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JOURNAL ARTICLE
Sebastián Soto, Rodrigo Adasme, Paulina Vivanco, Paola Figueroa
OBJECTIVE: To evaluate the efficacy of the Start to move protocol compared to conventional treatment in subjects over 15 years of age hospitalized in the ICU on an improvement in functionality, decrease in ICU-acquired weakness (DAUCI), incidence of delirium, days of mechanical ventilation (MV), length of stay in ICU and mortality at 28 days. DESIGN: randomized controlled clinical trial. SETTING: Intensive Care Unit. PARTICIPANTS: Includes adults older than 15 years with invasive mechanical ventilation more than 48h, randomized allocation...
February 23, 2024: Medicina intensiva
#2
JOURNAL ARTICLE
Jiawen Zheng, Yunyu Liao, Yang Li, Dongsheng Li, Yaping Sun, Zijun Xiao
A plant growth hormone indoleacetic acid-producing strain LX3-4T was isolated from a carrot rhizosphere soil sample collected in Shandong Province, China. It is Gram-stain-positive, non-motile, and has irregular short rod-shaped cells. LX3-4T shared high 16S rRNA gene sequence identity with Microbacterium oleivorans DSM 16091T (99.4%), M. testaceum NBRC 12675T (98.6%), M. marinum DSM 24947T (98.5%), M. resistens NBRC 103078T (98.4%), and M. paraoxydans NBRC 103076T (98.3%). Phylogenetic analysis based on the concatenated gene sequences of 16S rRNA gene, housekeeping genes gryB and rpoB also showed the distinction between strain LX3-4T and other Microbacterium species...
February 14, 2024: Archives of Microbiology
#3
JOURNAL ARTICLE
Khalid M Ghoneem, Abdulaziz A Al-Askar, Seham M A El-Gamal, Ehsan M Rashad, Elsherbiny A Elsherbiny, Shafik D Ibrahim, Samy A Marey, WesamEldin I A Saber
Coriandrum sativum L. is a globally significant economic herb with medicinal and aromatic properties. While coriander leaf blight disease was previously confined to India and the USA, this study presents new evidence of its outbreak in Africa and the Middle East caused by Alternaria dauci . Infected leaves display irregular chlorotic to dark brown necrotic lesions along their edges, resulting in leaf discoloration, collapse, and eventual death. The disease also impacts inflorescences and seeds, significantly reducing seed quality...
November 16, 2023: Plants (Basel, Switzerland)
#4
JOURNAL ARTICLE
Katerina Roth, Derrick Tran, Adam Joelsson, Shannon Green, Abigail B Snyder
Some species of Alicyclobacillus spoil beverages by producing guaiacol. Current culture-based methods detect the presence of Alicyclobacillus spp. and a subsequent peroxidase assay determines if the isolate can produce guaiacol. However, these methods are time consuming and can yield false negatives due to differences in growth optima among species. The purpose of this study was to compare a RT-PCR-based method, the GENE-UP® PRO ACB assay, to the IFU Method No. 12 Enumeration and Enrichment methods. Ten species of Alicyclobacillus were detected using the tested RT-PCR assay, while A...
June 7, 2023: Journal of Food Protection
#5
JOURNAL ARTICLE
Claude Emmanuel Koutouan, Valérie Le Clerc, Anita Suel, Latifa Hamama, Patricia Claudel, David Halter, Raymonde Baltenweck, Philippe Hugueney, Jean-François Chich, Sitti Anlati Moussa, Clémentine Champlain, Sébastien Huet, Linda Voisine, Sandra Pelletier, Sandrine Balzergue, Wilfried Chevalier, Emmanuel Geoffriau, Mathilde Briard
Alternaria leaf blight, caused by the fungus Alternaria dauci , is the most damaging foliar disease of carrot. Some carrot genotypes exhibit partial resistance to this pathogen and resistance Quantitative Trait Loci (rQTL) have been identified. Co-localization of metabolic QTL and rQTL identified camphene, α-pinene, α-bisabolene, β-cubebene, caryophyllene, germacrene D and α-humulene as terpenes potentially involved in carrot resistance against ALB. By combining genomic and transcriptomic analyses, we identified, under the co-localization regions, terpene-related genes which are differentially expressed between a resistant and a susceptible carrot genotype...
January 2, 2023: Metabolites
#6
JOURNAL ARTICLE
Xing Liu, Donghang Zhao, Chenggang Ou, Weidong Hao, Yifan Zhang, Yongheng He, Zhiwei Zhao, Feiyun Zhuang
The fungal genus Alternaria , which causes a variety of crop diseases, is widely distributed in the world. Alternaria leaf blight, caused by Alternaria dauci , is one of the most common and destructive diseases in carrot. The infection of A. dauci leads to dramatic decay on both foliage and taproot in severe cases, which results in significant yield losses. In this study, we sequenced and assembled the genome of A. dauci isolate CALB1, which isolated from the major carrot producing areas of China. A total of 65 contigs were assembled, and the estimated genome size was 34...
November 30, 2022: Plant Disease
#7
JOURNAL ARTICLE
Julia Courtial, Jean-Jacques Helesbeux, Hugo Oudart, Sophie Aligon, Muriel Bahut, Bruno Hamon, Guillaume N'Guyen, Sandrine Pigné, Ahmed G Hussain, Claire Pascouau, Nelly Bataillé-Simoneau, Jérôme Collemare, Romain Berruyer, Pascal Poupard
Alternaria dauci is a Dothideomycete fungus, causal agent of carrot leaf blight. As a member of the Alternaria genus, known to produce a lot of secondary metabolite toxins, A. dauci is also supposed to synthetize host specific and non-host specific toxins playing a crucial role in pathogenicity. This study provides the first reviewing of secondary metabolism genetic basis in the Alternaria genus by prediction of 55 different putative core genes. Interestingly, aldaulactone, a phytotoxic benzenediol lactone from A...
May 17, 2022: Scientific Reports
#8
JOURNAL ARTICLE
Lukman Ahamad, Zaki A Siddiqui
Foliar spray of silicon dioxide (SiO2 NPs), zinc oxide (ZnO NPs) and titanium dioxide (TiO2 NPs) nanoparticles were used for the management of Meloidogyne incognita, Alternaria dauci and Rhizoctonia solani disease complex of carrot. Foliar spray of SiO2 NPs/ZnO NPs or TiO2 NPs increased plant growth attributes, chlorophyll and carotenoid of carrot. Foliar spray of 0.10 mg ml-1 SiO2 NPs caused the highest increase in plant growth, chlorophyll and carotenoid content of leaves followed by spray of 0.10 mg ml-1 ZnO NPs, 0...
November 2021: Experimental Parasitology
#9
JOURNAL ARTICLE
P W Crous, D A Cowan, G Maggs-Kölling, N Yilmaz, E Larsson, C Angelini, T E Brandrud, J D W Dearnaley, B Dima, F Dovana, N Fechner, D García, J Gené, R E Halling, J Houbraken, P Leonard, J J Luangsa-Ard, W Noisripoom, A E Rea-Ireland, H Ševčíková, C W Smyth, A Vizzini, J D Adam, G C Adams, A V Alexandrova, A Alizadeh, E Álvarez Duarte, V Andjic, V Antonín, F Arenas, R Assabgui, J Ballarà, A Banwell, A Berraf-Tebbal, V K Bhatt, G Bonito, W Botha, T I Burgess, M Caboň, J Calvert, L C Carvalhais, R Courtecuisse, P Cullington, N Davoodian, C A Decock, R Dimitrov, S Di Piazza, A Drenth, S Dumez, A Eichmeier, J Etayo, I Fernández, J-P Fiard, J Fournier, S Fuentes-Aponte, M A T Ghanbary, G Ghorbani, A Giraldo, A M Glushakova, D E Gouliamova, J Guarro, F Halleen, F Hampe, M Hernández-Restrepo, I Iturrieta-González, M Jeppson, A V Kachalkin, O Karimi, A N Khalid, A Khonsanit, J I Kim, K Kim, M Kiran, I Krisai-Greilhuber, V Kučera, I Kušan, S D Langenhoven, T Lebel, R Lebeuf, K Liimatainen, C Linde, D L Lindner, L Lombard, A E Mahamedi, N Matočec, A Maxwell, T W May, A R McTaggart, M Meijer, A Mešić, A J Mileto, A N Miller, A Molia, S Mongkolsamrit, C Muñoz Cortés, J Muñoz-Mohedano, A Morte, O V Morozova, L Mostert, R Mostowfizadeh-Ghalamfarsa, L G Nagy, A Navarro-Ródenas, L Örstadius, B E Overton, V Papp, R Para, U Peintner, T H G Pham, A Pordel, A Pošta, A Rodríguez, M Romberg, M Sandoval-Denis, K A Seifert, K C Semwal, B J Sewall, R G Shivas, M Slovák, K Smith, M Spetik, C F J Spies, K Syme, K Tasanathai, R G Thorn, Z Tkalčec, M A Tomashevskaya, D Torres-Garcia, Z Ullah, C M Visagie, A Voitk, L M Winton, J Z Groenewald
Novel species of fungi described in this study include those from various countries as follows: Australia , Austroboletus asper on soil, Cylindromonium alloxyli on leaves of Alloxylon pinnatum, Davidhawksworthia quintiniae on leaves of Quintinia sieberi, Exophiala prostantherae on leaves of Prostanthera sp., Lactifluus lactiglaucus on soil, Linteromyces quintiniae (incl. Linteromyces gen. nov.) on leaves of Quintinia sieberi , Lophotrichus medusoides from stem tissue of Citrus garrawayi , Mycena pulchra on soil, Neocalonectria tristaniopsidis (incl...
December 2020: Persoonia
#10
JOURNAL ARTICLE
Alaa Baazeem, Abdulaziz Almanea, Palanisamy Manikandan, Mohammed Alorabi, Ponnuswamy Vijayaraghavan, Ahmed Abdel-Hadi
Microbial natural biocides have attracted much more attention in recent years in order to avoid the unrestricted use of chemical biocides in the environment. The aim of this study is to analyze the antibacterial and antifungal activities of secondary metabolites and growth promoting, nematicidal, and soil enzyme activity mediated by Trichoderma hamatum FB10. The bactericidal and fungicidal activities were performed using cell-free extract. Results revealed that the selected strain exert antibacterial activity against Acidovorax avenae , Erutimacarafavora , and Xanthomonas campestris ...
April 24, 2021: Journal of Fungi (Basel, Switzerland)
#11
JOURNAL ARTICLE
Martha Leyte-Lugo, Pascal Richomme, Pascal Poupard, Luis M Peña-Rodriguez
Alternaria dauci is the causal agent of Alternaria leaf blight (ALB) in carrot ( Daucus carota ) crops around the world. However, to date, A. dauci has received limited attention in its production of phytotoxic metabolites. In this investigation, the bioassay-guided isolation of the extract from liquid cultures of A. dauci resulted in the isolation of two metabolites identified as α -acetylorcinol ( 1 ) and p -hydroxybenzoic acid ( 2 ), based on their spectroscopic data and results from chemical correlation reactions...
September 2, 2020: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
#12
JOURNAL ARTICLE
Sahar Abdelrazek, Philipp Simon, Micaela Colley, Tesfaye Mengiste, Lori Hoagland
Managing pests in carrot production is challenging. Endophytic microbes have been demonstrated to improve the health and productivity of many crops, but factors affecting endophyte dynamics in carrot is still not well understood. The goal of this study was to determine how crop management system and carrot genotype interact to affect the composition and potential of endophytes to mitigate disease caused by Alternaria dauci, an important carrot pathogen. Twenty-eight unique isolates were collected from the taproots of nine diverse genotypes of carrot grown in a long-term trial comparing organic and conventional management...
2020: PloS One
#13
JOURNAL ARTICLE
Raffaella Soleti, Patricia Mallegol, Grégory Hilairet, Mehdi Frifra, Florent Perrin, Cécile Dubois-Laurent, Sébastien Huet, Pascale Pignon, Laetitia Basset, Emmanuel Geoffriau, Ramaroson Andriantsitohaina
Carrots' genotype and growing conditions influence their potential properties to fight against cardiovascular and metabolic diseases. The present study evaluated the influence of carrot genotypes contrasted by root color (Bolero, Presto, Karotan, Deep Purple, Kintoki and Blanche des Vosges) growing under standard, water-restricted, biotic stress ( Alternaria dauci inoculation), and combined stress conditions (water restriction and A.dauci inoculation). The effect of carrots' polyphenol and carotenoid content was assessed on endothelial and smooth muscle cells, hepatocytes, adipocytes and macrophages functions (oxidative stress, apoptosis, proliferation, lipid accumulation and inflammation)...
January 27, 2020: Nutrients
#14
Hosam O Elansary, Diaa O El-Ansary, Fahed A Al-Mana
The authors wish to make the following corrections to their paper [...].
December 31, 2019: Plants (Basel, Switzerland)
#15
JOURNAL ARTICLE
Hosam O Elansary, Diaa O El-Ansary, Fahed A Al-Mana
Fungal infection of horticultural and cereal crops by Alternaria dauci and Rhizoctonia solani represents an important biotic stress that could be alleviated by application of 5-aminolevulinic acid (ALA) to fertile and poor soils. Therefore, in this study, the morphological, physiological, biochemical, and genetic effects of ALA application (eight weekly applications at 3-10 ppm) to A. dauci- and R. solani- infected Salvia rosmarinus (rosemary) in fertile and poor soils were investigated. ALA-treated plants produced the longest and highest number of branches and had higher fresh and dry weights...
December 9, 2019: Plants (Basel, Switzerland)
#16
JOURNAL ARTICLE
Shehzad Abid Khan, Sang Eun Jeong, Hye Yoon Park, Sang-Suk Lee, Che Ok Jeon
A Gram-stain negative, facultative aerobic bacterial strain, designated strain S-16T , was isolated from soil in South Korea. Colonies were white-milkish and cells were non-motile rods with oxidase- and catalase-positive activities. The growth of strain S-16T was observed at 20-40 °C (optimum, 25-30 °C) and pH 5.5-7.0 (optimum, pH 6.5). Ubiquinone-8 was identified as the sole respiratory quinone and C12:0 , C16:0 , C18:0 , C15:1 ω5c and summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c) were identified as the major fatty acids (>5%)...
February 2020: Current Microbiology
#17
JOURNAL ARTICLE
A K Lees, D M Roberts, J Lynott, L Sullivan, J L Brierley
Real-time loop-mediated isothermal amplification (LAMP) assays for the detection of sporangia of the causal pathogen of late blight, Phytophthora infestans , and spores of the main causal pathogen of early blight, Alternaria solani , were developed to facilitate the in-field detection of airborne inoculum to improve disease forecasting. These assays were compared with an existing real-time PCR assay for P. infestans and a newly developed real-time PCR assay for A. solani . Primers were designed for real-time LAMP of P...
December 2019: Plant Disease
#18
JOURNAL ARTICLE
Ya-Han Chen, Mei-Huan Lu, Dong-Sheng Guo, Ying-Yan Zhai, Dan Miao, Jian-Ying Yue, Chen-Hong Yuan, Ming-Min Zhao, De-Rong An
In this study, two phenol compounds, magnolol and honokiol, were extracted from Magnolia officinalis and identified by LC-MS, 1 H- and 13 C-NMR. The magnolol and honokiol were shown to be effective against seven pathogenic fungi, including Alternaria alternata (Fr.) Keissl, Penicillium expansum (Link) Thom, Alternaria dauci f.sp. solani, Fusarium moniliforme J. Sheld, Fusarium oxysporum Schltdl., Valsa mali Miyabe & G. Yamada, and Rhizoctonia solani J.G. Kühn, with growth inhibition of more than 57%. We also investigated the mechanisms underlying the potential antifungal activity of magnolol and honokiol...
June 6, 2019: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
#19
JOURNAL ARTICLE
Fernando Hayashi Sant'Anna, Adriana Ambrosini, Felipe Lhywinskh Guella, Renan Zanini Porto, Luciane Maria Pereira Passaglia
Paenibacillus shenyangensis and Paenibacillus dauci are Gram-stain-positive, rod-shaped and endospore-forming bacteria originally isolated from soil and carrot samples, respectively, in China. Preliminary comparative genomic analysis showed that these bacteria could constitute a single species. Therefore, in this study, their taxonomic statuses were clarified through distinct genomic metrics and phylogenetic analyses. Paenibacillus shenyangensis A9T and P. dauci H9T presented values of average nucleotide identity (ANI) and its derivative metrics (gANI and OrthoANI) ranging from 97...
January 2019: International Journal of Systematic and Evolutionary Microbiology
#20
JOURNAL ARTICLE
Claude Koutouan, Valérie Le Clerc, Raymonde Baltenweck, Patricia Claudel, David Halter, Philippe Hugueney, Latifa Hamama, Anita Suel, Sébastien Huet, Marie-Hélène Bouvet Merlet, Mathilde Briard
Alternaria Leaf Blight (ALB), caused by the fungus Alternaria dauci, is the most damaging foliar disease affecting carrots (Daucus carota). In order to identify compounds potentially linked to the resistance to A. dauci, we have used a combination of targeted and non-targeted metabolomics to compare the leaf metabolome of four carrot genotypes with different resistance levels. Targeted analyses were focused on terpene volatiles, while total leaf methanolic extracts were subjected to non-targeted analyses using liquid chromatography couple to high-resolution mass spectrometry...
September 13, 2018: Scientific Reports
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