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Maria Cuvertoret-Sanz, Christiane Weissenbacher-Lang, Madeleine Lunardi, René Brunthaler, Mònica Coma, Herbert Weissenböck, Joaquim Segalés
Enteric disease in pigs is usually of multifactorial etiology, including infectious and non-infectious factors. In many cases of endemic diarrhea in weaner-to-finisher pigs, the combination of 2 or more microorganisms leads to aggravation of intestinal lesions and, consequently, clinical signs. We autopsied a 4-mo-old fattening pig with diarrhea and diagnosed severe fibrinonecrotizing typhlocolitis. Numerous spiral-shaped bacteria and amoeba-like PAS-positive protozoa were observed in the cecal and colonic mucosa and submucosa...
February 9, 2019: Journal of Veterinary Diagnostic Investigation
Wen-Chao Li, Kai Wang, Yan Li, Li-Ping Zhao, Yi Xiao, You-Fang Gu
Background: In pigs, several different trichomonad species such as Tritrichomonas foetus , Tetratrichomonas buttreyi, and Pentatrichomonas hominis have been described as inhabiting the digestive tract. However, little information is available on the epidemiology of these neglected parasites in the Chinese pig population. Methods: The prevalence of T. suis , T. buttreyi and P. hominis among 500 fecal specimens from pigs at seven pigs farms in Anhui Province in China between Oct and Dec 2014, was determined by PCR and DNA sequence analysis of the small subunit ribosomal RNA (SSU rRNA) genes...
October 2018: Iranian Journal of Parasitology
Kuo-Yang Huang, Ruei-Min Chen, Hsin-Chung Lin, Wei-Hung Cheng, Hsin-An Lin, Wei-Ning Lin, Po-Jung Huang, Cheng-Hsun Chiu, Petrus Tang
BACKGROUND: Autophagy has been shown to be involved in the pathogenesis of several protists, offering prospects for the developments of new drugs targeting autophagy. However, there is no evidence illustrating functional autophagy in the deep-branching trichomonads. The human parasitic protist Trichomonas vaginalis has been predicted to possess reduced autophagic machinery, with only autophagy-related protein 8 (Atg8) conjugation system required for autophagosome formation. METHODS: The recombinant protein of TvAtg8 (rTvAtg8) and the polyclonal antibody against rTvAtg8 were generated...
November 22, 2018: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
Monika Dybicz, Konrad Perkowski, Aleksandra Sędzikowska, Wanda Baltaza, Lidia Chomicz
Trichomonas tenax, cosmopolitan flagellate inhabiting human oral cavity, is the etiological agent of oral trichomonosis associated with gingival and periodontium deterioration. Purpose of this studies was to investigate the prevalence of infection with Trichomonas tenax identified by molecular techniques amplifying the region of ITS1-5.8S rRNA-ITS2 specific for T. tenax. The study included 498 persons: diabetic, renal transplant, rheumatoid arthritis patients and the control group. Prevalence of T. tenax in oral cavity was 10...
2018: Annals of Parasitology
Monika Dybicz, Konrad Perkowski, Wanda Baltaza, Marcin Padzik, Aleksandra Sędzikowska, Lidia Chomicz
INTRODUCTION: The protozoan <i>Trichomonas tenax</i> is considered to be a human specific flagellate of the oral cavity, found in humans with poor oral hygiene and advanced periodontal disease. Morphological variability and great similarity between species occurring in humans and animals, complicate the specific identification of trichomonads, using microscopic examination and other standard parasitological techniques. OBJECTIVE: The aim of the study was to search for and identify <i>T...
September 25, 2018: Annals of Agricultural and Environmental Medicine: AAEM
Maria Del Carmen Martínez-Herrero, Fernando González-González, Irene López-Márquez, Francisco Javier García-Peña, Jose Sansano-Maestre, Rafael Alberto Martínez-Díaz, Francisco Ponce-Gordo, Maria Magdalena Garijo-Toledo, María Teresa Gómez-Muñoz
A juvenile Cinereous Vulture ( Aegypius monachus) fledgling was found disorientated on the roof of a building in Madrid City, Spain, in October 2016. A veterinary examination revealed multiple plaques distributed throughout the oropharyngeal cavity. Lesions were located under the tongue and at the choanal slit, hard palate, and esophagus opening and ranged from 2 to 7 mm, coalescing in areas up to 2 cm, with a yellowish color of the surface. Motile trichomonad trophozoites were detected in fresh wet mount smears from the lesions...
September 20, 2018: Journal of Wildlife Diseases
Catherine Bisson, Pierre-Henry Lec, Michel Blique, Nathalie Thilly, Marie Machouart
This study aims to evaluate the prevalence of trichomonads in the subgingival biofilm of patients with periodontitis. Secondarily, the trichomonad presence was related to patient characteristics and periodontal clinical parameters, in order to highlight the factor favoring the development of these protozoans. Subgingival biofilm samples were collected from at least two diseased and one healthy site in 50 patients suffering from periodontitis. Trichomonads were identified using phase contrast microscopy. All patient characteristics and periodontal clinical parameter data were then statistically analyzed...
December 2018: Parasitology Research
Krysta H Rogers, Yvette A Girard, Leslie W Woods, Christine K Johnson
Avian trichomonosis is an upper digestive tract disease of birds typically caused by the protozoan parasite Trichomonas gallinae . In California (U.S.A), trichomonosis is known to cause periodic epidemics in the Pacific Coast band-tailed pigeon ( Patagioenas fasciata monolis ), a migratory upland game bird. We summarize the mortality events that occurred during winter 2014-2015 including the duration, estimated mortality, pathology, and genetic identity of infecting parasites. Increased mortality was reported from locations in 25 counties between November 2014 and June 2015...
December 2018: International Journal for Parasitology. Parasites and Wildlife
Mendel Friedman, Vincent Huang, Quincel Quiambao, Sabrina Noritake, Jenny Liu, Ohkun Kwon, Sirisha Chintalapati, Joseph Young, Carol E Levin, Christina Tam, Luisa W Cheng, Kirkwood M Land
Potato peel, a waste product of the potato processing industry, is high in bioactive compounds. We investigated the in vitro antitrichomonad activity of potato peel powders prepared from commercial Russet, red, purple, and fingerling varieties as well as several known potato components, alkaloids and phenolic compounds, against three pathogenic strains of trichomonads. Trichomonas vaginalis is a sexually transmitted protozoan parasite that causes the human disease trichomoniasis. Two distinct strains of the related Tritrichomonas fetus infect cattle and cats...
August 1, 2018: Journal of Agricultural and Food Chemistry
Amandeep Singh, Grant Fong, Jenny Liu, Yun-Hsuan Wu, Kevin Chang, William Park, Jihwan Kim, Christina Tam, Luisa W Cheng, Kirkwood M Land, Vipan Kumar
In this study, we outline the synthesis of isatin-ferrocenyl chalcone and 1 H -1,2,3-triazole-tethered isatin-ferrocene conjugates along with their antimicrobial evaluation against the human mucosal pathogen Trichomonas vaginalis . The introduction of a triazole ring among the synthesized conjugates improved the activity profiles with most of the compounds in the library, exhibiting 100% growth inhibition in a preliminary susceptibility screen at 100 μM. IC50 determination of the most potent compounds in the set revealed an inhibitory range between 2 and 13 μM...
May 31, 2018: ACS Omega
Diana Belén Sánchez-Rodríguez, Jaime Ortega-López, Rosa Elena Cárdenas-Guerra, Gerardo Reséndiz-Cardiel, Bibiana Chávez-Munguía, Anel Lagunes-Guillen, Rossana Arroyo
Trichomonas vaginalis is a flagellated protist responsible for human trichomoniasis. T. vaginalis has three genes encoding for endogenous cysteine proteinase (CP) inhibitors, known as trichocystatin-1 through trichocystatin-3 (TC-1, TC-2, and TC-3). These inhibitors belong to the cystatin family. In this study, we characterized trichocystatin-3 (TC-3), an endogenous cysteine proteinase (CP) inhibitor of T. vaginalis. TC-3 possesses a signal peptide in the N-terminus and two putative glycosylation sites (typical of family 2, cystatins) but lacks the PW motif and cysteine residues (typical of family 1, stefins)...
September 2018: International Journal of Biochemistry & Cell Biology
Steven T Laing, David Merriam, Barbara C Shock, Sarah Mills, Abbie Spinner, Rachel Reader, Dennis J Hartigan-O'Connor
Idiopathic chronic diarrhea (ICD) is a common ailment affecting captive rhesus macaques ( Macaca mulatta). ICD cases are characterized by diarrhea in the absence of commonly identified diarrheal pathogens and multiple recurrences even after supportive therapy. Histologically, the disease is characterized by lymphoplasmacytic colitis. We identified 35 rhesus macaques euthanized for ICD during a 7-month period and described demographic, clinical, histologic, and immunologic commonalities. We found a trend of historic Campylobacter spp...
September 2018: Veterinary Pathology
Zabiholah Zarei, Mehdi Mohebali, Khadijeh Khanaliha, Eshrat Beigom Kia, Afsaneh Motevalli Haghi, Zahra Heidari, Tahereh Rezaeian, Mostafa Rezaeian
Background: Trichomonas muris is one of the most common protozoa diagnosed in rodents. The trichomonads are generally described as presenting only trophozoite form while pseudocyst is another morphological form of trichomonads identified among gastrointestinal and genitourinary trichomonads. We identified and described different shapes of T. muris pseudocysts and trophozoite in stool samples were collected from rodents including Merinos persicus , Mus musculus and Cricetulus migratorius ...
May 2018: Iranian Journal of Public Health
Malek J Hallinger, Anja Taubert, Carlos Hermosilla, Frank Mutschmann
BACKGROUND: Exotic reptiles such as tortoises, have become increasingly common domestic pets worldwide and are known to host different gastrointestinal parasites. Some of these parasites bear zoonotic potential. In the present survey, we parasitologically examined tortoise faecal samples (n = 1005) from 19 different species held as pets in private German households and German zoological gardens. METHODS: Saline faecal smears were used to generate prevalence data for potentially health-compromising gastrointestinal parasites...
June 18, 2018: Parasites & Vectors
Rosa E Cárdenas-Guerra, Moisés Martínez-Castillo, Jaime Ortega-López, Mineko Shibayama, Rossana Arroyo
The recombinant TvCP4 prepro region (ppTvCP4r) acts as an exogenous inhibitor of cathepsin L-like CPs from Trichomonas vaginalis (Cárdenas-Guerra et al., 2015 [1]). Here, we present the dataset of the trichomonad ppTvCP4r inhibitory effect against the CP proteolytic activities from other microorganisms, such as Naegleria fowleri and Acanthamoeba castellanii free-living amoeba. The proteolytic activity inhibition of total crude extracts (TCEs) of N. fowleri and A. castellanii was determined and recorded using a fluorogenic substrate specific for cathepsin L CPs without or with a ppTvCP4r treatment at different concentrations and pH...
June 2018: Data in Brief
Bethânia Ferreira Bastos, Beatriz Brener, Mariana Alves de Figueiredo, Daniela Leles, Flavya Mendes-de-Almeida
Case series summary: Chronic diarrhea is a common and recurring problem in feline medicine. Intestinal trichomonads have been reported as causative agents of diarrhea in cats. While Pentatrichomonas hominis is considered commensal, Tritrichomonas foetus has been found to cause feline large bowel diarrhea in cats. In our report, two young cats infected with the feline leukemia virus (FeLV) and presenting with chronic diarrhea were identified as having trichomonads in their feces, based on direct examination and fecal culture...
January 2018: JFMS Open Reports
Arim Min, Young Ah Lee, Kyeong Ah Kim, Myeong Heon Shin
Trichomonas vaginalis is a sexually-transmitted protozoan parasite that causes vaginitis and cervicitis. Although mast cell activation is important for provoking tissue inflammation during infection with parasites, information regarding the signaling mechanisms in mast cell activation and T. vaginalis infection is limited. O-linked N-acetylglucosamine (O-GlcNAc) is a post-translational modification of serine and threonine residues that functions as a critical regulator of intracellular signaling, regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA)...
June 2018: Microbes and Infection
C Grunenwald, I Sidor, R Mickley, C Dwyer, R Gerhold
During an outbreak of Wellfleet Bay virus (WFBV) in common eiders ( Somateria mollissima) from the Cape Cod region of Massachusetts, several birds were diagnosed with trichomonosis consisting of multiple trichomonad species. Six birds were examined, with trichomonads found in ceca in four birds and associated typhlitis in three of these four birds. PCR and DNA sequencing utilizing trichomonad-specific primers targeting the ITS1 region of the ribosomal DNA (rDNA) revealed the presence of Tetratrichomonas gallinarum in the gastrointestinal tracts of five birds and Trichomonas spp...
March 2018: Avian Diseases
Chaoqun Yao, Jennifer K Ketzis
The clinical significance and frequency of human infections with trichomonad flagellates in aberrant locations (locations other than the predilection sites) and accidental infections (infections with non-human trichomonads) are unclear. The total number of case reports in the literature is low, with the identification of the infection largely the outcome of investigations for the cause of clinical signs. At least seven species have been identified in either aberrant or accidental infections, with these infections occurring worldwide...
February 1, 2018: Transactions of the Royal Society of Tropical Medicine and Hygiene
María Elizbeth Alvarez-Sánchez, Laura Itzel Quintas-Granados, Laura Isabel Vázquez-Carrillo, Jonathan Puente-Rivera, Alma Villalobos-Osnaya, María Dolores Ponce-Regalado, Minerva Camacho-Nuez
Infection with Trichomonas vaginalis produces a malodorous seropurulent vaginal discharge due to several chemicals, including polyamines. The presence of 1,4-diamino-2-butanone (DAB) reduces the amount of intracellular putrescine by 90%, preventing the cotransport of exogenous spermine. DAB-treated parasites present morphological changes, which are restored by adding exogenous putrescine into the culture medium. However, the effect of polyamines over the trichomonad proteomic profile is unknown. In this study, we used a proteomic approach to analyze the polyamine-depletion and restoration effect by exogenous putrescine on T...
May 2018: Parasitology Research
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