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Collagen and Elastin Studies

Tanya Tyagi, May Alarab, Yvonne Leong, Stephen Lye, Oksana Shynlova
This study investigates the effect of local oestrogen therapy (LET) on the expression of proteins participating in collagen/elastin biogenesis and immune markers in vaginal tissues of post-menopausal women with severe pelvic organ prolapse (POP). Vaginal biopsies were collected from the anterior vaginal wall of informed and consented 52 post-menopausal women with severe POP undergoing total hysterectomy. Twenty-nine of the 52 women were treated with LET (in the form of vaginal oestrogen cream or tablet), while the remaining 23 untreated patients served as the controls...
February 17, 2019: Journal of Cellular and Molecular Medicine
João Gabriel Roderjan, Lúcia de Noronha, Marco Augusto Stimamiglio, Alejandro Correa, Amanda Leitolis, Ronaldo Rocha Loures Bueno, Francisco Diniz Affonso da Costa
Tissue-engineered heart valves aim to reproduce the biological properties of natural valves with anatomically correct structure and physiological performance. The closest alternative to creating an ideal heart valve substitute is to use decellularized porcine heart valves, due to their anatomy and availability. However, the immunological barrier and the structural maintenance limit the long-term physiological performance of decellularized porcine heart valves. This study investigated the extracellular matrix (ECM) structure of aortic and pulmonary porcine valves decellularized by a low concentration sodium dodecyl sulfate (SDS)-based method in order to determine the ECM scaffold (ECMS) conditions related to remodeling potential...
February 16, 2019: Xenotransplantation
Andrés Vásquez-Rivera, Harriëtte Oldenhof, Andres Hilfiker, Willem F Wolkers
Decellularized heart valves hold promise for their use as bioscaffolds in cardiovascular surgery. Quality assessment of heart valves after decellularization processing and/or storage is time consuming and destructive. Fourier transform infrared spectroscopy (FTIR) allows rapid non-invasive assessment of biomolecular structures in tissues. In this study, IR-spectra taken from different layers of the pulmonary artery trunk and leaflet tissues of decellularized porcine heart valves were compared with those of pure collagen and elastin, the main protein components in these tissues...
February 5, 2019: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Jadwiga Wańczyk-Baszak, Sławomir Woźniak, Bartosz Milejski, Tomasz Paszkowski
Perimenopausal changes caused by oestrogen deficiency are accompanied by a decrease in the content of collagen and elastin in the tissues, leading to thinning of the epithelium and the resultant disappearance of the superficial layer, which leads to smooth muscle dysfunction as well as connective tissue degradation. This aetiopathogenetic chain results in a set of symptoms experienced by approximately 50% of women in the peri- and postmenopausal period. Symptoms of dryness, burning, dyspareunia and urgency contribute to a significant reduction in the quality of sexual function and general comfort of life due to recurrent infections of the vagina, vulva and urinary tract...
December 2018: Przeglad Menopauzalny, Menopause Review
M Putzu, F Causa, Manuel Parente, Israel González de Torre, J C Rodriguez-Cabello, P A Netti
In the field of tissue engineering the choice of materials is of great importance given the possibility to use biocompatible polymers produced by means of biotechnology. A large number of synthetic and natural materials have been used to this purpose and processed into scaffolds using Electrospinning technique. Among materials that could be used for the fabrication of scaffold and degradable membranes, natural polymers such as collagen, elastin or fibroin offer the possibility to design structures strictly similar to the extracellular matrix (ECM)...
February 2019: Regenerative Biomaterials
Justyna A Niestrawska, Peter Regitnig, Christian Viertler, Tina U Cohnert, Anju R Babu, Gerhard A Holzapfel
Arterial walls can be regarded as composite materials consisting of collagen fibers embedded in an elastic matrix and smooth muscle cells. Remodeling of the structural proteins has been shown to play a significant role in the mechanical behavior of walls during pathogenesis of abdominal aortic aneurysms (AAA). In this study, we systematically studied the change in the microstructure, histology and mechanics to link them to AAA disease progression. We performed biaxial extension tests, second-harmonic generation imaging and histology on 15 samples from the anterior part of AAA walls harvested during open aneurysm surgery...
February 5, 2019: Acta Biomaterialia
Ammu Prhashanna, Phillip A Taylor, Jingya Qin, Kristi L Kiick, Arthi Jayaraman
Elastin-like polypeptides (ELPs) are thermoresponsive biopolymers that undergo an LCST-like phase transition in aqueous solutions. The temperature of this LCST-like transition, Tt  , can be tuned by varying the number of repeat units in the ELP, sequence and composition of the repeat units, the solution conditions, and via conjugation to other biomacromolecules. In this study, we show how and why the choice of guest (X) residue in the VPGXG pentad repeat tunes the Tt of short ELPs, (VPGXG)4 , in the free state and when conjugated to collagen-like peptides (CLPs)...
February 4, 2019: Biomacromolecules
R Camarinho, P V Garcia, H Choi, A S Rodrigues
Volcanogenic air pollution studies and their effects on the respiratory system are still outnumbered by studies regarding the effects of anthropogenic air pollution, representing an unknown risk to human population inhabiting volcanic areas worldwide (either eruptive or non-eruptive areas). This study was carried in the archipelago of the Azores- Portugal, in two areas with active volcanism (Village of Furnas and Village of Ribeira Quente) and a reference site (Rabo de Peixe). The hydrothermal volcanism of Furnas volcanic complex is responsible for the release of 1000 t d-1 of CO2 , H2 S, the radioactive gas - radon, among others...
January 25, 2019: Chemosphere
Shuo Wang, Aziz Tokgoz, Yuan Huang, Yongxue Zhang, Jiaxuan Feng, Priya Sastry, Chang Sun, Nichola Figg, Qingsheng Lu, Michael Pf Sutcliffe, Zhongzhao Teng, Jonathan H Gillard
OBJECTIVE: Mechanical properties of healthy, aneurysmal and atherosclerotic arterial tissues are essential for assessing the risk of lesion development and rupture. Strain energy density function (SEDF) has been widely used to describe these properties, where material constants of the SEDF are traditionally determined using the ordinary least square (OLS) method. However, the material constants derived using OLS are usually dependent on initial guesses. METHODS: To avoid such dependencies, Bayesian inference-based estimation was used to fit experimental stress-stretch curves of 312 tissue strips from 8 normal aortas, 19 aortic aneurysms and 21 carotid atherosclerotic plaques to determine the constants, C_1, D_1 and D_2 of the modified Mooney-Rivlin SEDF...
January 25, 2019: IEEE Transactions on Bio-medical Engineering
Samaneh Ghazanfari, Kyle A Alberti, Qiaobing Xu, Ali Khademhossini
Due to the limited success rate of currently available vascular replacements, tissue engineering has received tremendous attention in recent years. A main challenge in the field of regenerative medicine is creating a mechanically functional tissue with a well-organized extracellular matrix, particularly of collagen and elastin. In this study, the native collagen scaffold derived from decellularized tendon sections, as a scaffold having the potential to be used for vascular tissue engineering applications, was studied...
January 26, 2019: Journal of Biomedical Materials Research. Part A
Rohit Y Dhume, Victor H Barocas
Viscoelasticity plays an important role in the mechanical behavior of biological tissues undergoing dynamic loading. Exploring viscoelastic relaxation spectra of the tissue is essential for predicting its mechanical response. Most load-bearing tissues, however, are also composed of networks of intertwined fibers and filaments of, e.g., collagen, elastin. In this work, we show how non-affine deformations within fiber networks affect the relaxation behavior of the material leading to the emergence of structure-dependent time scales in the relaxation spectra...
January 22, 2019: Acta Biomaterialia
Emanuel Kennedy-Feitosa, Isabella Cattani-Cavalieri, Marina Valente Barroso, Bruna Romana-Souza, Lycia Brito-Gitirana, Samuel Santos Valenca
BACKGROUND: Eucalyptol is a monoterpenoid oil present in many plants, principally the Eucalyptus species, and has been reported to have anti-inflammatory and antioxidative effects. HYPOTHESIS/PURPOSE: Since the potential effect of eucalyptol on mouse lung repair has not yet been studied, and considering that chronic obstructive pulmonary disease (COPD) is the fourth leading cause of death worldwide, the aim of this study was to investigate eucalyptol treatment in emphysematous mice...
August 8, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Ying Rui, Zhang Zhaohui, Song Wenshan, Li Bafang, Hou Hu
The objective of this study was to investigate the effect of Mycosporine-like amino acids (MAAs) extracted from Porphyra tenera skin against UV irradiation-induced photoaging using an ICR mouse model of skin photoaging and to explore the curative effects of the compounds in MAAs. The skin damage and collagenous tissue impairments induced by ultraviolet radiation were observed by histopathological analysis, and the effects of MAAs on protecting against skin damage and maintaining an intact structure of collagenous tissue were studied...
December 19, 2018: Journal of Photochemistry and Photobiology. B, Biology
Jesse L Cox, James M Hammel, Stanley J Radio
The search for an ideal material for cardiac tissue repair has led to utilization of porcine small intestinal submucosa extracellular matrix (CorMatrix). Here, we examine the histologic features of CorMatrix and the associated cellular growth at a variety of time intervals. Tissues with CorMatrix from ten patients (4 male, 6 female) with ages ranging from 2 weeks to 2 years, and implant duration ranging from 1 week to 2 years were included in this study. Samples for analysis were collected at autopsy. Surgical repair sites included great vessel repair (n=9), atrial septum defect (n=1), coronary vessels (n=1), as well as aortic (n=1) and mitral valve (n=2) leaflets...
December 21, 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Fang-da Li, Hao Nie, Cui Tian, Hong-Xia Wang, Bao-Hua Sun, Hua-Liang Ren, Xu Zhang, Peng-Zhi Liao, Duan Liu, Hui-Hua Li, Yue-Hong Zheng
Low-molecular mass protein 7 (LMP7) is a proteolytic subunit of the immunoproteasome that is involved in regulating inflammatory responses. However, the role of LMP7 in the pathogenesis of abdominal aortic aneurysm (AAA) remains unknown. In this study, ApoE knockout (KO) or LMP7/ApoE double KO (dKO) mice were infused with angiotensin II (Ang II, 1000 ng/kg per minute) for up to 28 d. We found that LMP7 expression was significantly upregulated in AAA tissues from ApoE KO mice and human patients. Moreover, Ang II infusion markedly increased the incidence and severity of AAA in ApoE KO mice, which was considerably reduced in LMP7/ApoE dKO mice...
January 14, 2019: Journal of Immunology: Official Journal of the American Association of Immunologists
Tao Wang, Nianguo Dong, Huimin Yan, Sze Yue Wong, Wen Zhao, Kang Xu, Dong Wang, Song Li, Xuefeng Qiu
BACKGROUND: Vascular grafts are widely used as a treatment in coronary artery bypass surgery, hemodialysis, peripheral arterial bypass and congenital heart disease. Various types of synthetic and natural materials were experimented to produce tissue engineering vascular grafts. In this study, we investigated in vivo tissue engineering technology in miniature pigs to prepare decellularized autologous extracellular matrix-based grafts that could be used as vascular grafts for small-diameter vascular bypass surgery...
January 11, 2019: Journal of Translational Medicine
Leila B Mostaco-Guidolin, Michael S Smith, Mark Hewko, Bernie Schattka, Michael G Sowa, Arkady Major, Alex C-T Ko
Structural proteins like collagen and elastin are major constituents of the extracellular matrix (ECM). Extracellular matrix degradation and remodeling in diseases significantly impact the micro-organization of these structural proteins. Therefore, tracking the changes of collagen and elastin fiber's morphological features within ECM impacted by disease progression could provide a valuable insight of pathological processes such as tissue fibrosis and atherosclerosis. Benefitted from its intrinsic high-resolution imaging power and superior biochemical specificity, nonlinear optical microscopy (NLOM) is capable of providing information critical to the understanding of ECM remodeling...
January 10, 2019: Journal of Applied Physiology
S Lessa, J Pontello, R Wanick, E Flores, W Costa, F J Sampaio
Lower blepharoplasty is a challenging aesthetic procedure. Despite advances in clinical and neuroanatomical studies related to orbicularis oculi muscle innervation, no study has examined its histopathological aspects in different lower blepharoplasty procedures. This study aimed to assess changes in the pretarsal muscle complex in patients treated with transcutaneous lower blepharoplasty with orbicularis myotomy versus those treated with transconjunctival blepharoplasty without myotomy. METHODS: A total of 268 patients underwent blepharoplasty performed by a single surgeon: transcutaneous lower blepharoplasty in 112 (41...
January 9, 2019: Aesthetic Plastic Surgery
Mirella D'andrea, Anna Perrotta, Maria Ferrillo, Mariateresa Cantelli, Marianna Donnarumma, Gabriella Fabbrocini
BACKGROUND: The oxidative stress, the UV radiations, the matrix metalloproteinases (MMPs) and the hyaluronidase enzyme play an extremly important role in skin aging processes, leading an increasing production of elastase, collagenase and hyaluronidase that brings to degradation of collagen, elastin and hyaluronic acid respectively. They are responsible to provide strength, elasticity and moisture to the skin. Innovative devices were developed based on the effective ingredients against hyaluronidase and MMP...
February 2019: Giornale Italiano di Dermatologia e Venereologia: Organo Ufficiale, Società Italiana di Dermatologia e Sifilografia
Yang Lei, Gaoyang Guo, Wanyu Jin, Mengwei Liu, Yunbing Wang
BACKGROUND: Glutaraldehyde cross-linked bioprosthetic heart valves might fail due to progressive degradation and calcification. METHODS: In this study, we developed a new BHVs preparation strategy named as "HPA/TRA/FMN" that utilized 3,4-hydroxyphenylpropionic acid (HPA)/tyramine (TRA) conjugated pericardium and riboflavin 5'-monophosphate (FMN) initiated photo-cross-linking method. HPA/TRA-pericardium conjugation would provide extra phenol groups for FMN initiated photo-cross-linking...
January 4, 2019: Xenotransplantation
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