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grape transcriptome

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https://read.qxmd.com/read/30696402/elevated-co-2-concentration-promotes-photosynthesis-of-grape-vitis-vinifera-l-cv-pinot-noir-plantlet-in-vitro-by-regulating-rbcs-and-rca-revealed-by-proteomic-and-transcriptomic-profiles
#1
Xin Zhao, Wen-Fang Li, Ying Wang, Zong-Huan Ma, Shi-Jin Yang, Qi Zhou, Juan Mao, Bai-Hong Chen
BACKGROUND: Plant photosynthesis can be improved by elevated CO2 concentration (eCO2 ). In vitro growth under CO2 enriched environment can lead to greater biomass accumulation than the conventional in micropropagation. However, little is know about how eCO2 promotes transformation of grape plantlets in vitro from heterotrophic to autotrophic. In addition, how photosynthesis-related genes and their proteins are expressed under eCO2 and the mechanisms of how eCO2 regulates RbcS, Rca and their proteins have not been reported...
January 29, 2019: BMC Plant Biology
https://read.qxmd.com/read/30675233/transcriptome-analysis-of-pancreatic-cancer-cell-response-to-treatment-with-grape-seed-proanthocyanidins
#2
Weihua Wang, Leilei Zhan, Dongqi Guo, Yanju Xiang, Yu Zhang, Muxing Tian, Zhanjiang Han
Grape seed proanthocyanidins (GSPs) have been demonstrated to exhibit potential chemotherapeutic efficacy against various cancer types. To determine the underlying molecular mechanisms involved in GSP-induced apoptosis, the present study prepared pancreatic cancer (PC) cells samples, S3, S12 and S24, which were treated with 20 µg/ml GSPs for 3, 12 and 24 h, respectively. Control cell samples, C3, C12 and C24, were also prepared. Using RNA-sequencing, transcriptome comparisons were performed, which identified 966, 3,543 and 4,944 differentially-expressed genes (DEGs) in S3 vs...
February 2019: Oncology Letters
https://read.qxmd.com/read/30672093/dominance-of-wine-saccharomyces-cerevisiae-strains-over-s-kudriavzevii-in-industrial-fermentation-competitions-is-related-to-an-acceleration-of-nutrient-uptake-and-utilization
#3
Javier Alonso-Del-Real, Roberto Pérez-Torrado, Amparo Querol, Eladio Barrio
Grape must is a sugar-rich habitat for a complex microbiota which is replaced by Saccharomyces cerevisiae strains during the first fermentation stages. Interest on yeast competitive interactions has recently been propelled due to the use of alternative yeasts in the wine industry to respond to new market demands. The main issue resides in the persistence of these yeasts due to the specific competitive activity of S. cerevisiae. To gather deeper knowledge of the molecular mechanisms involved, we performed a comparative transcriptomic analysis during fermentation carried out by a wine S...
January 22, 2019: Environmental Microbiology
https://read.qxmd.com/read/30647106/-the-role-of-uv-b-light-on-small-rna-activity-during-grapevine-berry-development
#4
Sukumaran Sunitha, Rodrigo Loyola, José Antonio Alcalde, Patricio Arce-Johnson, José Tomás Matus, Christopher D Rock
We explored the effects of ultraviolet B radiation (UV-B) on the developmental dynamics of microRNAs and phased small-interfering-RNA (phasi-RNAs)-producing loci by sequencing small RNAs in vegetative and reproductive organs of grapevine ( Vitis vinifera L.). In particular, we tested different UV-B conditions in in vitro -grown plantlets (high-fluence exposition) and in berries from field-grown (radiation filtering) and greenhouse-grown (low- and high-fluence expositions) adult plants throughout fruit development and ripening...
January 15, 2019: G3: Genes—Genomes—Genetics
https://read.qxmd.com/read/30642874/iso-seq-allows-genome-independent-transcriptome-profiling-of-grape-berry-development
#5
Andrea Minio, Mélanie Massonnet, Rosa Figueroa-Balderas, Amanda M Vondras, Barbara Blanco-Ulate, Dario Cantu
Transcriptomics has been widely applied to study grape berry development. With few exceptions, transcriptomic studies in grape are performed using the available genome sequence, PN40024, as reference. However, differences in gene content among grape accessions, which contribute to phenotypic differences among cultivars, suggest that a single reference genome does not represent the species' entire gene space. Though whole genome assembly and annotation can reveal the relatively unique or "private" gene space of any particular cultivar, transcriptome reconstruction is a more rapid, less costly, and less computationally intensive strategy to accomplish the same goal...
January 14, 2019: G3: Genes—Genomes—Genetics
https://read.qxmd.com/read/30629640/comparison-of-transcriptional-expression-patterns-of-phenols-and-carotenoids-in-kyoho-grapes-under-a-two-crop-a-year-cultivation-system
#6
Guo Cheng, Sihong Zhou, Jin Zhang, Xiaoyun Huang, Xianjin Bai, Taili Xie, Rongrong Guo, Jinbiao Liu, Huan Yu, Linjun Xie
To fully utilize the characteristic climatic conditions in the southern region of China, a two-crop-a-year cultivation system technique for 'Kyoho' grape was developed during the past decade. After summer harvest in June, appropriate pruning and chemical treatments promote flowering and fruiting, which enables a second harvest in late December. Due to climatic differences between the two crop growing seasons, grape phenol and carotenoid metabolism differ greatly. The reported study analyzed the transcriptome of the carotenoid and phenylpropanoid/flavonoid pathways in grapes at four different stages during the two growing seasons...
2019: PloS One
https://read.qxmd.com/read/30555495/absolute-quantification-of-grapevine-red-blotch-virus-in-grapevine-leaf-and-petiole-tissues-by-proteomics
#7
Natasha Buchs, Sophie Braga-Lagache, Anne-Christine Uldry, Justine Brodard, Christophe Debonneville, Jean-Sébastien Reynard, Manfred Heller
Grapevine red blotch is a recently identified viral disease that was first recognized in the Napa Valley of California. Infected plants showed foliar symptoms similar to leafroll, another grapevine viral disease, on vines testing negative for known grapevine leafroll-associated virus. Later, the Grapevine red blotch virus (GRBV) was independently discovered in the US states of California and New York and was demonstrated to be the causal agent of red blotch disease. Due to its wide occurrence in the United States, vector transmission, and impacts on grape industry, this virus has the potential to cause serious economic losses...
2018: Frontiers in Plant Science
https://read.qxmd.com/read/30473420/active-rearrangements-in-the-cell-wall-follow-polymer-concentration-during-postharvest-withering-in-the-berry-skin-of-vitis-vinifera-cv-corvina
#8
Marianna Fasoli, Rossana Dell'Anna, Alessandra Amato, Raffaella Balestrini, Silvia Dal Santo, Francesca Monti, Sara Zenoni
During grape postharvest withering, a worldwide practice used to produce important high-quality wines, the solute concentration increases due to dehydration, and many organoleptic and quality traits, especially related to the berry skin, are affected in a cultivar-specific manner. Nevertheless, a complete comprehension of the underlying processes is still lacking. In this work, we applied ATR-FTIR micro-spectroscopy combined with PCA to monitor cell wall biochemical changes at three stages during postharvest withering on the internal and external sides of the berry skin of the Vitis vinifera cv...
November 19, 2018: Plant Physiology and Biochemistry: PPB
https://read.qxmd.com/read/30425701/transcriptome-and-resistance-related-genes-analysis-of-botrytis-cinerea-b05-10-strain-to-different-selective-pressures-of-cyprodinil-and-fenhexamid
#9
Xuegui Wang, Changwei Gong, Yun Zhao, Litao Shen
The pathogen Botrytis cinerea is a very dangerous pathogen that infects many economically important crops such as grape, strawberry, tomato, and eggplant. Cyprodinil, a pyrimidine amine fungicide, and fenhexamid, an amide fungicide, are new reagents for controlling gray mold with special efficacy. It is necessary to understand the change trends in the toxicological and physiological characteristics of B. cinerea with successive selective pressures of cyprodinil and fenhexamid to elongate the serving life of these fungicides for effective disease control...
2018: Frontiers in Microbiology
https://read.qxmd.com/read/30366992/genomic-and-phenomic-analysis-of-hanseniaspora-vineae-provides-insights-for-understanding-yeast-fermentation-flavours-that-contribute-to-wine-quality
#10
Facundo Giorello, Maria Jose Valera, Valentina Martin, Andres Parada, Valentina Salzman, Laura Camesasca, Laura Fariña, Eduardo Boido, Karina Medina, Eduardo Dellacassa, Luisa Berna, Pablo S Aguilar, Albert Mas, Carina Gaggero, Francisco Carrau
Hanseniaspora is the main genus of the apiculate yeast group that represents about 70% of the grape-associated microflora. Hanseniaspora vineae is emerging as a promising species for quality wine production compared to other non- Saccharomyces Wines produced by H. vineae with Saccharomyces cerevisiae consistently exhibit more intense fruity flavours and complexity than wines produced by S. cerevisiae alone.In this work, genome sequencing, assembling and phylogenetic analysis of two strains of H. vineae shows that it is a member of the Saccharomyces complex and it diverged before the Whole Genome Duplication (WGD) event from this clade...
October 26, 2018: Applied and Environmental Microbiology
https://read.qxmd.com/read/30360570/rna-sequencing-based-whole-transcriptome-analysis-of-friesian-cattle-fed-with-grape-pomace-supplemented-diet
#11
Marco Iannaccone, Ramy Elgendy, Mery Giantin, Camillo Martino, Daniele Giansante, Andrea Ianni, Mauro Dacasto, Giuseppe Martino
Grape pomace (GPO), the main by-product of the wine making process, is a rich source of polyphenols with potent antioxidant properties. Recently, GPO has emerged as a potential feed additive in livestock nutrition, with several reports describing its beneficial effects on animals' overall health status or production traits. However, little is known about it from a molecular biology standpoint. In the present study, we report the first RNA sequencing-based whole-transcriptome profiling of Friesian calves fed with a GPO-supplemented diet...
October 23, 2018: Animals: An Open Access Journal From MDPI
https://read.qxmd.com/read/30348329/ethylene-receptors-and-related-proteins-in-climacteric-and-non-climacteric-fruits
#12
REVIEW
Yi Chen, Jérôme Grimplet, Karine David, Simone Diego Castellarin, Javier Terol, Darren C J Wong, Zhiwei Luo, Robert Schaffer, Jean-Marc Celton, Manuel Talon, Gregory Alan Gambetta, Christian Chervin
Fruits have been traditionally classified into two categories based on their capacity to produce and respond to ethylene during ripening. Fruits whose ripening is associated to a peak of ethylene production and a respiration burst are referred to as climacteric, while those that are not are referred to as non-climacteric. However, an increasing body of literature supports an important role for ethylene in the ripening of both climacteric and non-climacteric fruits. Genome and transcriptomic data have become available across a variety of fruits and we leverage these data to compare the structure and transcriptional regulation of the ethylene receptors and related proteins...
November 2018: Plant Science: An International Journal of Experimental Plant Biology
https://read.qxmd.com/read/30257181/transcriptome-profiling-and-identification-of-the-functional-genes-involved-in-berry-development-and-ripening-in-vitis-vinifera
#13
Qian Ma, Jingli Yang
The length of berry lag phase determines the overall time needed for grape berries to get mature, but the functional gene networks in this phase have not been well documented. In order to reveal the origin of the somatic variation and regulation mechanism of grape berry development and ripening, an early ripening mutant of Vitis vinifera with a shorter lag phase was used for transcriptome profiling. The RNA-seq results revealed that 2021 and 2470 genes were up- and down-regulated, respectively, in the early ripening mutant compared to the wild type...
January 5, 2019: Gene
https://read.qxmd.com/read/30110413/analysis-of-the-regulation-networks-in-grapevine-reveals-response-to-waterlogging-stress-and-candidate-gene-marker-selection-for-damage-severity
#14
Xudong Zhu, Xiaopeng Li, Songtao Jiu, Kekun Zhang, Chen Wang, Jinggui Fang
Owing to the climate change impacts, waterlogging is one of the most hazardous abiotic stresses to crops, which also can result in a serious reduction in the quantity and quality of grape berry and wine production during the rainy season. Therefore, the exploration of the response mechanism of grape to waterlogging is necessary, for which the analysis of the transcriptomic regulation networks of grapevine leaves in response to waterlogging stress was carried out. In this study, 12 634 genes were detected in both waterlogging stress and control grapevine plants, out of which 6837 genes were differentially expressed...
June 2018: Royal Society Open Science
https://read.qxmd.com/read/30082597/transcriptomic-analyses-of-root-restriction-effects-on-phytohormone-content-and-signal-transduction-during-grape-berry-development-and-ripening
#15
Feng Leng, Jinping Cao, Shiping Wang, Ling Jiang, Xian Li, Chongde Sun
Phytohormones strongly influence growth, development and nutritional quality of agricultural products by modulating molecular and biochemical changes. The purpose of the present study was to investigate the influence of root restriction (RR) treatment on the dynamic changes of main phytohormones during the berry development and ripening of "Summer Black" early ripening seedless grape ( Vitis vinifera × V. labrusca ), and to analyze the changes in the biosynthesis and signal transduction pathways of phytohormones by transcriptomics...
August 6, 2018: International Journal of Molecular Sciences
https://read.qxmd.com/read/30046943/transcriptome-analysis-of-grapevine-under-salinity-and-identification-of-key-genes-responsible-for-salt-tolerance
#16
Priyanka Das, Arun Lahiri Majumder
The negative effects of soil salinity towards grape yield depend upon salt concentration, cultivar type, developmental stage, and rootstock. Thompson Seedless variety of grape plant is considered moderately sensitive to salinity when grown upon its own root stock. In recent epoch, identification of key genes responsive to salinity offers hope to generate salinity-tolerant crop plants by their overexpression through genetic manipulation. In the present report, salt responsive transcriptome analysis of Thompson Seedless grape variety was done to identify vital genes involved in salinity tolerance which could be used further to generate salt liberal grape plant or other crop plants...
July 19, 2018: Functional & Integrative Genomics
https://read.qxmd.com/read/30030201/identification-of-candidate-genes-involved-in-anthocyanin-accumulation-in-the-peel-of-jaboticaba-myrciaria-cauliflora-fruits-by-transcriptomic-analysis
#17
Ya-Ling Zhang, Zhi-Zhen Fang, Xin-Fu Ye, Shao-Lin Pan
Jaboticaba is a grape-like fruit that accumulates high levels of anthocyanins in the peel and is proposed as a good source of functional pigments. However, the molecular mechanisms underlying anthocyanin accumulation in jaboticaba peel remains to be elucidated. In this study, we employed RNA-seq technique to compare the transcriptomic differences between green-colored and black-colored jaboticaba peels. Over 5 million high-quality reads were assembled into 62,190 unigenes with an average length of 737 bp, 29,320 (47...
November 15, 2018: Gene
https://read.qxmd.com/read/29920074/characterization-of-gene-expression-profile-phenolic-composition-and-antioxidant-capacity-in-red-fleshed-grape-berries-and-their-wines
#18
COMPARATIVE STUDY
Qianyu Yue, Lili Xu, Guangqing Xiang, Xin Yu, Yuxin Yao
Gene expression profile, phenolic composition, and antioxidant capacity were evaluated in red-fleshed berries and their wines (RF berries and wines) from new grape genotypes. Transcriptomic analysis revealed that ten metabolic pathways involved in polyphenol synthesis and catabolism were significantly altered, and 13 genes related to the biosynthesis and transport of phenolics were largely upregulated in RF berries compared to that of Cabernet Sauvignon (CS). Expression of MybA1 was associated with anthocyanin accumulation in red flesh...
July 11, 2018: Journal of Agricultural and Food Chemistry
https://read.qxmd.com/read/29626310/ga-3-application-in-grapes-vitis-vinifera-l-modulates-different-sets-of-genes-at-cluster-emergence-full-bloom-and-berry-stage-as-revealed-by-rna-sequence-based-transcriptome-analysis
#19
Anuradha Upadhyay, Smita Maske, Satisha Jogaiah, Narendra Y Kadoo, Vidya S Gupta
In grapes (Vitis vinifera L.), exogenous gibberellic acid (GA3 ) is applied at different stages of bunch development to achieve desirable bunch shape and berry size in seedless grapes used for table purpose. RNA sequence-based transcriptome analysis was used to understand the mechanism of GA3 action at cluster emergence, full bloom, and berry stage in table grape variety Thompson Seedless. At cluster emergence, rachis samples were collected at 6 and 24 h after application of GA3 , whereas flower clusters and berry samples were collected at 6, 24, and 48 h after application at full bloom and 3-4 mm berry stages...
July 2018: Functional & Integrative Genomics
https://read.qxmd.com/read/29566077/physiological-characterization-of-leaf-and-internode-after-bud-break-in-japanese-indigenous-koshu-grape-by-comparative-rna-sequencing-analysis
#20
Shinichi Enoki, Yu Hamaguchi, Shunji Suzuki, Hiroyuki Fujisawa, Tomoki Hattori, Kayo Arita, Chiho Yamaguchi, Masachika Mikami, Shu Nagasaka, Keisuke Tanaka
Koshu is indigenous to Japan and considered the most important wine grape in Japan. Koshu grape berry possesses characteristics that make it unique from European V. vinifera as wine grape. However, the physiological characteristics of Koshu leaf and internode remain unknown. An understanding of those characteristics would contribute to improvements in Koshu cultivation, thereby enhancing grape berry and wine quality. To identify the genes responsible for the physiological characteristics of Koshu, we comprehensively analyzed leaf and internode differences at the transcriptome level between Koshu and Pinot Noir by RNA sequencing...
2018: PloS One
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