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Kazumichi M Nishida, Kazuhiro Sakakibara, Yuka W Iwasaki, Hiromi Yamada, Ryo Murakami, Yukiko Murota, Takeshi Kawamura, Tatsuhiko Kodama, Haruhiko Siomi, Mikiko C Siomi
PIWI-interacting RNAs (piRNAs) are small regulatory RNAs that bind to PIWI proteins to control transposons and maintain genome integrity in animal germ lines. piRNA 3' end formation in the silkworm Bombyx mori has been shown to be mediated by the 3'-to-5' exonuclease Trimmer (Trim; known as PNLDC1 in mammals), and piRNA intermediates are bound with PIWI anchored onto mitochondrial Tudor domain protein Papi. However, it remains unclear whether the Zucchini (Zuc) endonuclease and Nibbler (Nbr) 3'-to-5' exonuclease, both of which have pivotal roles in piRNA biogenesis in Drosophila, are required for piRNA processing in other species...
March 8, 2018: Nature
Alfred W Bronkhorst, René F Ketting
No abstract text is available yet for this article.
March 2018: EMBO Reports
Toru Nishimura, Ippei Nagamori, Tsunetoshi Nakatani, Natsuko Izumi, Yukihide Tomari, Satomi Kuramochi-Miyagawa, Toru Nakano
PIWI-interacting RNAs (piRNAs) are germ cell-specific small RNAs essential for retrotransposon gene silencing and male germ cell development. In piRNA biogenesis, the endonuclease MitoPLD/Zucchini cleaves long, single-stranded RNAs to generate 5' termini of precursor piRNAs (pre-piRNAs) that are consecutively loaded into PIWI-family proteins. Subsequently, these pre-piRNAs are trimmed at their 3'-end by an exonuclease called Trimmer. Recently, poly(A)-specific ribonuclease-like domain-containing 1 (PNLDC1) was identified as the pre-piRNA Trimmer in silkworms...
March 2018: EMBO Reports
Deqiang Ding, Jiali Liu, Kunzhe Dong, Uros Midic, Rex A Hess, Huirong Xie, Elena Y Demireva, Chen Chen
Piwi-interacting RNAs are small regulatory RNAs with key roles in transposon silencing and regulation of gametogenesis. The production of mature piwi-interacting RNAs requires a critical step of trimming piwi-interacting RNA intermediates to achieve optimally sized piwi-interacting RNAs. The poly(A)-specific ribonuclease family deadenylase PNLDC1 is implicated in piwi-interacting RNA trimming in silkworms. The physiological function of PNLDC1 in mammals remains unknown. Using Pnldc1-deficient mice, here we show that PNLDC1 is required for piwi-interacting RNA biogenesis, transposon silencing, and spermatogenesis...
October 10, 2017: Nature Communications
Yue Zhang, Rui Guo, Yiqiang Cui, Zhiping Zhu, Yingwen Zhang, Hao Wu, Bo Zheng, Qiuling Yue, Shun Bai, Wentao Zeng, Xuejiang Guo, Zuomin Zhou, Bin Shen, Ke Zheng, Mingxi Liu, Lan Ye, Jiahao Sha
No abstract text is available yet for this article.
November 2017: Cell Research
Ilias Skeparnias, Dimitrios Αnastasakis, Athanasios-Nasir Shaukat, Katerina Grafanaki, Constantinos Stathopoulos
Deadenylases belong to an expanding family of exoribonucleases involved mainly in mRNA stability and turnover, with the exception of PARN which has additional roles in the biogenesis of several important non-coding RNAs, including miRNAs and piRNAs. Recently, PARN in C. elegans and its homolog PNLDC1 in B. mori were reported as the elusive trimmers mediating piRNA biogenesis. In addition, characterization of mammalian PNLDC1 in comparison to PARN, showed that is specifically expressed in embryonic stem and germ cells, as well as during early embryo development...
October 3, 2017: RNA Biology
Dimitrios Anastasakis, Ilias Skeparnias, Athanasios-Nasir Shaukat, Katerina Grafanaki, Alexandra Kanellou, Stavros Taraviras, Dionysios J Papachristou, Athanasios Papakyriakou, Constantinos Stathopoulos
PNLDC1 is a homologue of poly(A) specific ribonuclease (PARN), a known deadenylase with additional role in processing of non-coding RNAs. Both enzymes were reported recently to participate in piRNA biogenesis in silkworm and C. elegans, respectively. To get insights on the role of mammalian PNLDC1, we characterized the human and mouse enzymes. PNLDC1 shows limited conservation compared to PARN and represents an evolutionary related but distinct group of enzymes. It is expressed specifically in mouse embryonic stem cells, human and mouse testes and during early mouse embryo development, while it fades during differentiation...
October 14, 2016: Nucleic Acids Research
Christian G Bouwkamp, Anneke J A Kievit, Simone Olgiati, Guido J Breedveld, Michiel Coesmans, Vincenzo Bonifati, Steven A Kushner
Affective psychoses are a group of severe psychiatric disorders, including schizoaffective disorder and bipolar I disorder, together affecting ∼1% of the population. Despite their high heritability, the molecular genetics and neurobiology of affective psychosis remain largely elusive. Here, we describe the identification of a structural genetic variant segregating with affective psychosis in a family with multiple members suffering from bipolar I disorder or schizoaffective disorder, bipolar type. A balanced translocation involving chromosomes 6 and 15 was detected by karyotyping and fluorescence in-situ hybridization (FISH)...
April 2017: American Journal of Medical Genetics. Part B, Neuropsychiatric Genetics
Natsuko Izumi, Keisuke Shoji, Yuriko Sakaguchi, Shozo Honda, Yohei Kirino, Tsutomu Suzuki, Susumu Katsuma, Yukihide Tomari
PIWI-interacting RNAs (piRNAs) play a crucial role in transposon silencing in animal germ cells. In piRNA biogenesis, single-stranded piRNA intermediates are loaded into PIWI-clade proteins and cleaved by Zucchini/MitoPLD, yielding precursor piRNAs (pre-piRNAs). Pre-piRNAs that are longer than the mature piRNA length are then trimmed at their 3' ends. Although recent studies implicated the Tudor domain protein Papi/Tdrkh in pre-piRNA trimming, the identity of Trimmer and its relationship with Papi/Tdrkh remain unknown...
February 25, 2016: Cell
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