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Neeraj Kumar, Annie van Dam, Hjalmar Permentier, Martijn van Faassen, Ido Kema, Manfred Gahr, Ton G G Groothuis
Several studies show effects of yolk androgens in avian eggs on the phenotype of the offspring. Yolk hormone concentrations decline strongly already in the first few days of incubation. Although early embryonic uptake of yolk androgens is suggested by the presence of radioactivity in the embryo when eggs are injected with radiolabelled androgens, these studies do not verify the chemical identity of radioactive compound(s), while it is known that these androgens can be metabolized substantially. By using stable isotope labelled testosterone and androstenedione in combination with mass spectrometry, enabling verification of the exact molecular identity of labelled compounds in the embryo, we found that after five days of incubation the androgens are not taken up by the embryo...
March 12, 2019: Journal of Experimental Biology
P Maidana, A Fritzler, Y Mocarbel, M B Perez Lana, D González, M Rosales, F González de Chazal, K Sternberg, E Lamas Majek, S Mallea-Gil, E Genovesi, M Pelayo, B Fabre, V Mesch
Vitamin D deficiency has been related with metabolic alterations in polycystic ovary syndrome (PCOS). As well, hyperactivation of adrenal axis can be programmed early in life and could be related later with PCOS development. Our aim was to establish the relationship between vitamin D and adrenal parameters with metabolic alterations and inflammation markers in PCOS. In 73 patients and 33 controls, 25-hydroxyvitamin D (25-OH-D), total and bioavailable testosterone (TT and bioT), androstenedione (A4), SHBG, cortisol, insulin, and C-reactive protein (hs-CRP) were determined; HOMA and lipid accumulation product (LAP) index were calculated...
March 8, 2019: Scientific Reports
Bingjie Zhang, Shanmei Shen, Tianwei Gu, Ting Hong, Jiayi Liu, Jie Sun, Hongdong Wang, Yan Bi, Dalong Zhu
Polycystic ovary syndrome (PCOS) is a common metabolic disorder with hyperandrogenism as the core pathophysiologic feature. Increased bile acids and altered bile acid pool composition have been implicated in the pathogenesis of several metabolic diseases. Thus, this study aimed to clarify the specific alterations in circulating bile acids in patients with PCOS and investigate its associations with hyperandrogenism. We recruited 37 patients with PCOS and 35 age- and BMI-matched control subjects. Serum bile acids and androgen profiles were assessed by ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC-MS/MS)...
March 5, 2019: Journal of Steroid Biochemistry and Molecular Biology
Susan R Davis, Adina F Turcu, Penelope J Robinson, Robin J Bell
Context: 11 β -Hydroxyandrostenedione (11OHA4), 11 β -hydroxytestosterone (11OHT), and their respective peripheral derivatives, 11-ketoandrostenedione (11KA4) and 11-ketotesosterone (11KT), have been implicated in androgen-related physiopathology. Little is known of these steroids in postmenopausal women or whether exogenous testosterone therapy influences their levels. Objective: The impact of exogenous testosterone on serum levels of 11-oxygenated steroids was determined in healthy postmenopausal women...
March 1, 2019: Journal of the Endocrine Society
Rachelle Gent, Therina du Toit, Liezl M Bloem, Amanda C Swart
The 11β-hydroxysteroid dehydrogenase (11βHSD) types 1 and 2 are primarily associated with glucocorticoid inactivation and reactivation. Several adrenal C11-oxy C19 and C11-oxy C21 steroids, which have been identified in prostate cancer, 21-hydroxylase deficiency and polycystic ovary syndrome, are substrates for these isozymes. This study describes the kinetic parameters of 11βHSD1 and 11βHSD2 towards the C11-keto and C11-hydroxy derivatives of the C19 and C21 steroids. The apparent Km and Vmax values indicate the more prominent 11βHSD2 activity towards 11β-hydroxy androstenedione, 11β-hydroxytestosterone and 11β-hydroxyprogesterone in contrast to the 11βHSD1 reduction of the C11-keto steroids, as was demonstrated in the LNCaP cell model in the production of 11-ketotestosterone and 11-ketodihydrotestosterone...
February 27, 2019: Journal of Steroid Biochemistry and Molecular Biology
Fahimeh Ramezani-Tehrani, Khaled Rahmani, Ali Moradi, Seyed Ali Montazeri, Razieh Bidhendi-Yarandi, Fatemeh Darabi
Objective: To assess and compare the normal ranges of androgen hormones level, total testosterone (TT), free testosterone (FT), dehydrotestosterone (DHT), androstenedione (A4), dehydroepiandrosterone (DHEA), and dehydroepiandrosterone sulfate (DHEAS), in Iranian women based on different statistical methods. Materials and methods: This study was conducted on previous data collected in Iranian PCOS Prevalence Study, which details have been published before. A total of 1772 women of 18-45 years were recruited from urban areas of five randomly selected provinces in different geographic regions of Iran...
June 2018: Journal of Family & Reproductive Health
Andreas Krebs, Karoline Dickhuth, Rebekka Mumm, Bernhard Stier, Jürgen Doerfer, Dirk Grueninger, Michael Wurm, Corinna Brichta, Karl Otfried Schwab
Background During pubertal development in healthy boys, increased levels of different sex steroids occur which are responsible for sexual maturation and physical changes. However, relationships between various sex hormones and pubertal development stages have not been sufficiently studied. Methods The investigation included 165 normal boys (mean age 12.7±2.8 years, mean body mass index [BMI] 19.6±4.2 kg/m2). Pubic hair (PH) stages were stratified by Tanner and testicular volume (TV) by means of the Prader orchidometer and assigned to the prepubertal, pubertal and postpubertal development phase...
February 23, 2019: Journal of Pediatric Endocrinology & Metabolism: JPEM
Lauren C Houghton, Julia A Knight, Ying Wei, Russell D Romeo, Mandy Goldberg, Irene L Andrulis, Angela R Bradbury, Saundra S Buys, Mary B Daly, Esther M John, Wendy K Chung, Regina M Santella, Frank Z Stanczyk, Mary Beth Terry
Importance: Early breast development is a risk factor for breast cancer, and girls with a breast cancer family history (BCFH) experience breast development earlier than girls without a BCFH. Objectives: To assess whether prepubertal androgen concentrations are associated with timing of breast development (analysis 1) and to compare serum androgen concentrations in girls with and without a BCFH (analysis 2). Design, Setting, and Participants: Prospective cohort study of 104 girls aged 6 to 13 years at baseline using data collected between August 16, 2011, and March 24, 2016, from the Lessons in Epidemiology and Genetics of Adult Cancer From Youth (LEGACY) Girls Study, New York site...
February 1, 2019: JAMA Network Open
Sofia Galanou, Giorgos Chouliaras, Panagiotis Girginoudis, Chryssanthi Mengreli, Amalia Sertedaki, Maria Dracopoulou, Ioanna Farakla, Dimitris Platis, Alexandra Iliadi, George P Chrousos, Catherine Dacou-Voutetakis, Emanuil Zoumakis, Alexandra-Maria Magiakou, Christina Kanaka-Gantenbein, Antonis Voutetakis
CONTEXT: The adrenal gland undergoes significant remodeling during the neonatal period, an essential developmental process that still remains incompletely understood. With respect to control over the remodeling process and, specifically, the role of thyroid hormones (TH), no human studies have been published. The effect of both hypo- and hyper-thyroidism has only been evaluated in adults, focusing on the mature adrenal. Recently, Huang et al identified expression of the TH receptor β1 in the mouse adrenal X-zone and demonstrated that TH administration could alter the postnatal adrenal remodeling process...
February 20, 2019: Journal of Clinical Endocrinology and Metabolism
Bar Neeman, Rachel Bello, Liora Lazar, Moshe Phillip, Liat de Vries
CONTEXT: Central precocious puberty (CPP) may be the first presentation of non-classical congenital adrenal hyperplasia (NCCAH) in girls. Data on the prevalence and the clinical phenotype of CPP associated with NCCAH are sparse. OBJECTIVES: To study the clinical and laboratory characteristics that could differentiate idiopathic CPP from CPP associated with NCCAH and to determine the prevalence of NCCAH among girls with CPP. DESIGN: Case-control study...
February 19, 2019: Journal of Clinical Endocrinology and Metabolism
Aya T Nanba, Juilee Rege, Jianwei Ren, Richard J Auchus, William E Rainey, Adina F Turcu
Context: The ovaries and adrenals are sources of androgens in women. While dehydroepiandrosterone (DHEA), DHEA sulfate (DHEAS), and testosterone (T) all decline with age, these C19 steroids correlate poorly with parameters of androgen action in postmenopausal women. Objective: To comprehensively compare androgen profiles in pre- and post-menopausal women. Methods: We quantified 19 steroids, including DHEA, DHEAS, T, androstenedione (A4), and the adrenal-specific 11-oxygenated C19 steroids (11oxyandrogens) 11β-hydroxytestosterone (11OHT), 11-ketotestosterone (11KT), 11β-hydroxyandrostenedione (11OHA4) and 11-ketoandrostenedione (11KA4) using liquid chromatography-tandem mass spectrometry in morning serum obtained from 100 pre- (age 20-40 years) and 100 post-menopausal (age ≥ 60 years) women...
February 8, 2019: Journal of Clinical Endocrinology and Metabolism
Michael W O'Reilly, Connar Sj Westgate, Catherine Hornby, Hannah Botfield, Angela E Taylor, Keira Markey, James L Mitchell, William J Scotton, Susan P Mollan, Andreas Yiangou, Carl Jenkinson, Lorna C Gilligan, Mark Sherlock, James Gibney, Jeremy W Tomlinson, Gareth G Lavery, David J Hodson, Wiebke Arlt, Alexandra J Sinclair
Idiopathic intracranial hypertension (IIH) is a condition of unknown etiology, characterized by elevated intracranial pressure frequently manifesting with chronic headaches and visual loss. Similar to polycystic ovary syndrome (PCOS), IIH predominantly affects obese women of reproductive age. In this study, we comprehensively examined the systemic and cerebrospinal fluid (CSF) androgen metabolome in women with IIH in comparison to sex-, body mass index- and age-matched control groups with either simple obesity and PCOS, i...
February 12, 2019: JCI Insight
Sebastian Findeklee, Panagiotis Sklavounos, Lisa Stotz, Romina M Sima, Iulian Iordache, Carolin Spüntrup, Mariz Kasoha, Fabinshy Thangarajah, Erich-Franz Solomayer, Julia C Radosa
PURPOSE: Primary and secondary sterility have become an issue of increasing importance due to demographic and social changes in society. Data regarding the association between female androgen levels and the probability of successful conception after fertility treatment are sparse and contradictive. This study was designed to assess this clinical question. METHODS: In this retrospective single-center cohort study concentrations of androgens androstenedione, dehydroepiandrosteronsulfat (DHEAS) and testosterone (ng/ml) were investigated in the serum of patients presenting for sterility at the department of reproductive medicine of Saarland University hospital Homburg between January 2015 and December 2017...
February 8, 2019: Archives of Gynecology and Obstetrics
Xiuling Zhou, Yang Zhang, Yanbing Shen, Xiao Zhang, Shuangping Xu, Zhihua Shang, Menglei Xia, Min Wang
The bioprocess for producing androstenedione (AD) from phytosterols by using Mycobacterium neoaurum is hindered by nicotinamide adenine dinucleotides (NAD+ and NADH) ratio imbalance, insoluble substrate, and lengthy biotransformation period. This study aims to improve the efficiency of AD production through a combined application of cofactor, solvent, and fermentation engineering technologies. Through the enhanced type II NADH dehydrogenase (NDH-II), the NAD+ /NADH ratio and ATP levels increased; the release of reactive oxygen species decreased by 42...
January 31, 2019: Bioresource Technology
Michele Iovino, Tullio Messana, Emanuela Iovino, Giovanni De Pergola, Edoardo Guastamacchia, Vito Angelo Giagulli, Vincenzo Triggiani
In this review, we analyzed the role played by brain areas, neurohormones and neurotransmitters in the regulation of emotional and sexual behavior. We speculate that a common brain pathway is involved in these two behaviors. Hippocampus seems to control the signals coming from the external environment, while the amygdale and the hypothalamus control the response to social stimuli. Stimulation of amygdala increase sexual performace, while it triggers violent emotional responses. Stimulation of the hypothalamus causes reactions of violent anger and increases sexual activity...
January 31, 2019: Endocrine, Metabolic & Immune Disorders Drug Targets
Efstathios Katharopoulos, Kay Sauter, Amit V Pandey, Christa E Flück
Androgens are steroid hormones essential for human male and female development. Steroid reductases 5α (SRD5 As) are key enzymes in androgen biosynthesis. Mutations in the human SRD5 A2 are known to cause loss-of-function and severe 46,XY undervirilization. Gain-of-function variants have been suggested in androgen excess syndromes, but have not been found so far. Therefore we searched for gain-of-function mutations in the human SRD5 A2 gene which might explain hyperandrogenic disorders such as the polycystic ovary syndrome, premature adrenarche and prostate cancer...
January 28, 2019: Journal of Steroid Biochemistry and Molecular Biology
Eva Asselmann, Hanna Kische, Robin Haring, Johannes Hertel, Carsten-Oliver Schmidt, Matthias Nauck, Katja Beesdo-Baum, Hans-Jörgen Grabe, Christiane A Pané-Farré
BACKGROUND: Findings on associations of androgens and sex hormone-binding globulin (SHBG) with anxiety and depressive disorders in the general population remain inconclusive. METHODS: We used data of n = 993 men and n = 980 women from the Study of Health in Pomerania (SHIP, a prospective-longitudinal general population study from northeastern Germany). Immunoassay-measured serum concentrations of total testosterone, androstenedione and SHBG were assessed when participants were aged 20-80...
February 15, 2019: Journal of Affective Disorders
Silvia Baggio, Rossana Di Paola, Stefano Zaffagnini, Stefania Fino, Ricciarda Raffaelli, Massimo Franchi
Background To develop a diagnostic assessment tool, using clinical, biochemical and sonographic markers, to help clinicians in the differential diagnosis of functional oligomenorrhea (FO) and endocrine-metabolic oligomenorrhea (EMO). Methods Sixty-two adolescents with oligomenorrhea without evident hormonal imbalances or severe energy deficit were selected. They were divided into two groups (EMO and FO) and they all underwent the following assessment: physical examination (height, weight, presence of hirsutism or acne), blood exams and transabdominal ultrasonography...
February 25, 2019: Journal of Pediatric Endocrinology & Metabolism: JPEM
M Dziekonski, A Zmijewska, W Czelejewska, P Wojtacha, S Okrasa
Steroid hormones play an important role in the regulation of cyclic changes in the uterus and preparation of intrauterine environment for the egg fertilization, embryo implantation and maintenance of pregnancy. Their secretion by porcine uterus has been demonstrated. The present study aimed to establish the effect of opioid receptors (μ, δ and κ) activation by selective agonists (DAMGO, DPLPE and U 50.488, respectively) on in vitro secretion of steroid hormones (during 6-h and 24-h incubations) by the endometrial explants of gilts on days 2 - 3, 10 - 11, 12 - 3, 15 - 16, 18 - 20 of the estrous cycle and 10 - 11, 12 - 13, 15 - 16 of pregnancy...
October 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
Ken Goeury, Sung Vo Duy, Gabriel Munoz, Michèle Prévost, Sébastien Sauvé
The list of endocrine disrupting compounds (EDCs) defined under U.S. EPA Method 539 was recently expanded to include additional hormones and bisphenol A (BPA). Here, we validated a fast and robust alternative method compliant with Method 539.1 requirements in diverse water matrixes (i.e., ultra-pure water, tap water, surface water, and wastewater influent and effluent). Automated large volume injection solid phase extraction (SPE) coupled on-line to ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was investigated for this purpose...
January 8, 2019: Journal of Chromatography. A
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