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Sperm lipid rafts

Bart Leemans, Tom Arjun Edgar Stout, Catharina De Schauwer, Sonia Heras, Hilde Nelis, Maarten Hoogewijs, Ann Van Soom, Bart M Gadella
In contrast to various other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. In particular, stallion spermatozoa fail to penetrate the zona pellucida, most likely due to incomplete activation of stallion spermatozoa (capacitation) under in vitro conditions. In other mammalian species, specific capacitation triggers have been described; unfortunately, none of these is able to induce full capacitation in stallion spermatozoa. Nevertheless, knowledge of capacitation pathways and their molecular triggers might improve our understanding of capacitation-related events observed in stallion sperm...
February 1, 2019: Reproduction: the Official Journal of the Society for the Study of Fertility
Layla Simón, Abi K Funes, María A Monclús, Regina Colombo, María E Cabrillana, Tania E Saez Lancellotti, Miguel W Fornés
High-fat diet is associated with hypercholesterolemia and seminal alterations in White New Zealand rabbits. We have previously reported disorders in the development of the manchette-acrosome complex during spermiogenesis and decreased testicular efficiency in hypercholesterolemic rabbits. On the other hand, olive oil incorporated into the diet improves cholesterolemia and semen parameters affected in hypercholesterolemic rabbits. In this paper, we report the recovery-with the addition of olive oil to diet-from the sub-cellular mechanisms involved in the shaping of the sperm cell and testicular efficiency altered in hypercholesterolemic rabbits...
2018: PloS One
Karolina Sapoń, Teresa Janas, Aleksander F Sikorski, Tadeusz Janas
Polysialic acid (polySia) forms linear chains which are usually attached to the external surface of the plasma membrane mainly through the Neural Cell Adhesion Molecule (NCAM) protein. It is exposed on neural cells, several types of cancer cells, dendritic cells, and egg and sperm cells. There are several lipid raft-related phenomena in which polySia is involved; however the mechanisms of polySia action as well as determinants of its localization in lipid raft microdomains are still unknown, although the majority of NCAM molecules in the liquid-ordered raft membrane fractions of neural cells appear to be polysialylated...
July 21, 2018: Biochimica et biophysica acta. Biomembranes
Nathaly Cormier, John J McGlone, John Leszyk, Daniel M Hardy
Sperm competence in animal fertilization requires the collective activities of numerous sperm-specific proteins that are typically alloimmunogenic in females. Consequently, sperm membrane alloantigens are potential targets for contraceptives that act by blocking the proteins' functions in gamete interactions. Here we used a targeted proteomics approach to identify the major alloantigens in swine sperm membranes and lipid rafts, and thereby systematically defined the repertoire of these sperm-specific proteins in a single species...
2018: PloS One
Gayathri D Rajamanickam, John P Kastelic, Jacob C Thundathil
Highly dynamic lipid microdomains (rafts) in the sperm plasma membrane contain several signaling proteins that regulate sperm capacitation. Na/K-ATPase isoforms (testis-specific isoform ATP1A4 and ubiquitous isoform ATP1A1) are abundant in bovine sperm plasma membrane. We previously reported that incubation of bovine sperm with ouabain, a specific Na/K-ATPase ligand, induced tyrosine phosphorylation of several sperm proteins during capacitation. The objective of this study was to investigate the roles of lipid rafts and non-rafts in Na/K-ATPase enzyme activity and signaling during bovine sperm capacitation...
November 2017: Molecular Reproduction and Development
Hitomi Watanabe, Rie Takeda, Keiji Hirota, Gen Kondoh
It is well known that mammalian sperm acquires fertilization ability after several maturation processes, particularly within the female reproductive tract. In a previous study, we found that both glycosylphosphatidylinositol (GPI)-anchored protein (GPI-AP) release and lipid raft movement occur during the sperm maturation process. In several genetic studies, release of GPI-AP is a crucial step for sperm fertilization ability in the mouse. Here, we show that lipid raft movement is also fundamental for sperm to be competent for fertilization by comparing the sperm maturation process of two mouse inbred strains, C57BL/6 and BALB/c...
May 2017: Genes to Cells: Devoted to Molecular & Cellular Mechanisms
Jonathan Van Blerkom, Sarah Zimmermann
The oolemma of the second metaphase mouse oocyte has a relatively large domain in the peri-polar body region that is non-permissive for sperm attachment. In this study, certain biochemical components of this non-permissive domain were examined and compared with permissive regions to investigate the molecular basis of an oolemma that becomes functionally polarized during fertilization; an attempt was also made to determine whether similarities exist in the formation of abnormally occurring non-permissive domains in human oocytes...
October 2016: Reproductive Biomedicine Online
Paul L Wood, Kirsten Scoggin, Barry A Ball, Mats H Troedsson, Edward L Squires
Using a nontargeted lipidomics analysis of equine sperm and seminal plasma, we were able to characterize a diverse array of individual lipids including ethanolamine and choline ether lipids and seminolipids essential to membrane raft function. We also detected, for the first time in sperm, the presence of (O-acyl)-ω-hydroxy-fatty acids (OAHFA) with up to 52 carbon chain lengths, which were localized to the head and not the tail of sperm. The only previous identification of OAHFAs has been in meibomian glands and their sebaceous secretions...
September 15, 2016: Theriogenology
R J Aitken, H M Flanagan, H Connaughton, S Whiting, A Hedges, M A Baker
This study reports, for the first time, the significant (p ≤ 0.01) accumulation of homocysteine residues in low density, defective sperm suspensions isolated from patients attending an infertility clinic. This overabundance of homocysteine was not related to a deficiency in folate availability but may have been a reflection of the oxidative stress that characterizes such defective sperm populations. Direct addition of the homocysteine cyclic congener, homocysteine thiolactone, to human spermatozoa resulted in the rapid induction of mitochondrial reactive oxygen species (ROS) generation (p < 0...
March 2016: Andrology
Renske A van Gestel, Jos F Brouwers, Anton Ultee, J Bernd Helms, Bart M Gadella
Lipid rafts are micro-domains of ordered lipids (Lo phase) in biological membranes. The Lo phase of cellular membranes can be isolated from disordered lipids (Ld phase) after treatment with 1 % Triton  X-100 at 4 °C in which the Lo phase forms the detergent-resistant membrane (DRM) fraction. The lipid composition of DRM derived from Madin-Darby canine kidney (MDCK) cells, McArdle cells and porcine sperm is compared with that of the whole cell. Remarkably, the unsaturation and chain length degree of aliphatic chains attached to phospholipids is virtually the same between DRM and whole cells...
January 2016: Cell and Tissue Research
Elizabeth G Bromfield, R John Aitken, Amanda L Anderson, Eileen A McLaughlin, Brett Nixon
STUDY QUESTION: How does oxidative stress impact upon human sperm-egg interaction and in particular the formation of zona pellucida-receptor complexes on the sperm surface? SUMMARY ANSWER: Oxidative stress during human sperm capacitation resulted in the chemical alkylation of the molecular chaperone heat shock protein A2 (HSPA2), a concomitant reduction in surface expression of the zona pellucida-receptor arylsulphatase A (ARSA) and a severe loss of zona pellucida binding ability...
November 2015: Human Reproduction
Alessandra Andrisani, Gabriella Donà, Elena Tibaldi, Anna Maria Brunati, Chiara Sabbadin, Decio Armanini, Gualtiero Alvisi, Salvatore Gizzo, Guido Ambrosini, Eugenio Ragazzi, Luciana Bordin
Astaxanthin (Asta), a photo-protective red pigment of the carotenoid family, is known for its multiple beneficial properties. In this study, the effects of Asta on isolated human sperm were evaluated. Capacitation involves a series of transformations to let sperm acquire the correct features for potential oocyte fertilization, including the generation of a controlled amount of reactive oxygen species (ROS), cholesterol depletion of the sperm outer membrane, and protein tyrosine phosphorylation (Tyr-P) process in the head region...
September 2015: Marine Drugs
Louise Dubois, Karl K Göran Ronquist, Bo Ek, Gunnar Ronquist, Anders Larsson
Prostasomes are exosomes derived from prostate epithelial cells through exocytosis by multivesicular bodies. Prostasomes have a bilayered membrane and readily interact with sperm. The membrane lipid composition is unusual with a high contribution of sphingomyelin at the expense of phosphatidylcholine and saturated and monounsaturated fatty acids are dominant. Lipid rafts are liquid-ordered domains that are more tightly packed than the surrounding nonraft phase of the bilayer. Lipid rafts are proposed to be highly dynamic, submicroscopic assemblies that float freely within the liquid disordered membrane bilayer and some proteins preferentially partition into the ordered raft domains...
November 2015: Molecular & Cellular Proteomics: MCP
Xiao Shi, Ting Wang, Song Quan
Sulfogalactosylglycerolipid (SGG) is the main glycolipid in male mammalian germ cells, which is selectively and highly expressed in mammalian testes and helps form the lipid bilayer of cell membrane. In the process of spermatogenesis, SGG is involved in the meiosis of spermiocytes. Either deficiency or accumulation of SGG will lead to male infertility. SGG homeostasis in the testis is the premise of normal spermatogenesis. In the process of sperm-zona binding, SGG becomes a component of lipid raft and provides a platform for signal transduction...
February 2015: Zhonghua Nan Ke Xue, National Journal of Andrology
Bradley J Stith
This review emphasizes how lipids regulate membrane fusion and the proteins involved in three developmental stages: oocyte maturation to the fertilizable egg, fertilization and during first cleavage. Decades of work show that phosphatidic acid (PA) releases intracellular calcium, and recent work shows that the lipid can activate Src tyrosine kinase or phospholipase C during Xenopus fertilization. Numerous reports are summarized to show three levels of increase in lipid second messengers inositol 1,4,5-trisphosphate and sn 1,2-diacylglycerol (DAG) during the three different developmental stages...
May 15, 2015: Developmental Biology
Alessandra Moscatelli, Assunta Gagliardi, Lilly Maneta-Peyret, Luca Bini, Nadia Stroppa, Elisabetta Onelli, Claudia Landi, Monica Scali, Aurora Irene Idilli, Patrick Moreau
Pollen tubes are the vehicle for sperm cell delivery to the embryo sac during fertilisation of Angiosperms. They provide an intriguing model for unravelling mechanisms of growing to extremes. The asymmetric distribution of lipids and proteins in the pollen tube plasma membrane modulates ion fluxes and actin dynamics and is maintained by a delicate equilibrium between exocytosis and endocytosis. The structural constraints regulating polarised secretion and asymmetric protein distribution on the plasma membrane are mostly unknown...
2015: Biology Open
Estelle Garénaux, Mayumi Kanagawa, Tomoyuki Tsuchiyama, Kazuki Hori, Takeru Kanazawa, Ami Goshima, Mitsuru Chiba, Hiroshi Yasue, Akemi Ikeda, Yoshiki Yamaguchi, Chihiro Sato, Ken Kitajima
Mammalian sperm acquire fertility through a functional maturation process called capacitation, where sperm membrane molecules are drastically remodeled. In this study, we found that a wheat germ agglutinin (WGA)-reactive protein on lipid rafts, named WGA16, is removed from the sperm surface on capacitation. WGA16 is a prostate-derived seminal plasma protein that has never been reported and is deposited on the sperm surface in the male reproductive tract. Based on protein and cDNA sequences for purified WGA16, it is a homologue of human zymogen granule protein 16 (ZG16) belonging to the Jacalin-related lectin (JRL) family in crystal and primary structures...
February 27, 2015: Journal of Biological Chemistry
Hitomi Watanabe, Toru Takeo, Hiromasa Tojo, Kazuhito Sakoh, Thorsten Berger, Naomi Nakagata, Tak W Mak, Gen Kondoh
Mammalian sperm undergo multiple maturation steps after leaving the testis in order to become competent for fertilization, but the molecular mechanisms underlying this process remain unclear. In terms of identifying factors crucial for these processes in vivo, we found that lipocalin 2 (Lcn2), which is known as an innate immune factor inhibiting bacterial and malarial growth, can modulate sperm maturation. Most sperm that migrated to the oviduct of wild-type females underwent lipid raft reorganization and glycosylphosphatidylinositol-anchored protein shedding, which are signatures of sperm maturation, but few did so in Lcn2 null mice...
May 2014: Development
Atsushi Asano, Jacquelyn L Nelson-Harrington, Alexander J Travis
It is intuitive that fertilization-the start of life-involves communication between a sperm cell and an egg. It has been known that to become able to fertilize an egg, a sperm must first communicate with stimuli in the female tract. For example, sterol removal from the plasma membrane is required for sperm to undergo membrane fusion during acrosome exocytosis (AE). However, how membrane lipid changes were transduced into initiation of AE remained unclear. Recently, we found that sperm phospholipase B (PLB) is activated in response to sterol removal and released into the extracellular fluid by proteolytic cleavage...
November 1, 2013: Communicative & Integrative Biology
Ryan C Bates, Colby P Fees, William L Holland, Courtney C Winger, Khulan Batbayar, Rachel Ancar, Todd Bergren, Douglas Petcoff, Bradley J Stith
We report a new step in the fertilization in Xenopus laevis which has been found to involve activation of Src tyrosine kinase to stimulate phospholipase C-γ (PLC-γ) which increases inositol 1,4,5-trisphosphate (IP3) to release intracellular calcium ([Ca](i)). Molecular species analysis and mass measurements suggested that sperm activate phospholipase D (PLD) to elevate phosphatidic acid (PA). We now report that PA mass increased 2.7 fold by 1 min after insemination and inhibition of PA production by two methods inhibited activation of Src and PLCγ, increased [Ca](i) and other fertilization events...
February 1, 2014: Developmental Biology
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