keyword
https://read.qxmd.com/read/35915350/effect-of-in-vitro-maturation-of-human-oocytes-obtained-after-controlled-ovarian-hormonal-stimulation-on-the-expression-of-development-and-zona-pellucida-related-genes-and-their-interactions
#21
JOURNAL ARTICLE
Jure Bedenk, Tadeja Režen, Nina Jančar, Ksenija Geršak, Irma Virant Klun
In an in vitro fertilization program, approximately 10-15% of oocytes obtained after controlled ovarian stimulation are immature, with germinal vesicles (GVs). These oocytes are usually discarded in clinical practice; however, an in vitro maturation (IVM) procedure can be applied to mature them. There are scarce data in the literature on the effect of IVM on the expression of important development- and zona pellucida (ZP)-related genes in human oocytes; therefore, we wanted to determine this. One hundred nine human oocytes were collected from patients enrolled in an intracytoplasmic sperm injection program...
August 1, 2022: Reproductive Sciences
https://read.qxmd.com/read/35641866/molecular-interactions-associated-with-oxidative-stress-mediated-male-infertility-sperm-and-seminal-plasma-proteomics
#22
REVIEW
Manesh Kumar Panner Selvam, Damayanthi Durairajanayagam, Suresh C Sikka
Male factor issues are responsible for 50% of couples infertility. Seminal oxidative stress is one of the major factors that affect the normal physiological aspects of sperm function such as motility and progression, hyperactivation, capacitation, acrosome reaction and zona-pellucida penetration prior to fertilization. In recent times, high-throughput proteomic platforms are used to identify the proteins associated with these aspects of sperm function as associated with oxidative stress. In this review, we have provided a workflow that includes an overview of advanced proteomic techniques and bioinformatic tools used to interpret proteomic results...
2022: Advances in Experimental Medicine and Biology
https://read.qxmd.com/read/35118392/sperm-morphology-and-dna-fragmentation-after-zona-pellucida-selection
#23
JOURNAL ARTICLE
Rumiana Ganeva, Dimitar Parvanov, Denitsa Velikova, Magdalena Vasileva, Kristina Nikolova, Georgi Stamenov
UNLABELLED: Sperm DNA fragmentation (SDF) and sperm morphological defects can negatively affect ART outcomes. Consequently, there is a need for additional semen processing technique that accounts for sperm DNA status and morphology prior to ICSI. The objective was to evaluate the efficacy of an additional zona pellucida adhesion-based sperm selection for obtaining sperm populations with a high percentage of normal morphology and DNA integrity as compared to native semen and routine swim-up preparation...
July 2021: Reproduction & fertility
https://read.qxmd.com/read/35054946/identification-of-sperm-binding-sites-in-the-n-terminal-domain-of-bovine-egg-coat-glycoprotein-zp4
#24
JOURNAL ARTICLE
Kamila Dilimulati, Misaki Orita, Yoshiki Yonahara, Fabiana Lica Imai, Naoto Yonezawa
The species-selective interaction between sperm and egg at the beginning of mammalian fertilisation is partly mediated by a transparent envelope called the zona pellucida (ZP). The ZP is composed of three or four glycoproteins (ZP1-ZP4). The functions of the three proteins present in mice (ZP1-ZP3) have been extensively studied. However, the biological role of ZP4, which was found in all other mammals studied so far, has remained largely unknown. Previously, by developing a solid support assay system, we showed that ZP4 exhibits sperm-binding activity in bovines and the N-terminal domain of bovine ZP4 (bZP4 ZP-N1 domain) is a sperm-binding region...
January 11, 2022: International Journal of Molecular Sciences
https://read.qxmd.com/read/34983666/targeted-deletion-of-liver-expressed-choriogenin-l-results-in-the-production-of-soft-eggs-and-infertility-in-medaka-oryzias-latipes
#25
JOURNAL ARTICLE
Kenji Murata, Masato Kinoshita
Egg envelopes (chorions) in medaka, Oryzias latipes, are composed of three major glycoproteins: ZI-1, - 2, and - 3. These gene-encoded chorion glycoproteins are expressed in the liver and/or ovarian oocytes of sexually mature female fish. In medaka, the glycoproteins produced in the female liver are induced by estrogen as Choriogenin (Chg.) H and Chg. H minor (m), which correspond to the zona pellucida (ZP) B (ZPB) protein in mammals, and Chg. L, which corresponds to ZPC in mammals. Chg. H, Chg. Hm, and Chg...
January 4, 2022: Zoological Letters
https://read.qxmd.com/read/34845642/paternal-factors-in-recurrent-pregnancy-loss-an-insight-through-analysis-of-non-synonymous-single-nucleotide-polymorphism-in-human-testis-specific-chaperone-hspa2-gene
#26
JOURNAL ARTICLE
Gayatri Mohanty, Soumya Ranjan Jena, Sujata Kar, Luna Samanta
Heat shock protein A2 (HSPA2) is a testis-specific molecular chaperone of the 70 kDa heat shock protein (HSP70) family and reported to play a key role in spermatogenesis as well as in the remodelling of the sperm surface during capacitation. It is established that mice lacking HSPA2 gene are infertile and spermatozoa that fail to interact with the zona pellucida of the oocyte consistently lack HSPA2 protein expression. However, its role in post fertilization events is not fully understood. Owing to the importance of HSPA2 in male reproduction, the present study is undertaken to reveal the association between genetic mutation and phenotypic variation in recurrent pregnancy loss (RPL) patients through an in silico prediction analysis...
November 30, 2021: Environmental Science and Pollution Research International
https://read.qxmd.com/read/34670878/silkworm-recombinant-bovine-zona-pellucida-protein-4-bzp4-as-a-potential-female-immunocontraceptive-antigen-impaired-sperm-oocyte-interaction-and-ovarian-dysfunction
#27
JOURNAL ARTICLE
Ken-Ichiro Tatematsu, Mitsumi Ikeda, Yoshihiro Wakabayashi, Takashi Yamamura, Kazuhiro Kikuchi, Junko Noguchi, Hideki Sezutsu
Porcine zona pellucida proteins (ZPs) have been utilized as female immunocontraceptive antigens. The purpose of this study was to explore the potential use of silkworm recombinant bovine ZP4 as an alternative. When the protein was injected with monophosphoryl lipid A (MPL) - an immuno-stimulative agent - into two female goats, marked elevation of the anti-ZP4 titer was detected. Application of the purified specific IgG to a porcine in vitro fertilization system reduced the sperm penetration rate. In one goat, the cyclic profile of serum progesterone disappeared as the anti-ZP4 titer increased...
December 14, 2021: Journal of Reproduction and Development
https://read.qxmd.com/read/34638931/transcriptome-and-proteome-analysis-revealed-key-pathways-regulating-final-stage-of-oocyte-maturation-of-the-turkey-meleagris-gallopavo
#28
JOURNAL ARTICLE
Mariola Słowińska, Łukasz Paukszto, Laura Pardyak, Jan P Jastrzębski, Ewa Liszewska, Joanna Wiśniewska, Krzysztof Kozłowski, Jan Jankowski, Barbara Bilińska, Andrzej Ciereszko
In birds, the zona pellucida (ZP) matrix that surrounds the ovulated oocyte-called the inner perivitelline layer-is involved in sperm-zona interaction and successful fertilization. To identify the important genes and proteins connected with the final step of egg development, next-generation sequencing and two-dimensional electrophoresis, combined with mass spectrometry, were used for the analysis of mature oocytes at the F1 developmental stage. A total of 8161 genes and 228 proteins were annotated. Six subfamilies of genes, with codes ZP, ZP1-4 , ZPD , and ZPAX , were identified, with the dominant expression of ZPD ...
September 30, 2021: International Journal of Molecular Sciences
https://read.qxmd.com/read/34556579/the-conserved-fertility-factor-spaca4-bouncer-has-divergent-modes-of-action-in-vertebrate-fertilization
#29
JOURNAL ARTICLE
Yoshitaka Fujihara, Sarah Herberg, Andreas Blaha, Karin Panser, Kiyonori Kobayashi, Tamara Larasati, Maria Novatchkova, Hans-Christian Theussl, Olga Olszanska, Masahito Ikawa, Andrea Pauli
Fertilization is the fundamental process that initiates the development of a new individual in all sexually reproducing species. Despite its importance, our understanding of the molecular players that govern mammalian sperm-egg interaction is incomplete, partly because many of the essential factors found in nonmammalian species do not have obvious mammalian homologs. We have recently identified the lymphocyte antigen-6 (Ly6)/urokinase-type plasminogen activator receptor (uPAR) protein Bouncer as an essential fertilization factor in zebrafish [S...
September 28, 2021: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/34556396/a-stallion-spermatozoon-s-journey-through-the-mare-s-genital-tract-in-vivo-and-in-vitro-aspects-of-sperm-capacitation
#30
JOURNAL ARTICLE
Paula Maitan, Elizabeth G Bromfield, Tom A E Stout, Bart M Gadella, Bart Leemans
Conventional in vitro fertilization is not efficacious when working with equine gametes. Although stallion spermatozoa bind to the zona pellucida in vitro, these gametes fail to initiate the acrosome reaction in the vicinity of the oocyte and cannot, therefore, penetrate into the perivitelline space. Failure of sperm penetration most likely relates to the absence of optimized in vitro fertilization media containing molecules essential to support stallion sperm capacitation. In vivo, the female reproductive tract, especially the oviductal lumen, provides an environmental milieu that appropriately regulates interactions between the gametes and promotes fertilization...
September 14, 2021: Animal Reproduction Science
https://read.qxmd.com/read/34459848/the-cell-biology-of-fertilization-gamete-attachment-and-fusion
#31
REVIEW
Karen K Siu, Vitor Hugo B Serrão, Ahmed Ziyyat, Jeffrey E Lee
Fertilization is defined as the union of two gametes. During fertilization, sperm and egg fuse to form a diploid zygote to initiate prenatal development. In mammals, fertilization involves multiple ordered steps, including the acrosome reaction, zona pellucida penetration, sperm-egg attachment, and membrane fusion. Given the success of in vitro fertilization, one would think that the mechanisms of fertilization are understood; however, the precise details for many of the steps in fertilization remain a mystery...
October 4, 2021: Journal of Cell Biology
https://read.qxmd.com/read/34422946/the-proteome-of-equine-oviductal-fluid-varies-before-and-after-ovulation-a-comparative-study
#32
JOURNAL ARTICLE
Pablo Fernández-Hernández, Federica Marinaro, María Jesús Sánchez-Calabuig, Luis Jesús García-Marín, María Julia Bragado, Lauro González-Fernández, Beatriz Macías-García
Equine fertilization cannot be performed in the laboratory as equine spermatozoa do not cross the oocyte's zona pellucida in vitro . Hence, a more profound study of equine oviductal fluid (OF) composition at the pre-ovulatory and post-ovulatory stages could help in understanding what components are required to achieve fertilization in horses. Our work aimed to elucidate the proteomic composition of equine OF at both stages. To do this, OF was obtained postmortem from oviducts of slaughtered mares ipsilateral to a pre-ovulatory follicle ( n = 4) or a recent ovulation ( n = 4); the samples were kept at -80°C until analysis...
2021: Frontiers in Veterinary Science
https://read.qxmd.com/read/34354992/identification-of-sialyl-lewis-x-interacting-protein-on-human-spermatozoa
#33
JOURNAL ARTICLE
Ying Wang, Weie Zhao, Si Mei, Panyu Chen, Tsz-Ying Leung, Cheuk-Lun Lee, William S B Yeung, Jian-Ping Ou, Xiaoyan Liang, Philip C N Chiu
Capacitated spermatozoa initiate fertilization by binding to the zona pellucida (ZP). Defective spermatozoa-ZP binding causes infertility. The sialyl-Lewis(x) (SLeX) sequence is the most abundant terminal sequence on the glycans of human ZP glycoproteins involving in spermatozoa-ZP binding. This study aimed to identify and characterize the SLeX-binding proteins on human spermatozoa. By using affinity chromatography followed by mass spectrometric analysis, chromosome 1 open reading frame 56 (C1orf56) was identified to be a SLeX-binding protein of capacitated spermatozoa...
2021: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/34242308/sperm-binding-regions-on-bovine-egg-zona-pellucida-glycoprotein-zp4-studied-in-a-solid-supported-form-on-plastic-plate
#34
JOURNAL ARTICLE
Kamila Dilimulati, Misaki Orita, Ganbat Undram, Naoto Yonezawa
The zona pellucida (ZP) is a transparent envelope that surrounds the mammalian oocyte and mediates species-selective sperm-oocyte interactions. The bovine ZP consists of the glycoproteins ZP2, ZP3, and ZP4. Sperm-binding mechanisms of the bovine ZP are not yet fully elucidated. In a previous report, we established the expression system of bovine ZP glycoproteins using Sf9 insect cells and found that the ZP3/ZP4 heterocomplex inhibits the binding of sperm to the ZP in a competitive inhibition assay, while ZP2, ZP3, ZP4, the ZP2/ZP3 complex, and the ZP2/ZP4 complex do not exhibit this activity...
2021: PloS One
https://read.qxmd.com/read/34172998/loss-of-spaca1-function-causes-autosomal-recessive-globozoospermia-by-damaging-the-acrosome-acroplaxome-complex
#35
JOURNAL ARTICLE
Pingping Chen, Hexige Saiyin, Ruona Shi, Bin Liu, Xu Han, Yuping Gao, Xiantao Ye, Xiaofei Zhang, Yu Sun
STUDY QUESTION: Is the sperm acrosome membrane-associated protein 1 (SPACA1) gene critical to human globozoospermia? SUMMARY ANSWER: The biallelic loss-of-function (variant of SPACA1) causes globozoospermia as a result of acrosome-acroplaxome complex damage. WHAT IS KNOWN ALREADY: SPACA1 expression decreases in patients with globozoospermia. Spaca1 gene-disrupted mice have abnormally shaped sperm heads that resemble those of human globozoospermia...
August 18, 2021: Human Reproduction
https://read.qxmd.com/read/33783058/the-protein-phosphatase-with-ef-hand-domain-1-is-a-calmodulin-binding-protein-that-interacts-with-proteins-involved-in-sperm-capacitation-binding-to-the-zona-pellucida-and-motility
#36
JOURNAL ARTICLE
Camille Lavoie-Ouellet, Marie-Ève Clark, Juliana Ruiz, Andrée-Anne Saindon, Pierre Leclerc
Spermatozoa are highly specialized cells whose fertilizing and motility functions highly depend on intracellular Ca2+ -mediated events and protein posttranslational modifications like phosphorylation. Our group previously identified PPEF1, the Ser/Thr phosphatase with EF-hand domain 1, among calmodulin-affinity pulled down sperm proteins. As the mammalian ortholog of the Drosophila phosphatase rdgC that dephosphorylates rhodopsin, PPEF1 has been studied mostly in the retina. The presence and importance of this Ca2+ /calmodulin-binding protein phosphatase has not been studied in sperm or testicular functions despite its high expression level...
April 2021: Molecular Reproduction and Development
https://read.qxmd.com/read/33779727/limited-proteolysis-by-acrosin-affects-sperm-binding-and-mechanical-resilience-of-the-mouse-zona-pellucida
#37
JOURNAL ARTICLE
Michael Kuske, Julia Floehr, Irene Yiallouros, Thomas Michna, Willi Jahnen-Dechent, Stefan Tenzer, Walter Stöcker, Hagen Körschgen
The encounter of oocyte and sperm is the key event initiating embryonic development in mammals. Crucial functions of this existential interaction are determined by proteolytic enzymes, such as acrosin, carried in the sperm head acrosome, and ovastacin, stored in the oocyte cortical granules. Ovastacin is released upon fertilisation to cleave the zona pellucida, a glycoprotein matrix surrounding the oocyte. This limited proteolysis hardens the oocyte envelope, and thereby provides a definitive block against polyspermy and protects the developing embryo...
March 24, 2021: Molecular Human Reproduction
https://read.qxmd.com/read/33685549/porcine-oocyte-preincubation-in-oviductal-fluid-flush-before-in-vitro-fertilization-in-the-presence-of-oviductal-epithelial-cells-improves-monospermic-zygote-production
#38
JOURNAL ARTICLE
Ribrio Ivan Tavares Pereira Batista, Lucia N Moro, Emilie Corbin, Carmen Alminana, Joanna Maria Gonçalves Souza-Fabjan, Vicente José de Figueirêdo Freitas, Pascal Mermillod
The present study was designed to evaluate the effect of the combination of oviduct fluid flush (OFF) and oviduct epithelial cells (OEC) in modulating the incidence of polyspermy in pigs. Therefore, for in vitro fertilization (IVF), oocyte and sperm were co-cultured in Tris-buffered medium (TBM) either supplemented with 10% OFF (OFFD group), or in the presence of a bovine OEC monolayer (OEC group), or the oocytes were exposed to OFF for 30 min before IVF (OFFB group), or in the presence of an OEC monolayer (OFFB + OEC group)...
October 2021: Zygote: the Biology of Gametes and Early Embryos
https://read.qxmd.com/read/33681199/human-zona-pellucida-glycoproteins-binding-characteristics-with-human-spermatozoa-and-induction-of-acrosome-reaction
#39
REVIEW
Satish Kumar Gupta
Human zona pellucida (ZP) matrix is composed of four glycoproteins designated as ZP glycoprotein -1 (ZP1), -2 (ZP2), -3 (ZP3), and -4 (ZP4). Mutations in the genes encoding human ZP glycoproteins are one of the causative factors leading to abnormal ZP matrix and infertility in women. Relevance of the human ZP glycoproteins in 'sperm-oocyte' binding has been delineated by using either transgenic animal models expressing human zona proteins or purified native/recombinant human zona proteins. Studies based on the purified native/recombinant human zona proteins revealed that ZP1, ZP3, and ZP4 primarily bind to the capacitated acrosome-intact human spermatozoa whereas ZP2 binds to acrosome-reacted spermatozoa...
2021: Frontiers in Cell and Developmental Biology
https://read.qxmd.com/read/33498624/arylsulfatase-a-remodeling-during-human-sperm-in-vitro-capacitation-using-field-emission-scanning-electron-microscopy-fe-sem
#40
JOURNAL ARTICLE
María José Gómez-Torres, Natalia Huerta-Retamal, Laura Robles-Gómez, Paula Sáez-Espinosa, Jon Aizpurua, Manuel Avilés, Alejandro Romero
Capacitation drives sperm biophysical and biochemical changes for sperm-oocyte interactions. It is a well-known fact that the molecular complex arylsulfatase A (ARSA), hyaluronidase sperm adhesion molecule 1 (SPAM1), and heat shock protein 2 (HSPA2) plays a significant role in sperm-zona pellucida (ZP) binding. However, the time-dependent capacitation effects on the sperm surface ARSA presence and specific topographic distributions remain to be elucidated. Here, we quantified the ARSA density and specific membrane domain locations before (US) and after in vitro capacitation (one and four hours; CS1-CS4) in human sperm using high-resolution field emission scanning electron microscopy (FE-SEM) and immunogold labeling...
January 23, 2021: Cells
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