keyword
https://read.qxmd.com/read/34185416/dual-role-of-the-mir-146-family-in-rhinovirus-induced-airway-inflammation-and-allergic-asthma-exacerbation
#21
JOURNAL ARTICLE
Anet Laanesoo, Egon Urgard, Kapilraj Periyasamy, Martti Laan, Yury A Bochkov, Alar Aab, Nathaniel Magilnick, Margus Pooga, James E Gern, Sebastian L Johnston, Jonathan M Coquet, Mark P Boldin, Jesper Wengel, Alan Altraja, Grazyna Bochenek, Bogdan Jakiela, Ana Rebane
Rhinovirus (RV) infections are associated with asthma exacerbations. MicroRNA-146a and microRNA-146b (miR-146a/b) are anti-inflammatory miRNAs that suppress signaling through the nuclear factor kappa B (NF-κB) pathway and inhibit pro-inflammatory chemokine production in primary human bronchial epithelial cells (HBECs). In the current study, we aimed to explore whether miR-146a/b could regulate cellular responses to RVs in HBECs and airways during RV-induced asthma exacerbation. We demonstrated that expression of miR-146a/b and pro-inflammatory chemokines was increased in HBECs and mouse airways during RV infection...
June 2021: Clinical and Translational Medicine
https://read.qxmd.com/read/34182079/distribution-of-mite-family-monkey-king-in-rapeseed-brassica-napus-l-and-its-influence-on-gene-expression
#22
JOURNAL ARTICLE
Jinna Hou, Dandan Lu, Annaliese S Mason, Baoquan Li, Sufang An, Gaoyuan Li, Dongfang Cai
Miniature inverted-repeat transposable elements (MITEs) are a group of class II transposable elements. The MITE Monkey King (MK) was first discovered upstream of BnFLC.A10. In this study, genome resequencing of four selected B. napus accessions, revealed more than 4000 distributed copies of MKs constituting ~2.4 Mb of the B. napus genomic sequence and caused 677 polymorphisms among the four accessions. MK -polymorphism-related markers across 128 natural and 58 synthetic accessions revealed more polymorphic MK s in natural than synthetic accessions...
June 25, 2021: Genomics
https://read.qxmd.com/read/34044154/genome-wide-identification-of-mirnas-and-target-regulatory-network-in-the-invasive-ectoparasitic-mite-varroa-destructor
#23
JOURNAL ARTICLE
Paula L C Fonseca, Jonathan J Mucherino M, Joel A M Porto, Juliana N Armache, João Paulo P de Almeida, Felipe da Silva, Roenick P Olmo, Isaque João da Silva Faria, Daniel Santana de Carvalho, Aristóteles Góes-Neto, Ronan Xavier Corrêa, Carlos P Pirovani, Luis G C Pacheco, Marco Antônio Costa, Eric R G R Aguiar
Varroa destructor is an ectoparasite mite that attacks bees leading to colony disorders worldwide. microRNAs (miRNAs) are key molecules used by eukaryotes to post-transcriptional control of gene expression. Nevertheless, still lack information about V. destructor miRNAs and its regulatory networks. Here, we used an integrative strategy to characterize the miRNAs in the Varroa mite. We identified 310 precursors that give rise to 500 mature miRNAs, which 257 are likely mite-specific elements. miRNAs showed canonical length ranging between 18 and 25 nucleotides and 5' uracil preference...
May 24, 2021: Genomics
https://read.qxmd.com/read/33852061/molecular-hydrogen-alleviates-asthma-through-inhibiting-il-33-ilc2-axis
#24
JOURNAL ARTICLE
Jingxi Zhang, Xiumin Feng, Yunxin Fan, Guanglin Zhu, Chong Bai
BACKGROUND: Asthma is one of the most common noninfectious chronic diseases characterized by type II inflammation. This study aimed to investigate the effects of molecular hydrogen on the pathogenesis of asthma. METHODS: OVA sensitized asthma mouse model and house dust mite treated 16HBE cellular model were established and hydrogen/oxygen mixture was used to treat asthmatic mice and 16HBE cells. Serum and BALF cytokines were measured with specific ELISA assays. E-cadherin and ZO-1 were detected by immunohistochemical staining and expression of caspase 3 and 9, NF-κB, IL-33 and ST2 was assessed by quantitative real-time PCR, western blot and/or immunofluorescence...
May 2021: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
https://read.qxmd.com/read/33846533/inhibition-of-mir-99a-5p-prevents-allergen-driven-airway-exacerbations-without-compromising-type-2-memory-responses-in-the-intestine-following-helminth-infection
#25
JOURNAL ARTICLE
Lewis Entwistle, Helena Aegerter, Stephanie Czieso, Eleni Amaniti, Riccardo Guidi, Abdul Sesay, Nikolay Nikolov, Probir Chakravaty, Alison Huynh, Jessica Mills, Sean Flanagan, Shannon Hambro, Victor Nunez, Yi Cao, Christine Clarke, Angela Martzall, Laurie Leong, Dennis Wilson, Cary Austin, Mark Wilson
Acute exacerbations (AE) of asthma, remain one of the biggest concerns for patients living with asthma. As such, identifying the causes, the molecular mechanisms involved and new therapeutic interventions to prevent AE is a high priority. Immunity to intestinal helminths involves the reactivation of type-2 immune responses leading to smooth muscle contraction and mucus hypersecretion-physiological processes very similar to acute exacerbations in the airways following allergen exposure. In this study, we employed a murine model of intestinal helminth infection, using Heligmosomoides polygyrus, to identify miRNAs during active expulsion, as a system for the identification of miRNAs that may contribute to AE in the airways...
April 12, 2021: Mucosal Immunology
https://read.qxmd.com/read/33780108/a-novel-transposable-element-derived-microrna-participates-in-plant-immunity-to-rice-blast-disease
#26
JOURNAL ARTICLE
Sonia Campo, Ferran Sánchez-Sanuy, Rosany Camargo-Ramírez, Jorge Gómez-Ariza, Patricia Baldrich, Lidia Campos-Soriano, Mauricio Soto-Suárez, Blanca San Segundo
MicroRNAs (miRNAs) are small non-coding RNAs that direct post-transcriptional gene silencing in plant development and stress responses through cleavage or translational repression of target mRNAs. Here, we report the identification and functional characterization of a new member of the miR812 family in rice (named as miR812w) involved in disease resistance. miR812w is present in cultivated Oryza species, both japonica and indica subspecies, and wild rice species within the Oryza genus, but not in dicotyledonous species...
March 29, 2021: Plant Biotechnology Journal
https://read.qxmd.com/read/33461614/rnai-gene-knockdown-in-the-poultry-red-mite-dermanyssus-gallinae-de-geer-1778-a-tool-for-functional-genomics
#27
JOURNAL ARTICLE
Wan Chen, Kathryn Bartley, Francesca Nunn, Alan S Bowman, Jeremy M Sternberg, Stewart T G Burgess, Alasdair J Nisbet, Daniel R G Price
BACKGROUND: The avian haematophagous ectoparasite Dermanyssus gallinae, commonly known as the poultry red mite, causes significant economic losses to the egg-laying industry worldwide and also represents a significant welfare threat. Current acaricide-based controls are unsustainable due to the mite's ability to rapidly develop resistance, thus developing a novel sustainable means of control for D. gallinae is a priority. RNA interference (RNAi)-mediated gene silencing is a valuable tool for studying gene function in non-model organisms, but is also emerging as a novel tool for parasite control...
January 18, 2021: Parasites & Vectors
https://read.qxmd.com/read/33452589/investigation-of-circular-rnas-in-an-ectoparasitic-mite-varroa-destructor-acarina-varroidae-of-the-honey-bee
#28
JOURNAL ARTICLE
Zheguang Lin, Hao Xu, Xiaoling Su, Yalu Ke, Wei Wang, Yujiao Li, Mingliang Zhuang, Heng Chen, Yibing Liu, Kang Wang, Guohong Chen, Ting Ji
Circular RNAs (circRNAs) are a large class of non-protein-coding transcripts that are involved in a diverse spectrum of regulatory mechanisms across a broad range of biological processes. To date, however, few studies on circRNAs have investigated their role in the biology of invertebrate parasites. The ectoparasitic mite Varroa destructor is perceived as the principal biotic threat towards global honey bee health. This parasite cannot be sustainably controlled partially due to the lack of knowledge about its basic molecular biology...
January 16, 2021: Parasitology Research
https://read.qxmd.com/read/33173985/differentially-expressed-circular-rnas-in-a-murine-asthma-model
#29
JOURNAL ARTICLE
Hui Bao, Qiuyan Zhou, Qiuju Li, Mengmeng Niu, Sanfeng Chen, Pingchang Yang, Zhigang Liu, Lixin Xia
Allergic asthma is one of the most common allergic diseases; however, the mechanisms underlying its development have yet to be fully elucidated. Although allergic diseases are inheritable, genetic variance alone cannot explain the notable increase in the prevalence of allergic diseases over a short period of time in recent decades. Recently, research focus has been shifting to epigenetic factors, such as non‑coding RNAs. Circular RNAs (circRNAs) are involved in the pathogenesis of various diseases. The aim of the present study was to further elucidate the etiology of allergic asthma by analyzing aberrantly expressed circRNAs in a murine asthma model...
December 2020: Molecular Medicine Reports
https://read.qxmd.com/read/32887419/allergic-inflammation-alters-microrna-expression-profile-in-adipose-tissue-in-the-rat
#30
JOURNAL ARTICLE
Dawid Szczepankiewicz, Wojciech Langwiński, Paweł Kołodziejski, Ewa Pruszyńska-Oszmałek, Maciej Sassek, Joanna Nowakowska, Agata Chmurzyńska, Krzysztof W Nowak, Aleksandra Szczepankiewicz
Adipose tissue is a major source of circulating exosomal microRNAs (miRNAs) that are modulators of the immune response in various types of tissues and organs, including airways. Still, no evidence exists if allergic airway inflammation may affect fat tissue inflammation via alterations in the miRNA expression profile. Therefore, we investigated the miRNA expression profile in the adipose tissue upon induced allergic inflammation in the airways in the rat. Brown Norway rats were chronically sensitized to house dust mite extract for seven weeks...
September 2, 2020: Genes
https://read.qxmd.com/read/32202463/expression-of-rice-sir109944-in-arabidopsis-affects-plant-immunity-to-multiple-fungal-pathogens
#31
JOURNAL ARTICLE
Lulu Qiao, Hongwei Zhao, Hailing Jin, Dongdong Niu
Plant small RNA (sRNA)-mediated gene expression has a conserved role in regulating plant growth, development, and immunity. Heterologous expression of sRNA contributes to determining whether the function of sRNA is conservative or independent. We recently characterized the Tourist-miniature inverted-repeat transposable element (MITE)-derived siR109944 had a conserved function that enhanced susceptibility to Rhizoctonia solani infection by affecting auxin homeostasis in rice and Arabidopsis . To ascertain whether the function of rice siR109944 has a broad-spectrum immunity in Arabidopsis , we infected Arabidopsis with a variety of fungal pathogens...
March 23, 2020: Plant Signaling & Behavior
https://read.qxmd.com/read/32044723/nanoparticle-delivery-of-anti-inflammatory-lna-oligonucleotides-prevents-airway-inflammation-in-a-hdm-model-of-asthma
#32
JOURNAL ARTICLE
Sabrina C Ramelli, Brian S Comer, Jared M McLendon, Lydia L Sandy, Andrew P Ferretti, Robert Barrington, Jeff Sparks, Majed Matar, Jason Fewell, William T Gerthoffer
To address the problem of poor asthma control due to drug resistance, an antisense oligonucleotide complementary to mmu-miR-145a-5p (antimiR-145) was tested in a house dust mite mouse model of mild/moderate asthma. miR-145 was targeted to reduce inflammation, regulate epithelial-mesenchymal transitions, and promote differentiation of structural cells. In addition, several chemical variations of a nontargeting oligonucleotide were tested to define sequence-dependent effects of the miRNA antagonist. After intravenous administration, oligonucleotides complexed with a pegylated cationic lipid nanoparticle distributed to most cells in the lung parenchyma but were not present in smooth muscle or the mucosal epithelium of the upper airways...
January 14, 2020: Molecular Therapy. Nucleic Acids
https://read.qxmd.com/read/31730193/domestication-of-high-copy-transposons-underlays-the-wheat-small-rna-response-to-an-obligate-pathogen
#33
JOURNAL ARTICLE
Manuel Poretti, Coraline Praz, Lukas Meile, Carol Kälin, Luisa Katharina Schaefer, Michael Schläfli, Victoria Widrig, Andrea Sanchez Vallet, Thomas Wicker, Salim Bourras
Plant genomes have evolved several evolutionary mechanisms to tolerate and make use of transposable elements (TEs). Of these, transposon domestication into cis-regulatory and microRNA (miRNA) sequences is proposed to contribute to abiotic/biotic stress adaptation in plants. The wheat genome is derived at 85% from TEs, and contains thousands of miniature inverted-repeat transposable elements (MITEs), whose sequences are particularly prone for domestication into miRNA precursors. In this study, we investigate the contribution of TEs to the wheat small RNA immune response to the lineage-specific, obligate powdery mildew pathogen...
November 15, 2019: Molecular Biology and Evolution
https://read.qxmd.com/read/31221987/multi-method-molecular-characterisation-of-human-dust-mite-associated-allergic-asthma
#34
JOURNAL ARTICLE
E Whittle, M O Leonard, T W Gant, D P Tonge
Asthma is a chronic inflammatory disorder of the airways. Disease presentation varies greatly in terms of cause, development, severity, and response to medication, and thus the condition has been subdivided into a number of asthma phenotypes. There is still an unmet need for the identification of phenotype-specific markers and accompanying molecular tools that facilitate the classification of asthma phenotype. To this end, we utilised a range of molecular tools to characterise a well-defined group of female adults with poorly controlled atopic asthma associated with house dust mite (HDM) allergy, relative to non-asthmatic control subjects...
June 20, 2019: Scientific Reports
https://read.qxmd.com/read/30870071/de-novo-origination-of-mirnas-through-generation-of-short-inverted-repeats-in-target-genes
#35
JOURNAL ARTICLE
Shanfa Lu
MIRNA (MIR) gene origin and early evolutionary processes, such as hairpin precursor sequence origination, promoter activity acquirement and the sequence of these two processes, are fundamental and fascinating subjects. Three models, including inverted gene duplication, spontaneous evolution and transposon transposition, have been proposed for de novo origination of hairpin precursor sequence. However, these models still open to discussion. In addition, de novo origination of MIR gene promoters has not been well investigated...
March 14, 2019: RNA Biology
https://read.qxmd.com/read/30648010/a-miniature-inverted-repeat-transposable-element-addin-mite-located-inside-a-wd40-gene-is-conserved-in-andropogoneae-grasses
#36
JOURNAL ARTICLE
Clicia Grativol, Flavia Thiebaut, Sara Sangi, Patricia Montessoro, Walaci da Silva Santos, Adriana S Hemerly, Paulo C G Ferreira
Miniature inverted-repeat transposable elements (MITEs) have been associated with genic regions in plant genomes and may play important roles in the regulation of nearby genes via recruitment of small RNAs (sRNA) to the MITEs loci. We identified eight families of MITEs in the sugarcane genome assembly with MITE-Hunter pipeline. These sequences were found to be upstream, downstream or inserted into 67 genic regions in the genome. The position of the most abundant MITE (Stowaway-like) in genic regions, which we call AddIn-MITE, was confirmed in a WD40 gene...
2019: PeerJ
https://read.qxmd.com/read/30406061/microrna-27b-3p-modulates-syk-in-pediatric-asthma-induced-by-dust-mites
#37
JOURNAL ARTICLE
Xiaoyan Dong, Nanbert Zhong, Yudan Fang, Qin Cai, Min Lu, Quan Lu
The PI3K-AKT pathway is known to regulate cytokines in dust mite-induced pediatric asthma. However, the underlying molecular steps involved are not clear. In order to clarify further the molecular steps, this study investigated the expression of certain genes and the involvement of miRNAs in the PI3K-AKT pathway, which might affect the resultant cytokine-secretion. in-vivo and in-vitro ELISA, qRT-PCR and microarrays analyses were used in this study. A down-expression of miRNA-27b-3p in dust mite induced asthma group (group D) was found by microarray analysis...
2018: Frontiers in Pediatrics
https://read.qxmd.com/read/30245919/effects-of-allergic-stimulation-and-glucocorticoids-on-mir-155-in-cd4-t-cells
#38
JOURNAL ARTICLE
Elizabeth Daniel, Alanna Roff, Man-Hsun Hsu, Ronaldo Panganiban, Kristin Lambert, Faoud Ishmael
RATIONALE: MicroRNAs (miRNAs) are emerging as important regulators of allergic inflammation and potential therapeutic targets. We sought to identify which miRNAs are expressed in CD4+ T-cells and determine whether allergic stimuli or glucocorticoids alter their expression. METHODS: After IRB approval, blood was collected from dust mite (DM) allergic rhinitis subjects (n=20), non-allergic controls (n=8), and asthmatics (n=16). Peripheral blood mononuclear cells were incubated with dust mite extract (DME), diluent control, or DME + dexamethasone (0...
2018: American Journal of Clinical and Experimental Immunology
https://read.qxmd.com/read/29644894/decreased-epithelial-and-sputum-mir-221-3p-associates-with-airway-eosinophilic-inflammation-and-cxcl17-expression-in-asthma
#39
JOURNAL ARTICLE
Kan Zhang, Yuxia Liang, Yuchen Feng, Wenliang Wu, Huilan Zhang, Jianguo He, Qinghua Hu, Jianping Zhao, Yongjian Xu, Zheng Liu, Guohua Zhen
Airway eosinophilic inflammation is a key feature of type 2 high asthma. The role of epithelial microRNA (miR) in airway eosinophilic inflammation remains unclear. We examined the expression of miR-221-3p in bronchial brushings, induced sputum, and plasma from 77 symptomatic, recently diagnosed, steroid-naive subjects with asthma and 36 healthy controls by quantitative PCR and analyzed the correlation between miR-221-3p expression and airway eosinophilia. We found that epithelial, sputum, and plasma miR-221-3p expression was significantly decreased in subjects with asthma...
August 1, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://read.qxmd.com/read/29424082/a-microrna-1-gene-tci-mir-1-3p-is-involved-in-cyflumetofen-resistance-by-targeting-a-glutathione-s-transferase-gene-tcgstm4-in-tetranychus-cinnabarinus
#40
JOURNAL ARTICLE
Y Zhang, K Feng, J Hu, L Shi, P Wei, Z Xu, G Shen, M Li, Q Xu, L He
microRNA-1 (miR-1) is a well-studied conservative microRNA (miRNA) involved in immune responses in mammals and insects. However, little is known about its role in pesticide resistance in arthropods. In this study, we found that a microRNA belong to miR-1 family (tci-miR-1-3p) was significantly down-regulated in a cyflumetofen-resistant strain (CYR) of Tetranychus cinnabarinus compared with its homologous susceptible strain (SS), indicating an involvement of miR-1 in cyflumetofen resistance in mites. One glutathione S-transferase (GST) gene (TCGSTM4, a mu class GST gene), a candidate target gene of tci-miR-1-3p, was found to be significantly down-regulated when tci-miR-1-3p was over-expressed...
June 2018: Insect Molecular Biology
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