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grape metabolomic

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https://read.qxmd.com/read/30722864/hrmas-nmr-metabolomics-of-aglianicone-grapes-pulp-to-evaluate-terroir-and-vintage-effects-and-as-assessed-by-the-electromagnetic-induction-emi-technique-spatial-variability-of-vineyard-soils
#1
Pierluigi Mazzei, Giuseppe Celano, Assunta Maria Palese, Egidio Lardo, Marios Drosos, Alessandro Piccolo
Aglianicone is an autochthonous variety of black berried wine grape cultivated exclusively in southern Italy, but with limited information on the molecular composition of its grapes. HRMAS NMR spectroscopy was used to identify the primary metabolome of Aglianicone grape pulp from two different vineyards and the observed metabolomic changes reflected the local terroir conditions or harvesting years. Moreover, the berries pulp metabolome was related to Apparent Electrical Conductivity (ECa) maps deriving from on-site near-surface geophysical measurements of vineyard soils obtained by an electromagnetic induction (EMI) technique...
June 15, 2019: Food Chemistry
https://read.qxmd.com/read/30695987/comparative-metabolic-profiling-of-grape-skin-tissue-along-grapevine-berry-developmental-stages-reveals-systematic-influences-of-root-restriction-on-skin-metabolome
#2
Shuyan Duan, Yusen Wu, Ruifeng Fu, Lei Wang, Yujin Chen, Wenping Xu, Caixi Zhang, Chao Ma, Jianxin Shi, Shiping Wang
This research aimed to comparatively evaluate the influences of root restriction (RR) cultivation and traditional cultivation (RC) on grape berry skin metabolomics using a non-targeted metabolomics method. Two-hundred-and-ninety-one metabolites were annotated and the kinetics analyses showed that berry skin metabolome is stage- and cultivation-dependent. Our results showed that RR influences significantly the metabolomes of berry skin tissues, particularly on secondary metabolism, and that this effect is more obvious at pre-veraison stage, which was evidenced by the early and fast metabolic shift from primary to secondary metabolism...
January 28, 2019: International Journal of Molecular Sciences
https://read.qxmd.com/read/30685037/fingerprinting-of-traditionally-produced-red-wines-using-liquid-chromatography-combined-with-drift-tube-ion-mobility-mass-spectrometry
#3
Tim J Causon, Violeta Ivanova-Petropulos, Dragana Petrusheva, Elena Bogeva, Stephan Hann
The characterization of wine via MS-based metabolic fingerprinting techniques remains a challenging undertaking due to the large number of phenolic compounds that cannot be confidently annotated and identified within analytical workflows. The combination of high performance liquid chromatography with low-field drift tube ion mobility time-of-flight mass spectrometry (HPLC × IMS-TOFMS) offers potential for the confident characterization and fingerprinting of wine using a metabolomics-type workflow. In particular, the use of collision cross section values from low-field drift tube IMS using nitrogen as drift gas (DT CCSN2 ) in addition to retention time and a high resolution mass spectrum for putative compounds allows rugged statistical assessment and identity confirmation using CCS libraries (<0...
April 4, 2019: Analytica Chimica Acta
https://read.qxmd.com/read/30683739/yeast-cytosolic-thioredoxins-control-glycolysis-lipid-metabolism-and-protein-biosynthesis-under-winemaking-conditions
#4
Cecilia Picazo, Brian McDonagh, José Peinado, José A Bárcena, Emilia Matallana, Agustín Aranda
Thioredoxins are small proteins that regulate the cellular redox state, prevent oxidative damage and play an active role in its repair. Oxidative stress has proven to be of much relevance in biotechnological processes when Saccharomyces cerevisiae metabolism is mainly respiratory. During wine yeast starter production as active dry yeast cytosolic thioredoxin Trx2p is a key player in protecting metabolic enzymes from being oxidized by carbonylation. Less is known about the role of redox control during grape juice fermentation...
January 25, 2019: Applied and Environmental Microbiology
https://read.qxmd.com/read/30373017/combined-magnetic-resonance-imaging-and-high-resolution-spectroscopy-approaches-to-study-the-fertilization-effects-on-metabolome-morphology-and-yeast-community-of-wine-grape-berries-cultivar-nero-di-troia
#5
Alessandra Ciampa, Maria Teresa Dell'Abate, Alessandro Florio, Luigi Tarricone, Domenico Di Gennaro, Gianfranco Picone, Alessia Trimigno, Francesco Capozzi, Anna Benedetti
Effects of fertilization practices, mineral (M) and organo-mineral (OM), on molecular composition of Nero di Troia cultivar grape berries was studied using conventional chemical analysis, Magnetic Resonance Imaging (MRI) and 1 H NMR spectroscopy on intact berries and extracts, respectively, and through analysis of yeast species developed on grape skins. Plants vegetative status did not differ between the two fertilization practices, whereas some grape juice chemical characteristics differed in fertilized grapes...
February 15, 2019: Food Chemistry
https://read.qxmd.com/read/30366992/genomic-and-phenomic-analysis-of-hanseniaspora-vineae-provides-insights-for-understanding-yeast-fermentation-flavours-that-contribute-to-wine-quality
#6
Facundo Giorello, Maria Jose Valera, Valentina Martin, Andres Parada, Valentina Salzman, Laura Camesasca, Laura Fariña, Eduardo Boido, Karina Medina, Eduardo Dellacassa, Luisa Berna, Pablo S Aguilar, Albert Mas, Carina Gaggero, Francisco Carrau
Hanseniaspora is the main genus of the apiculate yeast group that represents about 70% of the grape-associated microflora. Hanseniaspora vineae is emerging as a promising species for quality wine production compared to other non- Saccharomyces Wines produced by H. vineae with Saccharomyces cerevisiae consistently exhibit more intense fruity flavours and complexity than wines produced by S. cerevisiae alone.In this work, genome sequencing, assembling and phylogenetic analysis of two strains of H. vineae shows that it is a member of the Saccharomyces complex and it diverged before the Whole Genome Duplication (WGD) event from this clade...
October 26, 2018: Applied and Environmental Microbiology
https://read.qxmd.com/read/30352003/the-compound-characteristics-comparison-ccc-approach-a-tool-for-improving-confidence-in-natural-compound-identification
#7
Luca Narduzzi, Jan Stanstrup, Fulvio Mattivi, Pietro Franceschi
Compound identification is the main hurdle in LC-HRMS-based metabolomics, given the high number of 'unknown' metabolites. In recent years, numerous in silico fragmentation simulators have been developed to simplify and improve mass spectral interpretation and compound annotation. Nevertheless, expert mass spectrometry users and chemists are still needed to select the correct entry from the numerous candidates proposed by automatic tools, especially in the plant kingdom due to the huge structural diversity of natural compounds occurring in plants...
November 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://read.qxmd.com/read/30174061/a-gc-ms-untargeted-metabolomics-approach-for-the-classification-of-chemical-differences-in-grape-juices-based-on-fungal-pathogen
#8
Claudia Schueuermann, Christopher C Steel, John W Blackman, Andrew C Clark, Lachlan J Schwarz, Javier Moraga, Isidro G Collado, Leigh M Schmidtke
Fungal bunch rot of grapes leads to production of detrimental flavour compounds, some of which are well characterised but others remain unidentified. The current study uses an untargeted metabolomics approach to classify volatile profiles of grape juices based on the presence of different fungal pathogens. Individual grape berries were inoculated with Botrytis cinerea, Penicillium expansum, Aspergillus niger or A. carbonarius. Grape bunches were inoculated and blended with healthy fruit, to provide 10% (w/w) infected juice...
January 1, 2019: Food Chemistry
https://read.qxmd.com/read/30096685/physiological-micro-morphological-and-metabolomic-analysis-of-grapevine-vitis-vinifera-l-leaf-of-plants-under-water-stress
#9
Yan-Lun Ju, Xiao-Feng Yue, Xian-Fang Zhao, Hui Zhao, Yu-Lin Fang
Grapes are one of the most important fruits because of their economic and nutritional benefits, and grapevines are widely cultivated in arid and semi-arid areas. Therefore, it is critical to study the mechanism by which grapevines respond to water stress. In this research, micro-morphological and metabolomic analyses were conducted to evaluate the effects of water stress on stomatal morphology and volatile compounds extracted from the leaves of grapevine plants. There were two treatments: well-watered plants (watered daily) and drought-stressed plants (no irrigation)...
September 2018: Plant Physiology and Biochemistry: PPB
https://read.qxmd.com/read/30064789/cell-wall-and-metabolite-composition-of-berries-of-vitis-vinifera-l-cv-thompson-seedless-with-different-firmness
#10
Baltasar Zepeda, Patricio Olmedo, Troy Ejsmentewicz, Pablo Sepúlveda, Iván Balic, Cristóbal Balladares, Joaquín Delgado-Rioseco, Claudia Fuentealba, Adrián A Moreno, Bruno G Defilippi, Claudio Meneses, Romina Pedreschi, Reinaldo Campos-Vargas
Firm berries are highly appreciated by table grape consumers. Cell wall composition is one of the main factors influencing the firmness of table grape berries. Nevertheless, the biological factors driving changes in berry firmness remain unclear. In the present work, we evaluated the firmness of berries of Vitis vinifera cv. Thompson Seedless. We selected two orchards displaying contrasting berry firmness and evaluated polar metabolites and cell wall composition. Our results suggest that berries from the soft orchard exhibited a higher accumulation of sugars at veraison whereas berries from the hard orchard accumulated the same sugars at harvest plus a higher amount of glucose monosaccharide at the cell wall...
December 1, 2018: Food Chemistry
https://read.qxmd.com/read/30032176/polyphenols-from-grape-and-blueberry-improve-episodic-memory-in-healthy-elderly-with-lower-level-of-memory-performance-a-bicentric-double-blind-randomized-placebo-controlled-clinical-study
#11
Julien Bensalem, Stéphanie Dudonné, Nicole Etchamendy, Hermine Pellay, Camille Amadieu, David Gaudout, Séverine Dubreuil, Marie-Eve Paradis, Sonia Pomerleau, Lucile Capuron, Carol Hudon, Sophie Layé, Yves Desjardins, Véronique Pallet
Polyphenols are promising nutritional bioactives exhibiting beneficial effect on age-related cognitive decline. The present study evaluated the effect of a polyphenol-rich extract from grape and blueberry (PEGB) on memory of healthy elderly subjects (60-70 years-old). A bicentric, randomized, double-blind, placebo-controlled trial was conducted with 215 volunteers receiving 600 mg/day of PEGB (containing 258 mg flavonoids) or a placebo for 6 months. The primary outcome was the CANTAB Paired Associate Learning (PAL), a visuospatial learning and episodic memory test...
July 19, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://read.qxmd.com/read/29989408/metabolomic-approach-for-the-authentication-of-berry-fruit-juice-by-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry-coupled-to-chemometrics
#12
Jiukai Zhang, Qiuhao Yu, Haiyan Cheng, Yiqiang Ge, Han Liu, Xingqian Ye, Ying Chen
Berry fruit juice, which is represented by blueberry and cranberry juice, has become increasingly popular due to its reported nutritional and health benefits. However, in markets, adulteration of berry fruit juice with cheaper substitutes is frequent. In the present study, a metabolomic approach for authentication of berry fruit juices by liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) was established. The global characterization of the berry fruit metabolome by information dependent acquisition directed LC-MS/MS coupled to a peak mining workflow by isotope pattern matching was reported...
August 1, 2018: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/29977248/field-based-metabolomics-of-vitis-vinifera-l-stems-provides-new-insights-for-genotype-discrimination-and-polyphenol-metabolism-structuring
#13
Kévin Billet, Benjamin Houillé, Thomas Dugé de Bernonville, Sébastien Besseau, Audrey Oudin, Vincent Courdavault, Guillaume Delanoue, Laurence Guérin, Marc Clastre, Nathalie Giglioli-Guivarc'h, Arnaud Lanoue
Grape accumulates numerous polyphenols with abundant health benefit and organoleptic properties that in planta act as key components of the plant defense system against diseases. Considerable advances have been made in the chemical characterization of wine metabolites particularly volatile and polyphenolic compounds. However, the metabotyping (metabolite-phenotype characterization) of grape varieties, from polyphenolic-rich vineyard by-product is unprecedented. As this composition might result from the complex interaction between genotype, environment and viticultural practices, a field experiment was setting up with uniform pedo-climatic factors and viticultural practices of growing vines to favor the genetic determinism of polyphenol expression...
2018: Frontiers in Plant Science
https://read.qxmd.com/read/29868039/constraint-based-modeling-highlights-cell-energy-redox-status-and-%C3%AE-ketoglutarate-availability-as-metabolic-drivers-for-anthocyanin-accumulation-in-grape-cells-under-nitrogen-limitation
#14
Eric Soubeyrand, Sophie Colombié, Bertrand Beauvoit, Zhanwu Dai, Stéphanie Cluzet, Ghislaine Hilbert, Christel Renaud, Lilly Maneta-Peyret, Martine Dieuaide-Noubhani, Jean-Michel Mérillon, Yves Gibon, Serge Delrot, Eric Gomès
Anthocyanin biosynthesis is regulated by environmental factors (such as light, temperature, and water availability) and nutrient status (such as carbon, nitrogen, and phosphate nutrition). Previous reports show that low nitrogen availability strongly enhances anthocyanin accumulation in non carbon-limited plant organs or cell suspensions. It has been hypothesized that high carbon-to-nitrogen ratio would lead to an energy excess in plant cells, and that an increase in flavonoid pathway metabolic fluxes would act as an "energy escape valve," helping plant cells to cope with energy and carbon excess...
2018: Frontiers in Plant Science
https://read.qxmd.com/read/29744293/comparative-metabolomics-and-glycolysis-enzyme-profiling-of-embryogenic-and-nonembryogenic-grape-cells
#15
Jonathan Parrilla, Cécile Gaillard, Jérémy Verbeke, Mickaël Maucourt, Radoslav A Aleksandrov, Florence Thibault, Pierrette Fleurat-Lessard, Yves Gibon, Dominique Rolin, Rossitza Atanassova
A novel biological model was created for the comparison of grapevine embryogenic cells (EC) and nonembryogenic cells (NEC) sharing a common genetic background but distinct phenotypes, when cultured on their respective most appropriate media. Cytological characterization, 1 H-NMR analysis of intracellular metabolites, and glycolytic enzyme activities provided evidence for the marked metabolic differences between EC and NEC. The EC were characterized by a moderate and organized cell proliferation, coupled with a low flux through glycolysis, high capacity of phosphoenolpyruvate carboxylase and glucokinase, and high oxygen consumption...
May 2018: FEBS Open Bio
https://read.qxmd.com/read/29734364/endophytic-fungi-specifically-introduce-novel-metabolites-into-grape-flesh-cells-in-vitro
#16
Li-Hua Huang, Ming-Quan Yuan, Xiu-Jin Ao, An-Yun Ren, Han-Bo Zhang, Ming-Zhi Yang
Since endophytes can affect metabolism of host plants, they are expected to be used to improve crop quality, especially for crops with organoleptic sensitive products such as wine grape. However, details of metabolic interactions between endophytes and host plants were less understood. In this work, we used high pressure liquid chromatography (HPLC) to analyze the metabolites of fruit flesh cells of grape treated with dual culture of different endophytic fungal strains (EFS). We observed that the dual-culture with different fungal strains show different metabolites composition in grape cells...
2018: PloS One
https://read.qxmd.com/read/29606438/application-of-sequential-and-orthogonalised-partial-least-squares-so-pls-regression-to-predict-sensory-properties-of-cabernet-sauvignon-wines-from-grape-chemical-composition
#17
Jun Niimi, Oliver Tomic, Tormod Næs, David W Jeffery, Susan E P Bastian, Paul K Boss
The current study determined the applicability of sequential and orthogonalised-partial least squares (SO-PLS) regression to relate Cabernet Sauvignon grape chemical composition to the sensory perception of the corresponding wines. Grape samples (n = 25) were harvested at a similar maturity and vinified identically in 2013. Twelve measures using various (bio)chemical methods were made on grapes. Wines were evaluated using descriptive analysis with a trained panel (n = 10) for sensory profiling. Data was analysed globally using SO-PLS for the entire sensory profiles (SO-PLS2), as well as for single sensory attributes (SO-PLS1)...
August 1, 2018: Food Chemistry
https://read.qxmd.com/read/29486461/kaolin-particle-film-application-stimulates-photoassimilate-synthesis-and-modifies-the-primary-metabolome-of-grape-leaves
#18
Artur Conde, Andreia Neves, Richard Breia, Diana Pimentel, Lia-Tânia Dinis, Sara Bernardo, Carlos Manuel Correia, Ana Cunha, Hernâni Gerós, José Moutinho-Pereira
Water scarcity is associated with extreme temperatures and high irradiance, and significantly and increasingly affects grapevine yield and quality. In this context, the foliar application of kaolin, a chemically inert mineral that greatly reflects ultraviolet and infrared radiations, as well as, in part, photosynthetically active radiation, has recently been shown to decrease photoinhibition in mature leaves. Here, the influence of this particle film on grapevine leaf metabolome and carbohydrate metabolism was evaluated...
April 2018: Journal of Plant Physiology
https://read.qxmd.com/read/29342836/cool-climate-red-wines-chemical-composition-and-comparison-of-two-protocols-for-%C3%A2-h-nmr-analysis
#19
Violetta Aru, Klavs Martin Sørensen, Bekzod Khakimov, Torben Bo Toldam-Andersen, Søren Balling Engelsen
This study investigates the metabolome of 26 experimental cool-climate wines made from 22 grape varieties using two different protocols for wine analysis by proton nuclear magnetic resonance (¹H-NMR) spectroscopy. The wine samples were analyzed as-is (wet) and as dried samples. The NMR datasets were preprocessed by alignment and mean centering. No normalization or scaling was performed. The "wet" method preserved the inherent properties of the samples and provided a fast and effective overview of the molecular composition of the wines...
January 13, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://read.qxmd.com/read/29314841/grape-berry-acclimation-to-excessive-solar-irradiance-leads-to-repartitioning-between-major-flavonoid-groups
#20
N Reshef, N Agam, A Fait
Warm viticulture regions are associated with inferior wines, resulting from the interaction between microclimate and fruit biochemistry. Solar irradiance triggers biosynthetic processes in the fruit and dominates its thermal balance. Therefore, deciphering its impact on fruit metabolism is pivotal to develop strategies for fruit protection and ameliorate its quality traits. Here, we modified light quality and intensity in the fruit-zone and integrated micrometeorology with grape and wine metabolomics, allowing a complete assessment, from field to bottle...
April 11, 2018: Journal of Agricultural and Food Chemistry
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