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JOURNAL ARTICLE
Ekaterina N Kochkina, Elizaveta E Kopylova, Olga A Rogachevskaja, Nina P Kovalenko, Natalia V Kabanova, Polina D Kotova, Marina F Bystrova, Stanislav S Kolesnikov
In mammals, three genes encode IP3 receptors (IP3 Rs), which are involved in agonist-induced Ca2+ signaling in cells of apparently all types. Using the CRISPR/Cas9 approach for disruption of two out of three IP3 R genes in HEK-293 cells, we generated three monoclonal cell lines, IP3R1-HEK, IP3R2-HEK, and IP3R3-HEK, with the single functional isoform, IP3 R1, IP3 R2, and IP3 R3, respectively. All engineered cells responded to ACh with Ca2+ transients in an "all-or-nothing" manner, suggesting that each IP3 R isotype was capable of mediating CICR...
March 22, 2024: Cells
#2
JOURNAL ARTICLE
Chenyan Zhang, Bowen Liu, Jiaxing Sheng, Jia Wang, Weijie Zhu, Chen Xie, Xuan Zhou, Yuxin Zhang, Qinghai Meng, Yu Li
After myocardial infarction (MI), there is a notable disruption in cellular calcium ion homeostasis and mitochondrial function, which is believed to be intricately linked to endoplasmic reticulum (ER) stress. This research endeavors to elucidate the involvement of glucose regulated protein 75 (GRP75) in post-MI calcium ion homeostasis and mitochondrial function. In MI rats, symptoms of myocardial injury were accompanied by an increase in the activation of ER stress. Moreover, in oxygen-glucose deprivation (OGD)-induced cardiomyocytes, it was confirmed that inhibiting ER stress exacerbated intracellular Ca2+ disruption and cell apoptosis...
March 23, 2024: European Journal of Pharmacology
#3
REVIEW
Meiqin Hu, Xinghua Feng, Qiang Liu, Siyu Liu, Fangqian Huang, Haoxing Xu
The endomembrane system consists of organellar membranes in the biosynthetic pathway: endoplasmic reticulum (ER), Golgi apparatus, and secretory vesicles, as well as those in the degradative pathway: early endosomes, macropinosomes, phagosomes, autophagosomes, late endosomes, and lysosomes. These endomembrane organelles/vesicles work together to synthesize, modify, package, transport, and degrade proteins, carbohydrates, and lipids, regulating the balance between cellular anabolism and catabolism. Large ion concentration gradients exist across endomembranes - Ca2+ gradients for most endomembrane organelles and H+ gradients for the acidic compartments...
March 7, 2024: Physiological Reviews
#4
JOURNAL ARTICLE
Miyesaier Abudureyimu, Xuanming Luo, Lingling Jiang, Xuejuan Jin, Cuizhen Pan, Wei Yu, Junbo Ge, Yingmei Zhang, Jun Ren
AIMS: Heart failure with preserved ejection fraction (HFpEF) is a devastating health issue although limited knowledge is available for its pathogenesis and therapeutics. Given the perceived involvement of mitochondrial dysfunction in HFpEF, this study was designed to examine the role of mitochondrial dynamics in the etiology of HFpEF. METHOD AND RESULTS: Adult mice were placed on a high fat diet plus l-NAME in drinking water ('two-hit' challenge to mimic obesity and hypertension) for 15 consecutive weeks...
April 2024: Redox Biology
#5
JOURNAL ARTICLE
Rui Shi, Zhenhua Liu, Huan Yue, Man Li, Simin Liu, Dema De, Runjing Li, Yunan Chen, Shuli Cheng, Xiaoming Gu, Min Jia, Jun Li, Juan Li, Shumiao Zhang, Na Feng, Rong Fan, Feng Fu, Yali Liu, Mingge Ding, Jianming Pei
INTRODUCTION: There is a high morbidity and mortality rate in mechanical trauma (MT)-induced hepatic injury. Currently, the molecular mechanisms underlying liver MT are largely unclear. Exploring the underlying mechanisms and developing safe and effective medicines to alleviate MT-induced hepatic injury is an urgent requirement. The aim of this study was to reveal the role of mitochondria-associated ER membranes (MAMs) in post-traumatic liver injury, and ascertain whether melatonin protects against MT-induced hepatic injury by regulating MAMs...
February 2, 2024: Cellular & Molecular Biology Letters
#6
REVIEW
Rui Zhang, Xu-Xu Wang, Jia-Fei Xie, Ting-Ting Yao, Qian-Wen Guo, Qi Wang, Zhen Ding, Jin-Peng Zhang, Mei-Rong Zhang, Li-Chun Xu
A widely used type II pyrethroid pesticide cypermethrin (CYP) is one of endocrine disrupting chemicals (EDCs) with anti-androgenic activity to induce male reproductive toxicology. However, the mechanisms have not been fully elucidated. This study was to explore the effects of CYP on apoptosis of mouse Sertoli cells (TM4) and the roles of endoplasmic reticulum (ER)-mitochondria coupling involving 1,4,5-trisphosphate receptor type1-glucose-regulated protein 75-voltage-dependent anion channel 1 (IP3R1-GRP75-VDAC1)...
January 29, 2024: Reproductive Toxicology
#7
JOURNAL ARTICLE
Zeyu Li, Ou Hu, Suowen Xu, Chenjia Lin, Wenjing Yu, Dinghu Ma, Jing Lu, Peiqing Liu
Mitochondria are energy-producing organelles that are mobile and harbor dynamic network structures. Although mitochondria and endoplasmic reticulum (ER) play distinct cellular roles, they are physically connected to maintain functional homeostasis. Abnormal changes in this interaction have been linked to pathological states, including cardiac hypertrophy. However, the exact regulatory molecules and mechanisms are yet to be elucidated. Here, we report that ATPase family AAA-domain containing protein 3A (ATAD3A) is an essential regulator of ER-mitochondria interplay within the mitochondria-associated membrane (MAM)...
2024: International Journal of Biological Sciences
#8
JOURNAL ARTICLE
Miriam E Hurley, Shihab S Shah, Thomas M D Sheard, Hannah M Kirton, Derek S Steele, Nikita Gamper, Izzy Jayasinghe
Coordinated events of calcium (Ca2+ ) released from the endoplasmic reticulum (ER) are key second messengers in excitable cells. In pain-sensing dorsal root ganglion (DRG) neurons, these events can be observed as Ca2+ sparks, produced by a combination of ryanodine receptors (RyR) and inositol 1,4,5-triphosphate receptors (IP3R1). These microscopic signals offer the neuronal cells with a possible means of modulating the subplasmalemmal Ca2+ handling, initiating vesicular exocytosis. With super-resolution dSTORM and expansion microscopies, we visualised the nanoscale distributions of both RyR and IP3R1 that featured loosely organised clusters in the subplasmalemmal regions of cultured rat DRG somata...
December 23, 2023: Cells
#9
REVIEW
Karl Swann
Mammalian egg activation at fertilization is triggered by a long-lasting series of increases in cytosolic Ca2+ concentration. These Ca2+ oscillations are due to the production of InsP3 within the egg and the subsequent release of Ca2+ from the endoplasmic reticulum into the cytosol. The generation of InsP3 is initiated by the diffusion of sperm-specific phospholipase Czeta1 (PLCζ) into the egg after gamete fusion. PLCζ enables a positive feedback loop of InsP3 production and Ca2+ release which then stimulates further InsP3 production...
December 10, 2023: Cells
#10
JOURNAL ARTICLE
Hiroki Akizawa, Emily M Lopes, Rafael A Fissore
Changes in the intracellular concentration of free calcium (Ca2+ ) underpin egg activation and initiation of development in animals and plants. In mammals, the Ca2+ release is periodical, known as Ca2+ oscillations, and mediated by the type 1 inositol 1,4,5-trisphosphate receptor (IP3 R1). Another divalent cation, zinc (Zn2+ ), increases exponentially during oocyte maturation and is vital for meiotic transitions, arrests, and polyspermy prevention. It is unknown if these pivotal cations interplay during fertilization...
December 15, 2023: ELife
#11
JOURNAL ARTICLE
Jie Wei, Jintao Liu, Huan Wang, Kai Wen, Xiuye Ni, Yilong Lin, Jingru Huang, Xiang You, Zhao Lei, Juan Li, Heqing Shen, Yi Lin
Nonalcoholic steatohepatitis (NASH) is multifactorial that lifestyle, genetic, and environmental factors contribute to its onset and progression, thereby posing a challenge for therapeutic intervention. Nanoplastic (NP) is emerged as a novel environmental metabolism disruptor but the etiopathogenesis remains largely unknown. In this study, C57BL/6 J mice were fed with normal chow diet (NCD) and high-fat diet (HFD) containing 70 nm polystyrene microspheres (NP). We found that dietary-derived NP adsorbed proteins and agglomerated during the in vivo transportation, enabling diet-induced hepatic steatosis to NASH...
November 30, 2023: Journal of Hazardous Materials
#12
JOURNAL ARTICLE
Yuhan Lin, Rui Liang, Kairong Xie, Tingting Ma, Jigui Zhang, Tao Xu, Aimei Wang, Shuangyue Liu
Puerarin (PUE), a flavonoid derivative with vasodilatory effects found in the traditional Chinese medicine kudzu, has anti-sensorineural hearing loss properties. However, the mechanism of its protective effect against ototoxicity is not well understood. In this study, we used in vitro and in vivo methods to investigate the protective mechanism of puerarin against cisplatin (CDDP)-induced ototoxicity. We established an ototoxicity model of CDDP in BALB/c mice and assessed the degree of hearing loss and cochlear cell damage...
December 1, 2023: Biochemical Pharmacology
#13
REVIEW
Sarah S Albalawi, Ahmed Aljabri, Mohannad Alshibani, Mohammed M Al-Gayyar
Nonalcoholic fatty liver disease (NAFLD) is a prevalent and complex condition that affects millions of people globally. It occurs when fat, primarily triglycerides, accumulates in liver cells, leading to inflammation and damage. Calcium, an essential mineral, is involved in various physiological processes, including the regeneration process following liver injury. The endoplasmic reticulum (ER), a complex organelle involved in protein synthesis and lipid metabolism, regulates intracellular calcium levels. Dysregulation of this process can lead to calcium overload, oxidative stress, and cellular damage, all of which are hallmarks of NAFLD...
November 2023: Curēus
#14
JOURNAL ARTICLE
Jussi Pekka Tolonen, Ricardo Parolin Schnekenberg, Simon McGowan, David Sims, Meriel McEntagart, Frances Elmslie, Debbie Shears, Helen Stewart, George K Tofaris, Tabib Dabir, Patrick J Morrison, Diana Johnson, Marios Hadjivassiliou, Sian Ellard, Charles Shaw-Smith, Anna Znaczko, Abhijit Dixit, Mohnish Suri, Ajoy Sarkar, Rachel E Harrison, Gabriela Jones, Henry Houlden, Giorgia Ceravolo, Joanna Jarvis, Jonathan Williams, Morag E Shanks, Penny Clouston, Julia Rankin, Lubov Blumkin, Tally Lerman-Sagie, Penina Ponger, Salmo Raskin, Katariina Granath, Johanna Uusimaa, Hector Conti, Emma McCann, Shelagh Joss, Alexander J M Blakes, Kay Metcalfe, Helen Kingston, Marta Bertoli, Rachel Kneen, Sally Ann Lynch, Inmaculada Martínez Albaladejo, Austen Peter Moore, Wendy D Jones, Esther B E Becker, Andrea H Németh
BACKGROUND: The ITPR1 gene encodes the inositol 1,4,5-trisphosphate (IP3 ) receptor type 1 (IP3 R1), a critical player in cerebellar intracellular calcium signaling. Pathogenic missense variants in ITPR1 cause congenital spinocerebellar ataxia type 29 (SCA29), Gillespie syndrome (GLSP), and severe pontine/cerebellar hypoplasia. The pathophysiological basis of the different phenotypes is poorly understood. OBJECTIVES: We aimed to identify novel SCA29 and GLSP cases to define core phenotypes, describe the spectrum of missense variation across ITPR1, standardize the ITPR1 variant nomenclature, and investigate disease progression in relation to cerebellar atrophy...
January 2024: Movement Disorders: Official Journal of the Movement Disorder Society
#15
JOURNAL ARTICLE
Barış Yıldız, Ramazan Demirel, Hatice Beşeren Havadar, Gülden Yıldız, Cem Öziç, Nadide Nabil Kamiloğlu, Özkan Özden
Sigma-1 receptor (SIG1R) is a chaperone that modulates inositol 1,4,5-trisphosphate receptor type1 (IP3 R1) calcium (Ca2+ ) channels on the endoplasmic reticulum. Therefore, SIG1R functions as an indirect regulator of Ca2+ and acts as an apoptosis modulator. Increased expression of SIG1R is associated with poor prognosis in breast cancers (BC), and SIG1R antagonists like BD1047 induce apoptosis. As a heavy metal, cadmium (Cd2+ ) is competitive with Ca2+ due to its physicochemical similarities and may trigger apoptosis at low concentrations...
November 9, 2023: Biological Trace Element Research
#16
JOURNAL ARTICLE
Scott D Zawieja, Grace A Pea, Sarah E Broyhill, Advaya Patro, Karen H Bromert, Min Li, Charles E Norton, Jorge A Castorena-Gonzalez, Edward J Hancock, Christopher D Bertram, Michael J Davis
Pressure-dependent chronotropy of murine lymphatic collecting vessels relies on the activation of the Ca2+-activated chloride channel encoded by Anoctamin 1 (Ano1) in lymphatic muscle cells. Genetic ablation or pharmacological inhibition of ANO1 results in a significant reduction in basal contraction frequency and essentially complete loss of pressure-dependent frequency modulation by decreasing the rate of the diastolic depolarization phase of the ionic pacemaker in lymphatic muscle cells (LMCs). Oscillating Ca2+ release from sarcoendoplasmic reticulum Ca2+ channels has been hypothesized to drive ANO1 activity during diastole, but the source of Ca2+ for ANO1 activation in smooth muscle remains unclear...
December 4, 2023: Journal of General Physiology
#17
JOURNAL ARTICLE
Rui-Feng Fan, Xue-Wei Chen, Han Cui, Hong-Yu Fu, Wan-Xue Xu, Jiu-Zhi Li, Hai Lin
Skeletal muscle satellite cells (SMSCs), known as muscle stem cells, play an important role in muscle embryonic development, post-birth growth, and regeneration after injury. Selenoprotein K (SELENOK), an endoplasmic reticulum (ER) resident selenoprotein, is known to regulate calcium ion (Ca2+ ) flux and ER stress (ERS). SELENOK deficiency is involved in dietary selenium deficiency-induced muscle injury, but the regulatory mechanisms of SELENOK in SMSCs development remain poorly explored in chicken. Here, we established a SELENOK deficient model to explore the role of SELENOK in SMSCs...
August 22, 2023: Poultry Science
#18
JOURNAL ARTICLE
Yan Li, Lingpeng Zhu, Meng-Xia Cai, Zi-Li Wang, Miao Zhuang, Cheng-Ye Tan, Tian-Hua Xie, Yong Yao, Ting-Ting Wei
Diabetic retinopathy (DR) is a serious and relatively under-recognized complication of diabetes. Müller glial cells extend throughout the retina and play vital roles in maintaining retinal homeostasis. Previous studies have demonstrated that TGR5, a member of the bile acid-activated GPCR family, could ameliorate DR. However, the role of TGR5 in regulating Müller cell function and the underlying mechanism remains to be ascertained. To address this, high glucose (HG)-treated human Müller cells and streptozotocin-treated Sprague-Dawley rats were used in the study...
September 1, 2023: Cell Death & Disease
#19
JOURNAL ARTICLE
Irina X Zhang, Andrea Hermann, Juan Leon, Sivakumar Jeyarajan, Anoop Arunagiri, Peter Arvan, Patrick Gilon, Leslie S Satin
Pancreatic beta cells maintain glucose homeostasis by secreting pulses of insulin in response to a rise in plasma glucose. Pulsatile insulin secretion occurs as a result of glucose-induced oscillations in beta-cell cytosolic Ca2+ . The endoplasmic reticulum (ER) helps regulate beta-cell cytosolic Ca2+ , and ER stress can lead to ER Ca2+ reduction, beta-cell dysfunction, and an increased risk of type 2 diabetes. However, the mechanistic effects of ER stress on individual calcium channels is not well understood...
July 17, 2023: Journal of Biological Chemistry
#20
JOURNAL ARTICLE
Mahesh Ramalingam, Sujeong Jang, Jinsu Hwang, Boeun Kim, Hyong-Ho Cho, Eungpil Kim, Han-Seong Jeong
Mesenchymal stem cells (MSCs) have therapeutic effects on neurodegenerative diseases (NDDs) known by their secreted molecules, referred to as the "secretome". The mitochondrial complex I inhibitor, rotenone (ROT), reproduces α-synuclein (α-syn) aggregation seen in Parkinson's disease (PD). In this present study, we examined the neuroprotective effects of the secretome from neural-induced human adipose tissue-derived stem cells (NI-ADSC-SM) during ROT toxicity in SH-SY5Y cells. Exposure to ROT significantly impaired the mitophagy by increased LRRK2, mitochondrial fission, and endoplasmic reticulum (ER) stress (ERS)...
March 15, 2023: International Journal of Molecular Sciences
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