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Angiogenic factors

Saereh Khanamiri, June-Wha Rhee, David T Paik, Ian Y Chen, Chun Liu, Nazish Sayed
Peripartum cardiomyopathy (PPCM) is a rare form of congestive heart failure characterized by left ventricular dysfunction that develops towards the end of pregnancy or during the early postpartum phase. Even though the majority of PPCM patients show partial or complete recovery of their heart functions, the mortality rate of PPCM remains high. Previous research has suggested that vascular dysfunction triggered by late-gestational hormones and potent anti-angiogenic factors play key roles in the pathogenesis of PPCM; however, the exact mechanisms remain elusive due to limited patient tissues for characterization...
February 14, 2019: Journal of Vascular Research
Ramla Sana Khalid, Irfan Khan, Midhat Batool Zaidi, Nadia Naeem, Kanwal Haneef, Rida-E-Maria Qazi, Rakhshinda Habib, Tuba Shakil Malick, Anwar Ali, Asmat Salim
This study was aimed to enhance the healing potential of rat bone marrow mesenchymal stem cells against chronic diabetic wounds through interleukin-7 (IL-7) transfection. IL-7 plays an important role in wound healing and acts as a survival factor in some cell types. This study involves isolation, propagation and characterization of MSCs and their modification with IL-7 gene via retroviral transfection. Transfected MSCs were assessed for their effect on angiogenic genes by qPCR. Wound healing potential of transfected MSCs was analyzed by scratch assay in vitro and by transplanting these cells in rat diabetic wound models in vivo...
February 13, 2019: Wound Repair and Regeneration
Mariya M Mikhailova, Andrey A Panteleyev, Mikhail A Paltsev, Andrey A Panteleyev
It is a well-known fact, that there is a close interconnection between vascular and neural structures in both embryonic development and postnatal life. Different models have been employed to dissect the mechanisms of these interactions, ranging from in vitro systems (e.g., co-culture of neural and endothelial cells) to in vivo imaging of central neural system recovery in laboratory animals after artificially induced trauma. Nevertheless, most of these models have serious limitations. Here, we describe an ex vivo model, representing an organotypic co-culture of aortic fragments (AF) with longitudinal slices of mouse neonatal spinal cord (SC) or dorsal root ganglia (DRG)...
February 13, 2019: Cell Biology International
Laura Benschop, Sarah Schalekamp-Timmermans, Zoe A Broere-Brown, Jeanine E Roeters van Lennep, Vincent W V Jaddoe, Jolien W Roos-Hesselink, M Kamran Ikram, Eric A P Steegers, James M Roberts, Robin E Gandley
BACKGROUND: Angiogenic placental growth factor (PlGF) concentrations rise during pregnancy, peaking at the end of mid-pregnancy. Low PlGF concentrations during pregnancy are associated with pregnancy complications with recognized later life cardiovascular risk. We hypothesized that low PlGF concentrations, especially in mid-pregnancy identify not only a subset of women at risk for pregnancy complications, but also women with greater cardiovascular risk factor burden after pregnancy regardless of pregnancy outcome...
February 14, 2019: Circulation
Ayda Pouyafar, Aysa Rezabakhsh, Reza Rahbarghazi, Milad Zadi Heydarabad, Elhameh Shokrollahi, Emel Sokullu, Majid Khaksar, Alireza Nourazarian, Çığır Biray Avci
In the current experiment, the combined regime of resveratrol and a Wnt-3a inhibitor, sulindac, were examined on the angiogenic potential of cancer stem cells from human colon adenocarcinoma cell line HT-29 during 7 days. Cancer stem cells were enriched via a magnetic-activated cell sorter technique and cultured in endothelial induction medium containing sulindac and resveratrol. Expression of endothelial markers such as the von Willebrand factor (vWF) and vascular endothelial cadherin (VE-cadherin) and genes participating in mesenchymal-to-epithelial transition was studied by real-time PCR assay...
February 13, 2019: Cell and Tissue Research
Ying Xiong, Li Liu, Yu Xia, Yangyang Qi, Yifan Chen, Lingli Chen, Peipei Zhang, Yunyi Kong, Yang Qu, Zewei Wang, Zhiyuan Lin, Xiang Chen, Zhuoyi Xiang, Jiajun Wang, Qi Bai, Weijuan Zhang, Yuanfeng Yang, Jianming Guo, Jiejie Xu
PURPOSE: Hypoxia-inducible factor 2α (HIF-2α) overexpression leads to activation of angiogenic pathways. However, little is known about the association between HIF-2α expression and anti-tumor immunity in clear cell renal cell carcinoma (ccRCC). We aimed to explore how HIF-2α influenced the microenvironment and the underlying mechanisms. EXPERIMENTAL DESIGN: We immunohistochemically evaluated immune cells infiltrations and prognostic value of HIF-2α expression in a retrospective Zhongshan Hospital cohort of 280 ccRCC patients...
February 13, 2019: Cancer Immunology, Immunotherapy: CII
Tyler D Bammert, Collin A Beckstrom, Grace Lincenberg, Jamie G Hijmans, Jared J Greiner, Natalia G Rocha
Bone marrow-derived early outgrowth cells play an important role in endothelial repair. In vitro isolation techniques have identified two distinct morphological early outgrowth cell populations, but it is still unknown whether they present some functional phenotypic differences. Accordingly, the aim of the present study was to determine whether there are phenotypic differences in cellular function between two putative early outgrowth cells in culture. Peripheral blood samples were collected from 18 healthy adults...
February 12, 2019: Cytotechnology
Aishwarya Satish, Purna Sai Korrapati
Angiogenesis is a vital component of the orchestrated wound healing cascade and tissue regeneration process, which has a therapeutic prominence in treatment of ischemic vascular diseases and certain cardiac conditions. Based on its eminence, several strategies using growth factors have been studied to initiate angiogenesis. However, growth factors are expensive and have short half-life. In this work, sustained release of triiodothyronine, which plays a crucial role in stimulating growth factors and other signaling pathways that are instrumental in initiating angiogenesis, has been attempted through electrospun polycaprolactone nanofibers...
February 12, 2019: AAPS PharmSciTech
Katarzyna Magierowska, Dominik Bakalarz, Dagmara Wójcik, Anna Chmura, Magdalena Hubalewska-Mazgaj, Sabina Licholai, Edyta Korbut, Slawomir Kwiecien, Zbigniew Sliwowski, Grzegorz Ginter, Tomasz Brzozowski, Marcin Magierowski
BACKGROUND AND PURPOSE: Besides hydrogen sulfide (H2 S) and nitric oxide (NO), carbon monoxide (CO) contributes to the maintenance of gastric mucosal integrity. We investigated increased CO bioavailability effects on time-dependent dynamics of gastric ulcer healing mediated by particular growth factors, anti-inflammatory and molecular pathways. EXPERIMENTAL APPROACH: Wistar rats with gastric ulcers induced by serosal acetic acid application (day 0) were treated i...
February 9, 2019: Biochemical Pharmacology
Karla B Neves, Francisco J Rios, Robert Jones, T R Jeffry Evans, Augusto C Montezano, Rhian M Touyz
Vascular endothelial growth factor pathway inhibitors (VEGFi), used as anti-angiogenic drugs to treat cancer are associated with cardiovascular toxicities through unknown molecular mechanisms. Endothelial cell-derived microparticles (ECMPs) are biomarkers of endothelial injury and are also functionally active since they influence downstream target cell signaling and function. We questioned whether microparticle (MP) status is altered in cancer patients treated with VEGFi and whether they influence endothelial cell function associated with vascular dysfunction...
February 11, 2019: Cardiovascular Research
Hans Prenen, Massimiliano Mazzone
Macrophages play an important role in tissue development and homeostasis. They serve as a nexus between adaptive and innate immunity, and employ considerable plasticity. In cancer, they play a pivotal role in chronic inflammation and tumor growth either by directly stimulating the proliferation of cancer cells or by producing angiogenic and lymphangiogenic factors. Although numerous immune cells play an important role in the tumor microenvironment, tumor-associated macrophages (TAMs) are by far the most extensively studied...
February 12, 2019: Cellular and Molecular Life Sciences: CMLS
Amita Ajit, Santhoshkumar Thankayyan Retnabai, Lissy K Krishnan
Biotechnological interventions to engineer favorable biochemical environments that facilitate wound repair through quick attainment of vasculature are of immense demand. Potential of angiogenic factors (AF), notably vascular endothelial growth factor A (VEGF-A) and hypoxia-inducible factor-1α (Hif-1α) and the paracrine role of human adipose derived mesenchymal stromal cells (hADMSCs) in establishing vasculature has been reported. Independent application of AF or hADMSCs has led to clinical failure, pertaining to poor means of delivery, deprived bioavailability and insufficiency to drive adequate angiogenesis, respectively...
February 12, 2019: Tissue Engineering. Part C, Methods
Sam R Emerson, Christina M Sciarrillo, Stephanie P Kurti, Emily M Emerson, Sara K Rosenkranz
Background: Inflammation and angiogenesis are key facets of cardiovascular disease pathophysiology. Age and physical activity level can influence fasting systemic inflammation, but the impact of these factors on postprandial inflammation is unknown. In addition, markers of angiogenesis have never been tested in the context of a single high-fat meal (HFM). Objective: The purpose of this study was to investigate the effects of an HFM on markers of inflammation and angiogenesis in individuals of different ages and physical activity levels...
January 2019: Current developments in nutrition
Chengqian Dai, Shu-Jui Kuo, Jin Zhao, Lulu Jin, Le Kang, Lihong Wang, Guohong Xu, Chih-Hsin Tang, Chen-Ming Su
The Angiopoietin-2 (Ang2) gene encodes angiogenic factor, and the polymorphisms of Ang2 gene predict risk of various human diseases. We want to investigate whether the single nucleotide polymorphisms (SNPs) of the Ang2 gene can predict the risk of rheumatoid arthritis (RA). Between 2016 and 2018, we recruited 335 RA patients and 700 control participants. Comparative genotyping for SNPs rs2442598, rs734701, rs1823375 and rs12674822 was performed. We found that when compared with the subjects with the A/A genotype of SNP rs2442598, the subjects with the T/T genotype were 1...
2019: International Journal of Medical Sciences
Mizuho Ishikawa, Mitsuhiko Osaki, Makoto Yamagishi, Kunishige Onuma, Hisao Ito, Futoshi Okada, Hideya Endo
Extensive studies on metastasis-associated proteins, S100A4 and MTA1, have been carried out for over two decades, but correlation of both proteins remains obscure. Here we show evidence for the correlation in angiogenesis. First, silencing of each protein by siRNA-mediated knockdown in mouse endothelial MSS31 cells resulted in the inhibition of tube formation. Unexpectedly, the knockdown of MTA1 affected not only its own expression but also the expression of S100A4, whereas silencing of S100A4 did not affect the MTA1 expression...
February 11, 2019: Oncogene
Dorottya Kardos, Melinda Simon, Gabriella Vácz, Adél Hinsenkamp, Tünde Holczer, Domonkos Cseh, Adrienn Sárközi, Kálmán Szenthe, Ferenc Bánáti, Susan Szathmary, Stefan Nehrer, Olga Kuten, Mariana Masteling, Zsombor Lacza, István Hornyák
Autologous blood derived products, such as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are widely applied in regenerative therapies, in contrast to the drawbacks in their application, mainly deriving from the preparation methods used. Eliminating the disadvantages of both PRP and PRF, hyperacute serum (HAS) opens a new path in autologous serum therapy showing similar or even improved regenerative potential at the same time. Despite the frequent experimental and clinical use of PRP and HAS, their protein composition has not been examined thoroughly yet...
February 8, 2019: International Journal of Molecular Sciences
Di Wang, Jian Zheng, Xiaobai Liu, Yixue Xue, Libo Liu, Jun Ma, Qianru He, Zhen Li, Heng Cai, Yunhui Liu
The anti-angiogenic treatment of malignant glioma cells is an effective method to treat high-grade gliomas. However, due to the presence of vasculogenic mimicry (VM), the anti-angiogenic treatment of gliomas is not significantly effective in improving overall patient median survival. Therefore, this study investigated the mechanism of mimic formation of angiogenesis in gliomas. The results of this experiment indicate that the expression of upstream transcription factor 1 (USF1) is upregulated in glioma tissues and cells...
January 15, 2019: Molecular Therapy. Nucleic Acids
Krzysztof Gomułka, Jerzy Liebhart, Urszula Gładysz, Wojciech Mędrala
BACKGROUND: Vascular endothelial growth factor (VEGF) is an angiogenic, heparin-binding glycoprotein playing an important role in the pathogenesis of many diseases and disorders, including asthma. It has been reported that increased VEGF serum concentration is a biomarker of neovascularization, which could suggest that higher VEGF expression may be relevant to asthmatics with airway remodeling and irreversible bronchoconstriction. OBJECTIVES: The aim of this study was to assess the possible association between VEGF serum concentration and irreversible bronchoconstriction in adult patients with a diagnosis of asthma...
February 8, 2019: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
Shengli Zhao, Jie Yin, Qinghe Yu, Guowei Zhang, Shaoxiong Min
Objective: To observe the effect of vascular endothelial growth factor/polylactide-polyethyleneglycol-polylactic acid copolymer/basic fibroblast growth factor (VEGF/PELA/bFGF) mixed microcapsules in promoting the angiogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in vitro . Methods: The BMSCs were isolated by the method of whole bone marrow adherent, and sub-cultured. The passage 3 BMSCs were identified by Wright-Giemsa staining and flow cytometry, and used for subsequent experiments...
February 1, 2019: Chinese Journal of Reparative and Reconstructive Surgery
Srinivas V Koduru, Ashley N Leberfinger, Denis Pasic, Anoosha Forghani, Shane Lince, Daniel J Hayes, Ibrahim T Ozbolat, Dino J Ravnic
Vascularization is a major hurdle in complex tissue and organ engineering. Tissues greater than 200 μm in diameter cannot rely on simple diffusion to obtain nutrients and remove waste. Therefore, an integrated vascular network is required for clinical translation of engineered tissues. Microvessels have been described as <150 μm in diameter, but clinically they are defined as <1 mm. With new advances in super microsurgery, vessels less than 1 mm can be anastomosed to the recipient circulation. However, this technical advancement still relies on the creation of a stable engineered microcirculation that is amenable to surgical manipulation and is readily perfusable...
February 10, 2019: Stem Cell Reviews
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