keyword
https://read.qxmd.com/read/38530212/prevalence-of-intestinal-trichomonads-in-captive-non-human-primates-in-china
#1
JOURNAL ARTICLE
Ping-Ping Ma, Yang Zou, Wen-Jie Mu, Yue-Yue Zhang, Ya-Qi Li, Zhong-Li Liu, Long Zhang, Li-Xian Chen, Guo-Hua Liu, Shuai Wang
Trichomonads are protozoan symbionts with the capacity to infect vertebrates including humans and non-human primates (NHPs), sometimes with pathogenic effects. However, their diversity and prevalence in NHPs in China are poorly understood. A total of 533 fecal samples were collected from captive NHPs in Yunnan Province, China, of which 461 samples from Macaca fascicularis and 72 from Macaca mulatta. Trichomonadidae species were identified using PCR amplification of the ITS-1/5.8S/ITS-2 sequences. The overall prevalence of trichomonads in NHPs was determined to be 11...
2024: Parasite: Journal de la Société Française de Parasitologie
https://read.qxmd.com/read/38518169/effect-of-phytochemical-compounds-on-trichomonas-tenax-an-oral-protozoan
#2
JOURNAL ARTICLE
Melissa K Stuart, Hannah C Motes, Deborah A Hudman
Trichomonas tenax is an oral protozoan with an estimated global pooled prevalence of 17% in the human population.1 Observational studies have demonstrated a significant statistical correlation between oral colonization by T. tenax and the progression of periodontal disease.2 Proposed pathogenic mechanisms for this protozoan include the production of tissue-damaging enzymes, induction of apoptosis in human cells, and dysbiosis of the oral microbiome.3 In patients for whom metronidazole (MTZ) is contraindicated, phytochemicals may offer a viable alternative for controlling T...
March 22, 2024: Alternative Therapies in Health and Medicine
https://read.qxmd.com/read/38487378/the-parabasal-filaments-of-trichomonas-vaginalis-a-new-filament-and-observations-using-0-8%C3%A2-nm-resolution-scanning-electron-microscopy
#3
JOURNAL ARTICLE
Sharmila Fiama das Neves Ortiz, Raphael Verdan, Gustavo Miranda Rocha, Kildare Miranda, Marlene Benchimol
Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common nonviral sexually transmitted infection worldwide, with an estimated 260 million new cases annually. T. vaginalis contains organelles common to all eukaryotic cells, uncommon cell structures such as hydrogenosomes, and a complex and elaborate cytoskeleton constituting the mastigont system. The mastigont system is mainly formed by several proteinaceous structures associated with basal bodies, the pelta-axostylar complex made of microtubules, and striated filaments named the costa and the parabasal filaments (PFs)...
June 2024: Journal of structural biology: X
https://read.qxmd.com/read/38459096/enhancing-parasitic-organism-detection-in-microscopy-images-through-deep-learning-and-fine-tuned-optimizer
#4
JOURNAL ARTICLE
Yogesh Kumar, Pertik Garg, Manu Raj Moudgil, Rupinder Singh, Marcin Woźniak, Jana Shafi, Muhammad Fazal Ijaz
Parasitic organisms pose a major global health threat, mainly in regions that lack advanced medical facilities. Early and accurate detection of parasitic organisms is vital to saving lives. Deep learning models have uplifted the medical sector by providing promising results in diagnosing, detecting, and classifying diseases. This paper explores the role of deep learning techniques in detecting and classifying various parasitic organisms. The research works on a dataset consisting of 34,298 samples of parasites such as Toxoplasma Gondii, Trypanosome, Plasmodium, Leishmania, Babesia, and Trichomonad along with host cells like red blood cells and white blood cells...
March 8, 2024: Scientific Reports
https://read.qxmd.com/read/38372108/correlation-analysis-of-vaginal-flora-and-immune-function-th1-th2-imbalance-in-women-with-high-risk-hpv-infections-in-the-female-reproductive-tract
#5
JOURNAL ARTICLE
Jingjing Zheng, Rongwei Zhao, Chunxia Deng
The purpose of this study was to analyze the correlation between vaginal flora and immune function Type 1 helper T cells/Type 2 helper T cells imbalance in females having HPV infections at high risk within the female reproductive tract. We selected 150 female patients who visited our hospital for reproductive tract inflammation between March 2019 and March 2021. They were divided into high-risk HPV-positive and high-risk HPV-negative groups according to the results of the HPV tests. Vaginal flora composition, density, diversity, and Th1/Th2 immune cell cytokine expression were assessed, and their correlations were analyzed...
January 31, 2024: Cellular and Molecular Biology
https://read.qxmd.com/read/38366179/commensal-protist-tritrichomonas-musculus-exhibits-a-dynamic-life-cycle-that-induces-extensive-remodeling-of-the-gut-microbiota
#6
JOURNAL ARTICLE
Ana Popovic, Eric Yixiao Cao, Joanna Han, Nirvana Nursimulu, Eliza V C Alves-Ferreira, Kyle Burrows, Andrea Kennard, Noor Alsmadi, Michael E Grigg, Arthur Mortha, John Parkinson
Commensal protists and gut bacterial communities exhibit complex relationships, mediated at least in part through host immunity. To improve our understanding of this tripartite interplay, we investigated community and functional dynamics between the murine protist Tritrichomonas musculus (Tmu) and intestinal bacteria in healthy and B cell-deficient mice. We identified dramatic, protist-driven remodeling of resident microbiome growth and activities, in parallel with Tmu functional changes, which were accelerated in the absence of B cells...
February 5, 2024: ISME Journal
https://read.qxmd.com/read/38318165/clinical-implications-of-trichomonads-detected-in-bronchoalveolar-fluid-by-metagenomic-next-generation-sequencing-a-multicenter-retrospective-study
#7
JOURNAL ARTICLE
Juan Jiang, Yuanyuan Li, Qiong Wang, Huihui Zeng, Wei Yang, Yanhao Wu, Wenzhong Peng, Pinhua Pan, Chengping Hu, Pengbo Deng
BACKGROUND: Pulmonary trichomoniasis is considered a neglected disease due to failures in recognizing it, stemming from insensitive microbial methods and a lack of specific clinical features. This study aims to analyze the clinical implications of trichomonads detected in bronchoalveolar lavage fluid (BALF) by metagenomic next-generation sequencing (mNGS). METHODS: This multicenter retrospective study included patients diagnosed with pneumonia, admitted to three tertiary hospitals in China from July 2018 to September 2022, with trichomonads detected in BALF through mNGS...
2024: Frontiers in Cellular and Infection Microbiology
https://read.qxmd.com/read/38223622/correlation-between-vaginal-flora-and-cervical-immune-function-of-human-papilloma-virus-infected-patients-with-cervical-cancer
#8
JOURNAL ARTICLE
Qiang Wang, Wenmin Qin, Wei Gao, Kai Zhao, Xiaohong Pan, Xiaofei Jiang, Juan Zhao
BACKGROUND: To analyse the correlation between vaginal flora and cervical immune function of HPV-infected patients with cervical cancer. METHODS: Six hundred females with genital tract infections treated in Xuzhou Hospital of Traditional Chinese Medicine from January 2014 to December 2016 were selected and divided into a high-risk HPV group (n=246) and a control group (n=354). The vaginal flora and human T lymphocyte subsets (CD3+, CD4+, CD8+) were detected. Multivariate logistic regression analysis was performed to explore the risk factors for HPV infection...
June 2023: African Health Sciences
https://read.qxmd.com/read/37998756/development-of-an-lfd-rpa-assay-for-rapid-detection-of-pentatrichomonas-hominis-infection-in-dogs
#9
JOURNAL ARTICLE
Yao Rong, Xichen Zhang, Xuejiao Chen, Jianhua Li, Pengtao Gong, Xiaocen Wang, Xin Li, Xu Zhang, Taotao Yue, Hongbo Zhang, Xiaofei Zhou, Nan Zhang
Pentatrichomonas hominis is a trichomonad protozoan that infects the cecum and colon of humans and other mammals. It is a zoonotic pathogen that causes diarrhea in both animals and humans. As companion animals, dogs infected with P. hominis pose a risk of transmitting it to humans. Current methods, such as direct smears and polymerase chain reaction (PCR), used for P. hominis detection have limitations, including low detection rates and the need for specialized equipment. Therefore, there is an urgent need to develop rapid, sensitive, and simple detection methods for clinical application...
November 17, 2023: Current Issues in Molecular Biology
https://read.qxmd.com/read/37902329/-lophomonas-as-a-respiratory-pathogen-jumping-the-gun
#10
REVIEW
Abhishek Mewara, Gillian H Gile, Blaine Mathison, Huan Zhao, Bobbi Pritt, Richard S Bradbury
Human infections with the protozoan Lophomonas have been increasingly reported in the medical literature over the past three decades. Initial reports were based on microscopic identification of the purported pathogen in respiratory specimens. Later, a polymerase chain reaction (PCR) was developed to detect Lophomonas blattarum , following which there has been a significant increase in reports. In this minireview, we thoroughly examine the published reports of Lophomonas infection to evaluate its potential role as a human pathogen...
October 30, 2023: Journal of Clinical Microbiology
https://read.qxmd.com/read/37816433/patterns-of-antibiotic-resistance-of-mycoplasma-hominis-endosymbiont-of-trichomonas-vaginalis-and-the-influence-of-bacterial-intracellular-location-on-drugs-susceptibility
#11
JOURNAL ARTICLE
Valentina Margarita, Gavino Carboni, Nicia Diaz, Paola Rappelli, Pier Luigi Fiori
OBJECTIVES: Mycoplasma hominis, an opportunistic pathogen of the human lower urogenital tract, can survive and replicate within the protozoan Trichomonas vaginalis, establishing an endosymbiotic relationship. The intracellular location may provide a means for the bacteria to evade the immune system and protection from antimicrobial activities. Our aim was to investigate the influence of the endosymbiotic association of M. hominis with trichomonad cells on bacterial antibiotic susceptibility...
October 8, 2023: Journal of Global Antimicrobial Resistance
https://read.qxmd.com/read/37802179/expansion-microscopy-of-trichomonads
#12
JOURNAL ARTICLE
Paula Terra Bandeira, Sharmila Fiama das Neves Ortiz, Marlene Benchimol, Wanderley de Souza
Light microscopy has significantly advanced in recent decades, especially concerning the increased resolution obtained in fluorescence images. Here we present the Expansion microscopy (ExM) technique in two parasites, Trichomonas vaginalis, and Tritrichomonas foetus, which significantly improved the localization of distinct proteins closely associated with cytoskeleton by immunofluorescence microscopy. The ExM techniques have been used in various cell types, tissues, and other protist parasites. It requires the embedment of the samples in a swellable gel that is highly hydrophilic...
October 4, 2023: Experimental Parasitology
https://read.qxmd.com/read/37764084/genetic-variability-of-the-internal-transcribed-spacer-and-pyruvate-ferredoxin-oxidoreductase-partial-gene-of-trichomonas-vaginalis-from-female-patients
#13
JOURNAL ARTICLE
Fernando Martinez-Hernandez, Fabiola Sanchez-Aguillon, Joel Martinez-Ocaña, Nelly Raquel Gonzalez-Arenas, Mirza Romero-Valdovinos, Eduardo Lopez-Escamilla, Pablo Maravilla, Guiehdani Villalobos
In the present study, we evaluated the genetic variability of the internal transcribed spacer (ITS) region and the pyruvate:ferredoxin oxidoreductase ( pfor ) A gene of Trichomonas vaginalis from female patients and its possible implications in the host-parasite relationship. Phylogenetic and genetics of populations analyses were performed by analyzing sequences of the ITS region and partial pfor A gene of clinical samples with T. vaginalis , as previously documented. Alignments of protein sequences and prediction of three-dimensional structure were also performed...
September 5, 2023: Microorganisms
https://read.qxmd.com/read/37758477/the-use-of-hypochlorous-acid-test-and-removal-and-cross-fostering-to-eradicate-mouse-pathogens-in-an-animal-facility
#14
JOURNAL ARTICLE
Hsin-Yi Shih, Ying-Chun Chen, Ching-Fong Liao
Hypochlorous acid (HOCl), used as a liquid or a fog, has broad antimicrobial and deodorizing effects. Our facility was thefirst in Taiwan that was built with a system to supply stabilized, biosafe HOCl solution (50 ppm available chlorine concentration,pH 6) into a new animal barrier facility that housed genetically modified mice. The HOCl system creates an extremelyclean environment that allows us to raise mice in static, filter-top cages and to handle them on open tables without the needfor biologic safety cabinets (BSC)...
September 27, 2023: Journal of the American Association for Laboratory Animal Science: JAALAS
https://read.qxmd.com/read/37300520/fine-structure-and-molecular-characterization-of-two-new-parabasalid-species-that-naturally-colonize-laboratory-mice-tritrichomonas-musculus-and-tritrichomonas-casperi
#15
JOURNAL ARTICLE
Leila Tuzlak, Eliza V C Alves-Ferreira, Cindi L Schwartz, Andrea Kennard, Jacqueline M Leung, Christina Shehata, Michael E Grigg
Tritrichomonas muris is a common flagellated protist isolated from the cecum of wild rodents. This commensal protist has been shown previously to alter immune phenotypes in laboratory mice. Other trichomonads, referred to as Tritrichomonas musculis and Tritrichomonas rainier, also naturally colonize laboratory mice and cause immune alterations. This report formally describes two new trichomonads, Tritrichomonas musculus n. sp., and Tritrichomonas casperi n. sp., at the ultrastructural and molecular level. These two protists were isolated from laboratory mice, and were differentiated by their size and the structure of their undulating membrane and posterior flagellum...
June 10, 2023: Journal of Eukaryotic Microbiology
https://read.qxmd.com/read/37291797/true-molecular-phylogenetic-position-of-the-cockroach-gut-commensal-lophomonas-blattarum-lophomonadida-parabasalia
#16
JOURNAL ARTICLE
LeAnn Nguyen, Stephen J Taerum, Daniel E Jasso-Selles, Claudio H Slamovits, Jeffrey D Silberman, Gillian H Gile
No abstract text is available yet for this article.
June 8, 2023: Journal of Eukaryotic Microbiology
https://read.qxmd.com/read/37111472/the-non-canonical-iron-responsive-element-of-ire-tvcp12-hairpin-structure-at-the-3-utr-of-trichomonas-vaginalis-tvcp12-mrna-that-binds-tvhsp70-and-tvactn-3-can-regulate-mrna-stability-and-amount-of-protein
#17
JOURNAL ARTICLE
Claudia R León-Sicairos, Elisa E Figueroa-Angulo, Jaeson S Calla-Choque, Rossana Arroyo
Trichomonas vaginalis is one of the most common sexually transmitted parasites in humans. This protozoan has high iron requirements for growth, metabolism, and virulence. However, iron concentrations also differentially modulate T. vaginalis gene expression as in the genes encoding cysteine proteinases TvCP4 and TvCP12. Our goal was to identify the regulatory mechanism mediating the upregulation of tvcp12 under iron-restricted (IR) conditions. Here, we showed by RT-PCR, Western blot, and immunocytochemistry assays that IR conditions increase mRNA stability and amount of TvCP12...
April 12, 2023: Pathogens
https://read.qxmd.com/read/37011833/iron-restriction-increases-the-expression-of-a-cytotoxic-cysteine-proteinase-tvcp2-by-a-novel-mechanism-of-tvcp2-mrna-alternative-polyadenylation-in-trichomonas-vaginalis
#18
JOURNAL ARTICLE
Luis Alberto Rivera-Rivas, Rossana Arroyo
Trichomonas vaginalis TvCP2 (TVAG_057000) is a cytotoxic cysteine proteinase (CP) expressed under iron-limited conditions. This work aimed to identify one of the mechanisms of tvcp2 gene expression regulation by iron at the posttranscriptional level. We checked tvcp2 mRNA stability under both iron-restricted (IR) and high iron (HI) conditions in the presence of actinomycin D. Greater stability of the tvcp2 mRNA under the IR than in HI conditions was observed, as expected. In silico analysis of the 3' regulatory region showed the presence of two putative polyadenylation signals in the tvcp2 transcript...
April 1, 2023: Biochimica et Biophysica Acta. Gene Regulatory Mechanisms
https://read.qxmd.com/read/36789430/ultrastructural-identification-and-molecular-characterization-of-two-new-parabasalid-species-that-naturally-colonize-laboratory-mice-tritrichomonas-musculus-and-tritrichomonas-casperi
#19
Leila Tuzlak, Eliza V C Alves-Ferreira, Andrea Kennard, Christina Shehata, Cindi L Schwartz, Michael E Grigg
Tritrichomonas muris is a flagellated protist isolated from the cecum of wild mice in the Czech Republic. This commensal protist has been shown previously to alter immune phenotypes in laboratory mice. Other trichomonads, previously referred to as Tritrichomonas musculis and Tritrichomonas rainier , also naturally colonize laboratory mice and cause immune alterations. This report formally describes two new trichomonads, Tritrichomonas musculus n. sp., and Tritrichomonas casperi n. sp., at the ultrastructural and molecular level...
January 21, 2023: bioRxiv
https://read.qxmd.com/read/36708914/drug-susceptibility-testing-for-oxygen-dependent-and-oxygen-independent-resistance-phenotypes-in-trichomonads
#20
JOURNAL ARTICLE
Alexander Y F Lam, Ernest Lacey, Vesna De Petra, Deborah A Williamson, Jan Šlapeta, Aaron R Jex, Samantha J Emery-Corbin
Trichomonas vaginalis is the most prevalent, non-viral sexually transmitted human infection, causing 170 million cases of trichomoniasis annually. Since the 1950s, treatment has relied on 5-nitroimidazoles (5NIs), leading to increasing drug resistance. A similar drug resistance problem is present in the veterinary pathogen, Tritrichomonas foetus. There are currently no agreed standards for defining 5NI resistance, due in part to two distinct oxygen-dependent ("aerobic") and oxygen-independent ("anaerobic") resistance phenotypes...
January 25, 2023: International Journal for Parasitology
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