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Sperm maturation

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https://read.qxmd.com/read/30759213/crisp2-is-a-regulator-of-multiple-aspects-of-sperm-function-and-male-fertility
#1
Shuly Lim, Michelina Kierzek, Anne E O'Connor, Christoph Brenker, D Jo Merriner, Hidenobu Okuda, Marianna Volpert, Avinash Gaikwad, Deborah Bianco, David Potter, Ranganathan Prabhakar, Timo Strünker, Moira K O'Bryan
The Cysteine-RIch Secretory Proteins (CRISPs) are a group of proteins which show a pronounced expression biased to the male reproductive tract. While sperm encounter CRISPs at virtually all phases of sperm development and maturation, CRISP2 is the sole CRISP produced during spermatogenesis, wherein it is incorporated into the developing sperm head and tail. Within this study we test the necessity for CRISP2 in male fertility using Crisp2 loss-of-function mouse models. In doing so, we reveal a role for CRISP2 in establishing the ability of sperm to undergo the acrosome reaction, and in establishing a normal flagellum wave form...
February 13, 2019: Endocrinology
https://read.qxmd.com/read/30759202/transcription-factor-usf1-is-required-for-maintenance-of-germline-stem-cells-in-male-mice
#2
Imrul Faisal, Sheyla Cisneros-Montalvo, Geert Hamer, Minna Tuominen, Pirkka-Pekka Laurila, Manuela Tumiati, Matti Jauhiainen, Noora Kotaja, Jorma Toppari, Juho-Antti Mäkelä, Liisa Kauppi
A prerequisite for lifelong sperm production is that spermatogonial stem cells (SSCs) balance self-renewal and differentiation, yet factors required for this balance remain largely undefined. Using mouse genetics, we now demonstrate that the ubiquitously expressed transcription factor USF1 (Upstream stimulatory factor 1) is critical for the maintenance of SSCs. We show that USF1 is not only detected in Sertoli cells as previously reported, but also in SSCs. Usf1-deficient mice display progressive spermatogenic decline as a result of age-dependent loss of SSCs...
February 13, 2019: Endocrinology
https://read.qxmd.com/read/30756544/-separation-and-forensic-identification-of-sperm-from-cell-mixtures-using-anti-hlcn6-monoclonal-antibody-coupled-magnetic-beads
#3
Jiong Chen, Wei Feng, Fei Zhan
Human lipocalin 6 (hLCN6) is an epididymis-specific secretory protein. It binds to sperm and plays important role in sperm maturation. To explore the feasibility for isolating spermatozoa from mixed cells using anti-hLCN6 monoclonal antibody-conjugated immunomagnetic beads (anti-hLCN6 IMBs) and establish a new method for the separation of sperms from mixed stains, 2 sets of 30 cases of cell mixture suspensions and stains containing different proportions of sperm and epithelial cells were prepared. Biotin-labeled anti-hLCN6 monoclonal antibody (mAb) was incubated with the cell mixtures, and the spermatozoa were then isolated with avidin-coated IMBs...
January 25, 2019: Sheng Wu Gong Cheng Xue Bao, Chinese Journal of Biotechnology
https://read.qxmd.com/read/30746335/evaluating-the-potential-of-three-sperm-surface-antigens-as-egg-adhesion-biomarkers-for-human-sperm-selection
#4
Mahnaz Heidari, Sara Darbandi, Mahsa Darbani, Naser Amirjanati, Mahmood Bozorgmehr, Hojjat Zeraati, Mohammad Mehdi Akhondi, Mohammad Reza Sadeghi
Background: The selection of sperm with good genomic integrity and surface antigens is suggested for improving assisted reproductive technology (ART) outcome. The aim of this study was evaluating the heat shock protein (HSPA2), Dj-1 and serum amyloid P compound (SAP) three sperm surface proteomes as biomarkers for this purpose. Methods: In this study, semen samples were obtained from 114 men who presented at Avicenna Fertility Clinic for their treatment. The semen characteristics, DNA fragmentation Index (DFI), chromatin maturation index (CMI), biomarker levels, and their embryo quality were considered...
October 2018: Journal of Reproduction & Infertility
https://read.qxmd.com/read/30745236/assessment-of-the-calcium-releasing-machinery-in-oocytes-that-failed-to-fertilize-after-conventional-icsi-and-assisted-oocyte-activation
#5
Minerva Ferrer-Buitrago, Davina Bonte, Lien Dhaenens, Sanne Vermorgen, Yuechao Lu, Petra De Sutter, Björn Heindryckx
RESEARCH QUESTION: Can oocyte-related activation deficiencies be evaluated in oocytes that failed to fertilize after intracytoplasmic sperm injection (ICSI) combined with assisted oocyte activation (AOA)? DESIGN: Evaluation of the spindle-chromosome complexes and intracellular distribution of inositol trisphosphate type 1 receptors (IP3R1) in in-vitro matured (IVM) and failed-to-fertilize oocytes from patients undergoing AOA. Assessment of the oocyte-related Ca2+ releasing capacity in response to Ca2+ ionophores and sperm microinjection in oocytes that failed to fertilize after ICSI or ICSI-AOA...
December 23, 2018: Reproductive Biomedicine Online
https://read.qxmd.com/read/30743105/the-expression-and-localization-of-toll-like-receptors-2-4-5-and-9-in-the-epididymis-and-vas-deferens-of-a-adult-tom-cats
#6
Narin Liman, Emel Alan, Nusret Apaydın
Toll-like receptors (TLRs) are important molecules, which provide protection against infections of the reproductive tract. This study demonstrates for the first time the expression and localization patterns of TLRs in the caput, corpus and cauda segments of the epididymal duct (ED) and the vas deferens (VD) of adult domestic cats using immunohistochemistry and western blotting. While immunoblot analyses revealed relatively similar protein levels for TLRs 2, 4, 5, and 9 in three segments of the ED, the protein levels of TLR2 and TLR4 in the VD were found to be significantly higher than those measured in the ED segments (P < 0...
February 1, 2019: Theriogenology
https://read.qxmd.com/read/30742661/adverse-effects-of-paternal-obesity-on-the-motile-spermatozoa-quality
#7
Georges Raad, Joseph Azouri, Kamal Rizk, Nina S Zeidan, Jessica Azouri, Valérie Grandjean, Mira Hazzouri
Growing evidence suggests that paternal obesity may decrease male fertility potential. During infertility treatment with intra-cytoplasmic sperm injection (ICSI), a morphologically normal motile spermatozoon is injected into a mature egg, when possible. However, sperm motility and morphology per se do not reflect the sperm molecular composition. In this study, we aimed to assess the quality of motile spermatozoa in the context of obesity by analysing their conventional and molecular characteristics as well as their ability to promote early embryonic development...
2019: PloS One
https://read.qxmd.com/read/30741974/drosophila-pif1a-is-essential-for-spermatogenesis-and-is-the-homolog-of-human-ccdc157-a-gene-associated-with-idiopathic-noa
#8
Xin Yuan, Huimei Zheng, Yang Su, Pengfei Guo, Xiao Zhang, Qiang Zhao, Wanzhong Ge, Chen Li, Yongmei Xi, Xiaohang Yang
The dynamic process of spermatogenesis shows little variation between invertebrate models such as Drosophila, and vertebrate models such as mice and rats. In each case, germ stem cells undergo mitotic division to proliferate and then continue, via meiosis, through various stages of elongation and individualization from spermatogonia to spermatid to finally to form mature sperm. Mature sperm are then stored in the seminal vesicles for fertilization. Errors in any of these stages can lead to male infertility...
February 11, 2019: Cell Death & Disease
https://read.qxmd.com/read/30739277/correlation-of-testicular-ultrasonography-testicular-biometry-serum-testosterone-levels-and-seminal-attributes-in-pre-and-post-pubertal-age-for-breeding-soundness-evaluation-in-osmanabadi-bucks
#9
Umesh B Kumbhar, Sarita U Gulavane, Sandeep M Gaikwad, Raju R Shelar, Vaibhav P Deshpande, Ruth Rebeiro, Krishna Yadav
The present research work entitled "Correlation of testicular ultrasonography, testicular biometry, serum testosterone levels and seminal attributes in pre- and post-pubertal age for breeding soundness evaluation in Osmanabadi bucks" was undertaken in 18 healthy Osmanabadi bucks from the Instructional Livestock Farm Complex, Bombay Veterinary College, Mumbai, Maharashtra. The body weight (kg), scrotal circumference (cm) and testicular biometry (cm) of post-weaning 18 Osmanabadi male kids was recorded every 15 days from weaning, i...
February 9, 2019: Tropical Animal Health and Production
https://read.qxmd.com/read/30737686/membrane-microdomains-as-platform-to-study-membrane-associated-events-during-oogenesis-meiotic-maturation-and-fertilization-in-xenopus-laevis
#10
Ken-Ichi Sato, Alexander A Tokmakov
Studies on the egg plasma membrane-associated tyrosine kinase Src have shed light on the identity of the molecular machinery that is responsible for gamete interaction and possibly fusion in African clawed frog Xenopus laevis. Here we describe our protocol for identifying and analyzing molecular and cellular machinery that contributes to a variety of biological processes in the course of oogenesis, oocyte maturation, egg fertilization, and early embryogenesis in Xenopus. Our current special interest is to evaluate the hypothesis that the oocyte/egg membrane microdomain (MD)-associated uroplakin III-Src system is responsible for mediating sperm-egg membrane interaction/fusion signal to the oocyte/egg cytoplasm to initiate embryonic and zygotic development in this species...
2019: Methods in Molecular Biology
https://read.qxmd.com/read/30737682/culture-and-host-transfer-of-xenopus-oocytes-for-maternal-mrna-depletion-and-genome-editing-experiments
#11
Douglas W Houston
The early development of Xenopus critically depends on maternal components stored in the egg. Because important events such as axis formation are triggered immediately after fertilization, it is often desirable to perturb gene function before this occurs. Oocytes can be experimentally manipulated in vitro, prior to maturation, and subsequently fertilized or otherwise activated to develop, and then observed for any embryological defects. Available methods for fertilizing cultured oocytes include in vitro fertilization following oocyte vitelline envelope removal, nuclear transplantation, intracytoplasmic sperm injection, and transferring oocytes to the body cavity of ovulating host females (host transfer)...
2019: Methods in Molecular Biology
https://read.qxmd.com/read/30720094/aberrant-expression-of-sperm%C3%A2-specific-glycolytic-enzymes-are-associated-with-poor-sperm-quality
#12
Xuexia Liu, Qian Li, Wenjuan Wang, Fujun Liu
The energy required for normal sperm function is mainly generated by glycolysis and oxidative phosphorylation. Testis‑specific glycolytic enzymes are expressed in germ cells and present in mature spermatozoa. The objective of the present study was to investigate the association between aberrant expression of glycolytic enzymes and human sperm quality. In silico analysis of glyceraldehyde‑3‑phosphate dehydrogenase, testis‑specific (GAPDHS), phosphoglycerate kinase 2 (PGK2) and lactate dehydrogenase (LDHC) identified that they were exclusively expressed in post‑meiotic germ cells and this was validated in human testes using immunohistochemistry...
February 1, 2019: Molecular Medicine Reports
https://read.qxmd.com/read/30718080/male-fertility-preservation-in-dsd-xxy-pre-gonadotoxic-treatments-update-methods-ethical-issues-current-outcomes-future-directions
#13
REVIEW
Maria Grazia Giudice, Federico Del Vento, Christine Wyns
This paper aims at reviewing the fertility preservation strategies that could be considered in several conditions at risk of spermatogonial depletion such as 46,XY disorders of sexual development, Klinefelter syndrome and after gonadotoxic treatment in males highlighting current knowledge on diseases and processes involved in infertility as well as future directions along with their specific ethical issues. While sperm cryopreservation after puberty is the only validated technique for fertility preservation, for prepubertal boys facing gonadotoxic therapies or at risk of testicular tissue degeneration where testicular sperm is not present, cryopreservation of spermatogonial cells may be an option to ensure future parenthood...
January 23, 2019: Best Practice & Research. Clinical Endocrinology & Metabolism
https://read.qxmd.com/read/30707963/first-birth-of-a-healthy-infant-following-intra-cytoplasmic-sperm-injection-using-a-new-permeable-cryoprotectant-free-sperm-vitrification-protocol
#14
Llanos Medrano, María Enciso, María José Gomez-Torres, Jon Aizpurua
PURPOSE: The purpose of this study is to present the first birth of healthy infant born following ICSI using the new permeable cryoprotectant-free sperm vitrification protocol Easy-Sperm® . PRINCIPAL RESULTS: A 39 years old woman and his 40 years old partner underwent egg donation treatment at IVF-Spain Alicante (Spain). Half of the mature oocytes obtained from a young and healthy donor were fertilized by ICSI, using slow-frozen spermatozoa and the other half with vitrified spermatozoa...
January 29, 2019: Cryobiology
https://read.qxmd.com/read/30701548/sperm-maturation-and-male-tactic-specific-differences-in-ejaculates-in-plainfin-midshipman-fish-porichthys-notatus
#15
Jessica S Miller, Aneesh P H Bose, John L Fitzpatrick, Sigal Balshine
Using the plainfin midshipman fish Porichthys notatus, a species with alternative reproductive tactics (ART), we investigated how sperm maturation shapes sperm competitive abilities. We compared sperm performance and morphology before and after final sperm maturation by sampling sperm from the testes and stripped ejaculates of guarders and sneakers. In accordance with sperm competition risk theory, ejaculates from sneaker males had three times as much sperm as ejaculates from guarder males and sneaker males produced faster swimming sperm than guarder males, but this was only the case after final sperm maturation had occurred...
January 30, 2019: Journal of Fish Biology
https://read.qxmd.com/read/30689429/prenatal-stress-decreases-sperm-quality-mature-follicles-and-fertility-in-rats
#16
Diana García-Vargas, Lizbeth Juárez-Rojas, Susana Rojas Maya, Socorro Retana-Márquez
Prenatal stress disrupts reproductive function in females and males. These alterations have primarily been related to maternal corticosteroid fetal programming due to the stress response, affecting the fetus and causing long-lasting effects. The aim of this study was to investigate the influence of prenatal stress on male and female fertility. Dams were exposed to stress by immersion in cold water twice a day for the last week of gestation (days 15-21). In the adulthood, sperm quality, mature follicles, sexual hormones and fertility were assessed in female and male progeny...
January 28, 2019: Systems Biology in Reproductive Medicine
https://read.qxmd.com/read/30680919/deficiency-of-tppp2-a-factor-linked-to-oligoasthenozoospermia-causes-subfertility-in-male-mice
#17
Feng Zhu, Peipei Yan, Jingjing Zhang, Yiqiang Cui, Meimei Zheng, Yiwei Cheng, Yueshuai Guo, Xiaoyu Yang, Xuejiang Guo, Hui Zhu
Oligoasthenozoospermia is a major cause of male infertility; however, its etiology and pathogenesis are unclear and may be associated with specific gene abnormalities. This study focused on Tppp2 (tubulin polymerization promoting protein family member 2), whose encoded protein localizes in elongating spermatids at stages IV-VIII of the seminiferous epithelial cycle in testis and in mature sperm in the epididymis. In human and mouse sperm, in vitro inhibition of TPPP2 caused significantly decreased motility and ATP content...
January 24, 2019: Journal of Cellular and Molecular Medicine
https://read.qxmd.com/read/30678285/identification-of-premeiotic-meiotic-and-postmeiotic-cells-in-testicular-biopsies-without-sperm-from-sertoli-cell-only-syndrome-patients
#18
Maram Abofoul-Azab, Eitan Lunenfeld, Eliahu Levitas, Atif Zeadna, Johnny S Younis, Shalom Bar-Ami, Mahmoud Huleihel
Sertoli cell-only syndrome (SCOS) affects about 26.3⁻57.8% of azoospermic men, with their seminiferous tubules containing only Sertoli cells. Recently, it was reported that testicular biopsies from nonobstructive azoospermic (NOA) patients contained germ cells, and that sperm could be found in the tubules of 20% of SCOS patients using testicular sperm extraction technology. Since the patients without sperm in their testicular biopsies do not have therapy to help them to father a biological child, in vitro maturation of spermatogonial stem cells (SSCs) isolated from their testis is a new approach for possible future infertility treatment...
January 22, 2019: International Journal of Molecular Sciences
https://read.qxmd.com/read/30678254/maternal-protein-restriction-differentially-alters-the-expression-of-aqp1-aqp9-and-vegfr-2-in-the-epididymis-of-rat-offspring
#19
Marilia Martins Cavariani, Talita de Mello Santos, Dhrielly Natalia Pereira, Luiz Gustavo de Almeida Chuffa, Patricia Fernanda Felipe Pinheiro, Wellerson Rodrigo Scarano, Raquel Fantin Domeniconi
BACKGROUND: Maternal protein restriction causes sperm alterations in the offspring, most of which are associated with epididymal functions. Because fluid reabsorption/secretion dynamics in the epididymal environment play important roles in the process of sperm maturation and concentration, we investigated the effects of maternal protein restriction on the expression of aquaporins (AQP1 and AQP9), vascular endothelial growth factor (VEGFa), and its receptor VEGFr-2 in different stages of postnatal epididymal development...
January 22, 2019: International Journal of Molecular Sciences
https://read.qxmd.com/read/30675644/transcriptomics-of-arabidopsis-sperm-cells-at-single-cell-resolution
#20
Chandra Shekhar Misra, Mário R Santos, Mariana Rafael-Fernandes, Nuno P Martins, Marta Monteiro, Jörg D Becker
We present a detailed protocol for isolation of single sperm cells and transcriptome analysis to study variation in gene expression between sperm cells. Male gametophyte development in flowering plants begins with a microspore mother cell, which upon two consecutive cell divisions forms a mature pollen grain containing a vegetative nucleus and two sperm cells. Pollen development is a highly dynamic process, involving changes at both the transcriptome and epigenome levels of vegetative nuclei and the pair of sperm cells that have their own cytoplasm and nucleus...
January 24, 2019: Plant Reproduction
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