keyword
https://read.qxmd.com/read/38530015/hypomethylation-of-the-lncrna-h19-promoter-accelerates-osteogenic-differentiation-of-vascular-smooth-muscle-cells-by-activating-the-erk1-2-pathways
#21
JOURNAL ARTICLE
Taoxia Wang, Meijuan Cheng, Jingjing Jin, Yaling Bai, Dongxue Zhang, Shenglei Zhang, Jinsheng Xu
OBJECTIVE: Vascular calcification is a common chronic kidney disease complication. This study aimed to investigate the function of long non-coding RNA (LncRNA) H19 in vascular calcification to explore new therapeutic strategies. METHODS: We induced osteogenic differentiation and calcification of vascular smooth muscle cells (VSMCs) using β-glycerophosphate. Then, we detected the LncRNA H19 promoter methylation status and Erk1/2 pathways using methylation-specific polymerase chain reaction and western blotting, respectively...
March 2024: Journal of International Medical Research
https://read.qxmd.com/read/38529489/ehmt2-inactivation-in-pancreatic-epithelial-cells-shapes-the-transcriptional-landscape-and-inflammation-response-of-the-whole-pancreas
#22
Gareth Pollin, Angela J Mathison, Thiago M de Assuncao, Anju Thomas, Lida Zeighami, Ann Salmonson, Hongfei Liu, Guillermo Urrutia, Pallavi Vankayala, Stephen J Pandol, Michael T Zimmermann, Juan Iovanna, Victor X Jin, Raul Urrutia, Gwen Lomberk
The Euchromatic Histone Methyl Transferase Protein 2 (EHMT2), also known as G9a, deposits transcriptionally repressive chromatin marks that play pivotal roles in the maturation and homeostasis of multiple organs. Recently, we have shown that EHMT2 inactivation alters growth and immune gene expression networks, antagonizing KRAS-mediated pancreatic cancer initiation and promotion. Here, we elucidate the essential role of EHMT2 in maintaining a transcriptional landscape that protects organs from inflammation...
March 16, 2024: bioRxiv
https://read.qxmd.com/read/38528715/low-density-lipoprotein-receptor-related-protein-5-6-promotes-endometrial-cancer-progression-and-cancer-cell-immune-escape
#23
JOURNAL ARTICLE
Lifan Shen, Chen Zhang, Genhai Zhu
The study investigated the potential association of the low-density lipoprotein (LDL) genome with endometrial cancer progression based on the Gene Expression Omnibus data set and The Cancer Genome Atlas data set. Differential and weighted gene coexpression network analysis was performed on endometrial cancer transcriptome datasets GSE9750 and GSE106191. The protein-protein interaction network was built using LDL-receptor proteins and the top 50 tumor-associated genes. Low-density lipoprotein-related receptors 5/6 (LRP5/6) in endometrial cancer tissues were correlated with oncogenes, cell cycle-related genes, and immunological checkpoints using Spearman correlation...
April 2024: Journal of Biochemical and Molecular Toxicology
https://read.qxmd.com/read/38528641/low-expression-of-mir-182-caused-by-dna-hypermethylation-accelerates-acute-lymphocyte-leukemia-development-by-targeting-pbx3-and-bcl2-mir-182-promoter-methylation-is-a-predictive-marker-for-hypomethylation-agents%C3%A2-%C3%A2-bcl2-inhibitor-venetoclax
#24
JOURNAL ARTICLE
Danyang Li, Yigang Yuan, Chen Meng, Zihan Lin, Min Zhao, Liuzhi Shi, Min Li, Daijiao Ye, Yue Cai, Xiaofei He, Haige Ye, Shujuan Zhou, Haixia Zhou, Shenmeng Gao
BACKGROUND: miR-182 promoter hypermethylation frequently occurs in various tumors, including acute myeloid leukemia, and leads to low expression of miR-182. However, whether adult acute lymphocyte leukemia (ALL) cells have high miR-182 promoter methylation has not been determined. METHODS: To assess the methylation status of the miR-182 promoter, methylation and unmethylation-specific PCR analysis, bisulfite-sequencing analysis, and MethylTarget™ assays were performed to measure the frequency of methylation at the miR-182 promoter...
March 26, 2024: Clinical Epigenetics
https://read.qxmd.com/read/38528605/coordinated-activation-of-dnmt3a-and-tet2-in-cancer-stem-cell-like-cells-initiates-and-sustains-drug-resistance-in-hepatocellular-carcinoma
#25
JOURNAL ARTICLE
Tao Cheng, Changli Zhou, Sicheng Bian, Kelsey Sobeck, Yahui Liu
BACKGROUND: Resistance to targeted therapies represents a significant hurdle to successfully treating hepatocellular carcinoma (HCC). While epigenetic abnormalities are critical determinants of HCC relapse and therapeutic resistance, the underlying mechanisms are poorly understood. We aimed to address whether and how dysregulated epigenetic regulators have regulatory and functional communications in establishing and maintaining drug resistance. METHODS: HCC-resistant cells were characterized by CCK-8, IncuCyte Live-Cell analysis, flow cytometry and wound-healing assays...
March 25, 2024: Cancer Cell International
https://read.qxmd.com/read/38528591/lncrna-rasal2-as1-promotes-mettl14-mediated-m6a-methylation-in-the-proliferation-and-progression-of-head-and-neck-squamous-cell-carcinoma
#26
JOURNAL ARTICLE
Meiting Rong, Ming Zhang, Feihong Dong, Ke Wu, Bingkun Cai, Jinrui Niu, Le Yang, Zhongyan Li, Hui-Yi Lu
BACKGROUND: Long non-coding RNAs (lncRNAs) are key regulators of the 6-methyladenosine (m6A) epigenetic modification, playing a role in the initiation and progression of tumors. However, the regulatory mechanisms in head and neck squamous cell carcinoma (HNSCC) remain elusive. In this study, we investigated the molecular regulatory mechanisms of the lncRNA RASAL2-AS1 in the occurrence and development of HNSCC tumors. METHODS: A bioinformatics analysis was conducted to analyze the expression level of RASAL2-AS1 in HNSCC and normal tissues...
March 25, 2024: Cancer Cell International
https://read.qxmd.com/read/38527449/plasma-cell-free-adenomatous-polyposis-coli-gene-promoter-methylation-as-a-prognostic-biomarker-for-hepatocellular-carcinoma
#27
JOURNAL ARTICLE
Chih-Yang Hsiao, Chang-Yi Lu, Hung-Ju Su, Kai-Wen Huang
INTRODUCTION: Hepatocellular carcinoma (HCC) is a leading cause of cancer death worldwide. Lack of biomarkers for follow-up after treatment is a clinical challenge. DNA methylation has been proposed to be a potential biomarker in HCC. However, there is still lacking of evidence of its clinical use. This study aims to evaluate the value of using plasma Adenomatous Polyposis Coli promoter methylation level (APC-MET) as a potential biomarker in HCC treatment. METHOD: A total of 96 patients with HCC at BCLC stage B underwent local tumor ablation treatment were prospectively included in this study...
March 25, 2024: Oncology
https://read.qxmd.com/read/38526548/stored-white-tea-ameliorates-dss-induced-ulcerative-colitis-in-mice-by-modulating-the-composition-of-the-gut-microbiota-and-intestinal-metabolites
#28
JOURNAL ARTICLE
Zhiyuan Lin, Weidong Dai, Shanshan Hu, Dan Chen, Han Yan, Liang Zeng, Zhi Lin
Changes in the chemical composition of white tea during storage have been studied extensively; however, whether such chemical changes impact the efficacy of white tea in ameliorating colitis remains unclear. In this study, we compared the effects of new (2021 WP) and 10-year-old (2011 WP) white tea on 3% dextrose sodium sulfate (DSS)-induced ulcerative colitis in mice by gavaging mice with the extracts at 200 mg kg-1 day-1 . Chemical composition analysis showed that the levels of 50 compounds, such as flavanols, dimeric catechins, and amino acids, were significantly lower in the 2011 WP extract than in the 2021 WP extract, whereas the contents of 21 compounds, such as N -ethyl-2-pyrrolidinone-substituted flavan-3-ols, theobromine, and (-)-epigallocatechin-3-(3''- O -methyl) gallate, were significantly higher...
March 25, 2024: Food & Function
https://read.qxmd.com/read/38524273/dna-methylation-as-a-triage-tool-for-cervical-cancer-screening-a-meeting-report
#29
REVIEW
F Ricardo Burdier, Dur-E-Nayab Waheed, Belinda Nedjai, Renske D M Steenbergen, Mario Poljak, Marc Baay, Alex Vorsters, Severien Van Keer
INTRODUCTION: DNA methylation is proposed as a novel biomarker able to monitor molecular events in human papillomavirus (HPV) infection pathophysiology, enabling the distinction between HPV-induced lesions with regression potential from those that may progress to HPV-related cancer. METHODS: This meeting report summarises the presentations and expert discussions during the HPV Prevention and Control Board-focused topic technical meeting on DNA methylation validation in clinician-collected and self-collected samples, novel DNA methylation markers discovery, implementation in cervical cancer screening programs, and their potential in women living with human immunodeficiency virus (HIV)...
May 2024: Preventive Medicine Reports
https://read.qxmd.com/read/38523790/high-paternal-homocysteine-causes-ventricular-septal-defects-in-mouse-offspring
#30
JOURNAL ARTICLE
Lian Liu, Xuan Zhang, Hao-Ran Geng, Ya-Nan Qiao, Yong-Hao Gui, Jian-Yuan Zhao
Maternal hyperhomocysteinemia is widely considered as an independent risk of congenital heart disease (CHD). However, whether high paternal homocysteine causes CHD remains unknown. Here, we showed that increased homocysteine levels of male mice caused decreased sperm count, sperm motility defect and ventricular septal defect of the offspring. Moreover, high levels of paternal homocysteine decrease sperm DNMT3A/3B, accompanied with changes in DNA methylation levels in the promoter regions of CHD-related genes...
April 19, 2024: IScience
https://read.qxmd.com/read/38523537/exploring-the-diagnostic-and-prognostic-predictive-values-of-ferroptosis-related-markers-in-lung-adenocarcinoma
#31
JOURNAL ARTICLE
Guoliang Mao, Wuqin Xu, Muhammad Jamil, Wei Zhang, Nanlin Jiao, Yinhua Liu
BACKGROUND: Lung Adenocarcinoma (LUAD), a common and aggressive form of lung cancer, poses significant treatment challenges due to its low survival rates. AIM: To better understand the role of ferroptosis driver genes in LUAD, this study aimed to explore their diagnostic and prognostic significance, as well as their impact on treatment approaches and tumor immune function in LUAD. METHOD: To accomplish the defined goals, a comprehensive methodology incorporating both in silico and wet lab experiments was employed...
March 20, 2024: Current Pharmaceutical Biotechnology
https://read.qxmd.com/read/38523302/superior-adsorptive-removal-of-anionic-azo-dyes-from-aqueous-solutions-using-sulfonic-acid-group-modified-mil-101-graphene-oxide-composite
#32
JOURNAL ARTICLE
Ji-Min Yang, Bo-Wen Liu, Wei Zhang
It is critical to remove organic contaminants from wastewater released by the printing and dyeing industry for addressing water pollution issue. Therefore, the fabrication of new adsorbents with excellent removal efficiencies is an urgent task. A composite of MIL-101 partially functionalized with -SO3 H (MIL-101-SO3 H) and graphene oxide (GO) was prepared by assembling MIL-101-SO3 H truncated octahedrons on the GO framework. The synthesized MIL-101-SO3 H@GO has a superior adsorption efficiency for anionic azo dyes...
March 24, 2024: Langmuir: the ACS Journal of Surfaces and Colloids
https://read.qxmd.com/read/38523251/nab2-stat6-fusions-and-genome-wide-dna-methylation-profiling-predictors-of-patient-outcomes-in-meningeal-solitary-fibrous-tumors
#33
JOURNAL ARTICLE
Kathryn L Eschbacher, Quynh T Tran, Evgeny A Moskalev, Sarah Jenkins, Karen Fritchie, Robert Stoehr, Alissa Caron, Michael J Link, Paul D Brown, Andrew Guajardo, Daniel J Brat, Ashley Wu, Sandro Santagata, David N Louis, Priscilla K Brastianos, Alexander B Kaplan, Brian Alexander, Sabrina Rossi, Fabio Ferrarese, David R Raleigh, Minh P Nguyen, John Gross, Jose Velazquez Vega, Fausto Rodriguez, Arie Perry, Maria Martinez-Lage, Brent A Orr, Florian Haller, Caterina Giannini
Meningeal solitary fibrous tumors (SFT) are rare and have a high frequency of local recurrence and distant metastasis. In a cohort of 126 patients (57 female, 69 male; mean age at surgery 53.0 years) with pathologically confirmed meningeal SFTs with extended clinical follow-up (median 9.9 years; range 15 days-43 years), we performed extensive molecular characterization including genome-wide DNA methylation profiling (n = 80) and targeted TERT promoter mutation testing (n = 98)...
March 24, 2024: Brain Pathology
https://read.qxmd.com/read/38522151/group-differences-in-oxt-methylation-between-patients-with-major-depressive-disorder-and-healthy-controls-a-pre-registered-replication-study
#34
JOURNAL ARTICLE
Simon Sanwald, Christian Montag, Markus Kiefer
Depression is linked to stress which leaves traces in the epigenetic signature of genes. The oxytocin system is implicated in allostatic processes promoting adaption to environmental stressors. Interactions of the oxytocin system with the environment, e.g., methylation of the gene coding for oxytocin (OXT), are candidates for the investigation of the biological underpinnings of depression. Recently, we found hypomethylation of OXT in patients with Major Depressive Disorder (MDD) compared to healthy controls (HC)...
March 16, 2024: Psychiatry Research
https://read.qxmd.com/read/38522131/pgi1-mediated-vascular-oxidative-pentose-phosphate-pathway-modulates-photosynthesis-via-long-distance-cytokinin-signaling
#35
JOURNAL ARTICLE
Ángela María Sánchez-López, Abdellatif Bahaji, Samuel Gámez-Arcas, Nuria De Diego, Ondřej Vrobel, Petr Tarkowski, Edurne Baroja-Fernández, Francisco José Muñoz, Goizeder Almagro, Jose María Seguí-Simarro, Mercedes Tabernero-Mendoza, Lidia López-Serrano, Rafael J L Morcillo, Javier Pozueta-Romero
In Arabidopsis, the plastidial isoform of phosphoglucose isomerase, PGI1, mediates growth and photosynthesis, likely due to its involvement in the vascular production of cytokinins (CK). To examine this hypothesis, we characterized pgi1-2 knockout plants impaired in PGI1 and pgi1-2 plants specifically expressing PGI1 in root tips and vascular tissues. Moreover, to investigate whether the phenotype of pgi1-2 plants is due to impairments in the plastidial oxidative pentose phosphate pathway (OPPP) or the glycolytic pathway, we characterized pgl3-1 plants with reduced OPPP and pfk4pfk5 knockout plants impaired in plastidial glycolysis...
March 12, 2024: Plant Physiology and Biochemistry: PPB
https://read.qxmd.com/read/38522019/dna-hypomethylation-of-syk-induces-oxidative-stress-and-apoptosis-via-the-pkc%C3%AE-p66shc-signaling-pathway-in-diabetic-kidney-disease
#36
JOURNAL ARTICLE
Rui Zhang, Chunmei Qin, Junlin Zhang, HonghongRen, Yiting Wang, Yucheng Wu, Lijun Zhao, Jiali Wang, Jie Zhang, Fang Liu
Epigenetic alterations, especially DNA methylation, have been shown to play a role in the pathogenesis of diabetes mellitus (DM) and its complications, including diabetic kidney disease (DKD). Spleen tyrosine kinase (Syk) is known to be involved in immune and inflammatory disorders. We, therefore, investigated the possible involvement of Syk promoter methylation in DKD, and the mechanisms underlying this process. Kidney tissues were obtained from renal biopsies of patients with early and advanced DKD. A diabetic mouse model (ApoE-/- DM) was generated from ApoE knockout (ApoE-/- ) mice using a high-fat and high-glucose diet combined with low-dose streptozocin intraperitoneal injection...
March 31, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38521933/a-systematic-review-of-high-impact-cpg-sites-and-regions-for-mgmt-methylation-in-glioblastoma-a-systematic-review-of-mgmt-methylation-in-gbm
#37
JOURNAL ARTICLE
David Gibson, Anh Huan Vo, Hannah Lambing, Prithanjan Bhattacharya, Peggy Tahir, Farid F Chehab, Nicholas Butowski
BACKGROUND: MGMT (O 6 -methylguanine-DNA methyltransferase) promoter methylation is a commonly assessed prognostic marker in glioblastoma (GBM). Epigenetic silencing of the MGMT gene by promoter methylation is associated with greater overall and progression free survival with alkylating agent regimens. To date, there is marked heterogeneity in how MGMT promoter methylation is tested and which CpG sites are interrogated. METHODS: To further elucidate which MGMT promoter CpG sites are of greatest interest, we performed comprehensive searches in PubMed, Web of Science, and Embase and reviewed 2,925 article abstracts...
March 23, 2024: BMC Neurology
https://read.qxmd.com/read/38521405/hypermethylation-leads-to-the-loss-of-hoxa5-resulting-in-jag1-expression-and-notch-signaling-contributing-to-kidney-fibrosis
#38
JOURNAL ARTICLE
Xiao Xiao, Wei Wang, Chunyuan Guo, Jiazhu Wu, Sheng Zhang, Huidong Shi, Sangho Kwon, Jiankang Chen, Zheng Dong
Epigenetic regulations, including DNA methylation, are critical to the development and progression of kidney fibrosis, but the underlying mechanisms remain elusive. Here, we show that fibrosis of the mouse kidney was associated with the induction of DNA methyltransferases and increases in global DNA methylation and was alleviated by the DNA methyltransferase inhibitor 5-Aza-2'-deoxycytidine (5-Aza). Genome wide analysis demonstrated the hypermethylation of 94 genes in mouse unilateral ureteral obstruction kidneys, which was markedly reduced by 5-Aza...
March 21, 2024: Kidney International
https://read.qxmd.com/read/38520591/predominance-of-mgmt-promoter-methylation-among-pakistani-glioblastoma-patients
#39
JOURNAL ARTICLE
Noor Muhammad, Samir Fasih, Bilal Malik, Shahid Hameed, Asif Loya, Muhammad Usman Rashid
BACKGROUND: Glioblastoma multiforme (GBM), the most prevalent subgroup of neuroepithelial tumors, is characterized by dismal overall survival (OS). Several studies have linked O6-methylguanine-DNA-methyltransferase (MGMT) promoter methylation to OS in GBM patients. However, MGMT methylation frequencies vary geographically and across ethnicities, with limited data for South Asian populations, including Pakistan. This study aimed to analyze MGMT promoter methylation in Pakistani GBM patients...
March 23, 2024: Molecular Biology Reports
https://read.qxmd.com/read/38520571/adjuvant-re-irradiation-vs-no-early-re-irradiation-of-resected-recurrent-glioblastoma-pooled-comparative-cohort-analysis-from-two-tertiary-centers
#40
JOURNAL ARTICLE
Christoph Straube, Stephanie E Combs, Denise Bernhardt, Jens Gempt, Bernhard Meyer, Claus Zimmer, Friederike Schmidt-Graf, Peter Vajkoczy, Arne Grün, Felix Ehret, Daniel Zips, David Kaul
BACKGROUND: The optimal management strategy for recurrent glioblastoma (rGBM) remains uncertain, and the impact of re-irradiation (Re-RT) on overall survival (OS) is still a matter of debate. This study included patients who achieved gross total resection (GTR) after a second surgery after recurrence, following the GlioCave criteria. METHODS: Inclusion criteria include being 18 years or older, having histologically confirmed locally recurrent IDHwt or IDH unknown GBM, achieving MRI-proven GTR after the second surgery, having a Karnofsky performance status of at least 60% after the second surgery, having a minimum interval of 6 months between the first radiotherapy and the second surgery, and a maximum of 8 weeks from second surgery to the start of Re-RT...
March 23, 2024: Journal of Neuro-oncology
keyword
keyword
170592
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.