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Fernando Hayashi Sant'Anna, Adriana Ambrosini, Felipe Lhywinskh Guella, Renan Zanini Porto, Luciane Maria Pereira Passaglia
Paenibacillus shenyangensis and Paenibacillus dauci are Gram-stain-positive, rod-shaped and endospore-forming bacteria originally isolated from soil and carrot samples, respectively, in China. Preliminary comparative genomic analysis showed that these bacteria could constitute a single species. Therefore, in this study, their taxonomic statuses were clarified through distinct genomic metrics and phylogenetic analyses. Paenibacillus shenyangensis A9T and P. dauci H9T presented values of average nucleotide identity (ANI) and its derivative metrics (gANI and OrthoANI) ranging from 97...
November 21, 2018: International Journal of Systematic and Evolutionary Microbiology
Claude Koutouan, Valérie Le Clerc, Raymonde Baltenweck, Patricia Claudel, David Halter, Philippe Hugueney, Latifa Hamama, Anita Suel, Sébastien Huet, Marie-Hélène Bouvet Merlet, Mathilde Briard
Alternaria Leaf Blight (ALB), caused by the fungus Alternaria dauci, is the most damaging foliar disease affecting carrots (Daucus carota). In order to identify compounds potentially linked to the resistance to A. dauci, we have used a combination of targeted and non-targeted metabolomics to compare the leaf metabolome of four carrot genotypes with different resistance levels. Targeted analyses were focused on terpene volatiles, while total leaf methanolic extracts were subjected to non-targeted analyses using liquid chromatography couple to high-resolution mass spectrometry...
September 13, 2018: Scientific Reports
Julia Courtial, Latifa Hamama, Jean-Jacques Helesbeux, Mickaël Lecomte, Yann Renaux, Esteban Guichard, Linda Voisine, Claire Yovanopoulos, Bruno Hamon, Laurent Ogé, Pascal Richomme, Mathilde Briard, Tristan Boureau, Séverine Gagné, Pascal Poupard, Romain Berruyer
Qualitative plant resistance mechanisms and pathogen virulence have been extensively studied since the formulation of the gene-for-gene hypothesis. The mechanisms involved in the quantitative traits of aggressiveness and plant partial resistance are less well-known. Nevertheless, they are prevalent in most plant-necrotrophic pathogen interactions, including the Daucus carota - Alternaria dauci interaction. Phytotoxic metabolite production by the pathogen plays a key role in aggressiveness in these interactions...
2018: Frontiers in Plant Science
Long Jin, So-Ra Ko, Chi-Yong Ahn, Hyung-Gwan Lee, Hee-Mock Oh
Three bacterial strains, designated DS48-6-5T, DS48-6-7 and DS48-6-9, were isolated from a sediment sample taken from Daechung Reservoir (Republic of Korea) at a water depth of 48 m. Cells of the strains were Gram-stain-negative, aerobic, rod-shaped and motile with a single polar flagellum. Comparative 16S rRNA gene sequence studies showed that the three isolates had clear affiliation with Betaproteobacteria and the closest relatives were Rhizobacter bergeniae KCTC 32299T, Rhizobacter dauci DSM 11587T and Rhizobacter fulvus KCTC 12591T with 97...
May 2016: International Journal of Systematic and Evolutionary Microbiology
Caixia Gao, Jin Han, Zhenmin Liu, Xiaofen Xu, Feng Hang, Zhengjun Wu
Strain BD3526(T), isolated from raw yak milk collected in Tibet, China, was studied to determine its taxonomic status. The strain was Gram-reaction positive, motile, catalase-positive, aerobic or facultatively anaerobic. The DNA G+C content of the strain was 47.5 mol% and its peptidoglycan contained meso-diaminopimelic acid. The predominant menaquinone was MK-7. The major cellular fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and C16 : 0. Based on 16S rRNA gene sequence analysis, similarities among strain BD3526(T) and its most closely related species, Paenibacillus shenyangensis A9(T), 'Paenibacillus dauci' H9, 'Paenibacillus wulumuqiensis' Y24 and Paenibacillus hunanensis DSM 22170(T) were 99...
March 2016: International Journal of Systematic and Evolutionary Microbiology
Qian Wu, Liying Zhu, Ling Jiang, Xian Xu, Qing Xu, Zhidong Zhang, He Huang
Paenibacillus dauci sp. nov., a new kind of endophytic actinobacteria, is separated from the inner tissues of carrot sample, which forms intimated associations with carrot acting as biological control agents. Here we report a 5.37-Mb assembly of its genome sequence and other useful information, including the coding sequences (CDSs) responsible for biological processes such as antibiotic metabolic process, antimicrobial metabolism, anaerobic regulation and the biosynthesis of vitamin B and polysaccharide. This novel strain can be a potential source of novel lead products for exploitation in the field of pharmaceutical, agriculture and industry...
September 2015: Genomics Data
V Le Clerc, S Marques, A Suel, S Huet, L Hamama, L Voisine, E Auperpin, M Jourdan, L Barrot, R Prieur, M Briard
Combining biparental and multiparental connected population analyses was useful for the identification of 11 QTLs in two new genetic backgrounds of carrot resistance to Alternaria dauci and for breeding recommendations. Leaf blight due to the fungus Alternaria dauci is the major carrot foliar disease worldwide. Some resistance QTLs have been previously identified in one population, but the evaluation of additional genetic backgrounds with higher level of resistance would give opportunities for breeders to combine them by pyramiding...
November 2015: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Piotr Baranowski, Malgorzata Jedryczka, Wojciech Mazurek, Danuta Babula-Skowronska, Anna Siedliska, Joanna Kaczmarek
In this paper, thermal (8-13 µm) and hyperspectral imaging in visible and near infrared (VNIR) and short wavelength infrared (SWIR) ranges were used to elaborate a method of early detection of biotic stresses caused by fungal species belonging to the genus Alternaria that were host (Alternaria alternata, Alternaria brassicae, and Alternaria brassicicola) and non-host (Alternaria dauci) pathogens to oilseed rape (Brassica napus L.). The measurements of disease severity for chosen dates after inoculation were compared to temperature distributions on infected leaves and to averaged reflectance characteristics...
2015: PloS One
Jing Zhu, Wei Wang, Shan-Hui Li, Su-Qin Song, Yu-Qing Xie, Qi-Yong Tang, Ghenijan Osman, Yu-Hu Shi, Zhi-Dong Zhang, Wen-Jun Li
Two Gram-staining-positive, aerobic, motile, endospore-forming, rod-shaped bacteria, designated strains Y24(T) and H9(T) were isolated from cold spring and carrot (Daucus L.) samples, respectively, in Xinjiang Uyghur Autonomous Region, north-western China. The taxonomic positions of the two new isolates were determined by using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences and DNA-DNA hybridizations showed that strains Y24(T) and H9(T) were two different novel species belonging to the genus Paenibacillus, with Paenibacillus hunanensis FeL05(T) as their closest relative...
April 2015: Archives of Microbiology
Chisa Nakano, Naoto Takahashi, Naoto Tanaka, Sanae Okada
A novel, moderately thermophilic, acidophilic, Gram-variable, rod-shaped, endospore-forming bacterium was isolated from a spoiled mixed vegetable and fruit juice product that had the off-flavour of guaiacol. The bacterium, strain 4F(T), grew aerobically at 20-50 °C (optimum 40 °C) and pH 3.0-6.0 (optimum pH 4.0) and produced acid from glycerol, d-galactose and d-glucose. It contained menaquinone-7 (MK-7) as the major isoprenoid quinone and the DNA G+C content was 49.6 mol%. The predominant cellular fatty acids of strain 4F(T) were ω-alicyclic (ω-cyclohexane fatty acids), which are characteristic of the genus Alicyclobacillus...
February 2015: International Journal of Systematic and Evolutionary Microbiology
Linfang Wei, Meiru Si, Mingxiu Long, Lingfang Zhu, Changfu Li, Xihui Shen, Yao Wang, Liang Zhao, Lei Zhang
A yellowish-pigmented bacterium, designated strain PLGR-1(T), was isolated from the root of Bergenia scopulosa collected from Taibai Mountain in Shaanxi Province, north-west China, and was subjected to a taxonomic study by using a polyphasic approach. Cells of strain PLGR-1(T) were Gram-stain-negative, strictly aerobic, rod-shaped, non-spore-forming and motile with a single polar flagellum. Growth occurred at 7-33 °C (optimum, 25-28 °C), at pH 5.0-10.0 (optimum, pH 6.0-7.0) and with 0-0.5 % (w/v) NaCl (optimum, 0 %)...
February 2015: International Journal of Systematic and Evolutionary Microbiology
Mickaël Lecomte, Latifa Hamama, Linda Voisine, Julia Gatto, Jean-Jacques Hélesbeux, Denis Séraphin, Luis M Peña-Rodriguez, Pascal Richomme, Cora Boedo, Claire Yovanopoulos, Melvina Gyomlai, Mathilde Briard, Philippe Simoneau, Pascal Poupard, Romain Berruyer
Although different mechanisms have been proposed in the recent years, plant pathogen partial resistance is still poorly understood. Components of the chemical warfare, including the production of plant defense compounds and plant resistance to pathogen-produced toxins, are likely to play a role. Toxins are indeed recognized as important determinants of pathogenicity in necrotrophic fungi. Partial resistance based on quantitative resistance loci and linked to a pathogen-produced toxin has never been fully described...
2014: PloS One
L Bertier, H Brouwer, A W A M de Cock, D E L Cooke, C H B Olsson, M Höfte
Despite its association with important agricultural crops, Phytophthora clade 8b is a poorly studied group of species. The clade currently consists of three officially described species (Phytophthora porri, P. brassicae and P. primulae) that are host-specific pathogens of leek, cabbages and Primula spp., respectively. However, over the past few decades, several other clade 8b-like Phytophthoras have been found on a variety of different host plants that were all grown at low temperatures in winter seasons. In this study, a collection of 30 of these isolates was subjected to a phylogenetic study using two loci (the rDNA ITS region and the mitochondrial cox1 gene)...
December 2013: Persoonia
Shunsuke Imai, Reishi Yoshida, Yuki Endo, Yukiyo Fukunaga, Atsushi Yamazoe, Daisuke Kasai, Eiji Masai, Masao Fukuda
A rubber-degrading bacterium, the strain NS21, that was isolated from a soil sample in a botanical garden in Japan (Imai et al., 2011) was examined by phenotypic, phylogenetic and chemotaxonomic approaches to determine its taxonomic position. The strain NS21 was motile, possessing a single polar flagellum and a facultatively anaerobic straight rod. Analysis of the 16S rRNA and gyrB gene sequences of NS21 revealed a close relationship to the genus Rhizobacter. The predominant quinone type was Q-8. The G+C content of the NS21 genomic DNA was 70...
2013: Journal of General and Applied Microbiology
Sophie Brun, Hugo Madrid, Bert Gerrits Van Den Ende, Birgitte Andersen, Carine Marinach-Patrice, Dominique Mazier, G Sybren De Hoog
The genus Alternaria includes numerous phytopathogenic species, many of which are economically relevant. Traditionally, identification has been based on morphology, but is often hampered by the tendency of some strains to become sterile in culture and by the existence of species-complexes of morphologically similar taxa. This study aimed to assess if strains of four closely-related plant pathogens, i.e., accurately Alternaria dauci (ten strains), Alternaria porri (six), Alternaria solani (ten), and Alternaria tomatophila (ten) could be identified using multilocus phylogenetic analysis and Matrix-Assisted Laser Desorption Ionisation Time of Flight (MALDI-TOF) profiling of proteins...
January 2013: Fungal Biology
M Lecomte, C Alenda, F Sement, M Berthet, M Briard, L Hamama, P Poupard, R Berruyer
No abstract text is available yet for this article.
2011: Communications in Agricultural and Applied Biological Sciences
Soumaya Benichou, Anita Dongo, Djamel Eddine Henni, Didier Peltier, Philippe Simoneau
Eleven polymorphic microsatellite markers were isolated from the necrotrophic phytopathogenic fungus Alternaria dauci based on enriched genomic libraries. In order to assess allelic variability, the microsatellite loci were analysed in a collection of 43 isolates. The number of detected alleles in 11 loci ranged from two to 24 (mean 10.4). Test of cross-species amplification and sequencing of the resulting amplicons showed that some of these microsatellites could be used in different species such as Alternaria solani, Alternaria bataticola and Alternaria zinniae...
January 2009: Molecular Ecology Resources
Erko Stackebrandt, Susanne Verbarg, Anja Frühling, Hans-Jürgen Busse, Brian J Tindall
16S rRNA gene sequenced-based phylogeny indicates that Rhizobacter dauci ATCC 43778(T) branches within the radiation of Methylibium type strains. A comparative chemotaxonomic study including fatty acid methyl esters, polar lipids and polyamines reveals significant differences that, in combination with the topology of phylogenetic trees, support a dissection of the genus Methylibium. The proposals of this study include the transfer of Methylibium fulvum to the genus Rhizobacter as Rhizobacter fulvus comb. nov...
October 2009: International Journal of Systematic and Evolutionary Microbiology
Valérie Le Clerc, Anna Pawelec, Christelle Birolleau-Touchard, Anita Suel, Mathilde Briard
In most production areas, Alternaria leaf blight (ALB) is recognized as the most common and destructive foliage disease in carrot. To assess the genetic architecture of carrot ALB resistance, two parental coupling maps were developed with similar number of dominant markers (around 70), sizes (around 650 cM), densities (around 9.5 cM), and marker composition. The F(2:3) progenies were evaluated in field and tunnel for two scoring dates. The continuous distribution of the disease severity value indicated that ALB resistance is under polygenic control...
May 2009: TAG. Theoretical and Applied Genetics. Theoretische und Angewandte Genetik
Maria Cristina Lopes, Victor C Martins
A leaf blight of carrot (Daucus carota) caused by Alternaria dauci was found in Alentejo (Ourique, Portugal). Morphological characteristics of the fungus are described.
December 31, 2008: Revista Iberoamericana de Micología
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