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JOURNAL ARTICLE
Cong Zhu, Tom Smith, Joseph McNulty, Amy L Rayla, Abirami Lakshmanan, Arndt F Siekmann, Matthew Buffardi, Xiangdong Meng, Jimann Shin, Arun Padmanabhan, Daniel Cifuentes, Antonio J Giraldez, A Thomas Look, Jonathan A Epstein, Nathan D Lawson, Scot A Wolfe
Zinc-finger nucleases (ZFNs) allow targeted gene inactivation in a wide range of model organisms. However, construction of target-specific ZFNs is technically challenging. Here, we evaluate a straightforward modular assembly-based approach for ZFN construction and gene inactivation in zebrafish. From an archive of 27 different zinc-finger modules, we assembled more than 70 different zinc-finger cassettes and evaluated their specificity using a bacterial one-hybrid assay. In parallel, we constructed ZFNs from these cassettes and tested their ability to induce lesions in zebrafish embryos...
October 2011: Development
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