keyword
https://read.qxmd.com/read/37907479/hops-corvet-and-newly-identified-hybrid-tethering-complexes-contribute-differentially-towards-multiple-modes-of-endocytosis
#21
JOURNAL ARTICLE
Seigo Terawaki, Filipp Vasilev, Takahito Moriwaki, Takanobu Otomo
Vesicular transport driven by membrane trafficking systems conserved in eukaryotes is critical to cellular functionality and homeostasis. It is known that homotypic fusion and vacuole protein sorting (HOPS) and class C core endosomal vacuole tethering (CORVET) interact with Rab-GTPases and SNARE proteins to regulate vesicle transport, fusion, and maturation in autophagy and endocytosis pathways. In this study, we identified two novel "Hybrid" tethering complexes in mammalian cells in which one of the subunits of HOPS or CORVET is replaced with the subunit from the other...
October 31, 2023: Scientific Reports
https://read.qxmd.com/read/37844085/a-rab6-to-rab11-transition-is-required-for-dense-core-granule-and-exosome-biogenesis-in-drosophila-secondary-cells
#22
JOURNAL ARTICLE
Adam Wells, Cláudia C Mendes, Felix Castellanos, Phoebe Mountain, Tia Wright, S Mark Wainwright, M Irina Stefana, Adrian L Harris, Deborah C I Goberdhan, Clive Wilson
Secretory cells in glands and the nervous system frequently package and store proteins destined for regulated secretion in dense-core granules (DCGs), which disperse when released from the cell surface. Despite the relevance of this dynamic process to diseases such as diabetes and human neurodegenerative disorders, our mechanistic understanding is relatively limited, because of the lack of good cell models to follow the nanoscale events involved. Here, we employ the prostate-like secondary cells (SCs) of the Drosophila male accessory gland to dissect the cell biology and genetics of DCG biogenesis...
October 2023: PLoS Genetics
https://read.qxmd.com/read/37802980/rab41-mediated-escrt-machinery-repairs-membrane-rupture-by-a-bacterial-toxin-in-xenophagy
#23
JOURNAL ARTICLE
Takashi Nozawa, Hirotaka Toh, Junpei Iibushi, Kohei Kogai, Atsuko Minowa-Nozawa, Junko Satoh, Shinji Ito, Kazunori Murase, Ichiro Nakagawa
Xenophagy, a type of selective autophagy, is a bactericidal membrane trafficking that targets cytosolic bacterial pathogens, but the membrane homeostatic system to cope with bacterial infection in xenophagy is not known. Here, we show that the endosomal sorting complexes required for transport (ESCRT) machinery is needed to maintain homeostasis of xenophagolysosomes damaged by a bacterial toxin, which is regulated through the TOM1L2-Rab41 pathway that recruits AAA-ATPase VPS4. We screened Rab GTPases and identified Rab41 as critical for maintaining the acidification of xenophagolysosomes...
October 6, 2023: Nature Communications
https://read.qxmd.com/read/37773952/sifa-sumoylation-governs-salmonella-typhimurium-intracellular-survival-via-modulation-of-lysosomal-function
#24
JOURNAL ARTICLE
Hridya Chandrasekhar, Gayatree Mohapatra, Kirti Kajal, Mukesh Singh, Kshitiz Walia, Sarika Rana, Navneet Kaur, Sheetal Sharma, Amit Tuli, Prasenjit Das, Chittur V Srikanth
One of the mechanisms shaping the pathophysiology during the infection of enteric pathogen Salmonella Typhimurium is host PTM machinery utilization by the pathogen encoded effectors. Salmonella Typhimurium (S. Tm) during infection in host cells thrives in a vacuolated compartment, Salmonella containing vacuole (SCV), which sequentially acquires host endosomal and lysosomal markers. Long tubular structures, called as Salmonella induced filaments (SIFs), are further generated by S. Tm, which are known to be required for SCV's nutrient acquisition, membran maintenance and stability...
September 29, 2023: PLoS Pathogens
https://read.qxmd.com/read/37766277/attachment-entry-and-intracellular-trafficking-of-classical-swine-fever-virus
#25
REVIEW
Xin Guo, Maolin Zhang, Xiaomin Liu, Yannan Zhang, Chongyang Wang, Yidi Guo
Classical swine fever virus (CSFV), which is a positive-sense, single-stranded RNA virus with an envelope, is a member of the Pestivirus genus in the Flaviviridae family. CSFV causes a severe and highly contagious disease in pigs and is prevalent worldwide, threatening the pig farming industry. The detailed mechanisms of the CSFV life cycle have been reported, but are still limited. Some receptors and attachment factors of CSFV, including heparan sulfate (HS), laminin receptor (LamR), complement regulatory protein (CD46), MER tyrosine kinase (MERTK), disintegrin, and metalloproteinase domain-containing protein 17 (ADAM17), were identified...
September 3, 2023: Viruses
https://read.qxmd.com/read/37759799/regulation-of-endosomal-trafficking-by-rab7-and-its-effectors-in-neurons-clues-from-charcot-marie-tooth-2b-disease
#26
REVIEW
Ryan J Mulligan, Bettina Winckler
Intracellular endosomal trafficking controls the balance between protein degradation and synthesis, i.e., proteostasis, but also many of the cellular signaling pathways that emanate from activated growth factor receptors after endocytosis. Endosomal trafficking, sorting, and motility are coordinated by the activity of small GTPases, including Rab proteins, whose function as molecular switches direct activity at endosomal membranes through effector proteins. Rab7 is particularly important in the coordination of the degradative functions of the pathway...
September 16, 2023: Biomolecules
https://read.qxmd.com/read/37703297/noncanonical-rab9a-action-supports-retromer-mediated-endosomal-exit-of-human-papillomavirus-during-virus-entry
#27
JOURNAL ARTICLE
Jeongjoon Choi, Daniel DiMaio
Rab GTPases play key roles in controlling intracellular vesicular transport. GTP-bound Rab proteins support vesicle trafficking. Here, we report that, unlike cellular protein cargos, retromer-mediated delivery of human papillomaviruses (HPV) into the retrograde transport pathway during virus entry is inhibited by Rab9a in its GTP-bound form. Knockdown of Rab9a inhibits HPV entry by modulating the HPV-retromer interaction and impairing retromer-mediated endosome-to-Golgi transport of the incoming virus, resulting in the accumulation of HPV in the endosome...
September 13, 2023: PLoS Pathogens
https://read.qxmd.com/read/37662169/rab7-gtpases-as-coordinators-of-plant-endomembrane-traffic
#28
REVIEW
Cecilia Rodriguez-Furlan, Rita Borna, Oliver Betz
The ras gene from rat brain (RAB) family of small GTPases is highly conserved among eukaryotes and regulates endomembrane trafficking pathways. RAB7, in particular, has been linked to various processes involved in regulating endocytic and autophagic pathways. Plants have several copies of RAB7 proteins that reflect the intricacy of their endomembrane transport systems. RAB7 activity regulates different pathways of endomembrane trafficking in plants: (1) endocytic traffic to the vacuole; (2) biosynthetic traffic to the vacuole; and (3) recycling from the late endosome to the secretory pathway...
2023: Frontiers in Plant Science
https://read.qxmd.com/read/37610835/the-snare-associated-protein-sft2-functions-in-imh1-mediated-snare-recycling-transport-upon-er-stress
#29
JOURNAL ARTICLE
Chun-Chi Lai, Wan-Yun Chiu, Yan-Ting Chen, Chia-Lu Wu, Fang-Jen S Lee
Vesicular trafficking involving SNARE proteins play a crucial role in the delivery of cargo to the target membrane. Arf-like protein 1 (Arl1) is an important regulator of the endosomal trans -Golgi network (TGN) and secretory trafficking. In yeast, ER stress-enhances Arl1 activation and Golgin Imh1 recruitment to the late-Golgi. Although Arl1 and Imh1 are critical for GARP-mediated endosomal SNARE-recycling transport in response to ER stress, their downstream effectors are unknown. Here, we report that the SNARE-associated protein Sft2 acts downstream of the Arl1-Imh1 axis to regulate SNARE recycling upon ER stress...
October 1, 2023: Molecular Biology of the Cell
https://read.qxmd.com/read/37604243/casr-links-endocytic-and-secretory-pathways-via-madd-a-rab11a-effector-that-activates-rab27b
#30
JOURNAL ARTICLE
Jorge Eduardo Del-Río-Robles, Janik Adriana Tomás-Morales, Cesar Zavala-Barrera, Alejandro Castillo-Kauil, Irving García-Jiménez, José Vázquez-Prado, Guadalupe Reyes-Cruz
Calcium sensing receptor (CaSR), a class C GPCR, regulates essential secretory pathways, involving communication between endocytic and secretory Rab GTPases, via still to be fully defined molecular mechanisms. To address how communication between endocytic and secretory vesicles occurs, we hypothesized that CaSR activates endocytic Rab11A-dependent effector pathways acting upstream of Rab27B-regulated secretion. We found that Rab11A is critical to promote Rab27B-dependent secretion of chemotactic and inflammatory factors, including IL-8, CCL2/MCP-1, and IL1-β, in response to CaSR stimulation...
August 19, 2023: Cellular Signalling
https://read.qxmd.com/read/37494394/vasodilators-mobilize-sk3-channels-in-endothelial-cells-to-produce-arterial-relaxation
#31
JOURNAL ARTICLE
Dieniffer Peixoto-Neves, Shambhu Yadav, Charles E MacKay, Ulrich C Mbiakop, Alejandro Mata-Daboin, M Dennis Leo, Jonathan H Jaggar
Endothelial cells (ECs) line the lumen of all blood vessels and regulate functions, including contractility. Physiological stimuli, such as acetylcholine (ACh) and intravascular flow, activate transient receptor potential vanilloid 4 (TRPV4) channels, which stimulate small (SK3)- and intermediate (IK)-conductance Ca2+ -activated potassium channels in ECs to produce vasodilation. Whether physiological vasodilators also modulate the surface abundance of these ion channels in ECs to elicit functional responses is unclear...
August 2023: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/37463208/regulatory-sites-in-the-mon1-ccz1-complex-control-rab5-to-rab7-transition-and-endosome-maturation
#32
JOURNAL ARTICLE
Ann-Christin Borchers, Maren Janz, Jan-Hannes Schäfer, Arne Moeller, Daniel Kümmel, Achim Paululat, Christian Ungermann, Lars Langemeyer
Maturation from early to late endosomes depends on the exchange of their marker proteins Rab5 to Rab7. This requires Rab7 activation by its specific guanine nucleotide exchange factor (GEF) Mon1-Ccz1. Efficient GEF activity of this complex on membranes depends on Rab5, thus driving Rab-GTPase exchange on endosomes. However, molecular details on the role of Rab5 in Mon1-Ccz1 activation are unclear. Here, we identify key features in Mon1 involved in GEF regulation. We show that the intrinsically disordered N-terminal domain of Mon1 autoinhibits Rab5-dependent GEF activity on membranes...
July 25, 2023: Proceedings of the National Academy of Sciences of the United States of America
https://read.qxmd.com/read/37436410/-exosomes-in-the-life-cycle-of-viruses-and-the-pathogenesis-of-viral-infections
#33
JOURNAL ARTICLE
A A Kushch, A V Ivanov
Exosomes are extracellular vesicles of endosomal origin, with a bilayer membrane, 30160 nm in diameter. Exosomes are released from cells of different origins and are detected in various body fluids. They contain nucleic acids, proteins, lipids, metabolites and can transfer the contents to recipient cells. Exosome biogenesis involves cellular proteins of the Rab GTPase family and the ESCRT system, which regulate budding, vesicle transport, molecule sorting, membrane fusion, formation of multivesicular bodies and exosome secretion...
July 6, 2023: Voprosy Virusologii
https://read.qxmd.com/read/37423034/receptor-mediated-internalization-promotes-increased-endosome-size-and-number-in-a-rab4-and-rab5-dependent-manner
#34
JOURNAL ARTICLE
Naava Naslavsky, Steve Caplan
Despite their significance in receptor-mediated internalization and continued signal transduction in cells, early/sorting endosomes (EE/SE) remain incompletely characterized, with many outstanding questions that surround the dynamics of their size and number. While several studies have reported increases in EE/SE size and number resulting from endocytic events, few studies have addressed such dynamics in a methodological and quantitative manner. Herein we apply quantitative fluorescence microscopy to measure the size and number of EE/SE upon internalization of two different ligands: transferrin and epidermal growth factor...
July 6, 2023: European Journal of Cell Biology
https://read.qxmd.com/read/37365473/biochemically-reconstituted-fusion-of-phagosomes-with-endosomes-and-lysosomes
#35
JOURNAL ARTICLE
Andreas Jeschke, Albert Haas
Professional phagocytic cells, such as macrophages, ingest large particles into a specialized endocytic compartment, the phagosome, which eventually turns into a phagolysosome and degrades its contents. This phagosome "maturation" is governed by successive fusion of the phagosome with early sorting endosomes, late endosomes, and lysosomes. Further changes occur by fission of vesicles from the maturing phagosome and by on-and-off cycling of cytosolic proteins. We present here a detailed protocol which allows to reconstitute in a cell-free system the fusion events between phagosomes and the different endocytic compartments...
2023: Methods in Molecular Biology
https://read.qxmd.com/read/37351379/erratum-autophagy-and-neurodegeneration-unraveling-the-role-of-c9orf72-in-the-regulation-of-autophagy-and-its-relationship-to-als-ftd-pathology
#36
(no author information available yet)
[This corrects the article DOI: 10.3389/fncel.2023.1086895.].
2023: Frontiers in Cellular Neuroscience
https://read.qxmd.com/read/37340959/crucial-roles-of-rab22a-in-endosomal-cargo-recycling
#37
REVIEW
Lingjie Kong, Shenghao Huang, Yuxuan Bao, Yingtong Chen, Chunyan Hua, Sheng Gao
Endosomal cargo recycling lies at the heart of subcellular trafficking processes under the management of several Ras-related GTP-binding proteins (Rabs) which are coordinated by their upstream regulators and require their downstream effectors to display their functions. In this regard, several Rabs have been well-reviewed except Rab22a. Rab22a is a crucial regulator of vesicle trafficking, early endosome and recycling endosome formation. Notably, recent studies demonstrated the immunological roles of Rab22a, which are closely associated with cancers, infection and autoimmune disorders...
June 21, 2023: Traffic
https://read.qxmd.com/read/37315786/-disruption-of-golgi-markers-by-two-rilp-directed-shrnas-in-neurons-a-new-role-for-rilp-or-a-neuron-specific-off-target-phenotype
#38
JOURNAL ARTICLE
Chan Choo Yap, Laura Digilio, Lloyd McMahon, Bettina Winckler
In neurons, degradation of dendritic cargos requires RAB7 and dynein-mediated retrograde transport to somatic lysosomes. To test if the dynein adaptor RILP (RAB-interacting lysosomal protein) mediated the recruitment of dynein to late endosomes for retrograde transport in dendrites, we obtained several knockdown reagents previously validated in non-neuronal cells. Striking endosomal phenotypes elicited by one shRILP plasmid were not reproduced by another one. Furthermore, we discovered a profound depletion of Golgi/TGN markers for both shRILP plasmids...
June 12, 2023: Journal of Biological Chemistry
https://read.qxmd.com/read/37295229/emodin-and-rhapontigenin-inhibit-the-replication-of-african-swine-fever-virus-by-interfering-with-virus-entry
#39
JOURNAL ARTICLE
Yanchen Guo, Yang Chen, Qiumei Wang, Zhiyuan Wang, Lang Gong, Yankuo Sun, Zebu Song, Hao Chang, Guihong Zhang, Heng Wang
Africa swine fever (ASF) is a highly pathogenic contagion caused by African swine fever virus (ASFV), which not only affects the development of domestic pig industry, but also causes huge losses to the world agricultural economy. Vaccine development targeting ASFV remains elusive, which leads to severe difficulties in disease prevention and control. Emodin (EM) and rhapontigenin (RHAG), which are extracted from the dried rhizome of Polygonum knotweed, have various biological properties such as anti-neoplastic and anti-bacterial activities, but no studies have reported that they have anti-ASFV effects...
June 1, 2023: Veterinary Microbiology
https://read.qxmd.com/read/37267906/structural-basis-of-mrna-binding-by-the-human-ferry-rab5-effector-complex
#40
JOURNAL ARTICLE
Dennis Quentin, Jan S Schuhmacher, Björn U Klink, Jeni Lauer, Tanvir R Shaikh, Pim J Huis In 't Veld, Luisa M Welp, Henning Urlaub, Marino Zerial, Stefan Raunser
The pentameric FERRY Rab5 effector complex is a molecular link between mRNA and early endosomes in mRNA intracellular distribution. Here, we determine the cryo-EM structure of human FERRY. It reveals a unique clamp-like architecture that bears no resemblance to any known structure of Rab effectors. A combination of functional and mutational studies reveals that while the Fy-2 C-terminal coiled-coil acts as binding region for Fy-1/3 and Rab5, both coiled-coils and Fy-5 concur to bind mRNA. Mutations causing truncations of Fy-2 in patients with neurological disorders impair Rab5 binding or FERRY complex assembly...
June 1, 2023: Molecular Cell
keyword
keyword
169104
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.