Mariola Słowińska, Łukasz Paukszto, Laura Pardyak, Jan P Jastrzębski, Ewa Liszewska, Joanna Wiśniewska, Krzysztof Kozłowski, Jan Jankowski, Barbara Bilińska, Andrzej Ciereszko
In birds, the zona pellucida (ZP) matrix that surrounds the ovulated oocyte-called the inner perivitelline layer-is involved in sperm-zona interaction and successful fertilization. To identify the important genes and proteins connected with the final step of egg development, next-generation sequencing and two-dimensional electrophoresis, combined with mass spectrometry, were used for the analysis of mature oocytes at the F1 developmental stage. A total of 8161 genes and 228 proteins were annotated. Six subfamilies of genes, with codes ZP, ZP1-4 , ZPD , and ZPAX , were identified, with the dominant expression of ZPD ...
September 30, 2021: International Journal of Molecular Sciences