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chloride channel and vascular

Muhammed Fatih Dogan, Seyfullah Oktay Arslan, Oguzhan Yildiz, Murat Kurtoglu, Ali Parlar
OBJECTIVES: The aim of this study was to investigate the vascular effects and mechanisms of propofol in the human internal mammary artery (IMA). DESIGN: In vitro experimental study. SETTING: The study was conducted in the research laboratory of a pharmacology department. PARTICIPANTS: IMA segments were obtained from 52 patients undergoing coronary artery bypass surgery. INTERVENTIONS: The IMA rings were suspended in isolated organ baths, and the changes in the tension were isometrically recorded...
December 11, 2018: Journal of Cardiothoracic and Vascular Anesthesia
Yu-Tao Guan, Yong Xie, Hui Zhou, Hai-Yan Shi, Yu-Yuan Zhu, Xiao-Lu Zhang, Yi Luan, Xi-Ming Shen, Yang-Ping Chen, Li-Jiang Xu, Zhong-Qiu Lin, Gang Wang
Background: Cervical carcinoma is a major gynecological cancer and causes cancer-related deaths in worldwide, the latent pathogenesis and progress of cervical cancer is still under research. ClC-3 may be an important promoter for aggressive metastasis of malignant tumors. In this research, we explore the ClC-3 expression in cervical carcinoma and its underlying clinical significance, trying to illuminate ClC-3 probable function in the neoplasm malignant behavior, development and prognosis...
2019: Cancer Cell International
Liliqiang Ding, Chenglin Jia, Yong Zhang, Wenjian Wang, Weiliang Zhu, Yu Chen, Teng Zhang
Scutellaria baicalensis Georgi is an extensively used medicinal herb for the treatment of hypertension in traditional Chinese medicine. Baicalin is the most abundant flavone compound present in Scutellaria baicalensis Georgi and endothelium-dependent vascular activities of baicalin have been suggested. However, the pharmacological implications and mechanisms of baicalin under hypertensive conditions remain to be investigated. The current study examined the blood pressure-lowering effect of baicalin in a spontaneously hypertensive rat (SHR) model...
December 24, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Chang Chen, Cong Guo, Jian Gao, Kaifeng Shi, Jingtang Cheng, Jun Zhang, Sha Chen, Yan Liu, An Liu
BACKGROUND: Both Chuanxiong (Ligusticum chuanxiong Hort) and Tianma (Gastrodia elata Blume) have the effects of vasorelaxation and antihypertension. However, the effects of Tianshu Capsule (TSC, composed of Chuanxiong and Tianma in the mass ratio of 4:1) on antihypertensive activity have not been explored. This study aimed to investigate the eff ;ects of TSC on vascular tension and blood pressure in rats and to explore the underlying mechanisms. METHODS: The vasorelaxant effect of TSC was explored on thoracic aortic rings (both intact endothelium and denuded) preincubated with phenylephrine (Phe) or potassium chloride (KCL)...
December 21, 2018: Biomedicine & Pharmacotherapy
Rana Attalla, Erin Puersten, Nidhi Jain, P Ravi Selvaganapathy
One of the primary focuses in recent years in tissue engineering has been the fabrication and integration of vascular structures into artificial tissue constructs. However, most available methodologies lack the ability to create multi-layered concentric conduits inside natural extracellular matrices (ECMs) and gels that replicate more accurately the hierarchical architecture of biological blood vessels. In this work, we present a new microfluidic nozzle design capable of multi-axial extrusion in order to 3D print and pattern bi- and tri-layered hollow channel structures...
December 11, 2018: Biofabrication
Christopher M Hearon, Jennifer C Richards, Mathew L Racine, Gary J Luckasen, Dennis G Larson, Frank A Dinenno
KEY POINTS: In humans, the vasodilatory response to skeletal muscle contraction is mediated in part by activation of inwardly rectifying potassium (KIR ) channels. Evidence from animal models suggest that KIR channels serve as electrical amplifiers of endothelium-dependent hyperpolarization (EDH). We found that skeletal muscle contraction amplifies vasodilatation to the endothelium-dependent agonist acetylcholine (ACh), whereas there was no change in the vasodilatory response to sodium nitroprusside, an endothelium-independent nitric oxide donor...
December 1, 2018: Journal of Physiology
A H Ahmed, I M Maulood
Urotensin-II (U-II), the most potent vasoconstrictor that has recently been recognized as a new candidate in cardiovascular dysfunction, might exert vasoconstriction through, at least partially, potassium channels that are predominant in both endothelial and vascular smooth muscle cells (VSMCs). The present study was designed to evaluate the roles of potassium channels in vascular responses to U-II in intact and mercury induced endothelial dysfunction in rat aorta. The study involved pre-incubation of rat aortic rings with potassium channels blockers: charybdotoxin (chtx), tetraethylammonium (TEA), barium chloride (BaCl2 ), glibenclamide, 4-aminopyridine (4-AP) and clotrimazole...
2018: Iranian Journal of Veterinary Research
Zhao Liu, Mi Chen, Lin-Ka Xie, Ting Liu, Zhen-Wei Zou, Yong Li, Peng Chen, Xin Peng, Charlie Ma, Wen-Jie Zhang, Pin-Dong Li
Calcium activated Chloride Channel A4 (CLCA4), as a tumor suppressor, was reported to contribute to the progression of several malignant tumors, yet little is known about the significance of CLCA4 in invasion and prognosis of hepatocellular carcinoma (HCC). CLCA4 expression was negatively correlated with tumor size, vascular invasion and TNM stage. Kaplan-Meier analysis showed that CLCA4 was an independent predictor for overall survival (OS) and time to recurrence (TTR). In addition, CLCA4 status could act as prognostic predictor in different risk of subgroups...
October 11, 2018: Aging
Sonal Singh, Zhiying Wang, Mohamed H Shahin, Taimour Y Langaee, Yan Gong, Stephen T Turner, Arlene B Chapman, John G Gums, Caitrin W McDonough, Kent R Bailey, Amber L Beitelshees, Rhonda M Cooper-DeHoff, Steve Scherer, Eric Boerwinkle, Julie A Johnson
Chromosome 12q15 was identified in Genetic Epidemiology of Response Assessment (GERA) and replicated in Pharmacogenomic Evaluation of Antihypertensive Responses (PEAR) for its association with blood pressure (BP) response to hydrochlorothiazide (HCTZ). However, the functional variant is unknown and we aimed to identify the likely functional variants through targeted sequencing. The chromosome 12q15 region was sequenced in 397 best and worst responders to HCTZ in PEAR (N=199) and GERA (N=198) hypertensive study participants...
November 2018: Pharmacogenetics and Genomics
Sara Emilia Lima Tolouei, Cleide Adriane Signor Tirloni, Rhanany Alan Calloi Palozi, Maysa Isernhagen Schaedler, Lucas Pires Guarnier, Aniely Oliveira Silva, Valter Paes de Almeida, Jane Manfron Budel, Roosevelt Isaias Carvalho Souza, Ariany Carvalho Dos Santos, Vanessa Samúdio Dos Santos, Denise Brentan Silva, Paulo Roberto Dalsenter, Arquimedes Gasparotto Junior
ETHNOPHARMACOLOGICAL RELEVANCE: Celosia argentea L. (Amaranthaceae), popularly known as "crista de galo", is used in folk medicine due to its diuretic and hypotensive effects. However, there are no reports in the literature regarding its pharmacological effects on the cardiovascular system as well as no data proving the safety of this species. AIM: To perform a detailed ethnopharmacological investigation of the ethanol soluble fraction from C. argentea (ESCA) using male and female Wistar rats...
September 21, 2018: Journal of Ethnopharmacology
Michał Majewski, Adam Jurgoński, Bartosz Fotschki, Jerzy Juśkiewicz
We investigated the potential effects of monosodium glutamate (MSG)-induced obesity with regards to nitric oxide and prostanoid production, as well as potassium channel function, in rat thoracic arteries. Newborn male Wistar rats were injected intraperitoneally with typically reported MSG (4.0 mg/g) once daily for 4 consecutive days. At 90 days postnatal, the rats were sacrificed and the thoracic aortas were evaluated for vascular responses and for prostanoid production. Nitric oxide was studied with calcium ionophore (A23187), acetylcholine (ACh) and sodium nitroprusside (SNP)...
September 19, 2018: Toxicology and Applied Pharmacology
Tzu-Ju Chen, Hong-Lin He, Yow-Ling Shiue, Ching-Chieh Yang, Li-Ching Lin, Yu-Feng Tian, Shang-Hung Chen
Background: Concurrent chemoradiotherapy (CCRT) has now become the standard of treatments for advanced rectal cancer before surgery. To search the biological molecules with prognostic and therapeutic potential of CCRT could be beneficial for these patients. Recently, aberrant expression of chloride channels has been linked to radio-resistance in glioblastoma; however, its clinical implication has not been well-studied in rectal cancers. Therefore, we examined the clinical significance of targetable drivers associated with chloride channel activity in patients with rectal cancer receiving CCRT...
2018: International Journal of Medical Sciences
Kay-Pong Yip, Lavanya Balasubramanian, Chen Kan, Lei Wang, Ruisheng Liu, Luisa Ribeiro-Silva, James S K Sham
Myogenic contraction of renal arterioles is an important regulatory mechanism for renal blood flow autoregulation. We have previously demonstrated that integrin-mediated mechanical force increases the occurrence of Ca2+ sparks in freshly isolated renal vascular smooth muscle cells (VSMCs). To further test whether generation of Ca2+ sparks is a downstream signal of mechanotransduction in pressure induced myogenic constriction, the relationship between Ca2+ spark frequency and transmural perfusion pressure was investigated in intact VSMCs of pressurized rat afferent arterioles...
August 8, 2018: American Journal of Physiology. Renal Physiology
Gabrielle Gauvin-Rossignol, Philippe Legros, Jean Ruel, Marc-André Fortin, André Bégin-Drolet
Alginate-based hydrogels are widely used for the development of biomedical scaffolds in regenerative medicine. The use of sugar glass as a sacrificial template for fluidic channels fabrication within alginate scaffolds remains a challenge because of the premature dissolution of sugar by the water contained in the alginate as well as the relatively slow internal gelation rate of the alginate. Here, a new and simple method, based on a sugar glass fugitive ink loaded with calcium chloride to build sacrificial molds, is presented...
July 2018: Heliyon
Lin-Yan Huang, Yu-Jie Li, Peng-Peng Li, Hong-Chun Li, Ping Ma
Our previous study found that ClC-3 chloride channel functioned differently in the vascular and intestinal inflammation, the loss of ClC-3 reduced vascular inflammation but exacerbated intestinal inflammation. To furtherly clarify the role of ClC-3 chloride channels in systemic inflammation, we used LPS-induced endotoxemia model to investigate the response of wild-type and ClC-3 knockout mice to systemic inflammation. The results showed that in the LPS-induced endotoxemia model, the mortality of mice with ClC-3 deletion was significantly higher than that of wild-type mice...
September 2018: Cell Biology International
Kirsty MacRae, Kylie Connolly, Rebecca Vella, Andrew Fenning
PURPOSE: Cardiovascular disease is the leading cause of mortality globally. Epicatechin has previously been shown to improve vascular responses and possess cardioprotective properties. However, the mechanisms underpinning these cardiotropic outcomes remain unknown. The aim of this study was to further identify epicatechin's mechanism of action in the cardiovasculature. METHODS: The effects of epicatechin on isolated rat conduit arteries, resistance vessels and cardiac electrophysiology were investigated on resting tension and precontracted vessels and cardiac action potential parameters, both in the presence and in the absence of various antagonists...
May 10, 2018: European Journal of Nutrition
Kemal Gokhan Ulusoy, Erkan Kaya, Kubilay Karabacak, Melik Seyrek, İbrahim Duvan, Vedat Yildirim, Oguzhan Yildiz
The vascular actions and mechanisms of taurine were investigated in the isolated human radial artery (RA). RA rings were suspended in isolated organ baths and tension was recorded isometrically. First, a precontraction was achieved by adding potassium chloride (KCl, 45 mM) or serotonin (5-hydroxytryptamine, 5-HT, 30 µM) to organ baths. When the precontractions were stable, taurine (20, 40, 80 mM) was added cumulatively. Antagonistic effect of taurine on calcium chloride (10 µM to 10 mM)-induced contractions was investigated...
November 2017: Korean Journal of Physiology & Pharmacology
Simone V Lip, Anne Marijn van der Graaf, Marjon J Wiegman, Sicco A Scherjon, Mark V Boekschoten, Torsten Plösch, Marijke M Faas
Normal pregnancy requires adaptations of the maternal vasculature. During preeclampsia these adaptations are not well established, which may be related to maternal hypertension and proteinuria. The effects of preeclampsia on the maternal vasculature are not yet fully understood. We aimed to evaluate gene expression in aortas of pregnant rats with experimental preeclampsia using a genome wide microarray. Aortas were isolated from pregnant Wistar outbred rats with low-dose LPS-induced preeclampsia (ExpPE), healthy pregnant (Pr), non-pregnant and low-dose LPS-infused non-pregnant rats...
November 1, 2017: Scientific Reports
Armond Daci, Burim Neziri, Shaip Krasniqi, Raif Cavolli, Rame Alaj, Giuseppe Danilo Norata, Giangiacomo Beretta
The goal of this study was to test the effects of bioactive phenylpropanoid dibenzylbutyrolactone lignan arctigenin (ATG) in vascular tone. Human bypass graft vessel, from a saphenous vein (SV), were set up in organ bath system and contracted with potassium chloride (KCl, 40mM). Two concentration-response curves of noradrenaline (NE) (10nM-100μM) separated with an incubation period of 30min without (Control) or with ATG (3-100μM) were established. Inhibitors of nitric oxide, prostaglandins, K+ related channels or calcium influx were used to delineate the molecular mechanisms beyond ATG effects...
September 5, 2017: European Journal of Pharmacology
J Donovan, P S Wong, R E Roberts, M J Garle, S P H Alexander, W R Dunn, V Ralevic
Hypoxia-induced coronary artery vasodilatation protects the heart by increasing blood flow under ischemic conditions, however its mechanism is not fully elucidated. Hydrogen sulfide (H2 S) is reported to be an oxygen sensor/transducer in the vasculature. The present study aimed to identify and characterise the role of H2 S in the hypoxic response of the coronary artery, and to define the H2 S synthetic enzymes involved. Immunoblotting and immunohistochemistry showed expression of all three H2 S-producing enzymes, cystathionine-β-synthase (CBS), cystathionine-γ-lyase (CSE) and 3-mercaptopyruvate sulfurtransferase (MPST), in porcine coronary artery...
August 2017: Vascular Pharmacology
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