Jennifer S McLeod, Joseph T Church, Megan A Coughlin, Benjamin Carr, Clinton Poling, Ellery Sarosi, Elena M Perkins, Matias Caceres Quinones, Pavel Hala, Raja Rabah, Elizabeth Freiheit, Alvaro Rojas-Pena, Robert H Bartlett, George B Mychaliska
INTRODUCTION: The purpose of this study is to evaluate splenic effects during artificial placenta (AP) support. METHODS: AP lambs (118-121 d, n = 14) were delivered and placed on the AP support for a goal of 10-14 days. Cannulation used right jugular drainage and umbilical vein reinfusion. Early (ETC; 115-120 d; n = 7) and late (LTC; 125-131 d; n = 7) tissue controls were delivered and immediately sacrificed. Spleens were formalin fixed, H&E stained, and graded for injury, response to inflammation, and extramedullary hematopoiesis (EMH)...
March 1, 2019: Journal of Pediatric Surgery