keyword
https://read.qxmd.com/read/38297086/tropomyosin-1-i-c-coordinates-kinesin-1-and-dynein-motors-during-oskar-mrna-transport
#21
JOURNAL ARTICLE
Simone Heber, Mark A McClintock, Bernd Simon, Eve Mehtab, Karine Lapouge, Janosch Hennig, Simon L Bullock, Anne Ephrussi
Dynein and kinesin motors mediate long-range intracellular transport, translocating towards microtubule minus and plus ends, respectively. Cargoes often undergo bidirectional transport by binding to both motors simultaneously. However, it is not known how motor activities are coordinated in such circumstances. In the Drosophila female germline, sequential activities of the dynein-dynactin-BicD-Egalitarian (DDBE) complex and of kinesin-1 deliver oskar messenger RNA from nurse cells to the oocyte, and within the oocyte to the posterior pole...
January 31, 2024: Nature Structural & Molecular Biology
https://read.qxmd.com/read/38293064/axonal-distribution-of-mitochondria-maintains-neuronal-autophagy-during-aging-via-eif2%C3%AE
#22
Kanako Shinno, Yuri Miura, Koichi M Iijima, Emiko Suzuki, Kanae Ando
UNLABELLED: Neuronal aging and neurodegenerative diseases are accompanied by proteostasis collapse, while cellular factors that trigger it are not identified. Impaired mitochondrial transport in the axon is another feature of aging and neurodegenerative diseases. Using Drosophila , we found that genetic depletion of axonal mitochondria causes dysregulation of translation and protein degradation. Axons with mitochondrial depletion showed abnormal protein accumulation, and autophagic defects...
January 20, 2024: bioRxiv
https://read.qxmd.com/read/38277121/transgenerational-effects-of-maternal-zinc-deficiency-on-zinc-transporters-in-drosophila-melanogaster
#23
JOURNAL ARTICLE
Kamaldeen Olalekan Sanusi, Murtala Bello Abubakar, Kasimu Ghandi Ibrahim, Mustapha Umar Imam
Maternal nutrition, including the availability of micronutrients such as zinc, influences the health of the offspring. Using Drosophila melanogaster, we studied the impact of zinc deficiency on development and reproduction, as well as the effects of maternal zinc status on the offspring's expression of zinc transporters across F1 to F3 generations. Zinc deficiency was induced by adding N,N,N',N'-Tetrakis (2-pyridylmethyl)-ethylenediamine (TPEN) to the diet on which the eggs representing the F0 generation flies were laid...
January 26, 2024: Biological Trace Element Research
https://read.qxmd.com/read/38271216/exploring-the-k-binding-site-and-its-coupling-to-transport-in-the-neurotransmitter-sodium-symporter-leut
#24
JOURNAL ARTICLE
Solveig G Schmidt, Andreas Nygaard, Joseph A Mindell, Claus J Loland
The neurotransmitter:sodium symporters (NSSs) are secondary active transporters that couple the reuptake of substrate to the symport of one or two sodium ions. One bound Na+ (Na1) contributes to the substrate binding, while the other Na+ (Na2) is thought to be involved in the conformational transition of the NSS. Two NSS members, the serotonin transporter (SERT) and the Drosophila dopamine transporter (dDAT), also couple substrate uptake to the antiport of K+ by a largely undefined mechanism. We have previously shown that the bacterial NSS homologue, LeuT, also binds K+ , and could therefore serve as a model protein for the exploration of K+ binding in NSS proteins...
January 25, 2024: ELife
https://read.qxmd.com/read/38264934/role-of-bicdr-in-bristle-shaft-construction-and-support-of-bicd-functions
#25
JOURNAL ARTICLE
Aleksandra Jejina, Yeniffer Ayala, Dirk Beuchle, Thomas Höhener, Ruth E Dörig, Paula Vazquez-Pianzola, Greco Hernández, Beat Suter
Cell polarization requires asymmetric localization of numerous mRNAs, proteins, and organelles. The movement of cargo towards the minus end of microtubules mostly depends on cytoplasmic dynein motors. In the dynein/dynactin/Bicaudal-D transport machinery, Bicaudal-D (BicD) links the cargo to the motor. Here we focus on the role of Drosophila BicD-related (BicDR) in the development of the long bristles. Together with BicD, it contributes to the organization and stability of the actin cytoskeleton in the not-yet chitinized bristle shaft...
January 24, 2024: Journal of Cell Science
https://read.qxmd.com/read/38263196/in-vivo-identification-of-drosophila-rhodopsin-interaction-partners-by-biotin-proximity-labeling
#26
JOURNAL ARTICLE
Nilofar Feizy, Sarah Franziska Leuchtenberg, Christine Steiner, Berit Würtz, Leo Fliegner, Armin Huber
Proteins exert their function through protein-protein interactions. In Drosophila, G protein-coupled receptors like rhodopsin (Rh1) interact with a G protein to activate visual signal transduction and with arrestins to terminate activation. Also, membrane proteins like Rh1 engage in protein-protein interactions during folding within the endoplasmic reticulum, during their vesicular transport and upon removal from the cell surface and degradation. Here, we expressed a Rh1-TurboID fusion protein (Rh1::TbID) in Drosophila photoreceptors to identify in vivo Rh1 interaction partners by biotin proximity labeling...
January 23, 2024: Scientific Reports
https://read.qxmd.com/read/38260657/starvation-resistant-cavefish-reveal-conserved-mechanisms-of-starvation-induced-hepatic-lipotoxicity
#27
Macarena Pozo-Morales, Ansa E Cobham, Cielo Centola, Mary Cathleen McKinney, Peiduo Liu, Camille Perazzolo, Anne Lefort, Frédérick Libert, Hua Bai, Nicolas Rohner, Sumeet Pal Singh
UNLABELLED: Starvation causes the accumulation of lipid droplets in the liver, a somewhat counterintuitive phenomenon that is nevertheless conserved from flies to humans. Much like fatty liver resulting from overfeeding, hepatic lipid accumulation (steatosis) during undernourishment can lead to lipotoxicity and atrophy of the liver. Here, we found that while surface populations of Astyanax mexicanus undergo this evolutionarily conserved response to starvation, the starvation-resistant cavefish larvae of the same species do not display an accumulation of lipid droplets upon starvation...
January 11, 2024: bioRxiv
https://read.qxmd.com/read/38251707/phosphate-starvation-signaling-increases-mitochondrial-membrane-potential-through-respiration-independent-mechanisms
#28
JOURNAL ARTICLE
Yeyun Ouyang, Mi-Young Jeong, Corey N Cunningham, Jordan A Berg, Ashish G Toshniwal, Casey E Hughes, Kristina Seiler, Jonathan G Van Vranken, Ahmad A Cluntun, Geanette Lam, Jacob M Winter, Emel Akdoǧan, Katja K Dove, Sara M Nowinski, Matthew West, Greg Odorizzi, Steven P Gygi, Cory D Dunn, Dennis R Winge, Jared Rutter
Mitochondrial membrane potential directly powers many critical functions of mitochondria, including ATP production, mitochondrial protein import, and metabolite transport. Its loss is a cardinal feature of aging and mitochondrial diseases, and cells closely monitor membrane potential as an indicator of mitochondrial health. Given its central importance, it is logical that cells would modulate mitochondrial membrane potential in response to demand and environmental cues, but there has been little exploration of this question...
January 22, 2024: ELife
https://read.qxmd.com/read/38234787/constitutive-and-conditional-epitope-tagging-of-endogenous-g-protein-coupled-receptors-in-drosophila
#29
Shivan L Bonanno, Piero Sanfilippo, Aditya Eamani, Maureen M Sampson, Kandagedon Binu, Kenneth Li, Giselle D Burns, Marylyn E Makar, S Lawrence Zipursky, David E Krantz
UNLABELLED: To visualize the cellular and subcellular localization of neuromodulatory G-protein coupled receptors (GPCRs) in Drosophila , we implement a molecular strategy recently used to add epitope tags to ionotropic receptors at their endogenous loci. Leveraging evolutionary conservation to identify sites more likely to permit insertion of a tag, we generated constitutive and conditional tagged alleles for Drosophila 5-HT1A, 5-HT2A, 5-HT2B, Octβ1R, Octβ2R, two isoforms of OAMB, and mGluR...
December 27, 2023: bioRxiv
https://read.qxmd.com/read/38233994/drosophila-clc-c-is-a-homolog-of-human-clc-5-and-a-new-model-for-dent-disease-type-1
#30
JOURNAL ARTICLE
Carmen J Reynolds, Christopher M Gillen, Richard Burke, Yula Tsering, Emi Loucks, Sebastian Judd-Mole, Julian A T Dow, Michael F Romero
BACKGROUND: Drosophila serve as exceptional alternative models for in vivo and ex vivo research and may provide an avenue for in-depth investigation for human ClC-5 and Dent disease type 1 (DD1). The Drosophila ClC-c (CG5284) has sequence homology with human ClC-5 and is hypothesized to encompass similar functional and phenotypical roles with ClC-5 and variants that cause DD1. METHODS: Ion transport function and activity of Drosophila ClC-c and homologous DD1 variants were assessed by voltage clamp electrophysiology...
January 18, 2024: Kidney360
https://read.qxmd.com/read/38229174/intracellular-delivery-of-parkin-ring0-based-fragments-corrects-parkin-induced-mitochondrial-dysfunction-through-interaction-with-slp-2
#31
JOURNAL ARTICLE
Alessandra Zanon, Marianna Guida, Alexandros A Lavdas, Corrado Corti, Maria Paulina Castelo Rueda, Alessandro Negro, Peter P Pramstaller, Francisco S Domingues, Andrew A Hicks, Irene Pichler
BACKGROUND: Loss-of-function mutations in the PRKN gene, encoding Parkin, are the most common cause of autosomal recessive Parkinson's disease (PD). We have previously identified mitoch ondrial Stomatin-like protein 2 (SLP-2), which functions in the assembly of respiratory chain proteins, as a Parkin-binding protein. Selective knockdown of either Parkin or SLP-2 led to reduced mitochondrial and neuronal function in neuronal cells and Drosophila, where a double knockdown led to a further worsening of Parkin-deficiency phenotypes...
January 16, 2024: Journal of Translational Medicine
https://read.qxmd.com/read/38214012/atg8-family-proteins-lir-aim-motifs-and-other-interaction-modes
#32
JOURNAL ARTICLE
Vladimir V Rogov, Ioannis P Nezis, Panagiotis Tsapras, Hong Zhang, Yasin Dagdas, Nobuo N Noda, Hitoshi Nakatogawa, Martina Wirth, Stephane Mouilleron, David G McEwan, Christian Behrends, Vojo Deretic, Zvulun Elazar, Sharon A Tooze, Ivan Dikic, Trond Lamark, Terje Johansen
The Atg8 family of ubiquitin-like proteins play pivotal roles in autophagy and other processes involving vesicle fusion and transport where the lysosome/vacuole is the end station. Nuclear roles of Atg8 proteins are also emerging. Here, we review the structural and functional features of Atg8 family proteins and their protein-protein interaction modes in model organisms such as yeast, Arabidopsis, C. elegans and Drosophila to humans. Although varying in number of homologs, from one in yeast to seven in humans, and more than ten in some plants, there is a strong evolutionary conservation of structural features and interaction modes...
December 31, 2023: Autophagy Rep
https://read.qxmd.com/read/38213621/golgi-to-er-retrograde-transport-prevents-premature-differentiation-of-drosophila-type-ii-neuroblasts-via-notch-signal-sending-daughter-cells
#33
JOURNAL ARTICLE
Huanhuan Zhang, Menglong Rui, Zhixin Ma, Sifan Gong, Shuliu Zhang, Qingxia Zhou, Congfeng Gan, Wenting Gong, Su Wang
Stem cells are heterogeneous to generate diverse differentiated cell types required for organogenesis; however, the underlying mechanisms that differently maintain these heterogeneous stem cells are not well understood. In this study, we identify that Golgi-to-endoplasmic reticulum (ER) retrograde transport specifically maintains type II neuroblasts (NBs) through the Notch signaling. We reveal that intermediate neural progenitors (INPs), immediate daughter cells of type II NBs, provide Delta and function as the NB niche...
January 19, 2024: IScience
https://read.qxmd.com/read/38167886/monitoring-lipid-alterations-in-drosophila-heads-in-an-amyotrophic-lateral-sclerosis-model-with-time-of-flight-secondary-ion-mass-spectrometry
#34
JOURNAL ARTICLE
Minh Uyen Thi Le, Jeong Hyang Park, Jin Gyeong Son, Hyun Kyung Shon, Sunho Joh, Chang Geon Chung, Jae Ho Cho, Alexander Pirkl, Sung Bae Lee, Tae Geol Lee
Lipid alterations in the brain are well-documented in disease and aging, but our understanding of their pathogenic implications remains incomplete. Recent technological advances in assessing lipid profiles have enabled us to intricately examine the spatiotemporal variations in lipid compositions within the complex brain characterized by diverse cell types and intricate neural networks. In this study, we coupled time-of-flight secondary ion mass spectrometry (ToF-SIMS) to an amyotrophic lateral sclerosis (ALS) Drosophila model, for the first time, to elucidate changes in the lipid landscape and investigate their potential role in the disease process, serving as a methodological and analytical complement to our prior approach that utilized matrix-assisted laser desorption/ionization mass spectrometry...
January 3, 2024: Analyst
https://read.qxmd.com/read/38167424/glia-as-functional-barriers-and-signaling-intermediaries
#35
REVIEW
Vilaiwan M Fernandes, Vanessa Auld, Christian Klämbt
Glia play a crucial role in providing metabolic support to neurons across different species. To do so, glial cells isolate distinct neuronal compartments from systemic signals and selectively transport specific metabolites and ions to support neuronal development and facilitate neuronal function. Because of their function as barriers, glial cells occupy privileged positions within the nervous system and have also evolved to serve as signaling intermediaries in various contexts. The fruit fly, Drosophila melanogaster , has significantly contributed to our understanding of glial barrier development and function...
January 2, 2024: Cold Spring Harbor Perspectives in Biology
https://read.qxmd.com/read/38161240/the-role-of-secondary-structures-in-the-functioning-of-3-untranslated-regions-of-mrna-a-review-of-functions-of-3-utrs-secondary-structures-and-hypothetical-involvement-of-secondary-structures-in-cytoplasmic-polyadenylation-in-drosophila
#36
JOURNAL ARTICLE
Mariya Zhukova, Paul Schedl, Yulii V Shidlovskii
3' untranslated regions (3' UTRs) of mRNAs have many functions, including mRNA processing and transport, translational regulation, and mRNA degradation and stability. These different functions require cis-elements in 3' UTRs that can be either sequence motifs or RNA structures. Here we review the role of secondary structures in the functioning of 3' UTRs and discuss some of the trans-acting factors that interact with these secondary structures in eukaryotic organisms. We propose potential participation of 3'-UTR secondary structures in cytoplasmic polyadenylation in the model organism Drosophila melanogaster...
December 31, 2023: BioEssays: News and Reviews in Molecular, Cellular and Developmental Biology
https://read.qxmd.com/read/38139239/-drosophila-melanogaster-transcriptome-response-to-different-wolbachia-strains
#37
JOURNAL ARTICLE
Nataly E Gruntenko, Maksim A Deryuzhenko, Olga V Andreenkova, Olga D Shishkina, Margarita A Bobrovskikh, Natalja V Shatskaya, Gennady V Vasiliev
Wolbachia is a maternally inherited, intercellular bacterial symbiont of insects and some other invertebrates. Here, we investigated the effect of two different Wolbachia strains, differing in a large chromosomal inversion, on the differential expression of genes in D. melanogaster females. We revealed significant changes in the transcriptome of the infected flies compared to the uninfected ones, as well as in the transcriptome of flies infected with the Wolbachia strain, wMelPlus, compared to flies infected with the wMelCS112 strain...
December 12, 2023: International Journal of Molecular Sciences
https://read.qxmd.com/read/38118175/stress-pathways-induced-by-volatile-anesthetics-and-failure-of-preconditioning-in-a-mitochondrial-complex-i-mutant
#38
JOURNAL ARTICLE
Zachariah P G Olufs, David A Wassarman, Misha Perouansky
BACKGROUND: Carriers of mutations in the mitochondrial electron transport chain are at increased risk of anesthetic-induced neurotoxicity. To investigate the neurotoxicity mechanism and to test preconditioning as a protective strategy, this study used a Drosophila melanogaster model of Leigh syndrome. Model flies carried a mutation in ND23 (ND2360114) that encodes a mitochondrial electron transport chain complex I subunit. This study investigated why ND2360114 mutants become susceptible to lethal, oxygen-modulated neurotoxicity within 24 h of exposure to isoflurane but not sevoflurane...
March 1, 2024: Anesthesiology
https://read.qxmd.com/read/38106047/activity-dependent-capture-of-neuropeptide-vesicles-prepares-clock-neuron-synapses-for-daily-release
#39
Markus K Klose, Junghun Kim, Edwin S Levitan
Drosophila brain sLNv clock neurons release the neuropeptide PDF to control circadian rhythms. Strikingly, PDF content in sLNv terminals is rhythmic with a peak in the morning. Peak content drops because of activity-dependent release from dense-core vesicles (DCVs), but the mechanism for the daily increase in presynaptic PDF in the hours prior to release is unknown. Although transport from the soma was proposed to drive the daily increase in presynaptic PDF, live imaging in sLNv neurons shows that anterograde axonal DCV transport is constant throughout the day...
December 4, 2023: bioRxiv
https://read.qxmd.com/read/38103869/microtubule-dependent-apical-polarization-of-basement-membrane-matrix-mrnas-in-mouse-epithelial-cells
#40
JOURNAL ARTICLE
Shaohe Wang, Kazue Matsumoto, Madison M Mehlferber, Guofeng Zhang, Maria A Aronova, Kenneth M Yamada
The basement membrane (BM) demarcating epithelial tissues undergoes rapid expansion to accommodate tissue growth and morphogenesis during embryonic development. To facilitate the secretion of bulky BM proteins, their mRNAs are polarized basally in the follicle epithelial cells of the Drosophila egg chamber to position their sites of production close to their deposition. In contrast, we observed the apical rather than basal polarization of all major BM mRNAs in the outer epithelial cells adjacent to the BM of mouse embryonic salivary glands using single-molecule RNA fluorescence in situ hybridization (smFISH)...
December 14, 2023: Cells & development
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