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Nadia Caccamo, Simone A Joosten, Tom H M Ottenhoff, Francesco Dieli
The induction of adaptive immunological memory, mediated by T and B cells, plays an important role in protective immunity to pathogens induced by previous infections or vaccination. Naive CD4+ T cells that have been primed by antigen develop into memory or effector cells, which may be distinguished by their capability to exert a long-term and rapid response upon re-challenge by antigen, to produce distinct cytokines and surface marker expression phenotypes such as CD45RA/RO, CD27, CD62L, and CCR7. Moreover, a distinct lineage of memory T cells populates tissues (tissue-resident memory T cells or TRM cells) which orchestratea the response to pathogens re encountered at tissue sites...
2018: Frontiers in Immunology
Andrea Arias, Pablo López, Raphael Sánchez, Yasuhiro Yamamura, Vanessa Rivera-Amill
The implementation of antiretroviral treatment combined with the monitoring of drug resistance mutations improves the quality of life of HIV-1 positive patients. The drug resistance mutation patterns and viral genotypes are currently analyzed by DNA sequencing of the virus in the plasma of patients. However, the virus compartmentalizes, and different T cell subsets may harbor distinct viral subsets. In this study, we compared the patterns of HIV distribution in cell-free (blood plasma) and cell-associated viruses (peripheral blood mononuclear cells, PBMCs) derived from ART-treated patients by using Sanger sequencing- and Next-Generation sequencing-based HIV assay...
August 9, 2018: International Journal of Environmental Research and Public Health
A Baragetti, G A Ramirez, M Magnoni, K Garlaschelli, L Grigore, M Berteotti, I Scotti, E Bozzolo, A Berti, P G Camici, A L Catapano, A A Manfredi, E Ammirati, G D Norata
BACKGROUND AND AIM: Patients with Systemic Lupus Erythematosus (SLE) present increased cardiovascular mortality compared to the general population. Few studies have assessed the long-term development and progression of carotid atherosclerotic plaque in SLE patients. Our aim was to investigate the association of clinical and laboratory markers of disease activity and classical cardiovascular risk factors (CVRF) with carotid atherosclerosis development in SLE patients in a prospective 5-year study...
January 2018: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
Elodie Picarda, Séverine Bézie, Laetitia Boucault, Elodie Autrusseau, Stéphanie Kilens, Dimitri Meistermann, Bernard Martinet, Véronique Daguin, Audrey Donnart, Eric Charpentier, Laurent David, Ignacio Anegon, Carole Guillonneau
Rat and human CD4+ and CD8+ Tregs expressing low levels of CD45RC have strong immunoregulatory properties. We describe here that human CD45 isoforms are nonredundant and identify distinct subsets of cells. We show that CD45RC is not expressed by CD4+ and CD8+ Foxp3+ Tregs, while CD45RA/RB/RO are. Transient administration of a monoclonal antibody (mAb) targeting CD45RC in a rat cardiac allotransplantation model induced transplant tolerance associated with inhibition of allogeneic humoral responses but maintained primary and memory responses against cognate antigens...
February 9, 2017: JCI Insight
Doureradjou Peroumal, Thiruvaimozhi Abimannan, Ravichandra Tagirasa, Jyothi Ranjan Parida, Santosh Kumar Singh, Prasantha Padhan, Satish Devadas
Activation Induced Cell Death of T helper cells is central to maintaining immune homeostasis and a perturbation often manifests in aberrant T helper cells that is associated with immunopathologies. Significant presence of T cells positive for IL-17A (Th17) and dual positive for IFN-γ/IL-17A (Th1/Th17) in both effector (CD45RA+RO+) and memory (CD45RA-RO+) compartments with differential FasL protein in RA peripheral blood suggested their differential TCR AICD sensitivity. Lowered active caspase-3 in Th17 and Th1/Th17 over Th1 cells confirmed their capability to resist AICD and pointed to early upstream events...
August 23, 2016: Oncotarget
Michelle L Joachims, Kerry M Leehan, Christina Lawrence, Richard C Pelikan, Jacen S Moore, Zijian Pan, Astrid Rasmussen, Lida Radfar, David M Lewis, Kiely M Grundahl, Jennifer A Kelly, Graham B Wiley, Mikhail Shugay, Dmitriy M Chudakov, Christopher J Lessard, Donald U Stone, R Hal Scofield, Courtney G Montgomery, Kathy L Sivils, Linda F Thompson, A Darise Farris
CD4(+) T cells predominate in salivary gland (SG) inflammatory lesions in Sjögren's syndrome (SS). However, their antigen specificity, degree of clonal expansion, and relationship to clinical disease features remain unknown. We used multiplex reverse-transcriptase PCR to amplify paired T cell receptor α (TCRα) and β transcripts of single CD4(+)CD45RA(-) T cells from SG and peripheral blood (PB) of 10 individuals with primary SS, 9 of whom shared the HLA DR3/DQ2 risk haplotype. TCRα and β sequences were obtained from a median of 91 SG and 107 PB cells per subject...
June 2, 2016: JCI Insight
Ewelina Krzywinska, Amelie Cornillon, Nerea Allende-Vega, Dang-Nghiem Vo, Celine Rene, Zhao-Yang Lu, Christine Pasero, Daniel Olive, Nathalie Fegueux, Patrick Ceballos, Yosr Hicheri, Michal Sobecki, Jean-François Rossi, Guillaume Cartron, Martin Villalba
The leucocyte-specific phosphatase CD45 is present in two main isoforms: the large CD45RA and the short CD45RO. We have recently shown that distinctive expression of these isoforms distinguishes natural killer (NK) populations. For example, co-expression of both isoforms identifies in vivo the anti tumor NK cells in hematological cancer patients. Here we show that low CD45 expression associates with less mature, CD56bright, NK cells. Most NK cells in healthy human donors are CD45RA+CD45RO-. The CD45RA-RO+ phenotype, CD45RO cells, is extremely uncommon in B or NK cells, in contrast to T cells...
2016: PloS One
Eveli Kallas, Kristi Huik, Silver Türk, Merit Pauskar, Ene-Ly Jõgeda, Marina Šunina, Tõnis Karki, Don Des Jarlais, Anneli Uusküla, Radko Avi, Irja Lutsar
Some individuals remain uninfected despite repeated exposure to HIV. This protection against HIV has been partly associated with altered T cell subset distributions and CCR5 expression levels. However, the majority of studies have been conducted in sexually exposed subjects. We aimed to assess whether HIV infection and intravenous drug use were associated with differences in CCR5 expression, immune activation on the CD4+ and CD8+ T cells and T cell distribution among Caucasian persons who inject drugs (PWIDs)...
June 2016: Medical Microbiology and Immunology
Ewelina Krzywinska, Nerea Allende-Vega, Amelie Cornillon, Dang-Nghiem Vo, Laure Cayrefourcq, Catherine Panabieres, Carlos Vilches, Julie Déchanet-Merville, Yosr Hicheri, Jean-François Rossi, Guillaume Cartron, Martin Villalba
Natural killer (NK) cells, a cytotoxic lymphocyte lineage, are able to kill tumor cells in vitro and in mouse models. However, whether these cells display an anti-tumor activity in cancer patients has not been demonstrated. Here we have addressed this issue in patients with several hematological cancers. We found a population of highly activated CD56(dim)CD16(+) NK cells that have recently degranulated, evidence of killing activity, and it is absent in healthy donors. A high percentage of these cells expressed natural killer cell p46-related protein (NKp46), natural-killer group 2, member D (NKG2D) and killer inhibitory receptors (KIRs) and a low percentage expressed NKG2A and CD94...
October 2015: EBioMedicine
L Mansi, E Daguindau, P Saas, F Pouthier, C Ferrand, A Dormoy, I Patry, F Garnache, P-S Rohrlich, E Deconinck, F Larosa
Hematopoietic cell transplantation (HCT) is a curative treatment for hematological malignancies. This therapeutic approach is associated with a profound immune deficiency and an increased rate of opportunistic infections. Nocardiosis is a rare bacterial infection occurring mainly in patients with deficient cell-mediated immunity, such as AIDS patients or transplant recipients. Diagnosis of nocardiosis can be challenging, as signs and symptoms are non-specific. Routine prophylaxis with trimethoprin/sulfamethoxazole (TMP/SMZ) does not prevent the risk of infection...
June 2014: Pathologie-biologie
Yi-Guang Chen, Susanne M Cabrera, Shuang Jia, Mary L Kaldunski, Joanna Kramer, Sami Cheong, Rhonda Geoffrey, Mark F Roethle, Jeffrey E Woodliff, Carla J Greenbaum, Xujing Wang, Martin J Hessner
Mechanisms associated with type 1 diabetes (T1D) development remain incompletely defined. Using a sensitive array-based bioassay where patient plasma is used to induce transcriptional responses in healthy leukocytes, we previously reported disease-specific, partially interleukin (IL)-1-dependent signatures associated with preonset and recent onset (RO) T1D relative to unrelated healthy control subjects (uHC). To better understand inherited susceptibility in T1D families, we conducted cross-sectional and longitudinal analyses of healthy autoantibody-negative (AA(-)) high HLA-risk siblings (HRS) (DR3 and/or DR4) and AA(-) low HLA-risk siblings (LRS) (non-DR3/non-DR4)...
November 2014: Diabetes
Li-Na Han, Xiao-Ming Lin, Jing Li, Chuan-Bo Zang, Li-Ming Yang, Guo-Lei Ding, Jin Peng
OBJECTIVE: Autoimmunity participates in chronic heart failure (CCI), it is CD4+ T lymphocytes that mainly induces myocardial infiltration and the progression of the disease. The purpose of this research is to assess changes of CD4+, CD8+ T lymphocyte subset, and phenotype of primary T cell (CD4+ CD45RA+) and memory T cells (CD4+ CD45RO+) in peripheral blood in aged male patients with CCI. And to investigate the immunomodulatory effects on subsets of CD4+, CD8+ and phenotype of CD4+ CD45RA+ and CD4+ CD45RO+ and the possible therapeutic mechanism...
September 2013: Chinese Journal of Applied Physiology
Nigel Klein, Delali Sefe, Ilaria Mosconi, Marisa Zanchetta, Hannah Castro, Marianne Jacobsen, Hannah Jones, Stefania Bernardi, Deenan Pillay, Carlo Giaquinto, A Sarah Walker, Diana M Gibb, Anita De Rossi
OBJECTIVES: To evaluate the immunological and viral consequences of planned treatment interruptions (PTI) in children with HIV. DESIGN: This was an immunological and virological sub-study of the Paediatric European Network for Treatment of AIDS (PENTA) 11 trial, which compared CD4-guided PTI of antiretroviral therapy (ART) with continuous therapy (CT) in children. METHODS: HIV-1 RNA and lymphocyte subsets, including CD4 and CD8 cells, were quantified on fresh samples collected during the study; CD45RA, CD45RO and CD31 subpopulations were evaluated in some centres...
2013: PloS One
Sudhir Kumar, Raza Ali Naqvi, Riyasat Ali, Richa Rani, Neena Khanna, D N Rao
Leprosy is a chronic human disease that results from infection of Mycobacterium leprae. T reg cells have been shown to have important implications in various diseases. However, in leprosy, it is still unclear whether T regs can mediate immune suppression during progression of the disease. In the present study, we have proposed the putative mechanism leading to high proportion of T reg cells and investigated its significance in human leprosy. High levels of TGF-β followed by adaptation of FoxP3(+) naive and memory (CD4(+)CD45RA(+)/RO(+)) T cells were observed as the principal underlying factors leading to higher generation of T reg cells during disease progression...
December 2013: Molecular Immunology
Pedro Castro, Montserrat Plana, Raquel González, Anna López, Anna Vilella, Jose M Nicolas, Teresa Gallart, Tomàs Pumarola, José M Bayas, José M Gatell, Felipe García
Presenting episodes of intermittent viremia (EIV) under combination antiretroviral therapy (cART) is frequent, but there exists some controversy about their consequences. They have been described as inducing changes in immune responses potentially associated with a better control of HIV infection. Conversely, it has been suggested that EIV increases the risk of virological failure. A retrospective analysis of a prospective, randomized double-blinded placebo-controlled study was performed. Twenty-six successfully treated HIV-infected adults were randomized to receive an immunization schedule or placebo, and after 1 year of follow-up cART was discontinued...
January 2013: AIDS Research and Human Retroviruses
N D'Vaz, Y Ma, J A Dunstan, T F Lee-Pullen, C Hii, S Meldrum, G Zhang, J Metcalfe, A Ferrante, S L Prescott
BACKGROUND: Previous studies have demonstrated that reduced T-cell protein kinase C zeta (PKCζ) expression is associated with allergy development in infants born to atopic mothers. This study examined whether this relationship extends to a general population and addressed the basis for the association. METHODS: A flow cytometry assay was developed for the measurement of T-cell PKCζ levels in PBMC, cord blood mononuclear cell and whole blood. Cord blood T-cell PKCζ levels were measured in 135 neonates, and allergic disease was evaluated by skin prick test and clinical examination at 12 months of age...
December 2012: Allergy
Katherine C Groves, David F Bibby, Duncan A Clark, Are Isaksen, Jane R Deayton, Jane Anderson, Chloe Orkin, Andrew J Stagg, Aine McKnight
BACKGROUND: The mechanism of CD4 T-cell decline in HIV-1 infection is unclear, but the association with plasma viral RNA load suggests viral replication is involved. Indeed, viremic controller patients with low viral RNA loads typically maintain high CD4 T-cell counts. Within a local cohort of 86 viremic controllers, we identify a subgroup (18 "discord controllers") with low CD4 T-cell counts that present clinical uncertainty. The underlying mechanism accounting for CD4 T-cell decline in the face of low or undetectable plasma (RNA) viral load remains unresolved...
December 1, 2012: Journal of Acquired Immune Deficiency Syndromes: JAIDS
Xiao-hui Wang, Yi-hua Xu, Lin Chen, Sheng Wei, Zheng-rong Yang, Xiang-dong Shi, Yan Zhang, Yong-xia Gan, Shao-fa Nie
OBJECTIVE: To explore the influence of T lymphocyte activation on HIV-1 susceptibility of Han Chinese. METHODS: In 2008, 37 HIV-1 highly exposed persistently seronegative individuals (ESNs) and 101 healthy controls were screened from Shenzhen. Flow cytometer was used to assay the expression difference of HIV-1 infection related co-receptor, the difference between the two groups were analyzed by Mann-Whitney U statistics methods. RESULTS: T cell HLA-DR(+) CD4 T cells and HLA-DR(+) expression of ESNs (12...
April 2012: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
Sajad A Dar, Shukla Das, Sambit N Bhattacharya, Vishnampettai G Ramachandran, Tanzeel Ahmed, Basu Dev Banerjee, Sidharth Sonthalia, Vikas Sood, Akhil C Banerjea
The diagnostic and pathological relevance of anti-desmoglein autoantibodies in common forms of pemphigus has been well established, and T cells have been shown to play a role in the onset and progression of these diseases. The role of superantigens in provoking polyclonal activation of T cells with many different fine specificities, possibly including autoreactive T cells and T-cell mediated autoantibody response, is unknown. Further, abnormal T-cell function may lead to opportunistic infections particularly with Candida...
October 2011: Journal of Dermatology
S Gurkan, Y Luan, N Dhillon, S R Allam, T Montague, J S Bromberg, S Ames, S Lerner, Z Ebcioglu, V Nair, R Dinavahi, V Sehgal, P Heeger, B Schroppel, B Murphy
Depletional induction therapies are routinely used to prevent acute rejection and improve transplant outcome. The effects of depleting agents on T-cell subsets and subsequent T-cell reconstitution are incompletely defined. We used flow cytometry to examine the effects of rabbit antithymocyte globulin (rATG) on the peripheral T-cell repertoire of pediatric and adult renal transplant recipients. We found that while rATG effectively depleted CD45RA+CD27+ naïve and CD45RO+CD27+ central memory CD4+ T cells, it had little effect on CD45RO+CD27- CD4+ effector memory or CD45RA+CD31-, CD45RO+CD27+ and CD45RO+CD27- CD8+ T cell subsets...
September 2010: American Journal of Transplantation
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