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clinical chemistry

J Lee, V A Eley, K H Wyssusek, E Coonan, M Way, J Cohen, J Rowell, A A van Zundert
BACKGROUND: Formal reference ranges for rotational thromboelastometry (ROTEM®) in pregnancy have not been obtained in the recommended minimum sample size of 120. This prospective observational study aimed to establish baseline parameters in an Australian population of women undergoing elective caesarean delivery. The secondary aim was to compare these reference ranges with those from prior studies and the manufacturer. METHODS: Women undergoing elective caesarean delivery at term were included if they were at term, with normal body mass index and had no conditions affecting coagulation...
January 16, 2019: International Journal of Obstetric Anesthesia
Valeria M Nurchi, Maria de Guadalupe Jaraquemada-Pelaez, Guido Crisponi, Joanna I Lachowicz, Rosita Cappai, Lurdes Gano, M Amelia Santos, Andrea Melchior, Marilena Tolazzi, Massimiliano Peana, Serenella Medici, Maria Antonietta Zoroddu
This work presents the simple and low cost synthesis of a new tripodal ligand, in which three units of kojic acid are coupled to a tris(2-aminoethyl)amine (tren) backbone molecule. The protonation equilibria, together with the complex formation equilibria of this ligand with Fe3+ , Al3+ , Cu2+ and Zn2+ ions were studied. The complementary use of potentiometric, spectrophotometric and NMR techniques, and of Density Functional Theory (DFT) calculations, has allowed a thorough characterization of the different species involved in equilibrium...
February 6, 2019: Journal of Inorganic Biochemistry
Jake P Newsom, Karin A Payne, Melissa D Krebs
Biopolymer microgels are emerging as a versatile tool for aiding in the regeneration of damaged tissues due to their biocompatible nature, tunable microporous structure, ability to encapsulate bioactive factors, and tailorable properties such as stiffness and composition. These properties of microgels, along with their injectability, have allowed for their utilization in a multitude of different tissue engineering applications. Controlled release of growth factors, antibodies, and other bioactive factors from microgels have demonstrated their capabilities as transporters for essential bioactive molecules necessary for guiding tissue reconstruction...
February 12, 2019: Acta Biomaterialia
Antonija Perović, Maja Dolčić
The aim of this study was to examine the influence of hemolysis on 25 clinical chemistry parameters and to compare the resulting bias with clinically significant differences and the manufacturer's specifications. Using freeze-thawing of the treated blood aliquot of each subject (N = 17), four hemolysis levels were prepared with hemolysis index (HI) and hemoglobin concentration as follows: (+)=0.5-0.99 g/L, (2+)=1-1.99 g/L, (3+)=2-2.99 g/L and (4+)=3-4.99 g/L. All analytes were tested on the Beckman Coulter AU480 analyzer using proprietary reagents...
February 15, 2019: Scandinavian Journal of Clinical and Laboratory Investigation
Travis J Carlson, Bradley T Endres, Eugénie Bassères, Anne J Gonzales-Luna, Kevin W Garey
Ridinilazole is a novel antibiotic being developed for the treatment of Clostridioides difficile infection (CDI). Ridinilazole has completed two phase II trials and phase III trials which are denoted Ri-CoDIFy 1 and 2, are planned ( identifiers: NCT03595553 and NCT03595566). Areas covered: This article covers the chemistry, mechanism of action, in vitro microbiology versus C. difficile and host microbiota, pre-clinical and clinical efficacy, pharmacokinetics, pharmacodynamics and safety and tolerability of ridinilazole...
February 15, 2019: Expert Opinion on Investigational Drugs
Gang Li, Raymond Moellering
Synthesis of antibody-oligonucleotide conjugates has enabled the development of highly sensitive bioassays for specific epitopes in the laboratory and clinic. Most synthetic schemes to generate these hybrid molecules require expensive reagents, significant quantities of input antibody, and multi-step purification routes, thus limiting widespread application. Here we report a facile and robust conjugation strategy that involves "plug-and-play" antibody conjugation with succinimidyl-functionalized oligonucleotides, which are high-yielding and compatible for use directly after buffer exchange...
February 14, 2019: Chembiochem: a European Journal of Chemical Biology
Maximilian Kittel, Peter Findeisen, Beniam Ghebremedhin, Thomas Miethke, Alexander Grundt, Parviz Ahmad-Nejad, Michael Neumaier
Background The increasing number of multi-drug resistant (MDR) bacteria provides enormous challenges for choosing an appropriate antibiotic therapy in the early phase of sepsis. While bacterial identification has been greatly accelerated by the introduction of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS), the antibiotic susceptibility testing (AST) remains time-consuming. Here, we present a rapid susceptibility testing method for testing Gram-negative bacteria, exemplarily validated for Escherichia coli and Klebsiella spp...
February 14, 2019: Clinical Chemistry and Laboratory Medicine: CCLM
Muhammad Asif, Ayesha Aziz, Zhengyun Wang, Ghazala Ashraf, Junlei Wang, Hanbo Luo, Xuedong Chen, Fei Xiao, Hongfang Liu
The precise monitoring of H2S has aroused immense research interests in biological and biomedical fields since it is exposed as third endogenous gasotransmitter. Hence, there is an urgent requisite to explore ultrasensitive and economical H2S detection system. Herein, we report a simple strategy to configure extremely sensitive electrochemical sensor with 2D nanosheet-shaped layered double hydroxide (LDH) wrapped carbon nanotubes (CNTs) nanohybrid (CNTs@LDH), where a series of CNTs@CuMn-LDH nanohybrids with varied amount of LDH nanosheets grafted on conductive CNTs backbone has been synthesized via facile co-precipitation approach...
February 14, 2019: Analytical Chemistry
(no author information available yet)
1,1,1,3,3,3-Hexamethyldisilazane (HMDZ) is used industrially to treat the surface of silica, as an intermediate adhesion promoter or silylating agent in the semiconductor industry, as a chemical modifier of inorganic fillers, and as a water scavenger silicone sealant. In animal studies, HMDZ is considered to be slightly to at most moderately toxic following acute administration via oral, dermal, and inhalation routes of exposure. HMDZ is neither an eye irritant nor was it dermally irritating under semiocclusive conditions; however, it caused dermal necrosis in two studies under occlusive conditions...
February 13, 2019: Toxicology and Industrial Health
Philippe Bertrand, Christophe Blanquart, Valérie Héroguez
Fast clearance, metabolism, and systemic toxicity are major limits for the clinical use of anti-cancer drugs. Histone deacetylase inhibitors (HDACi) present these defects, despite displaying promising anti-tumor properties on tumor cells in vitro and in in vivo models of cancer. The specific delivery of anti-cancer drugs into the tumor should improve their clinical benefit by limiting systemic toxicity and by increasing the anti-tumor effect. This paper deals with the synthesis of the polymeric nanoparticle platform, which was produced by Ring-Opening Metathesis Polymerization (ROMP), able to release anti-cancer drugs in dispersion, such as histone deacetylase inhibitors, into mesothelioma tumors...
February 12, 2019: Biomolecules
Katia Regina Vasconcelos, Juliana Arid, Silvane Evangelista, Sara Oliveira, André Luiz Dutra, Lea Assed Bezerra Silva, Raquel Assed Bezerra Segato, Alexandre Rezende Vieira, Paulo Nelson-Filho, Erika Calvano Küchler
The aim of this study was to investigate the association between genetic polymorphisms in MMP8, MMP13, and MMP20 with caries experience and developmental defects of enamel (DDE) in children from the Amazon region of Brazil. Den tal caries and DDE data were collected through clinical examination from 216 children. Genomic DNA was extracted from saliva, and genotyping of selected polymorphisms in MMP8 (rs17099443 and rs3765620), MMP13 (rs478927 and rs2252070), and MMP20 (rs1784418) was performed using TaqMan chemistry and endpoint analysis...
February 13, 2019: Caries Research
Jasmijn A van Balveren, Wilhelmine P H G Verboeket-van de Venne, Lale Erdem-Eraslan, Albert J de Graaf, Annemarieke E Loot, Ruben E A Musson, Wytze P Oosterhuis, Martin P Schuijt, Heleen van der Sijs, Rolf J Verheul, Holger K de Wolf, Ron Kusters, Rein M J Hoedemakers
Background Knowledge of possible drug-laboratory test interactions (DLTIs) is important for the interpretation of laboratory test results. Test results may be affected by physiological or analytical drug effects. Failure to recognize these interactions may lead to misinterpretation of test results, a delayed or erroneous diagnosis or unnecessary extra tests or therapy, which may harm patients. Content Thousands of interactions have been reported in the literature, but are often fragmentarily described and some papers even reported contradictory findings...
February 12, 2019: Diagnosis
Yabin Zhang, Lin Zhang, Lidong Yang, Chi Ian Vong, Kai Fung Chan, William K K Wu, Thomas N Y Kwong, Norman W S Lo, Margaret Ip, Sunny H Wong, Joseph J Y Sung, Philip W Y Chiu, Li Zhang
A rapid, direct, and low-cost method for detecting bacterial toxins associated with common gastrointestinal diseases remains a great challenge despite numerous studies and clinical assays. Motion-based detection through tracking the emerging micro- and nanorobots has shown great potential in chemo- and biosensing due to accelerated "chemistry on the move". Here, we described the use of fluorescent magnetic spore-based microrobots (FMSMs) as a highly efficient mobile sensing platform for the detection of toxins secreted by Clostridium difficile ( C...
January 2019: Science Advances
Tiffany Yang, Heather L Haas, Samir Patel, Ronald Smolowitz, Michael C James, Amanda S Williard
We documented blood biochemistry and haematology of healthy loggerhead turtles ( Caretta caretta ) in the Northwest (NW) Atlantic in order to establish clinical reference intervals (RIs) for this threatened population. Blood samples were analysed from migratory loggerheads captured off the Mid-Atlantic coast of the USA in 2011, 2012, 2013 and 2016 as part of a long-term research program. Blood variables were determined using a point-of-care analyser, and a veterinary diagnostic laboratory service. We calculated 95% RIs with associated 90% confidence intervals (CIs) for each blood variable...
2019: Conservation Physiology
Manuela Ferrario, Laura Brunelli, Fuhong Su, Antoine Herpain, Roberta Pastorelli
Septic shock is a medical emergency and is one of the main causes of mortality in critically ill patients. Given the pathophysiological complexity of sepsis spectrum and progression in clinical settings, animal models become essential tools to improve patient care, and to understand key mechanisms that may remain masked from the heterogeneity of clinical practice. Our aim was to verify whether the metabolic constellations we previously reported for septic shock patients appear also in our septic shock swine model as systemic markers of early disturbances in energy metabolism and hepatic homeostasis...
2019: Frontiers in Physiology
Sandeep Soni, Donald B Kohn
Gene modification of hematopoietic stem cells is increasingly becoming popular as a therapeutic approach, given the recent approvals and the number of new applications for clinical trials targeting monogenetic and immunodeficiency disorders. Technological advances in stem cell selection, culture, transduction and gene editing now allow for efficient ex vivo genetic manipulation of stem cells. Gene-addition techniques using viral vectors (mainly retrovirus- and lentivirus-based) and gene editing using various targeted nuclease platforms (e...
February 8, 2019: Cytotherapy
Veronika Rosecker, Christoph Denk, Melanie Maurer, Martin Wilkovitsch, Severin Mairinger, Thomas Wanek, Hannes Mikula
Radiotheranostics are designed by labeling targeting (bio)molecules with radionuclides for diagnostic or therapeutic application. As the pharmacokinetics of therapeutic compounds play a pivotal rule, chemically closely related imaging agents are used to evaluate the overall feasibility of the therapeutic approach. 'Theranostic relatives' utilizing different elements are frequently used in clinical practice. However, variations in pharmacokinetics, biodistribution, and target affinity due to the different chemical properties of the radioisotopes remain as hurdles to the design of optimized clinical tools...
February 11, 2019: Chembiochem: a European Journal of Chemical Biology
Tanja L Jensen, Maria K Kiersgaard, Lars F Mikkelsen, Dorte B Sørensen
Fasting of mice is a common procedure, which can affect the outcome of the study as well as animal welfare. In this study, we assess the effects of fasting, fasting duration and fasting initiation time in relation to light schedule and present suggestions for optimization of fasting. Male C57BL/6NCrl mice were fasted for 0, 3, 6, 12, 18 and 24 hours initiated either in the light period (photophase) or the dark period (scotophase). Body weight, gastric content, body temperature, corticosterone and 19 routine clinical chemistry parameters were evaluated...
February 10, 2019: Laboratory Animals
J S Pelletier, J Devine, K Capriotti, S B Barone, J A Capriotti
PURPOSE: To determine the ocular and systemic toxicity of a novel, topically applied ophthalmic gel preparation of povidone-iodine and dimethylsulfoxide in Dutch-Belted rabbits. MATERIALS AND METHODS: Rabbits were administered doses of the test material or control by ocular instillation four times/eye/day, seven days/week, for a minimum of 14 consecutive days. Dosing consisted of instillation of 50 µl of the appropriate test material solution or control material (saline) into each eye of the rabbit...
February 10, 2019: Cutaneous and Ocular Toxicology
Yang-Yang Sun, Xiao-Li Zhou, Wei Gao, Yu-Qing Cheng
OBJECTIVE: To investigate the clinicopathological and immunohistochemical features as well as diagnosis, differential diagnosis,treatment and prognosis of patients with primary renal non-hodgkin's lymphoma (PRNHL). METHODS: Clinical data of 2 patients with PRNHL from January 2013 to October 2017 were retrospectively analyzed. RESULTS: One of the two male patients PRNHL aged 51 years old, admitted to hospital for swelling and pain in the right waist, B-ultra sonic examination showed a very solid upper right renal space occupying lesion...
February 2019: Zhongguo Shi Yan Xue Ye Xue za Zhi
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