Chen Dong, Yicheng Guo, Zechuan Chen, Teng Li, Juan Ji, Chi Sun, Jing Li, Haixia Cao, Yunfei Xia, Zhonghui Xue, Xixi Gu, Qian Liang, Rui Zhao, Ting Fu, Jiaqiang Ma, Shan Jiang, Chunmei Wu, Qiong Fu, Genkai Guo, Yanfeng Bao, Hua Guo, Junling Yang, Min Xu, Xiaoming Zhang, Zizhang Sheng, Zhifeng Gu
OBJECTIVE: The peripheral B cell compartment is heavily disturbed in systemic lupus erythematosus (SLE), but whether B cells develop aberrantly in the bone marrow (BM) is largely unknown. METHODS: We performed single-cell RNA/B cell receptor (BCR) sequencing and immune profiling of BM B cells and classified SLE patients into two groups: early B cell (Pro-B and Pre-B) normal (EBnor ) and EB defective/low (EBlo ) groups. RESULTS: The SLE-EBlo group exhibited more severe disease activity and proinflammatory status, overaction of type I interferon signaling and metabolic pathways within the B cell compartment, and aberrant BCR repertoires compared with the SLE-EBnor group...
November 10, 2023: Arthritis & Rheumatology