Sándor Farkas, Daniel Cioca, József Murányi, Péter Hornyák, Attila Brunyánszki, Patrik Szekér, Eszter Boros, Patrik Horváth, Zoltán Hujber, Gábor Z Rácz, Noémi Nagy, Rebeka Tóth, László Nyitray, Zalán Péterfi
Chlorotoxin, a scorpion venom-derived 36-residue miniprotein, binds to and is taken up selectively by glioblastoma cells. Previous studies provided controversial results concerning target protein(s) of CTX. These included: CLC3 chloride channel, matrix metalloproteinase 2 (MMP-2), regulators of MMP-2, annexin A2 (ANX2) and neuropilin 1 (NRP1). The aim of this study was to clarify which of the proposed binding partners can really interact with CTX using biochemical methods and recombinant proteins. For this purpose, we established two new binding assays based on anchoring the tested proteins to microbeads and quantifying the binding of CTX by flow cytometry...
June 30, 2023: Journal of Biological Chemistry