keyword
https://read.qxmd.com/read/17050705/the-orexin-ox1-receptor-regulates-ca2-entry-via-diacylglycerol-activated-channels-in-differentiated-neuroblastoma-cells
#21
COMPARATIVE STUDY
Johnny Näsman, Genevieve Bart, Kim Larsson, Lauri Louhivuori, Hanna Peltonen, Karl E O Akerman
We studied the cellular response to orexin type 1 receptor (OX1R) stimulation in differentiated IMR-32 neuroblastoma cells. In vitro differentiation of IMR-32 cells with 5-bromo-2'-deoxyuridine leads to a neuronal phenotype with long neurite extensions and an upregulation of mainly N-type voltage-gated calcium channels. Transduction of differentiated IMR-32 cells with baculovirus harboring an OX1R-green fluorescent protein cDNA fusion construct resulted in appearance of fluorescence that was confined mainly to the plasma membrane in the cell body and to neurites...
October 18, 2006: Journal of Neuroscience
https://read.qxmd.com/read/16129666/app1-transcription-is-regulated-by-inositol-phosphorylceramide-synthase-1-diacylglycerol-pathway-and-is-controlled-by-atf2-transcription-factor-in-cryptococcus-neoformans
#22
JOURNAL ARTICLE
Lydia Mare, Roberta Iatta, Maria Teresa Montagna, Chiara Luberto, Maurizio Del Poeta
Inositol-phosphorylceramide synthase 1 (Ipc1) is a fungal-specific enzyme that regulates the level of two bioactive molecules, phytoceramide and diacylglycerol (DAG). In previous studies, we demonstrated that Ipc1 regulates the expression of the antiphagocytic protein 1 (App1), a novel fungal factor involved in pathogenicity of Cryptococcus neoformans. Here, we investigated the molecular mechanism by which Ipc1 regulates App1. To this end, the APP1 promoter was fused to the firefly luciferase gene in the C...
October 28, 2005: Journal of Biological Chemistry
https://read.qxmd.com/read/16097951/induction-of-apoptosis-by-lipophilic-activators-of-ctp-phosphocholine-cytidylyltransferase-alpha-cctalpha
#23
JOURNAL ARTICLE
Thomas A Lagace, Neale D Ridgway
Farnesol (FOH) inhibits the CDP-choline pathway for PtdCho (phosphatidylcholine) synthesis, an activity that is involved in subsequent induction of apoptosis. Interestingly, the rate-limiting enzyme in this pathway, CCTalpha (CTP:phosphocholine cytidylyltransferase alpha), is rapidly activated, cleaved by caspases and exported from the nucleus during FOH-induced apoptosis. The purpose of the present study was to determine how CCTalpha activity and PtdCho synthesis contributed to induction of apoptosis by FOH and oleyl alcohol...
December 15, 2005: Biochemical Journal
https://read.qxmd.com/read/15632189/translocation-of-diacylglycerol-kinase-theta-from-cytosol-to-plasma-membrane-in-response-to-activation-of-g-protein-coupled-receptors-and-protein-kinase-c
#24
JOURNAL ARTICLE
Jürgen van Baal, John de Widt, Nullin Divecha, Wim J van Blitterswijk
Diacylglycerol kinase (DGK) phosphorylates the second messenger diacylglycerol (DAG) to phosphatidic acid. We previously identified DGK as one of nine mammalian DGK isoforms and reported on its regulation by interaction with RhoA and by translocation to the plasma membrane in response to noradrenaline. Here, we have investigated how the localization of DGK, fused to green fluorescent protein, is controlled upon activation of G protein-coupled receptors in A431 cells. Extracellular ATP, bradykinin, or thrombin induced DGK translocation from the cytoplasm to the plasma membrane within 2-6 min...
March 18, 2005: Journal of Biological Chemistry
https://read.qxmd.com/read/15537648/orexin-a-induced-ca2-entry-evidence-for-involvement-of-trpc-channels-and-protein-kinase-c-regulation
#25
JOURNAL ARTICLE
Kim P Larsson, Hanna M Peltonen, Genevieve Bart, Lauri M Louhivuori, Annika Penttonen, Miia Antikainen, Jyrki P Kukkonen, Karl E O Akerman
The orexins are peptide transmitters/hormones, which exert stimulatory actions in many types of cells via the G-protein-coupled OX(1) and OX(2) receptors. Our previous results have suggested that low (subnanomolar) concentrations of orexin-A activate Ca(2+) entry, whereas higher concentrations activate phospholipase C, Ca(2+) release, and capacitative Ca(2+) entry. As shown here, the Ca(2+) response to subnanomolar orexin-A concentrations was blocked by activation of protein kinase C by using different approaches (12-O-tetradecanoylphorbol acetate, dioctanoylglycerol, and diacylglycerol kinase inhibition) and protein phosphatase inhibition by calyculin A...
January 21, 2005: Journal of Biological Chemistry
https://read.qxmd.com/read/15292052/cell-density-dependent-expression-of-edg-family-receptors-and-mesangial-cell-proliferation-role-in-lysophosphatidic-acid-mediated-cell-growth
#26
JOURNAL ARTICLE
Yiding Xing, Shobha H Ganji, Jung W Noh, Vaijinath S Kamanna
Lysophosphatidic acid (LPA), a major member of the bioactive lysophospholipids in serum, possesses diverse physiological activities including cell proliferation. Recently, three endothelial differentiation gene (EDG) family receptors, including EDG-2 (LPA1), EDG-4 (LPA2), and EDG-7 (LPA3), have been identified as LPA receptors. The role of LPA and their receptors in mesangial cell physiology is not clearly understood. This study examined the expression profile of EDG receptors as a function of cell density and the participation of EDG receptors in human mesangial cell proliferation by LPA...
December 2004: American Journal of Physiology. Renal Physiology
https://read.qxmd.com/read/15277524/autophosphorylation-suppresses-whereas-kinase-inhibition-augments-the-translocation-of-protein-kinase-calpha-in-response-to-diacylglycerol
#27
JOURNAL ARTICLE
Helena Stensman, Arathi Raghunath, Christer Larsson
We have seen that protein kinase Calpha (PKCalpha) is transiently translocated to the plasma membrane by carbachol stimulation of neuroblastoma cells. This is induced by the Ca2+ increase, and PKCalpha does not respond to diacylglycerol (DAG). The unresponsiveness is dependent on structures in the catalytic domain of PKCalpha. This study was designed to investigate if and how the kinase activity and autophosphorylation are involved in regulating the translocation. PKCalpha enhanced green fluorescent protein translocation was studied in living neuroblastoma cells by confocal microscopy...
September 24, 2004: Journal of Biological Chemistry
https://read.qxmd.com/read/15013437/role-of-phospholipase-d-in-the-activation-of-protein-kinase-d-by-lysophosphatidic-acid
#28
JOURNAL ARTICLE
Yoonseok Kam, John H Exton
Protein kinase D was auto-phosphorylated at Ser916 and trans-phosphorylated at Ser744/Ser748 in Rat-2 fibroblasts treated with lysophosphatidic acid. Both phosphorylations were inhibited by 1-butanol, which blocks phosphatidic acid formation by phospholipase D. The phosphorylations were also reduced in Rat-2 clones with decreased phospholipase D activity. Platelet-derived growth factor-induced protein kinase D phosphorylation showed a similar requirement for phospholipase D, but that induced by 4beta-phorbol 12 myristate 13-acetate did not...
February 27, 2004: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/14653694/coumarin-4-ylmethoxycarbonyls-as-phototriggers-for-alcohols-and-phenols
#29
JOURNAL ARTICLE
Akinobu Z Suzuki, Takayoshi Watanabe, Mika Kawamoto, Keiko Nishiyama, Hirotaka Yamashita, Megumi Ishii, Michiko Iwamura, Toshiaki Furuta
Caged compounds can be used to regulate the spatial and temporal dynamics of signaling molecules in live cells. Photochemical properties of coumarin-4-ylmethoxy carbonates (1a-d) are investigated to construct caged compounds of hydroxy-containing molecules. All the compounds possess desired properties as phototriggers for alcohols and phenols. The 6-bromo-7-hydroxycoumarin-4-ylmethoxycarbonyl (Bhcmoc) group has the highest photochemical efficiency and is applied to make caged compounds of 1,2-dioctanoylglycerol (diC(8)), Tyr-OMe, and adenosine...
December 11, 2003: Organic Letters
https://read.qxmd.com/read/12874449/protein-kinase-c-activation-downregulates-human-organic-anion-transporter-1-mediated-transport-through-carrier-internalization
#30
JOURNAL ARTICLE
Natascha A Wolff, Karen Thies, Nicola Kuhnke, Glen Reid, Björn Friedrich, Florian Lang, Gerhard Burckhardt
Organic anion transport in intact renal proximal tubule cells in animal model systems is downregulated by treatments that activate protein kinase C (PKC). How this downregulation is achieved is not yet known. Stimulation of PKC with sn-1,2-dioctanoylglycerol resulted in strong inhibition of p-aminohippurate transport mediated by the cloned human organic anion transporter 1 (hOAT1) expressed in Xenopus oocytes and HEK293 cells, as well as hOAT1 internalization in both expression systems. The sn-1,2-dioctanoylglycerol-induced transport inhibition was partially prevented by staurosporine...
August 2003: Journal of the American Society of Nephrology: JASN
https://read.qxmd.com/read/12799309/mapk-mediates-pkc-dependent-contraction-of-cat-esophageal-and-lower-esophageal-sphincter-circular-smooth-muscle
#31
JOURNAL ARTICLE
Weibiao Cao, Uy Dong Sohn, Khalil N Bitar, Jose Behar, Piero Biancani, Karen M Harnett
Esophageal (ESO) circular muscle contraction and lower esophageal sphincter (LES) tone are PKC dependent. Because MAPKs may be involved in PKC-dependent contraction, we examined ERK1/ERK2 and p38 MAPKs in ESO and LES. In permeabilized LES muscle cells, ERK1/2 antibodies reduced 1,2-dioctanoylglycerol (DG)- and threshold ACh-induced contraction, which are PKC dependent, but not maximal ACh, which is calmodulin dependent. LES tone was reduced by the ERK1/2 kinase inhibitor PD-98059 and by the p38 MAPK inhibitor SB-203580...
July 2003: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://read.qxmd.com/read/12460119/the-catalytic-domain-limits-the-translocation-of-protein-kinase-c-alpha-in-response-to-increases-in-ca2-and-diacylglycerol
#32
JOURNAL ARTICLE
Arathi Raghunath, Mia Ling, Christer Larsson
Translocation of protein kinase C (PKC) alpha, beta II, delta and epsilon fused to enhanced green fluorescent protein (EGFP) was studied in living neuroblastoma cells by confocal microscopy. Exposure to carbachol elicited transient translocation of PKC alpha-EGFP and beta II-EGFP in most of the cells, PKC delta-EGFP in a few cells and induced sustained translocation of PKC epsilon-EGFP. To monitor levels of Ca(2+) and diacylglycerol and the translocation of PKC in the same cell, the Ca(2+)-sensitive C2 domain, diacylglycerol-sensitive C1 domains and full-length PKC were fused to red, cyan and yellow fluorescent proteins respectively...
March 15, 2003: Biochemical Journal
https://read.qxmd.com/read/12063164/transforming-growth-factor-beta1-regulation-of-growth-zone-chondrocytes-is-mediated-by-multiple-interacting-pathways
#33
JOURNAL ARTICLE
Enrique Rosado, Zvi Schwartz, Victor L Sylvia, David D Dean, Barbara D Boyan
Transforming growth factor beta 1 (TGF-beta1) affects growth plate chondrocytes through Smad-mediated mechanisms and has been shown to increase protein kinase C (PKC). This study determined if PKC mediates the physiological response of rat costochondral growth zone (GC) chondrocytes to TGF-beta1; if the physiological response occurs via type II or type III TGF-beta receptors, and, if so, which receptor mediates the increase in PKC; and the signal transduction pathways involved. Treatment of confluent GC cells with TGF-beta1 stimulated [(3)H]thymidine and [(35)S]sulfate incorporation as well as alkaline phosphatase (ALPase) and PKC specific activities...
June 12, 2002: Biochimica et Biophysica Acta
https://read.qxmd.com/read/11782881/the-antilipolytic-agent-3-5-dimethylpyrazole-inhibits-insulin-release-in-response-to-both-nutrient-secretagogues-and-cyclic-adenosine-monophosphate-agonists-in-isolated-rat-islets
#34
JOURNAL ARTICLE
P Masiello, M Novelli, M Bombara, V Fierabracci, S Vittorini, M Prentki, E Bergamini
This study intended to test the hypothesis that intracellular lipolysis in the pancreatic beta cells is implicated in the regulation of insulin secretion stimulated by nutrient secretagogues or cyclic adenosine monophosphate (cAMP) agonists. Indeed, although lipid signaling molecules were repeatedly reported to influence beta-cell function, the contribution of intracellular triglycerides to the generation of these molecules has remained elusive. Thus, we have studied insulin secretion of isolated rat pancreatic islets in response to various secretagogues in the presence or absence of 3,5-dimethylpyrazole (DMP), a water-soluble and highly effective antilipolytic agent, as previously shown in vivo...
January 2002: Metabolism: Clinical and Experimental
https://read.qxmd.com/read/11607161/diacylglycerols-induce-both-ion-pumping-in-patch-clamped-guard-cell-protoplasts-and-opening-of-intact-stomata
#35
JOURNAL ARTICLE
Y Lee, S M Assmann
Stomatal guard cells in leaves regulate the apertures of microscopic pores through which photosynthetic gas exchange and water vapor loss occur. Environmental signals, including light, high humidity, and low CO2 concentrations, open stomata by increasing the volume of guard cells. Activation of a plasma membrane H+ pump initiates K+ and Cl- influx, accompanied by malate synthesis, resulting in osmotic water flow into the guard cells, a bowing apart of the guard-cell pair, and consequent stomatal opening. Physiological and electrophysiological techniques were employed to investigate the possibility that a second-messenger lipid, 1,2-diacylglycerol, is involved in the transduction of opening stimuli...
March 15, 1991: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/11581251/stimulation-of-lipolysis-and-hormone-sensitive-lipase-via-the-extracellular-signal-regulated-kinase-pathway
#36
JOURNAL ARTICLE
A S Greenberg, W J Shen, K Muliro, S Patel, S C Souza, R A Roth, F B Kraemer
Hormonally stimulated lipolysis occurs by activation of cyclic AMP-dependent protein kinase (PKA) which phosphorylates hormone-sensitive lipase (HSL) and increases adipocyte lipolysis. Evidence suggests that catecholamines not only can activate PKA, but also the mitogen-activated protein kinase pathway and extracellular signal-regulated kinase (ERK). We now demonstrate that two different inhibitors of MEK, the upstream activator of ERK, block catecholamine- and beta(3)-stimulated lipolysis by approximately 30%...
November 30, 2001: Journal of Biological Chemistry
https://read.qxmd.com/read/11502742/protein-kinase-c-regulates-the-phosphorylation-and-cellular-localization-of-occludin
#37
JOURNAL ARTICLE
A Y Andreeva, E Krause, E C Müller, I E Blasig, D I Utepbergenov
Occludin is an integral membrane phosphoprotein specifically associated with tight junctions, contributing to the structure and function of this intercellular seal. Occludin function is thought to be regulated by phosphorylation, but no information is available on the molecular pathways involved. In the present study, the involvement of the protein kinase C pathway in the regulation of the phosphorylation and cellular distribution of occludin has been investigated. Phorbol 12-myristate 13-acetate and 1,2-dioctanoylglycerol induced the rapid phosphorylation of occludin in Madin-Darby canine kidney cells cultured in low extracellular calcium medium with a concomitant translocation of occludin to the regions of cell-cell contact...
October 19, 2001: Journal of Biological Chemistry
https://read.qxmd.com/read/11447027/myosin-light-chain-kinase-and-pkc-dependent-contraction-of-les-and-esophageal-smooth-muscle
#38
JOURNAL ARTICLE
U D Sohn, W Cao, D C Tang, J T Stull, J R Haeberle, C L Wang, K M Harnett, J Behar, P Biancani
In smooth muscle cells enzymatically isolated from circular muscle of the esophagus (ESO) and lower esophageal sphincter (LES), ACh-induced contraction and myosin light chain (MLC) phosphorylation were similar. Contraction and phosphorylation induced by purified MLC kinase (MLCK) were significantly greater in LES than ESO. ACh-induced contraction and MLC phosphorylation were inhibited by calmodulin and MLCK inhibitors in LES and by protein kinase C (PKC) inhibitors in ESO. Contraction of LES and ESO induced by the PKC agonist 1,2-dioctanoylglycerol (DG) was unaffected by MLCK inhibitors...
August 2001: American Journal of Physiology. Gastrointestinal and Liver Physiology
https://read.qxmd.com/read/11384235/an-optimized-protein-kinase-c-activating-diacylglycerol-combining-high-binding-affinity-ki-with-reduced-lipophilicity-log-p
#39
JOURNAL ARTICLE
K Nacro, D M Sigano, S Yan, M C Nicklaus, L L Pearce, N E Lewin, S H Garfield, P M Blumberg, V E Marquez
A small, focused combinatorial library encompassing all possible permutations of acyl branched alkyl chains-small and large, saturated and unsaturated-was generated from the active diacylglycerol enantiomer (S-DAG) to help identify the analogue with the highest binding affinity (lowest Ki) for protein kinase C (PK-C) combined with the minimum lipophilicity (log P). The selected ligand (3B) activated PK-C more effectively than sn-1,2-dioctanoylglycerol (diC8) despite being 1.4 log units more hydrophilic. Compound 3B indeed represents the most potent, hydrophilic DAG ligand to date...
June 7, 2001: Journal of Medicinal Chemistry
https://read.qxmd.com/read/11331416/utp-evokes-noradrenaline-release-from-rat-sympathetic-neurons-by-activation-of-protein-kinase-c
#40
JOURNAL ARTICLE
N Vartian, E Moskvina, T Scholze, U Unterberger, C Allgaier, S Boehm
The pathway involved in UTP-evoked noradrenaline release was investigated in cultures of rat superior cervical ganglia. Northern blots revealed an age-related increase in levels of mRNA for P2Y6 receptors in cultures obtained at postnatal days 1 and 5, respectively, but no change in transcripts for P2Y1 and P2Y2. Likewise, UTP-evoked overflow of previously incorporated [(3)H]noradrenaline was six-fold higher in neurons obtained at postanatal day 5. Various protein kinase C inhibitors diminished UTP-, but not electrically, induced tritium overflow by > 70%, as did down-regulation of protein kinase C by 24 h exposure to phorbol ester...
May 2001: Journal of Neurochemistry
keyword
keyword
169315
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.