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Adipose Stromal Cells OR Adipose Stem Cells AND Burn OR Burns

Anesh Prasai, Amina El Ayadi, Randy C Mifflin, Michael D Wetzel, Clark R Andersen, Heinz Redl, David N Herndon, Celeste C Finnerty
Severe burns induce a prolonged inflammatory response in subcutaneous adipose tissue that modulates signaling in adipose-derived stem cells (ASCs), which hold potential for healing burn wounds or generating skin substitutes. Using a 60% rat scald burn model, we conducted a series of experiments to determine which cells isolated from the adipose tissue produced inflammatory mediators and how these changes affect ASC fate and function. The stromal vascular fraction (SVF), adipocytes, and ASCs were isolated from adipose tissue at varying times up to 4 weeks postburn and from non-injured controls...
December 2017: Stem Cell Reviews
Yumiko Oishi, Ichiro Manabe
Adipose tissues are the major organ that controls systemic energy metabolism and maintain homeostasis by storing lipids, dissipating them as heat, and producing various adipokines. There are two major classes of adipocytes: white and brown adipocytes. White adipocytes store and release lipids, while brown adipocytes burn substrates to produce heat. In addition to classical brown adipose tissues consisting of brown adipocytes, cold exposure and β3 stimulation induce development of brown cell-like "beige" adipocytes in white adipose tissues...
2017: Clinical Calcium
Agnes S Klar, Jakub Zimoch, Thomas Biedermann
Perception of the adipose tissue has changed dramatically over the last few decades. Identification of adipose-derived stem cells (ASCs) ultimately transformed paradigm of this tissue from a passive energy depot into a promising stem cell source with properties of self-renewal and multipotential differentiation. As compared to bone marrow-derived stem cells (BMSCs), ASCs are more easily accessible and their isolation yields higher amount of stem cells. Therefore, the ASCs are of high interest for stem cell-based therapies and skin tissue engineering...
2017: BioMed Research International
Kavitha Ranganathan, Shailesh Agarwal, David Cholok, Shawn Loder, Jonathan Li, Hsiao Hsin Sung Hsieh, Stewart C Wang, Steven R Buchman, Benjamin Levi
BACKGROUND: Heterotopic ossification (HO) is the pathologic process of extraskeletal bone formation. Although the exact etiology remains unknown, inflammation appears to catalyze disease progression. The goal of this study is to determine the impact of the adaptive immune system on HO. METHODS: HO was induced in 8-wk-old control C57BL/6 and immunocompromised Rag1tm1Mom (Rag1 KO) male mice deficient in B- and T-lymphocytes via combined Achilles tenotomy and burn injury...
November 2016: Journal of Surgical Research
Sophie Domergue, Claire Bony, Marie Maumus, Karine Toupet, Eric Frouin, Valérie Rigau, Marie-Catherine Vozenin, Guy Magalon, Christian Jorgensen, Danièle Noël
Hypertrophic scars (HTS) are characterized by excessive amount of collagen deposition and principally occur following burn injuries or surgeries. In absence of effective treatments, the use of mesenchymal stem/stromal cells, which have been shown to attenuate fibrosis in various applications, seems of interest. The objectives of the present study were therefore to evaluate the effect of human adipose tissue-derived mesenchymal stem cells (hASC) on a pre-existing HTS in a humanized skin graft model in Nude mice and to compare the efficacy of hASCs versus stromal vascular fraction (SVF)...
2016: PloS One
Mariana Teixeira Cerqueira, Rogério Pedro Pirraco, Alexandra Pinto Marques
Significance: Cutaneous wound healing is a serious problem worldwide that affects patients with various wound types, resulting from burns, traumatic injuries, and diabetes. Despite the wide range of clinically available skin substitutes and the different therapeutic alternatives, delayed healing and scarring are often observed. Recent Advances: Stem cells have arisen as powerful tools to improve skin wound healing, due to features such as effective secretome, self-renewal, low immunogenicity, and differentiation capacity...
April 1, 2016: Advances in Wound Care
M Gholipourmalekabadi, M Sameni, Dina Radenkovic, M Mozafari, M Mossahebi-Mohammadi, A Seifalian
OBJECTIVES: Human amniotic membrane (HAM) has been widely used in soft tissue engineering both in its fresh form and decellularized; its efficiency to aid treatment of burn injuries is well known. On the other hand, it has been reported clinically by several studies that human adipose-derived stem cells (hADSC) are a promising cell source for cell therapy for burns. Recently, we have reported a new technique for decellularization of HAM. In this study, potential of prepared decellularized HAM (dHAM) as a viable support for proliferation and delivery of hADSC was investigated...
February 2016: Cell Proliferation
Andrew Nguyen, James Guo, Derek A Banyard, Darya Fadavi, Jason D Toranto, Garrett A Wirth, Keyianoosh Z Paydar, Gregory R D Evans, Alan D Widgerow
Stromal Vascular Fraction (SVF) is a heterogeneous collection of cells contained within adipose tissue that is traditionally isolated using enzymes such as collagenase. With the removal of adipose cells, connective tissue and blood from lipoaspirate, comes the SVF, a mix including mesenchymal stem cells, endothelial precursor cells, T regulatory cells, macrophages, smooth muscle cells, pericytes and preadipocytes. In part 1 of our 2-part series, we review the literature with regards to the intensifying interest that has shifted toward this mixture of cells, particularly due to its component synergy and translational potential...
February 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
James Guo, Andrew Nguyen, Derek A Banyard, Darya Fadavi, Jason D Toranto, Garrett A Wirth, Keyianoosh Z Paydar, Gregory R D Evans, Alan D Widgerow
Adipose tissue is a rich source of cells with emerging promise for tissue engineering and regenerative medicine. The stromal vascular fraction (SVF), in particular, is an eclectic composite of cells with progenitor activity that includes preadipocytes, mesenchymal stem cells, pericytes, endothelial cells, and macrophages. SVF has enormous potential for therapeutic application and is being investigated for multiple clinical indications including lipotransfer, diabetes-related complications, nerve regeneration, burn wounds and numerous others...
February 2016: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Roberto Sánchez-Sánchez, Ana Brena-Molina, Valentín Martínez-López, Yaaziel Melgarejo-Ramírez, Lenin Tamay de Dios, Ricardo Gómez-García, Ma de Lourdes Reyes-Frías, Lourdes Rodríguez-Rodríguez, David Garciadiego-Cázares, Haydée Lugo-Martínez, Clemente Ibarra, María Esther Martínez-Pardo, Cristina Velasquillo-Martínez
Human adipose-derived mesenchymal stem cells (hADMSCs) are believed to be potential key factors for starting the regenerative process after tissue injury. However, an efficient method of delivering these regenerative cells to an external wound site is still lacking. Human amnion and pig skin have long been used as skin wound dressings for the treatment of burns and other skin lesions. Herein, we present the generation of two constructs using these two biomaterials as effective scaffolds for the culture of hADMSCs...
November 2015: ASAIO Journal: a Peer-reviewed Journal of the American Society for Artificial Internal Organs
Vladimir Holan, Peter Trosan, Cestmir Cejka, Eliska Javorkova, Alena Zajicova, Barbora Hermankova, Milada Chudickova, Jitka Cejkova
UNLABELLED: Stem cell-based therapy has become an attractive and promising approach for the treatment of severe injuries or thus-far incurable diseases. However, the use of stem cells is often limited by a shortage of available tissue-specific stem cells; therefore, other sources of stem cells are being investigated and tested. In this respect, mesenchymal stromal/stem cells (MSCs) have proven to be a promising stem cell type. In the present study, we prepared MSCs from bone marrow (BM-MSCs) or adipose tissue (Ad-MSCs) as well as limbal epithelial stem cells (LSCs), and their growth, differentiation, and secretory properties were compared...
September 2015: Stem Cells Translational Medicine
Jonathan R Peterson, Oluwatobi N Eboda, R Cameron Brownley, Katherine E Cilwa, Lauren E Pratt, Sara De La Rosa, Shailesh Agarwal, Steven R Buchman, Paul S Cederna, Michael D Morris, Stewart C Wang, Benjamin Levi
Heterotopic ossification (HO) is a common and debilitating complication of burns, traumatic brain injuries, and musculoskeletal trauma and surgery. Although the exact mechanism of ectopic bone formation is unknown, mesenchymal stem cells (MSCs) capable of osteogenic differentiation are known to play an essential role. Interestingly, the prevalence of HO in the elderly population is low despite the high overall occurrence of musculoskeletal injury and orthopedic procedures. We hypothesized that a lower osteogenicity of MSCs would be associated with blunted HO formation in old compared with young mice...
January 15, 2015: Stem Cells and Development
Isabel Sánchez-Muñoz, Rosario Granados, Purificación Holguín Holgado, José Antonio García-Vela, Celia Casares, Miguel Casares
In recent years, the reconstruction of human skin by tissue engineering represents a clinical challenge and has offered a therapeutic alternative. Avascular engineered skin equivalents have been available for several years and used to treat wounds due to burns, nonhealing ulcers, and surgical excisions. They are constituted by different types of cultured cells included in a three-dimensional structure that permits cellular proliferation to create tissue substitutes. The major drawback of these artificial skin substitutes is their lack of blood supply, since the endurance and cell proliferation of the substitute depend on an adequate oxygen and nutrient supply and on toxin removal...
January 2015: Tissue Engineering. Part A
Cristiano Rodrigues, Adriano M de Assis, Dinara J Moura, Graziele Halmenschlager, Jenifer Saffi, Léder Leal Xavier, Marilda da Cruz Fernandes, Márcia Rosângela Wink
Lesions with great loss of skin and extensive burns are usually treated with heterologous skin grafts, which may lead rejection. Cell therapy with mesenchymal stem cells is arising as a new proposal to accelerate the healing process. We tested a new therapy consisting of sodium carboxymethylcellulose (CMC) as a biomaterial, in combination with adipose-derived stem cells (ADSCs), to treat skin lesions in an in vivo rat model. This biomaterial did not affect membrane viability and induced a small and transient genotoxicity, only at the highest concentration tested (40 mg/mL)...
2014: PloS One
Sibel Atalay, Atilla Coruh, Kemal Deniz
OBJECTIVE: The practice of early burn wound excision and wound closure by immediate autologous skin or skin substitutes is the preferred treatment in extensive deep partial and full-thickness burns. To date there is no proven definite medical treatment to decrease burn wound size and accelerate burn wound healing in modern clinical practice. Stromal vascular fraction is an autologous mixture that has multiple proven beneficial effects on different kinds of wounds. In our study, we investigated the effects of stromal vascular fraction on deep partial-thickness burn wound healing...
November 2014: Burns: Journal of the International Society for Burn Injuries
Pietro Gentile, Barbara De Angelis, Methap Pasin, Giulio Cervelli, Cristiano B Curcio, Micol Floris, Camilla Di Pasquali, Ilaria Bocchini, Alberto Balzani, Fabio Nicoli, Chiara Insalaco, Eleonora Tati, Lucilla Lucarini, Ludovico Palla, Michele Pascali, Pamela De Logu, Chiara Di Segni, Davide J Bottini, Valerio Cervelli
BACKGROUND: Actually, autologous fat grafts have many clinical applications in breast surgery, facial rejuvenation, buttock augmentation, and Romberg syndrome as well as a treatment of liposuction sequelae. OBJECTIVE: The aim of this article was to describe the preparation and isolation procedures for stromal vascular fraction (SVF), the preparation of platelet-rich plasma (PRP), and the clinical application in the treatment of the scar on the face. METHODS: Ten patients with burns sequelae (n = 6) and post-traumatic scars (n = 4) were treated with SVF-enhanced autologous fat grafts obtained by the Celution System...
January 2014: Journal of Craniofacial Surgery
Lenie J van den Broek, Kim L Kroeze, Taco Waaijman, Melanie Breetveld, Shakun C Sampat-Sardjoepersad, Frank B Niessen, Esther Middelkoop, Rik J Scheper, Susan Gibbs
Many cell-based regenerative medicine strategies toward tissue-engineered constructs are currently being explored. Cell-cell interactions and interactions with different biomaterials are extensively investigated, whereas very few studies address how cultured cells will interact with soluble wound-healing mediators that are present within the wound bed after transplantation. The aim of this study was to determine how adipose tissue-derived mesenchymal stem cells (ASC), dermal fibroblasts, and keratinocytes will react when they come in contact with the deep cutaneous burn wound bed...
January 2014: Tissue Engineering. Part A
Maria Serena Piccinno, Elena Veronesi, Pietro Loschi, Marco Pignatti, Alba Murgia, Giulia Grisendi, Ilaria Castelli, Daniela Bernabei, Olivia Candini, Pierfranco Conte, Paolo Paolucci, Edwin M Horwitz, Giorgio De Santis, Lorenzo Iughetti, Massimo Dominici
Autologous fat transfer (AFT) is a procedure for adipose tissue (AT) repair after trauma, burns, post-tumor resections and lipodystrophies still negatively impacted by the lack of graft persistence. The reasons behind this poor outcome are unclear and seem to involve damages in either harvested/transplanted mature adipocytes or on their mesenchymal progenitors, namely adipose stromal/stem cells (ASC), and due to post-transplant AT apoptosis and involution. A rabbit subcutaneous AT regeneration model was here developed to first evaluate graft quality at different times after implant focusing on related parameters, such as necrosis and vasculogenesis...
October 2013: Apoptosis: An International Journal on Programmed Cell Death
Emilia Guirguis, Steven Hockman, Youn Wook Chung, Faiyaz Ahmad, Oksana Gavrilova, Nalini Raghavachari, Yanqin Yang, Gang Niu, Xiaoyuan Chen, Zu Xi Yu, Shiwei Liu, Eva Degerman, Vincent Manganiello
Obesity is linked to various diseases, including insulin resistance, diabetes, and cardiovascular disorders. The idea of inducing white adipose tissue (WAT) to assume characteristics of brown adipose tissue (BAT), and thus gearing it to fat burning instead of storage, is receiving serious consideration as potential treatment for obesity and related disorders. Phosphodiesterase 3B (PDE3B) links insulin- and cAMP-signaling networks in tissues associated with energy metabolism, including WAT. We used C57BL/6 PDE3B knockout (KO) mice to elucidate mechanisms involved in the formation of BAT in epididymal WAT (EWAT) depots...
September 2013: Endocrinology
Filippo Rossignoli, Anna Caselli, Giulia Grisendi, Serena Piccinno, Jorge S Burns, Alba Murgia, Elena Veronesi, Pietro Loschi, Cristina Masini, Pierfranco Conte, Paolo Paolucci, Edwin M Horwiz, Massimo Dominici
Mesenchymal stromal/stem cells (MSCs) reveal progenitor cells-like features including proliferation and differentiation capacities. One of the most historically recognized sources of MSC has been the bone marrow, while other sources recently include adipose tissue, teeth, bone, muscle, placenta, liver, pancreas, umbilical cord, and cord blood. Frequently, progenitor isolation requires traumatic procedures that are poorly feasible and associated with patient discomfort. In the attempt to identify a more approachable MSC source, we focused on endometrial decidual tissue (EDT) found within menstrual blood...
2013: BioMed Research International
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