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Sperm acrosome reaction

Shuly Lim, Michelina Kierzek, Anne E O'Connor, Christoph Brenker, D Jo Merriner, Hidenobu Okuda, Marianna Volpert, Avinash Gaikwad, Deborah Bianco, David Potter, Ranganathan Prabhakar, Timo Strünker, Moira K O'Bryan
The Cysteine-RIch Secretory Proteins (CRISPs) are a group of proteins which show a pronounced expression biased to the male reproductive tract. While sperm encounter CRISPs at virtually all phases of sperm development and maturation, CRISP2 is the sole CRISP produced during spermatogenesis, wherein it is incorporated into the developing sperm head and tail. Within this study we test the necessity for CRISP2 in male fertility using Crisp2 loss-of-function mouse models. In doing so, we reveal a role for CRISP2 in establishing the ability of sperm to undergo the acrosome reaction, and in establishing a normal flagellum wave form...
February 13, 2019: Endocrinology
Elina Tsirulnikov, Yair Huta, Haim Breitbart
In order to fertilize the egg, the spermatozoon should undergo a process called acrosomal exocytosis or acrosome reaction (AR), a process which can take place only after a series of biochemical changes collectively called capacitation occur in the female reproductive tract. We present here for the first time the involvement of protein-kinase A (PKA) and phosphatidyl-inositol-3-kinase (PI3K) in protecting sperm from undergoing spontaneous AR (sAR) which decreases fertilization rate. Previously we showed that Calmodulin-kinase II (CaMKII) and phospholipase-D (PLD) prevent the occurrence of sAR in two distinct pathways by enhancing actin polymerization...
February 1, 2019: Theriogenology
Wauranittha Timklay, Sirilug Magerd, Chihiro Sato, Monsicha Somrit, Atthaboon Watthammawut, Thanyaporn Senarai, Wattana Weerachatyanukul, Ken Kitajima, Somluk Asuvapongpatana
Glycoconjugates in egg extracellular matrices are known to serve several functions in reproductive processes. Here, the presence of N-linked mannose (Man) glycoconjugates on shrimp thrombospondin ( pmTSP-II) and their physiological functions were investigated in the black tiger shrimp Penaeus monodon. A molecular analysis of pmTSP-II demonstrated anchorage sites for N-linked glycans in both the chitin-binding and TSP3 domains. The presence of Man residues was verified by concanavalin A lectin histochemistry on the purified fraction of pmTSP-II (250 kDa with protease inhibitor)...
February 11, 2019: Molecular Reproduction and Development
Ai Ushiyama, Chathura Priyadarshana, Rangga Setiawan, Hitoshi Miyazaki, Naoto Ishikawa, Atsushi Tajima, Atsushi Asano
Despite knowledge that glucose metabolism is essential for the regulation of signaling cascades in the sperm that are pre-assembled into specific areas and function at multistage for fertilization, the physiological roles of glucose in avian sperm are poorly understood. Accumulated results of studies conducted in our laboratory and others indicate that sperm possess membrane microdomains, or membrane rafts, which play important roles in several processes, including the induction of acrosome reaction (AR). When characterizing proteomes associated with chicken sperm rafts, we observed marked enrichment of glucose transporter 3 (GLUT3)...
February 4, 2019: Biology of Reproduction
Bart Leemans, Tom Arjun Edgar Stout, Catharina De Schauwer, Sonia Heras, Hilde Nelis, Maarten Hoogewijs, Ann Van Soom, Bart M Gadella
In contrast to various other mammalian species, conventional in vitro fertilization (IVF) with horse gametes is not reliably successful. In particular, stallion spermatozoa fail to penetrate the zona pellucida, most likely due to incomplete activation of stallion spermatozoa (capacitation) under in vitro conditions. In other mammalian species, specific capacitation triggers have been described; unfortunately, none of these is able to induce full capacitation in stallion spermatozoa. Nevertheless, knowledge of capacitation pathways and their molecular triggers might improve our understanding of capacitation-related events observed in stallion sperm...
February 1, 2019: Reproduction: the Official Journal of the Society for the Study of Fertility
Tiane Ferreira de Castro, Antônio Sergio Varela Junior, Francine Ferreira Padilha, Daniela Droppa-Almeida, Graciela Quintana Saalfeld, Diego Martins Pires, Jessica Ribeiro Pereira, Carine Dahl Corcini, Elton Pinto Colares
Triphenyltin (TPT) is an organotin compound (OT), primarily used in agriculture and in the composition of antifouling paints for ships worldwide. Studies have showed its effects as an endocrine disrupter in several organisms by preventing enzymatic expression and causing reproductive toxicity. This study aimed to evaluate the effects of exposure to TPT, via breastfeeding, on reproductive physiology in the Calomys laucha species. The experimental design was compound of five groups, two controls and three with different doses of TPT...
January 31, 2019: Environmental Science and Pollution Research International
Kohei Umezu, Risa Yajima, Yuuki Hiradate, Rin Yanai, Takashi Numabe, Kenshiro Hara, Toshinori Oikawa, Kentaro Tanemura
Previously, we reported that neurotensin (NT), which is expressed in the uterus and oviduct, enhanced bovine sperm capacitation and acrosome reactions. As NT mRNA expression in bovine oviducts increases dramatically in the follicular phase, we hypothesized that NT modulates fertilization and subsequent conception in cattle. The objective of this study was to evaluate the effect of NT on embryo development and blastocyst quality. The rate of embryo cleavage was significantly increased by the addition of NT to the fertilization medium...
January 19, 2019: Journal of Reproduction and Development
Yusuke Aoki, Akira Tsujimura, Yuki Nagashima, Ippei Hiramatsu, Yuka Uesaka, Taiji Nozaki, Tatsuya Ogishima, Masato Shirai, Yukihiro Shoyama, Hiromitsu Tanaka, Shigeo Horie
Purpose: The direct effects of Lepidium meyenii (Maca) on sperm remain unclear. Herein, we examined the direct effect of Maca on in vitro fertilization. Methods: We examined the fertilization rate in a mouse model and the rate of acrosome reaction in sperm from transgenic mice expressing enhanced green fluorescent protein (EGFP) in a Maca extract-containing human tubal fluid (HTF) medium. Using human sperm, we assessed acrosome status via fluorescein isothiocyanate-conjugated peanut agglutinin (FITC-PNA) staining and performed detailed analysis using a sperm motility analysis system (SMAS)...
January 2019: Reproductive Medicine and Biology
Kejia Wu, Chunlei Mei, Yao Chen, Lidan Guo, Yuejin Yu, Donghui Huang
RESEARCH QUESTION: What is the effect of C-type natriuretic peptide (CNP) on human sperm capacitation in vitro and what is the mechanism of this effect? DESIGN: CNP/NPR-B expression in the female rat genital tract was examined by immunohistochemistry and western blot assay, and then the role of CNP in human sperm capacitation was determined. The signal transduction pathway of CNP in the process was determined to elucidate the regulation mechanism of CNP by enzyme-linked immunosorbent assay and flow cytometry...
January 5, 2019: Reproductive Biomedicine Online
Tao Luo, Hou-Yang Chen, Qian-Xing Zou, Tao Wang, Yi-Min Cheng, Hua-Feng Wang, Fang Wang, Zhong-Lin Jin, Ying Chen, Shi-Qi Weng, Xu-Hui Zeng
STUDY QUESTION: Are genetic abnormalities in CATSPER (cation channel of sperm) genes associated with idiopathic male infertility with normal semen parameters and, if so, how do they affect male fertility? SUMMARY ANSWER: A novel copy number variation (CNV) in CATSPER2 causes idiopathic male infertility with normal semen parameters by disrupting the ability of sperm to penetrate viscous media, undergo hyperactivation and respond to progesterone. WHAT IS KNOWN ALREADY: CATSPER is the principle Ca2+ channel mediating extracellular Ca2+ influx into spermatozoa...
January 10, 2019: Human Reproduction
Yulia Michailov, Eitan Lunenfeld, Joseph Kapilushnik, Shevach Friedler, Eckart Meese, Mahmoud Huleihel
Leukemia is one of the most common cancers in patients of reproductive age. It is well known that chemotherapy, used as anti-cancer therapy, adversely affects male fertility. Moreover, the negative effect of leukemia on sperm quality, even before chemotherapy treatment, has been reported. However, the mechanisms behind this disease's effect on sperm quality remains unknown. In this study, we examine the direct effect of leukemia and chemotherapy alone and in combination on sperm parameters and male fertility...
January 8, 2019: International Journal of Molecular Sciences
Kiyoshi Nagashima, Tomoyuki Usui, Tadashi Baba
Gene-knockout mice lacking ACRBP, a proacrosin-binding protein localized in the acrosome of sperm, have been shown to exhibit male subfertility, owing to abnormal formation of the acrosome. In this study, to elucidate the mechanism contributing to the subfertility phenotype, we examined the behavior of ACRBP-deficient mouse sperm in the female reproductive tract. When sperm that had migrated into the uterus and oviduct after mating were counted, the number of ACRBP-deficient sperm was noticeably smaller in the oviduct of mice post mating...
December 29, 2018: Journal of Reproduction and Development
Y Mu, C Jiang, M Jiao, Y Zhao, J Lv, Z Liu
Sperm-mediated gene transfer (SMGT) is based on the ability of spermatozoa to bind exoge- nous DNA and transfer it into oocytes by fertilization. However, SMGT is still undergoing opti- mization to improve its efficiency to produce transgenic animals. The acrosome reaction is neces- sary for spermatozoa to carry the exogenous DNA into oocytes. In this study, the effect of the acrosome reaction on the efficiency of spermatozoa carrying exogenous DNA was evalua- ted. The results showed that the efficiency of the acrosome reaction was significantly higher (p⟨0...
December 2018: Polish Journal of Veterinary Sciences
María Florencia Quevedo, Matías Alberto Bustos, Diego Masone, Carlos Marcelo Roggero, Diego Martín Bustos, Claudia Nora Tomes
Sperm must undergo the regulated exocytosis of its dense core granule (the acrosome reaction, AR) to fertilize the egg. We have previously described that Rabs3 and 27 are organized in a RabGEF cascade within the signaling pathway elicited by exocytosis stimuli in human sperm. Here, we report the identity and the role of two molecules that link these secretory Rabs in the RabGEF cascade: Rabphilin3a and GRAB. Like Rab3 and Rab27, GRAB and Rabphilin3a are present, localize to the acrosomal region and are required for calcium-triggered exocytosis in human sperm...
December 29, 2018: Biochimica et biophysica acta. Molecular cell research
Mrudhula Sajeevadathan, Murray J Pettitt, Mary Buhr
The endogenous steroid hormone ouabain induces capacitation of bull sperm acting through its receptor Na+ /K+ -ATPase on the sperm plasma membrane. Progesterone (P4) is believed to act through the sperm membrane P4 receptor (mPR) to induce non-genomic signalling leading to capacitation and/or acrosome reaction (AR) in the sperm of some species, but the exact nature of this receptor molecule on bull sperm is not known. In amphibian oocytes, P4 acts through the low-affinity ouabain binding site on Na+ /K+ -ATPase to induce signalling highly reminiscent of ouabain's signalling that initiates capacitation...
December 3, 2018: Theriogenology
A Swegen, N D Smith, Z Gibb, B J Curry, R J Aitken
BACKGROUND AND OBJECTIVES: Serine proteases are emerging as important players in the spermatozoon's acquisition of functional competence. This study aimed to characterize the serine protease testisin (PRSS21) in stallion spermatozoa, examining its surface expression, possible origins in the testis and epididymis, and changes in response to capacitation and acrosome reaction, as well as its capacity to form high molecular weight complexes and interact with other proteins. MATERIALS AND METHODS: The role of serine proteases in spontaneous capacitation and acrosome reaction of stallion spermatozoa was established using the serine protease inhibitor, AEBSF...
December 13, 2018: Andrology
Tereza Bosakova, Antonin Tockstein, Natasa Sebkova, Ondrej Simonik, Hana Adamusova, Jana Albrechtova, Tomas Albrecht, Zuzana Bosakova, Katerina Dvorakova-Hortova
17β-estradiol (estradiol) is a natural estrogen regulating reproduction including sperm and egg development, sperm maturation-called capacitation-and sperm⁻egg communication. High doses can increase germ cell apoptosis and decrease sperm count. Our aim was to answer the biological relevance of estradiol in sperm capacitation and its effect on motility and acrosome reaction to quantify its interaction with estrogen receptors and propose a model of estradiol action during capacitation using kinetic analysis...
December 12, 2018: International Journal of Molecular Sciences
Zeinab Fereshteh, Pradeepthi Bathala, Deni S Galileo, Patricia A Martin-DeLeon
Extracellular vesicles (EVs) were isolated by ultracentrifugation of vaginal luminal fluid (VLF) from superovulated mice and identified for the first time using transmission electron microscopy. Characterized by size and biochemical markers (CD9 and HSC70), EVs were shown to be both microvesicular and exosomal and were dubbed as "Vaginosomes" (VGS). Vaginal cross-sections were analyzed to visualize EVs in situ: EVs were present in the lumen and also embedded between squamous epithelial and keratinized cells, consistent with their endogenous origin...
December 7, 2018: Journal of Cellular Physiology
Wen Chen, Shi-Qi Weng, Meng-Ge Lv, Wen-Qiong Chen, Zhuo-Fei Bi, Hou-Yang Chen, Tao Luo, Hai Hu, Wen-Bo Liao
BACKGROUND/AIMS: β-Elemene is a bioactive sesquiterpene compound that exhibits a potent anti-tumor effect and is used in various clinical applications. However, little is known about its effect on the male reproductive system. The objective of this study was to investigate the in vitro actions of β-elemene on human sperm function and elucidate the underlying mechanism. METHODS: The cytotoxicity of β-elemene toward MCF-10A, MDA-MD-231, and A549 cells was evaluated with cell proliferation and colony formation assays...
2018: Cellular Physiology and Biochemistry
Carla Moros-Nicolás, Sophie Fouchécourt, Ghylène Goudet, Philippe Monget
Oviductal proteins play an important role in mammalian fertilization, as proteins from seminal fluid. However, in contrast with the latter, their phylogenetic evolution has been poorly studied. Our objective was to study in 16 mammals the evolution of 16 genes that encode oviductal proteins involved in at least one of the following steps: (1) sperm-oviduct interaction, (2) acrosome reaction, and/or (3) sperm-zona pellucida interaction. Most genes were present in all studied mammals. However, some genes were lost along the evolution of mammals and found as pseudogenes: annexin A5 (ANXA5) and deleted in malignant brain tumor 1 (DMBT1) in tarsier; oviductin (OVGP1) in megabat; and probably progestagen-associated endometrial protein (PAEP) in tarsier, mouse, rat, rabbit, dolphin, and megabat; prostaglandin D2 synthase (PTGDS) in microbat; and plasminogen (PLG) in megabat...
December 2018: Journal of Molecular Evolution
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