Sukhanov, Raul R Gainetdinov
No abstract text is available yet for this article.
December 2018: Neural Regeneration Research
Carla R Angelani, Pablo Carabias, Karen M Cruz, José M Delfino, Marilina de Sautu, María V Espelt, Mariela S Ferreira-Gomes, Gabriela E Gómez, Irene C Mangialavori, Malena Manzi, María F Pignataro, Nicolás A Saffioti, Damiana M Salvatierra Fréchou, Javier Santos, Pablo J Schwarzbaum
Metabolic control analysis (MCA) is a promising approach in biochemistry aimed at understanding processes in a quantitative fashion. Here the contribution of enzymes and transporters to the control of a given pathway flux and metabolite concentrations is determined and expressed quantitatively by means of numerical coefficients. Metabolic flux can be influenced by a wide variety of modulators acting on one or more metabolic steps along the pathway. We describe a laboratory exercise to study metabolic regulation of human erythrocytes (RBCs)...
September 2018: Biochemistry and Molecular Biology Education
Francesca Caccuri, Elena Muraro, Annunziata Gloghini, Ombretta Turriziani, Mara Riminucci, Cinzia Giagulli, Katy Mastorci, Damiana Antonia Fae', Simona Fiorentini, Antonino Carbone, Arnaldo Caruso, Riccardo Dolcetti
Despite antiretroviral therapy, HIV+ individuals still have increased risk to develop lymphomas, including marginal zone lymphomas, suggesting that factors other than HIV-related immunosuppression are probably acting as lymphomagenic factors in the HIV setting. The possible pathogenic involvement of HIV p17 protein variants was investigated in a particularly informative case of HIV-related splenic marginal zone lymphoma, who was negative for oncogenic virus infections, thus allowing us to assess the possible direct contribution of these HIV-encoded proteins to lymphomagenesis...
September 27, 2018: Hematological Oncology
Damiana Scuteri, Maria Roberta Garreffa, Stefania Esposito, Giacinto Bagetta, Maria Diana Naturale, Maria Tiziana Corasaniti
During the clinical course of dementia, beside cognitive impairment and memory loss, a very complex challenge is posed by the neuropsychiatric symptoms (NPSs). Accurate evaluation and treatment of pain impacts positively the agitation of demented patients aged ≥ 65 years. To gather information on the utilization of pain killers in demented patients a preliminary survey has been conducted in collaboration with the Calabrian Pharmacovigilance Territorial Service of the health district of Catanzaro (Italy). The study has taken into consideration the prescriptions of acetylcholinesterase inhibitors and memantine during the period ranging from July 2015 to June 2016 and the percentage of patients treated against pain with non steroidal antinflammatory drugs, opioids, and anticonvulsants have been monitored...
September 2018: Neural Regeneration Research
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