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Der f AND der p AND allergen

P W Hellings, B Pugin, G Mariën, C Bachert, C Breynaert, D M Bullens, J L Ceuppens, G Clement, T Cox, D Ebo, P Gevaert, S Halewyck, V Hox, K Ladha, R Jacobs, P Rombaux, R Schrijvers, K Speleman, X Van der Brempt, L Van Gerven, O Vanderveken, B Verhaeghe, K Vierstraete, S Vlaminck, J-B Watelet, J Bousquet, S F Seys
Allergic rhinitis (AR) affects 23-30% of the European population with equal prevalence reported in Belgium. Despite guidelines on the correct use of effective treatment, up to 40% of AR patients remain uncontrolled. Allergen immunotherapy (AIT) has been shown to improve the level of control up to 84% of patients being controlled by AIT. Recently, new guidelines for AIT have been published, supporting the clinical evidence for effectiveness of various subcutaneous and sublingual products for AIT in patients who are allergic to airborne allergens...
2019: Clinical and Translational Allergy
Kavita Reginald, Fook Tim Chew
Der p 2 is a major dust mite allergen and >80% of mite allergic individuals have specific IgE to this allergen. Although it is well characterized in terms of allergenicity, there is still some ambiguity in terms of its biological function. Three-dimensional structural analysis of Der p 2 and its close homologues indicate the presence of a hydrophobic cavity which can potentially bind to lipid molecules. In this study, we aimed to identify the potential ligand of Der p 2. Using a liposome pulldown assay, we show that recombinant Der p 2 binds to liposomes prepared with exogenous cholesterol in a dose dependent fashion...
February 7, 2019: Scientific Reports
Dong Il Won, Sujeong Kim, Eun Hee Lee
Background: IgG-mediated anaphylaxis occurs after infusion of certain monoclonal antibody-based therapeutics. New in vitro tests are urgently needed to diagnose such reactions. We investigated whether allergens trigger neutrophil oxidative burst (OB) and if neutrophil OB occurs due to allergen-specific IgG (sIgG). Methods: Neutrophil OB was measured by dihydrorhodamine 123 flow cytometry using a leukocyte suspension spiked with a very small patch of the allergen crude extract, Dermatophagoides farinae ( Der f )...
December 2018: Blood Research
Chang-Ching Yeh, Keh-Gong Wu, Peng-Hui Wang
There is an increase in prevalence and financial burden of childhood atopic disorders in recent years. Understanding allergic conditions of pregnant women is important for developing strategies for prevention and management of allergy-related diseases. However, little is currently known about the atopic conditions in pregnant women.The sera from 46 pregnant women were analyzed for allergen-specific IgE antibodies using the Optigen assay and SDS-PAGE immunoblot analysis.Results from the Optigen assay showed that 20 (43%) of the 46 serum samples analyzed demonstrated IgE reactivity against mite p (Dermatophagoides pteronyssinus) (95%), mite f (D farinae) (95%), house dust (60%), cat (25%), shrimp (20%), crab (15%), cockroach (10%), dog (5%), latex (5%), willow black (5%), and timothy grass (5%)...
December 2018: Medicine (Baltimore)
Jianglong Peng, Hao Yin, Ying Zhou, Haoyuan Jia, Yubao Cui
BACKGROUND: The dust mite Dermatophagoides farinae is a common worldwide cause of indoor allergies induced by mite proteins, including the mid-tier allergen Der f 7. Objective To identify conformational epitopes in Der f 7 using mimotope mapping and computational modelling. METHODS: Here, we used standard hybridoma technology to generate 3 new monoclonal antibodies against Der f 7 and performed mimotope mapping by probing a random peptide phage display library. Computational tools, including Minox and the DiscoTope-2...
December 8, 2018: Protein and Peptide Letters
Shanna Bastiaan-Net, Marit Reitsma, Jan H G Cordewener, Johanna P M van der Valk, Twan A H P America, Anthony E J Dubois, Roy Gerth van Wijk, Huub F J Savelkoul, Nicolette W de Jong, Harry J Wichers
BACKGROUND: Allergic sensitisation towards cashew nut often happens without a clear history of eating cashew nut. IgE cross-reactivity between cashew and pistachio nut is well described; however, the ability of cashew nut-specific IgE to cross-react to common tree nut species and other Anacardiaceae, like mango, pink peppercorn, or sumac is largely unknown. OBJECTIVES: Cashew nut allergic individuals may cross-react to foods that are phylogenetically related to cashew...
2019: International Archives of Allergy and Immunology
Selçuk Yazıcı, Soner Güneş, Melike Kurtuluş-Çokboz, Özlem Kemer, Gülce Baranlı, Sezin Aşık-Akman, Demet Can
Yazıcı S, Güneş S, Kurtuluş-Çokboz M, Kemer Ö, Baranlı G, Aşık-Akman S, Can D. Allergen variability and house dust mite sensitivity in pre-school children with allergic complaints. Turk J Pediatr 2018; 60: 41-49. The increase in the prevalence of allergic diseases in pre-school children who are often at home may be due to an increase in house dust mite sensitivity, which is rarely expected in this age group. In our study, it was aimed to investigate allergen sensitivities, especially house dust mite sensitivity in pre-school children with allergic disease complaints by skin prick test (SPT)...
2018: Turkish Journal of Pediatrics
Yongshi Yang, Rongfei Zhu, Nan Huang, Wenjing Li, Wei Zhang, Yin Wang, Lin Yang
Background House dust mites (HDMs) are the major aeroallergens in patients with rhinitis and/or asthma in China. However, the molecular sensitization of HDM is varied in different regions. Objective To investigate the Dermatophagoides pteronyssinus ( Der p) molecular sensitization profile of allergic rhinitis (AR) patients in Central China. Methods AR patients with positive skin prick tests to Der p were enrolled in our study. Specific immunoglobulin E (sIgE) for Der p, Dermatophagoides farinae ( Der f), Acarus siro ( Aca s), Blomia tropicalis ( Blo t), and Der p components Der p1, Der p2, and Der p10 were measured in all patients...
January 1, 2018: American Journal of Rhinology & Allergy
Jose F Cantillo, Leonardo Puerta, Enrique Fernandez-Caldas, Jose L Subiza, Irene Soria, Stefan Wöhrl, Christof Ebner, Walter Keller, Yvonne Resch-Marat, Susanne Vrtala, Barbara Bohle
BACKGROUND: Aedes aegypti and Dermatophagoides pteronyssinus contain important allergens including cross-reactive tropomyosins. However, the functional and clinical relevance of their cross-reactivity is still debated. OBJECTIVE: To analyse the humoral and cellular cross-reactivity of recombinant Aed a 10.01, Aed a 10.02 and Der p 10. METHODS: Sera from 15 Austrian house dust mite-allergic, Der p 10-sensitized individuals were tested for IgE reactivity to recombinant tropomyosins in ELISA, inhibition ELISA and basophil activation tests...
October 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
Suriani Alimuddin, Iris Rengganis, Cleopas M Rumende, Siti Setiati
BACKGROUND: nowadays, specific IgE measurement has been conducted in Indonesia, however there is still lack of data regarding diagnostic test to detect inhalant allergen in patients with respiratory allergies.This study aimed to determine the accuracy of specific IgE test in diagnosing specific sensitization of inhalant allergen in patients with respiratory allergies. METHODS: this was a cross sectional study in patients with respiratory allergies and part of epidemiology study regarding to specific IgE sensitization in Allergy-Immunollogy Division, Cipto Mangunkusumo Hospital, Jakarta within November-December 2016...
April 2018: Acta Medica Indonesiana
Nina Fischer, Noémi Tarpataki, Federica Leidi, Ana Rostaher, Claude Favrot
BACKGROUND: Previously published studies evaluating a recombinant Der f 2-based immunotherapy have demonstrated efficacy in the treatment of dogs experimentally and naturally sensitized to house dust mites (HDM). Der f 2 sensitization is thought to play a minor role in European atopic dogs sensitized to HDM. OBJECTIVE: The study evaluated the short-term efficacy of a recombinant Der f 2 product in the treatment of naturally sensitized atopic dogs in Switzerland and Hungary...
June 17, 2018: Veterinary Dermatology
Petr Panzner, Martina Vachová, Tomáš Vlas, Petra Vítovcová, Petra Brodská, Marek Malý
Background: The major sources of allergens in the indoor air include house dust mites, dander derived from domestic animals and rodents, cockroach, and several fungi. Mites are the main cause of allergies in some countries with a warmer climate, but the epidemiological significance of mite and cockroach allergens in Central Europe has not been established yet. Methods: We assessed sensitization profiles of allergy patients in a Central European region in regard to sensitization to mites and cockroach...
2018: Clinical and Translational Allergy
Passara Jongkhajornpong, Wannada Laisuan
Purpose: We report a case of refractory atopic keratoconjunctivitis (AKC) which was successfully treated with subcutaneous immunotherapy (SCIT). Case Report: A 22-year-old woman presented with severe allergic conjunctivitis for one and a half year. She failed to respond to conventional topical anti-allergic medications, topical corticosteroid, as well as topical cyclosporine A. Therefore, oral corticosteroids had to be prescribed to control the exacerbation for 1 year...
September 2017: Case Reports in Ophthalmology
M Sánchez-Borges, L González-Aveledo, A Capriles-Hulett, F Caballero-Fonseca
Scabies is observed with relatively high frequency in Allergy and Dermatology clinics in developing countries where poor sanitary conditions are prevalent and increasingly in some areas of the world with increased immigrant populations. Since the immunological response to scabies mites includes the production of IgE class antibodies to Sarcoptes scabiei allergens which cross-react with Dermatophagoides major allergens Der p 1 and Der p 2, positive immediate-type skin tests to house dust mite extracts should be interpreted cautiously...
May 2018: Allergologia et Immunopathologia
Päivi M Salo, Jesse Wilkerson, Kathryn M Rose, Richard D Cohn, Agustin Calatroni, Herman E Mitchell, Michelle L Sever, Peter J Gergen, Peter S Thorne, Darryl C Zeldin
BACKGROUND: Bedroom allergen exposures contribute to allergic disease morbidity because people spend considerable time in bedrooms, where they come into close contact with allergen reservoirs. OBJECTIVE: We investigated participant and housing characteristics, including sociodemographic, regional, and climatic factors, associated with bedroom allergen exposures in a nationally representative sample of the US population. METHODS: Data were obtained from National Health and Nutrition Examination Survey 2005-2006...
May 2018: Journal of Allergy and Clinical Immunology
T Erban, R Klubal
BACKGROUND: Optimised purification steps for concentrating trace target native antigens are needed. Combining the p-aminobenzamidine ligand with protease inactivation enables partial purification of mite non-protease allergens lacking proteases. OBJECTIVE: We sought to analyse in detail proteins obtained using this method from eight species of synanthropic acaridid mites and tested IgE reactivity using pooled human sera. MATERIALS AND METHODS: Proteins affinity bound to p-aminobenzamidine as a ligand were identified by MALDI TOF/TOF...
May 2018: Allergologia et Immunopathologia
Katsuyo Ohashi-Doi, Hirokazu Kito, Weibin Du, Hiroshi Nakazawa, Henrik Ipsen, Pernille Gudmann, Kaare Lund
BACKGROUND: In sublingual immunotherapy (SLIT), the immune system is addressed by solubilized allergen that interacts with immunocompetent cells of the oral mucosa, the efficiency of which is governed by 2 main factors of SLIT allergen bioavailability: the allergen concentration and the mucosal contact time. Recently, 3 house dust mite (HDM) SLIT tablets were developed that differ with regard to allergen content, nominal strength (maintenance doses: 6 SQ-HDM/10,000 Japanese Allergen Units [JAU], 12 SQ-HDM/ 20,000 JAU, and 300 IR/57,000 JAU), and formulation (freeze-dried/compressed)...
2017: International Archives of Allergy and Immunology
Krisztina Szegedi, Amanda van Lier, Pieter C Res, Saskia Chielie, Jan D Bos, Sanja Kezic, Maritza A Middelkamp-Hup, Rosalie M Luiten
Aero-allergens, such as house dust mite (HDM), have been suggested to play a role in the initiation of atopic dermatitis (AD)-related skin inflammation. Here, we analysed the proliferation and the cytokine expression of blood-derived T cells from AD and healthy individuals upon HDM-allergen stimulation. The proliferating cells from healthy individuals and AD patients had a significantly different, distinct cytokine profile: in AD blood, we found increased frequencies of HDM-reactive IL-31-producing T cells, as well as a decreased Th1/Th2 and Tc1/Tc2 ratio, suggesting that allergen-specific T cells in blood of chronic AD patients are subject to pre-existent Th2-Tc2 and "Th31-Tc31" programming...
April 2018: Experimental Dermatology
D Betty Lew, Christie F Michael, Tracie Overbeck, W Scout Robinson, Erin L Rohman, Jeffrey M Lehman, Jennifer K Patel, Brandi Eiseman, Kim S LeMessurier, Amali E Samarasinghe, M Waleed Gaber
One of the unmet needs for asthma management is a new therapeutic agent with both anti-inflammatory and anti-smooth muscle (ASM) remodeling effects. The mannose receptor (MR) family plays an important role in allergen uptake and processing of major allergens Der p 1 and Fel d 1. We have previously reported that ASM cells express a mannose receptor (ASM-MR) and that mannan derived from Saccharomyces cerevisiae (SC-MN) inhibits mannosyl-rich lysosomal hydrolase-induced bovine ASM cell proliferation. Using a humanized transgenic mouse strain (huASM-MRC2) expressing the human MRC2 receptor in a SM tissue-specific manner, we have demonstrated that ASM hyperplasia/hypertrophy can occur as early as 15 days after allergen challenge in this mouse model and this phenomenon is preventable with SC-MN treatment...
2017: Journal of Immunology Research
Kouji Kawano, Takuya Mizuno
BACKGROUND: Dermatophagoides farinae is a major and common environmental house dust mite involved in canine atopic dermatitis (CAD). A formulation of recombinant protein Der f 2 is available in Japan for immunotherapy in dogs. HYPOTHESIS/OBJECTIVES: To evaluate the efficacy of a recombinant allergen based on Der f 2-pullulan for the treatment of cAD. METHODS: Dogs (n = 15) with atopic dermatitis were administered Der f 2 conjugated with pullulan (-P)...
December 2017: Veterinary Dermatology
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