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Seminal plasma

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https://read.qxmd.com/read/30867307/modulation-of-the-ccr5-receptor-ligand-axis-by-seminal-plasma-and-the-utility-of-in-vitro-versus-in-vivo-models
#1
Jennifer A Juno, Kathleen M Wragg, Anne B Kristensen, Wen Shi Lee, Kevin J Selva, Renee M van der Sluis, Anthony D Kelleher, Benjamin R Bavinton, Andrew E Grulich, Sharon R Lewin, Stephen J Kent, Matthew S Parsons
Sexual HIV-1 transmission occurs primarily in the presence of semen. Although data from macaque studies suggests CCR5+ CD4+ T cells are initial targets for HIV-1 infection, the impact of semen on T cell CCR5 expression and ligand production remains inconclusive. To determine if semen modulates the lymphocyte CCR5 receptor/ligand axis, primary human T cell CCR5 expression and natural killer (NK) cell anti-HIV-1 antibody-dependent beta chemokine production was assessed following seminal plasma (SP) exposure. Purified T cells produce sufficient quantities of RANTES to result in a significant decline in CCR5bright T cell frequency following 16 hours of SP exposure (p=0...
March 13, 2019: Journal of Virology
https://read.qxmd.com/read/30865706/identification-and-validation-of-circulating-mirnas-as-endogenous-controls-in-obstructive-sleep-apnea
#2
Fernando Santamaria-Martos, Ivan Benítez, Andrea Zapater, Cristina Girón, Lucía Pinilla, Jose Manuel Fernandez-Real, Ferran Barbé, Francisco Jose Ortega, Manuel Sánchez-de-la-Torre
microRNAs (miRNAs) are non-coding RNAs highly relevant as biomarkers for disease. A seminal study that explored the role of miRNAs in obstructive sleep apnea syndrome (OSA) demonstrated their usefulness in clinical management. Nevertheless, the miRNAs that may act as endogenous controls (ECs) have not yet been established. The identification of ECs would contribute to the standardization of these biomarkers in OSA. The objective of the study is to identify miRNAs that can be used as ECs in OSA. We evaluated 100 patients divided into two different cohorts: a learning cohort of 10 non-OSA and 30 OSA patients, and a validation cohort (20 non-OSA and 40 OSA patients)...
2019: PloS One
https://read.qxmd.com/read/30863906/seminal-plasma-exosomes-promising-biomarkers-for-identification-of-male-and-pseudo-males-in-cynoglossus-semilaevis
#3
Bo Zhang, Na Zhao, Lei Jia, Kang Peng, Jinyuan Che, Kunming Li, Xiaoxu He, Jinsheng Sun, Baolong Bao
In mammals, small RNAs enclosed in exosomes have been identified as appropriate signatures for disease diagnosis. However, there is limited information on exosomes derived from seminal plasma, and few studies have reported analyzed the composition of exosomes and enclosed small RNAs in fish. The half-smooth tongue sole (Cynoglossus semilaevis) is an economically important fish for aquaculture, and it exhibits sexual dimorphism: the female gender show higher growth rates and larger body sizes than males. Standard karyotype analysis and artificial gynogenesis tests have revealed that this species uses heterogametic sex determination (ZW/ZZ), and so-called sex-reversed pseudo-males exist...
March 12, 2019: Marine Biotechnology
https://read.qxmd.com/read/30852746/the-association-between-iron-calcium-and-oxidative-stress-in-seminal-plasma-and-sperm-quality
#4
Oumaima Ammar, Zohra Houas, Meriem Mehdi
The present study aimed to determine the level of iron and calcium in the seminal plasma of men with different fertility potentials and to examine its relationship with oxidative stress. Seventy-nine sub-fertile patients with asthenoteratozoospermia (AT), n 27; teratoleucozoospermia (TL), n 20; teratozoospermia (Terato), n 32; and 29 healthy donors were included. The ability of spermatozoa to produce reactive oxygen species (ROS) was evaluated by using nitroblue tetrazolium (NBT) staining. The lipid peroxidation end product, malondialdehyde (MDA), and the trace element levels (iron and calcium) were measured spectrophotometrically...
March 9, 2019: Environmental Science and Pollution Research International
https://read.qxmd.com/read/30844754/ram-seminal-plasma-and-its-functional-proteomic-assessment
#5
REVIEW
Tamara Leahy, Jessica Paige Rickard, Naomi C Bernecic, Xavier Druart, Simon Paul de Graaf
Ejaculation results in the confluence of epididymal spermatozoa with secretions of the accessory sex glands. This interaction is not a prerequisite for fertilisation success but seminal factors do play a crucial role in prolonging the survival of spermatozoa both in vitro and in vivo by affording protection from handling induced stress and some selective mechanisms of the female reproductive tract. Reproductive biologists have long sought to identify specific factors in seminal plasma that influence sperm function and fertility in these contexts...
March 1, 2019: Reproduction: the Official Journal of the Society for the Study of Fertility
https://read.qxmd.com/read/30815916/lack-of-a-clinically-significant-pharmacokinetic-interaction-between-etravirine-and-raltegravir-using-an-original-approach-based-on-drug-metabolism-protein-binding-and-penetration-in-seminal-fluid-a-pharmacokinetic-substudy-of-the-anrs-163-etral-study
#6
Minh Patrick Lê, Marc-Antoine Valantin, Lambert Assoumou, Cathia Soulie, Soizic Le Mestre, Laurence Weiss, Yazdan Yazdanpanah, Jean-Michel Molina, Olivier Bouchaud, François Raffi, Jacques Reynes, Vincent Calvez, Anne-Geneviève Marcelin, Dominique Costagliola, Christine Katlama, Gilles Peytavin
STUDY OBJECTIVE: The ANRS163-ETRAL study showed that etravirine 200 mg/raltegravir 400 mg twice-daily dual therapy was highly effective in the treatment of human immunodeficiency virus (HIV)-infected patients older than age 45 years, with virologic and therapeutic success rates at week 48 of 99.4% and 94.5%, respectively. The objective of this study was to determine whether a clinically significant pharmacokinetic interaction between etravirine and raltegravir exists by assessing steady-state total and unbound etravirine, raltegravir, and inactive raltegravir-glucuronide concentrations 12 hours after last intake (C12h ) in blood plasma (BP) and seminal plasma (SP)...
February 27, 2019: Pharmacotherapy
https://read.qxmd.com/read/30809771/cadmium-in-seminal-plasma-of-fertile-and-infertile-male-dromedary-camels
#7
Ahmed Ali, Derar R Derar, Essam M Abdel-Elmoniem, Tariq I Almundarij
The objective of this study was to investigate the cadmium (Cd) profile in seminal plasma of male dromedary camel with impotentia generandi (post-coital infertility, IG group, n = 14) in comparison with known fertile males (FERT group, n = 5). Andrological examination was carried out for all animals. Semen was collected with electroejaculation and examined for volume, concentration, motility, viability, and abnormality. Seminal fluid was harvested after centrifugation and analyzed for Cd using atomic absorption spectrophotometer...
February 26, 2019: Biological Trace Element Research
https://read.qxmd.com/read/30807994/seminal-exosomes-induce-interleukin-6-and-interleukin-8-secretion-by-human-endometrial-stromal-cells
#8
Shahrokh Paktinat, Seyed Mahmoud Hashemi, Marefat Ghaffari Novin, Samira Mohammadi-Yeganeh, Saghar Salehpour, Amin Karamian, Hamid Nazarian
OBJECTIVE: Exosomes are extracellular microvesicles that participate in intercellular communication. Seminal plasma (SP) contains very large amounts of exosomes which are deposited in female genital tract after insemination. Although the response of vaginal cells to seminal exosomes (SE) is recently being elucidated, the interaction of uterine cells with SE is still unknown. Here, we aimed to evaluate the effect of SE on cytokine secretion by human endometrial stromal cells (eSC). STUDY DESIGN: Exosomes were isolated from the semen samples of healthy men with proven fertility and characterized using common exosome characterization methods...
February 19, 2019: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://read.qxmd.com/read/30800246/characterization-of-monoclonal-and-polyclonal-antibodies-recognizing-prostate-specific-antigen-implication-for-design-of-a-sandwich-elisa
#9
Sahar Raoofi Mohseni, Forough Golsaz-Shirazi, Mostafa Hosseini, Jalal Khoshnoodi, Tannaz Bahadori, Mohammad Ali Judaki, Mahmood Jeddi-Tehrani, Fazel Shokri
Background: Prostate cancer is the second most common cancer in men. Prostate-Specific Antigen (PSA) is a tumor-associated glycoprotein with enzymatic activity which is secreted by the prostate gland. Following entry to the blood, 70-90% of PSA forms complexes with protease inhibitors and its enzymatic activity is inhibited. The serum level of PSA is increased and the rate of free PSA (fPSA) to total PSA is decreased in prostate cancer patients. Therefore, measurement of PSA and fPSA in serum is very valuable for diagnosis and prognosis of prostate cancer...
January 2019: Avicenna Journal of Medical Biotechnology
https://read.qxmd.com/read/30793927/effect-of-cryopreservation-on-lipid-peroxidation-antioxidant-potential-chromatin-integrity-and-mitochondrial-activity-of-indian-red-jungle-fowl-gallus-gallus-murghi-semen
#10
Muhammad S Ansari, Bushra A Rakha, Shamim Akhter, Elisabeth Blesbois, Julian Santiago-Moreno
AIM: The study elucidates the impact of cryopreservation on lipid peroxidation (LPO), antioxidant potential, DNA integrity, and mitochondrial activity of Indian red jungle fowl sperm. MATERIALS AND METHODS: Semen from eight mature cocks was pooled and diluted at 37°C using a specific medium for red fowl species. The diluted semen samples were immediately refrigerated at 4°C for 2 hours (0.275°C min-1 ). Glycerol was then added to a final concentration of 20%, and the samples were equilibrated for 10 minutes at 5°C before loading into 0...
February 22, 2019: Biopreservation and Biobanking
https://read.qxmd.com/read/30793513/in-vitro-aging-of-boar-spermatozoa-role-of-sperm-proximity-and-seminal-plasma
#11
A-M Luther, D Waberski
BACKGROUND: Knowledge on the effect of seminal plasma on sperm function in extended semen during in vitro storage is lacking. OBJECTIVES: The aim was to examine the interactive role of sperm concentration and seminal plasma concentration on boar sperm function during in vitro aging. MATERIAL AND METHODS: Experiment 1: Twenty-one boar ejaculates were aliquoted with Beltsville Thawing Solution into five semen doses containing between 32.5 and 8...
February 21, 2019: Andrology
https://read.qxmd.com/read/30786164/cellular-origin-of-microrna-371a-3p-in-healthy-males-based-on-systematic-urogenital-tract-tissue-evaluation
#12
W P A Boellaard, A J M Gillis, G J L H van Leenders, H Stoop, T van Agthoven, L C J Dorssers, M Dinkelman-Smit, J L Boormans, L H J Looijenga
BACKGROUND: The microRNA-371a-3p (miR-371a-3p) has been reported to be an informative liquid biopsy (serum and plasma) molecular biomarker for both diagnosis and follow-up of patients with a malignant (testicular) germ cell tumor ((T)GCT). It is expressed in all histological cancer elements, with the exception of mature teratoma. However, normal testis, semen, and serum of males with a disrupted testicular integrity without a TGCT may contain miR-371a-3p levels above threshold, of which the cellular origin is unknown...
February 20, 2019: Andrology
https://read.qxmd.com/read/30785892/metabolomic-profiling-reveals-correlations-between-spermiogram-parameters-and-the-metabolites-present-in-human-spermatozoa-and-seminal-plasma
#13
Kathrin M Engel, Sven Baumann, Ulrike Rolle-Kampczyk, Jürgen Schiller, Martin von Bergen, Sonja Grunewald
In 50% of all infertility cases, the male is subfertile or infertile, however, the underlying mechanisms are often unknown. Even when assisted reproductive procedures such as in vitro fertilization and intracytoplasmic sperm injection are performed, the causes of male factor infertility frequently remain elusive. Since the overall activity of cells is closely linked to their metabolic capacity, we analyzed a panel of 180 metabolites in human sperm and seminal plasma and elucidated their associations with spermiogram parameters...
2019: PloS One
https://read.qxmd.com/read/30779221/deterioration-of-semen-quality-and-sperm-dna-integrity-as-influenced-by-cigarette-smoking-in-fertile-and-infertile-human-male-smokers-a-prospective-study
#14
Parameswari Ranganathan, Kamini Aravind Rao, Sridharan Thalaivarasai Balasundaram
In modernized lifestyle smoking is one of the trendy, psychological, and socioeconomic scenarios of young adolescents mainly in the age of the reproductive stage. Based on a number of cigarettes smoked, age, and duration of the smoke, the study aims to search for the profound effects of smoking and its impact on semen parameters, sperm-DNA integrity, and fragmentation of sperm DNA with cotinine and apoptotic caspase-3 marker in the seminal plasma of fertile and infertile smokers. To determine oxidative damage by 8-hydroxy deoxyguanosine (8-OHdG) from isolated sperm DNA (steps: reactive oxygen species washing by nitro blue tetrazolium (NBT), sperm lysis, salt digestion, ethanol washing, and finally with high-performance liquid chromatography analysis)...
February 18, 2019: Journal of Cellular Biochemistry
https://read.qxmd.com/read/30772660/effect-of-%C3%AE-oryzanol-on-testicular-degeneration-induced-by-scrotal-insulation-in-rams
#15
Eduarda Escobar, Sabrina Lopes, Cristiano Malavolta, Juliana Bernera Ramalho, Daniele Missio, Hirya Fernandes Pinto, Melina Bucco Soares, Fábio Gallas Leivas, Daniela Dos Santos Brum, Francielli Weber Santos Cibin
The present study assessed the effects of daily supplementation with 33 mg/metabolic weight (MW) of γ-oryzanol on testicular degeneration induced by scrotal insulation in rams. Eight animals were divided into two groups: Control (subjected to scrotal insulation without treatment) and Gamma (subjected to scrotal insulation and γ-oryzanol treatment). The rams were subjected to scrotal insulation by covering the scrotum with a thermal bag for 72 h. Animals in the Gamma group received 33 mg/MW oral γ-oryzanol once-daily, beginning 7 days before insulation and continuing during insulation and for 20 days afterward, for a total treatment period of 30 days...
February 7, 2019: Theriogenology
https://read.qxmd.com/read/30772659/major-seminal-plasma-proteome-of-rabbits-and-associations-with-sperm-quality
#16
M J B Bezerra, J M Arruda-Alencar, J A M Martins, A G A Viana, A M Viana Neto, J P A Rêgo, R V Oliveira, M Lobo, A C O Moreira, R A Moreira, A A Moura
The present study was conducted to describe the major seminal plasma proteome of rabbits and potential associations between seminal proteins and semen criteria. Semen samples were collected from 18 New Zealand adult rabbits, and seminal plasma proteins were analyzed by 2-D SDS-PAGE and tandem mass spectrometry. Sperm motility, vigor, concentration, morphology and membrane sperm viability were evaluated. Rabbits ejaculated 364 ± 70 million sperm/ml, with 81 ± 6.1% motile cells, 3.8 ± 0.2 vigor and 66...
February 7, 2019: Theriogenology
https://read.qxmd.com/read/30765224/glycosylation-differentially-modulates-membranolytic-and-chaperone-like-activities-of-pdc-109-the-major-protein-of-bovine-seminal-plasma
#17
Bhanu Pratap Singh, Rajeshwer S Sankhala, Abhishek Asthana, T Ramakrishna, Ch Mohan Rao, Musti J Swamy
The major bovine seminal plasma protein, PDC-109, is a mixture of glycosylated (BSP-A1) and non-glycosylated (BSP-A2) isoforms of a 109-residue long polypeptide. It binds to spermatozoa by specifically recognizing choline phospholipids on the plasma membrane and destabilizes it by penetrating the hydrophobic interior, resulting in lipid efflux, which is necessary for sperm capacitation and successful fertilization. PDC-109 also acts as a molecular chaperone and protects target proteins from denaturation and aggregation induced by various types of stress...
February 11, 2019: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/30763807/progress-in-farm-animal-proteomics-the-contribution-of-combinatorial-peptide-ligand-libraries
#18
Egisto Boschetti, Lorenzo E Hernández-Castellano, Pier Giorgio Righetti
The present review covers proteomics discoveries in farm animals using combinatorial peptide ligand libraries (CPLLs). These libraries enhance the identification of low-abundance proteins by compressing the dynamic range of the protein concentration. This technology can be applied in multiple biological fluids, such as plasma or serum, follicular and cerebrospinal fluids, urine, saliva, tears seminal fluid, milk whey and even lymph. The discovery of low-abundance proteins contributes to the continuous monitoring for animal pathologies, which allows early treatment and disease prevention...
February 11, 2019: Journal of Proteomics
https://read.qxmd.com/read/30763273/new-insights-of-the-role-of-%C3%AE-ngf-in-the-ovulation-mechanism-of-induced-ovulating-species
#19
REVIEW
Marcelo H Ratto, Marco A Berland, Mauricio E Silva, Gregg Adams
The type of stimuli triggering GnRH secretion has been used to classify mammalian species into two categories: spontaneous or induced ovulators. In the former, ovarian steroids produced by a mature follicle elicit the release of GnRH from the hypothalamus, but in the latter, GnRH secretion requires coital stimulation. However, the mechanism responsible for eliciting the preovulatory LH surge in induced ovulators is still not well understood and seems to vary among species. The main goal of this review is to offer new information regarding the mechanism that regulates coitus-induced ovulation...
February 1, 2019: Reproduction: the Official Journal of the Society for the Study of Fertility
https://read.qxmd.com/read/30743098/the-effect-of-l-arginine-and-flaxseed-on-plasma-testosterone-concentration-semen-quality-and-some-testicular-histology-parameters-in-old-broiler-breeder-roosters
#20
Behnam Abbaspour, Seyed Davood Sharifi, Shekofeh Ghazanfari, Abdollah Mohammadi-Sangcheshmeh, Shirin Honarbakhsh
The objective of this study was to investigate the effect of dietary supplementation of l-arginine and flaxseed on testosterone and lipid parameters of serum and semen quality, as well as histological and morphometric parameters of testes in old broiler breeder rooster. Fifty four 52-week-old Ross 308 broiler breeder roosters with similar weights (4900 g ± 210) were used for a 6-week trial period in a 3 × 3 factorial arrangement of dietary treatments that three levels of l-arginine (0.52, 0.68 and 0...
February 1, 2019: Theriogenology
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