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y-box binding protein 1 intestine

W Lee, O K Kwon, M-S Han, Y-M Lee, S-W Kim, K-M Kim, T Lee, S Lee, J-S Bae
Sepsis is a life-threatening condition that arises when the body's response to infection causes systemic inflammation. High-mobility group box 1 (HMGB1), as a late mediator of sepsis, enhances hyperpermeability, and it is therefore a therapeutic target. Despite extensive research into the underlying mechanisms of sepsis, the target molecules controlling vascular leakage remain largely unknown. Moesin is a cytoskeletal protein involved in cytoskeletal changes and paracellular gap formation. The objectives of this study were to determine the roles of moesin in HMGB1-mediated vascular hyperpermeability and inflammatory responses and to investigate the mechanisms of action underlying these responses...
August 2015: Thrombosis and Haemostasis
TianTian Guo, YingNan Yu, George Wai-Cheong Yip, Gyeong Hun Baeg, Aye Aye Thike, Tony Kiat-Hon Lim, Puay Hoon Tan, Ken Matsumoto, Boon Huat Bay
AIMS: Y-box binding protein-1 (YB-1) is known to modulate gene transcription and protein translation, as well as cellular response to drug treatment. The aim of this study is to correlate YB-1 protein expression levels with clinicopathological parameters in intestinal-type gastric cancer tissue samples (as categorized by the Lauren classification) and substantiate the findings with in vitro experimentation. METHODS AND RESULTS: Paraffin-embedded samples from 167 patients with intestinal-type gastric cancer were used for the construction of tissue microarrays (TMAs)...
March 2015: Histopathology
Sílvia R C Dias, Mariana Boroni, Elizângela A Rocha, Thomaz L Dias, Daniela de Laet Souza, Fabrício M S Oliveira, Mainá Bitar, Andrea M Macedo, Carlos R Machado, Marcelo V Caliari, Glória R Franco
Schistosomiasis is a neglected tropical disease, and after malaria, is the second most important tropical disease in public health. A vaccine that reduces parasitemia is desirable to achieve mass treatment with a low cost. Although potential antigens have been identified and tested in clinical trials, no effective vaccine against schistosomiasis is available. Y-box-binding proteins (YBPs) regulate gene expression and participate in a variety of cellular processes, including transcriptional and translational regulation, DNA repair, cellular proliferation, drug resistance, and stress responses...
2014: Frontiers in Genetics
Sandra Kirsch Heibel, Giselle Yvette Lopez, Maria Panglao, Sonal Sodha, Leonardo Mariño-Ramírez, Mendel Tuchman, Ljubica Caldovic
The urea cycle converts toxic ammonia to urea within the liver of mammals. At least 6 enzymes are required for ureagenesis, which correlates with dietary protein intake. The transcription of urea cycle genes is, at least in part, regulated by glucocorticoid and glucagon hormone signaling pathways. N-acetylglutamate synthase (NAGS) produces a unique cofactor, N-acetylglutamate (NAG), that is essential for the catalytic function of the first and rate-limiting enzyme of ureagenesis, carbamyl phosphate synthetase 1 (CPS1)...
2012: PloS One
Ying Wu, Sohsuke Yamada, Hiroto Izumi, Zhi Li, Shohei Shimajiri, Ke-Yong Wang, Yun-Peng Liu, Kimitoshi Kohno, Yasuyuki Sasaguri
BACKGROUND: The most significant cause of gastric cancer (GC) death is metastasis, although the underlying mechanisms remain obscure. Y-box binding protein-1 (YB-1) is associated with tumor aggressiveness and poor prognosis in various cancers. In this study we investigated the relationship between YB-1 expression and the clinicopathologic features and metastasis-associated epithelial-mesenchymal transition (EMT) phenotype in advanced GC patients. PATIENTS AND METHODS: Immunohistochemistry (IHC) was used to analyze YB-1, E-cadherin, and vimentin expression in 98 advanced GC cases...
June 1, 2012: Journal of Surgical Oncology
Y Gosmain, C Cheyssac, M Heddad Masson, C Dibner, J Philippe
The glucagon gene is expressed in α-cells of the pancreas, L cells of the intestine and the hypothalamus. The determinants of the α-cell-specific expression of the glucagon gene are not fully characterized, although Arx, Pax6 and Foxa2 are critical for α-cell differentiation and glucagon gene expression; in addition, the absence of the β-cell-specific transcription factors Pdx1, Pax4 and Nkx6.1 may allow for the glucagon gene to be expressed. Pax6, along with cMaf and MafB, binds to the DNA control element G(1) which confers α-cell specificity to the promoter and to G(3) and potently activates glucagon gene transcription...
October 2011: Diabetes, Obesity & Metabolism
Junhong Zhao, Hongchuan Jin, Kin Fai Cheung, Joanna H M Tong, Sui Zhang, Minnie Y Y Go, Linwei Tian, Wei Kang, Patrick P S Leung, Zhirong Zeng, Xiaoxing Li, Ka Fai To, Joseph J Y Sung, Jun Yu
BACKGROUND: The role of Epstein-Barr virus (EBV) infection in gastric carcinogenesis remains largely unknown. The authors studied the effects of zinc finger E-box binding factor 1 (ZEB1) on latent-lytic switch of EBV infection in gastric cancer and explored the importance of EBV in gastric carcinogenesis. METHODS: Loss or gain of ZEB1 function was obtained by ZEB1 small-interfering RNA (siRNA) knock-down or forced ZEB1 re-expression. Cell growth was evaluated by cell viability and colony formation assays, and the cell cycle was assessed by flow cytometry...
February 15, 2012: Cancer
Xiaoqiang Xiao, Qiwei Qin, Xinhua Chen
Toll-like receptors (TLRs) are a family of innate immune receptors that recognize molecular patterns associated with microbial pathogens (PAMP), and induce antimicrobial immune responses. Here we report the molecular cloning and characterization of a TLR22 homologue from the large yellow croaker (LycTLR22), an economically important marine fish in China. The complete cDNA of LycTLR22 is 4607 nucleotides (nt) long, encoding a protein of 963 amino acids (aa). The deduced LycTLR22 has a typical TLR domain architecture including 18 leucine-rich repeats (LRRs) (residues 69-638) and one C-terminal LRR domain (residues 698-749) at the extracellular region, and a TIR domain (residues 805-948) in the cytoplasmic region...
January 2011: Fish & Shellfish Immunology
Michael Buchert, Marina Papin, Caroline Bonnans, Charbel Darido, Warren S Raye, Véronique Garambois, André Pélegrin, Jean-François Bourgaux, Julie Pannequin, Dominique Joubert, Frédéric Hollande
Symplekin is a ubiquitously expressed protein involved in cytoplasmic RNA polyadenylation and transcriptional regulation and is localized at tight junctions (TJs) in epithelial cells. Nuclear symplekin cooperates with the Y-box transcription factor zonula occludens 1-associated nucleic acid-binding protein (ZONAB) to increase the transcription of cell cycle-related genes and also inhibits differentiation of intestinal cells. We detected high levels of nuclear symplekin in 8 of 12 human colorectal cancer (CRC) samples...
February 9, 2010: Proceedings of the National Academy of Sciences of the United States of America
Michael Buchert, Charbel Darido, Ebba Lagerqvist, Anna Sedello, Chantal Cazevieille, Frank Buchholz, Jean-Francois Bourgaux, Julie Pannequin, Dominique Joubert, Frédéric Hollande
BACKGROUND & AIMS: Symplekin is a ubiquitously expressed protein involved in RNA polyadenylation and transcriptional regulation that localizes at tight junctions in epithelial cells. The association between symplekin and the Y-box transcription factor ZONAB activates proliferation in intestinal and kidney cells. We analyzed symplekin expression in human colonic crypts and investigated its function in differentiation. METHODS: Expression of differentiation markers and transcription factors was assessed in HT29-Cl...
July 2009: Gastroenterology
Y Yan, H Nguyen, G Dalmasso, S V Sitaraman, D Merlin
Intestinal epithelial cells respond to inflammatory extracellular stimuli by activating mitogen activated protein kinase (MAPK) signaling, which mediates numerous pathophysiological effects, including intestinal inflammation. Here, we show that a novel isoform of SPS1-related proline alanine-rich kinase (SPAK/STE20) is involved in this inflammatory signaling cascade. We cloned and characterized a SPAK isoform from inflamed colon tissue, and found that this SPAK isoform lacked the characteristic PAPA box and alphaF loop found in SPAK...
February 2007: Biochimica et Biophysica Acta
Emma Kavanagh, Michael Buchert, Anna Tsapara, Armelle Choquet, Maria S Balda, Frédéric Hollande, Karl Matter
Epithelial tight junctions participate in the regulation of gene expression by controlling the activity of transcription factors that can interact with junctional components. One such protein is the Y-box transcription factor ZONAB/DbpA that binds to ZO-1, a component of the junctional plaque. Symplekin, another nuclear protein that can associate with tight junctions, functions in the regulation of polyadenylation and thereby promotes gene expression. Here, we addressed the question of whether these two proteins interact and whether this is of functional relevance...
December 15, 2006: Journal of Cell Science
Yizeng Yang, Gösta Eggertsen, Mats Gåfvels, Ulla Andersson, Curt Einarsson, Ingemar Björkhem, John Y L Chiang
Sterol 12alpha-hydroxylase (CYP8B1) is an obligatory enzyme for the synthesis of cholic acid and regulation of liver bile acid synthesis and intestine cholesterol absorption. The present study evaluates the roles for sterol regulatory element binding proteins (SREBPs) in the regulation of the CYP8B1 gene. Cholesterol feeding of mice and rats decreased the activity of CYP8B1, contrary to the up-regulation of cholesterol 7alpha-hydroxylase (CYP7A1). Cholesterol feeding also reduced mRNA levels for SREBP-1 but not for SREBP-2 in rat livers...
August 6, 2004: Biochemical and Biophysical Research Communications
Juha Mykkänen, Minna Toivonen, Maaria Kleemola, Marja-Liisa Savontaus, Olli Simell, Pertti Aula, Kirsi Huoponen
The human SLC7A7 gene on chromosome 14q11.2 encodes the y+L amino acid transporter-1 (y+LAT-1) protein that transports, together with the 4F2hc cell surface antigen, cationic amino acids through the basolateral membrane of epithelial cells in the small intestine and kidney. The SLC7A7 gene comprises 11 exons, but the first two are not translated. Mutations in the coding region of the SLC7A7 gene cause a rare autosomal disorder, lysinuric protein intolerance (LPI). We have now investigated the expression levels and putative 5' promoter elements of the SLC7A7...
February 21, 2003: Biochemical and Biophysical Research Communications
Daisuke Mori, Naoko Okuro, Yoshiaki Fujii-Kuriyama, Kazuhiro Sogawa
Rat BTEB2 protein is a transcription factor with three zinc fingers that binds to GC box, and is expressed in the placenta, intestine, and testis. To understand mechanisms of gene expression of BTEB2, we have cloned the rat BTEB2 gene from a rat liver genomic library and determined the gene structure. The BTEB2 gene contained 4 exons. In the process of cloning of the BTEB2 gene, we cloned two pseudogenes for BTEB2, one of which was a processed gene. The upstream region of the bona fide gene was fused to a luciferase reporter gene, and the generated BTEB2-luciferase chimeric plasmid was transiently transfected into HeLa cells that expressed endogenous BTEB2 mRNA...
January 30, 2003: Gene
S W Rue, B W Kim, D Y Jun, Y H Kim
The nucleotide sequence of the ZF5128 gene, encoding a novel Kruppel type zinc finger protein, has been determined. The ZF5128 gene has a predicted 553-amino acid open reading frame, encoding a putative 61 kDa zinc finger protein. The N-terminus of the ZF5128 coding region has a well-conserved Kruppel-associated box (KRAB) domain that consists of KRAB box A and B, whereas the C-terminus contains a Kruppel type C2H2 zinc finger domain possessing nine C2H2 zinc finger motifs in tandem arrays with the highly conserved space region of the H/C-link...
December 30, 2001: Biochimica et Biophysica Acta
Y Schneider, B Duranton, F Gossé, R Schleiffer, N Seiler, F Raul
We studied the effect of oral administration of resveratrol, a natural constituent of grapes, on tumorigenesis in Min mice. Min mice are congenic mice genetically predisposed to develop intestinal tumors as a result of a mutation of the Apc gene. Resveratrol (0.01% in the drinking water containing 0.4% ethanol) was administered for seven weeks to Min mice starting at five weeks of age. The control group was fed the same diet and received water containing 0.4% ethanol. Resveratrol prevented the formation of colon tumors and reduced the formation of small intestinal tumors by 70%...
2001: Nutrition and Cancer
B L Song, W Qi, X Y Yang, C C Chang, J Q Zhu, T Y Chang, B L Li
Acyl-CoA:cholesterol acyltransferase (ACAT) plays important roles in cellular cholesterol homeostasis. Two ACAT genes exist in mammals. We report here the genomic organization of human ACAT-2 gene and analysis of its promoter activity in various cell lines. The human ACAT-2 gene spans over 18 kb and contains 15 exons. Three transcription start sites and one poly(A) site are identified by the 5'/3'-RACE. In addition, the human ACAT-2 gene is linked to the insulin-like growth factor binding protein 6 (IGFBP-6) gene in a head-to-tail manner with a small intergenic region of about 1...
March 30, 2001: Biochemical and Biophysical Research Communications
J Malakooti, R Y Dahdal, P K Dudeja, T J Layden, K Ramaswamy
The Na(+)/H(+) exchanger (NHE) 2 belongs to a family of plasma membrane transporters involved in intracellular pH and cell volume regulation. We recently reported cloning of human NHE2 (hNHE2) from a colonic cDNA library. Northern blot analysis has identified NHE2 mRNA only in small intestine, prostate, kidney, colon, and skeletal muscle. In this study, we describe the structure and 5'-regulatory region of the hNHE2 gene. The hNHE2 gene spans >90 kb and is organized in 12 exons intervened by 11 introns. All introns contain the conserved GT and AG dinucleotides at the donor and acceptor sites, respectively...
April 2001: American Journal of Physiology. Gastrointestinal and Liver Physiology
R H Erickson, R S Lai, C D Lotterman, Y S Kim
The 5' upstream region (-448/-443) of the human dipeptidyl peptidase IV gene promoter containing a consensus E-box (CACGTG) was shown to bind upstream stimulatory factor using nuclear extracts from mouse (3T3) fibroblasts and the human intestinal and hepatic epithelial cell lines Caco-2 and HepG2. Supershift analysis with specific antibodies to USF-1 and USF-2 indicates that USF-1 is the primary isoform binding to the E-box in nuclear extracts from these cells. Using cell culture, transient cotransfection of USF expression vectors with dipeptidyl peptidase IV promoter constructs revealed that both USF-1 and USF-2 caused an approximately tenfold increase in reporter gene expression in Caco-2 cells...
November 27, 2000: Gene
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