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Yonca Yuzugullu Karakus, Semih Isik
Aqueous two-phase partitioning system (ATPS) was used to extract and purify catalase from Bacillus pumilus. The system parameters for effective purification of catalase were optimized. The best catalase recovery (123%) with a 4.6-fold purification was obtained in the bottom phase of ATPS including the mixture of 15% (w/w) PEG4000, 10% (w/w) Na2 SO4 and 3% (w/w) NaCl at pH 5.0. The purified enzyme was characterized regarding its activity and stability. The highest enzyme activity was observed at pH 7.0 and 37 °C on hydrogen peroxide...
February 15, 2019: Preparative Biochemistry & Biotechnology
Emer P O Connel, Vincent Healy, Fidelma Fitzpatrick, Caitriona A Higgins, John P Burke, Deborah A McNamara
BACKGROUND: Unplanned readmission after surgery negatively impacts surgical recovery. Few studies have sought to define predictors of readmission in a rectal cancer cohort alone. Readmission following rectal cancer surgery may be reduced by the identification and modification of factors associated with readmission. OBJECTIVES: This study seeks to characterize the predictors of 30-day readmission following proctectomy for rectal cancer. DESIGN: This study is a retrospective analysis of prospectively gathered cohort data...
February 8, 2019: Diseases of the Colon and Rectum
Christina M Payne, Liam P Burke, Brenton Cavanagh, Daniel O'Toole, Sally-Ann Cryan, Helena M Kelly
PURPOSE: To investigate two potential strategies aimed at targeting the inflammatory pathogenesis of COPD: a small molecule, all trans retinoic acid (atRA) and human mesenchymal stem cells (hMSCs). METHODS: atRA was formulated into solid lipid nanoparticles (SLNs) via the emulsification-ultrasonication method, and these SLNs were characterised physicochemically. Assessment of the immunomodulatory effects of atRA-SLNs on A549 cells in vitro was determined using ELISA...
February 13, 2019: Pharmaceutical Research
Aliakbar Fazaeli, Abolfazl Golestani, Mostafa Lakzaei, Samaneh Sadat Rasi Varaei, Mahdi Aminian
Cholesterol oxidase is a bifunctional bacterial flavoenzyme which catalyzes oxidation and isomerization of cholesterol. This valuable enzyme has attracted a great deal of attention because of its wide application in the clinical laboratory, synthesis of steroid derived drugs, food industries, and its potentially insecticidal activity. Therefore, development of an efficient protocol for overproduction of cholesterol oxidase could be valuable and beneficial in this regard. The present study examined the role of various parameters (host strain, culture media, induction time, isopropyl ß-D-1-thiogalactopyranoside concentration, as well as post-induction incubation time and temperature) on over-expression of cholesterol oxidase from Chromobacterium sp...
2019: PloS One
Charles S Burks, Donald R Thomson
Improved cost efficiency for aerosol mating disruption for the navel orangeworm, Amyelois transitella Walker, was examined in experiments performed between 2015 and 2017. A programmable dispenser was used to explore the effects of frequency of treatment, time of night when pheromone was emitted, and the concentration of pheromone required. A negative curvilinear trend of males captured as a function of emission frequency was evident in the range of 2-12 emissions per hour. A subsequent experiment found greater trap suppression when the same amount of active ingredient was emitted seven times per hour compared with the same amount of material emitted at twice the concentration but half the frequency...
February 11, 2019: Journal of Economic Entomology
Louise M Burke, Asker E Jeukendrup, Andrew M Jones, Martin Mooses
Distance events in Athletics include cross country, the 10,000 m track race, half marathon and marathon road races, and 20 and 50 km race walking events, over different terrain and environmental conditions. Race times for elite performers span ~26 min to >4 h, with key factors for success being a high aerobic power, the ability to exercise at a large fraction of this power, and high economy of movement. Nutrition-related contributors include body mass and anthropometry, capacity to use fuels, particularly carbohydrate (CHO) to produce ATP economically over the duration of the event, and maintenance of reasonable hydration status in the face of sweat losses induced by exercise intensity and the environment...
February 12, 2019: International Journal of Sport Nutrition and Exercise Metabolism
Mona Eini Roumiani, Niloufar Dorosti
The dandelion-shaped nanostructure of an organotin complex with formula Sn(CH3 )2 Cl2 }NC5 H4 C(O)NHP(O)[NHC6 H11 ]2 }2 (C1 ) was synthesized by means of a sonochemical method. Nano-structures were characterized by elemental analysis, NMR, SEM-EDS, XRD, UV-Vis, and FT-IR spectroscopy. The thermal stability of the complex C1 has been studied by thermal gravimetric analysis (TGA), and compared to the bulk form (C2 ). Both the morphology and the size of the ultrasound-assisted synthesized organotin complex have been investigated using scanning electron microscopy (SEM) by changing such parameters as the concentration of initial reactants and the sonication frequency...
January 23, 2019: Ultrasonics Sonochemistry
Sorena Keihani, Douglas M Rogers, Bryn E Putbrese, Rachel A Moses, Chong Zhang, Angela P Presson, James M Hotaling, Raminder Nirula, Xian Luo-Owen, Kaushik Mukherjee, Bradley J Morris, Sarah Majercik, Joshua Piotrowski, Christopher M Dodgion, Ian Schwartz, Sean P Elliott, Erik S DeSoucy, Scott Zakaluzny, Brenton G Sherwood, Bradley A Erickson, Nima Baradaran, Benjamin N Breyer, Brian P Smith, Barbara U Okafor, Reza Askari, Brandi Miller, Richard A Santucci, Matthew M Carrick, Jurek F Kocik, Timothy Hewitt, Frank N Burks, Marta E Heilbrun, Jeremy B Myers
BACKGROUND: The management of high-grade renal trauma (HGRT) and the indications for intervention are not well-defined. The American Association for the Surgery of Trauma (AAST) renal grading does not incorporate some important clinical and radiologic variables associated with increased risk of interventions. We aimed to use data from a multi-institutional contemporary cohort to develop a nomogram predicting risk of interventions for bleeding after HGRT. METHODS: From 2014 to 2017, data on adult HGRT (AAST grades III-V) were collected from 14 Level-1 trauma centers...
February 7, 2019: Journal of Trauma and Acute Care Surgery
George T Grossberg, Gary Tong, Anna D Burke, Pierre N Tariot
An estimated 47 million people live with Alzheimer's disease (AD) and other forms of dementia worldwide. Although no disease-modifying treatments are currently available for AD, earlier diagnosis and proper management of the disease could have considerable impact on patient and caregiver quality of life and functioning. Drugs currently approved for AD treat the cognitive, behavioral, and functional symptoms of the disease and consist of three cholinesterase inhibitors (ChEIs) and the N-methyl-D-aspartate receptor antagonist memantine...
February 7, 2019: Journal of Alzheimer's Disease: JAD
E Cochrane, A Menzies, K Sweeney, W Burke
•Serous endometrial carcinomas rarely involve the renal system.•Stage IVB serous endometrial carcinoma with confirmed renal metastasis.•Paclitaxel/carboplatin treating serous endometrial carcinoma with renal metastasis.
May 2019: Gynecologic Oncology Reports
Janina Burk, Heidrun Holland, Anne F Lauermann, Tobias May, Philipp Siedlaczek, Verena Charwat, Cornelia Kasper
Multipotent mesenchymal stromal cells (MSC) and MSC-derived products have emerged as promising therapeutic tools. To fully exploit their potential, further mechanistic studies are still necessary and bioprocessing needs to be optimized, which requires an abundant supply of functional MSC for basic research. To address this need, here we used a novel technology to establish a human adipose-derived MSC line with functional characteristics representative of primary MSC. Primary MSC were isolated and subjected to lentiviral transduction with a library of expansion genes...
February 10, 2019: Biotechnology and Bioengineering
A A Jum'ah, S Creanor, N H F Wilson, F J T Burke, P A Brunton
No abstract text is available yet for this article.
February 8, 2019: British Dental Journal
Li Wei, John Burke, Arnau Benet, Manish Aghi, Jose Gurrola, Ivan El-Sayed
Modern endoscopic surgery relies on capturing high fidelity images for a multitude of purposes. One method of capturing images involves incorporating infrared and 4 k resolution video in the image capture system. However, the price of such whole image capture systems remains expensive and potentially cost prohibitive for some practitioners. A major need exists for less expensive alternatives. Herein, we introduce a method of using a mirrorless camera for the purposes of an endoscope capture device. This system comprises components that are all readily commercially available...
February 2019: Journal of Neurological Surgery. Part B, Skull Base
Emmanuel O Garuba, Abiodun, A Onilude
In this study, attempts were made to immobilize purified exo-inulinase from mutant thermophic Aspergillus tamarii -U4 onto Kaolinite clay by covalent bonding cross-linked with glutaraldehyde with an immobilization yield of 66% achieved. The free and immobilized inulinases were then characterized and characterization of the enzymes revealed that temperature and pH optima for the activity of the free and immobilized enzymes were both 65 °C and pH 4.5 respectively. The free inulinase completely lost its activity after incubation at 65 °C for 6 h while the immobilized inulinase retained 16...
December 2018: Journal, Genetic Engineering & Biotechnology
Kyle M Burk, Kai Kuck, Joseph A Orr
This paper describes a method for estimating the oxygen enhanced end-tidal fraction of oxygen (Fet Oe ), the end-tidal fraction of oxygen (Fet O2 ) that is raised by administering supplemental oxygen. The paper has two purposes: the first is to evaluate the method's accuracy on the bench and in volunteers; the second purpose is to demonstrate how to apply the method to compare two techniques of oxygen administration. The method estimates Fet Oe by analyzing expired oxygen as oxygen washes out of the lung. The method for estimating Fet Oe was first validated using a bench simulation in which tracheal oxygen was measured directly...
February 6, 2019: Journal of Clinical Monitoring and Computing
N Borowiec, J La Salle, L Brancaccio, M Thaon, S Warot, M Branco, N Ris, J-C Malausa, R Burks
We report here for the first time the presence of Ophelimus mediterraneus sp. n. in Mediterranean Europe. This species appears to be closely related to Ophelimus maskelli, a well-known invasive pest of Eucalyptus. Based on molecular (cytochrome oxidase I, 28S), morphological (multivariate ratio analysis) and bio-ecological investigations, our study gives unambiguous relevant criteria that allow the discrimination between these two species. A full description of O. mediterraneus sp. n. is also provided. The geographic distribution of O...
February 6, 2019: Bulletin of Entomological Research
Hassan A Alshamrani, Hana Alloub, Derek Burke, Amaka C Offiah
BACKGROUND: Several studies have revealed a substantial increase in the incidence of fractures in children in the past few decades. AIM: To assess the strength of the association between suggested risk factors and fracture prevalence in children. METHOD: A cross sectional observational study. Children aged 6-15 years and their guardians presenting to the Emergency Department of a single tertiary paediatric hospital were recruited. Self-reported data on vitamin D intake, calcium intake and physical activity were collected...
February 6, 2019: Nutrition and Health
Taryn Lores, Charlotte Goess, Antonina Mikocka-Walus, Kathryn L Collins, Anne L J Burke, Anna Chur-Hansen, Paul Delfabbro, Jane M Andrews
Background and Aims: Inflammatory Bowel Disease is associated with psychosocial issues which reduce quality of life and impair medical management. However, these issues are rarely addressed in routine care. A model of integrated psychological screening and intervention was trialled to measure prevalence, patient participation, and potential benefits to mental health and/or quality of life. Methods: During a 12-month period, 490 adult patients at an established hospital-based service were approached to complete screening instruments for anxiety, depression, general distress, quality of life and medication adherence...
February 5, 2019: Journal of Crohn's & Colitis
Christian P Müller, Congying Chu, Liya Qin, Chunyu Liu, Bing Xu, He Gao, Barbara Ruggeri, Saskia Hieber, Julia Schneider, Tianye Jia, Nicole Tay, Shizuo Akira, Takashi Satoh, Tobias Banaschewski, Arun L W Bokde, Uli Bromberg, Christian Büchel, Erin Burke Quinlan, Herta Flor, Vincent Frouin, Hugh Garavan, Penny Gowland, Andreas Heinz, Bernd Ittermann, Jean-Luc Martinot, Marie-Laure Paillère Martinot, Eric Artiges, Herve Lemaitre, Frauke Nees, Dimitri Papadopoulos Orfanos, Tomáš Paus, Luise Poustka, Sabina Millenet, Juliane H Fröhner, Michael N Smolka, Henrik Walter, Robert Whelan, Georgy Bakalkin, Yun Liu, Sylvane Desrivières, Paul Elliott, Volker Eulenburg, Daniel Levy, Fulton Crews, Gunter Schumann
Alcohol abuse is a major public health problem worldwide. Understanding the molecular mechanisms that control regular drinking may help to reduce hazards of alcohol consumption. While immunological mechanisms have been related to alcohol drinking, most studies reported changes in immune function that are secondary to alcohol use. In this report, we analyse how the gene "TRAF family member-associated NF-κB activator" (TANK) affects alcohol drinking behavior. Based on our recent discovery in a large GWAS dataset that suggested an association of TANK, SNP rs197273, with alcohol drinking, we report that SNP rs197273 in TANK is associated both with gene expression (P = 1...
February 4, 2019: Cerebral Cortex
Manash Saha, Clement Menuet, Qi-Jian Sun, Peter G R Burke, Cara M Hildreth, Andrew M Allen, Jacqueline K Phillips
Respiratory modulation of sympathetic nerve activity (respSNA) was studied in a hypertensive rodent model of chronic kidney disease (CKD) using Lewis Polycystic Kidney (LPK) rats and Lewis controls. In adult animals under in vivo anaesthetised conditions (n = 8-10/strain), respiratory modulation of splanchnic and renal nerve activity was compared under control conditions, and during peripheral (hypoxia), and central, chemoreceptor (hypercapnia) challenge. RespSNA was increased in the LPK vs. Lewis (area under curve (AUC) splanchnic and renal: 8...
February 2, 2019: Respiratory Physiology & Neurobiology
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