Yiying Wang, Roberta A Mittelstaedt, Rebecca Wynne, Ying Chen, Xuefei Cao, Levan Muskhelishvili, Kelly Davis, Timothy W Robison, Wei Sun, Elizabeth K Schmidt, Thomas H Smith, Zachary K Norgaard, Charles C Valentine, Jeffry Yaplee, Lindsey N Williams, Jesse J Salk, Robert H Heflich
The organotypic human air-liquid-interface (ALI) airway tissue model has been used as an in vitro cell culture system for evaluating the toxicity of inhaled substances. ALI airway cultures are highly differentiated, which has made it challenging to evaluate genetic toxicology endpoints. In the current study, we assayed DNA damage with the high-throughput CometChip assay and quantified mutagenesis with Duplex Sequencing, an error-corrected next-generation sequencing method capable of detecting a single mutation per 107 base pairs...
May 29, 2021: Environmental and Molecular Mutagenesis