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Hk-2 cell

Zhang Yifan, Xu Luwei, Liang Kai, Zhou Liuhua, Ge Yuzheng, Jia Ruipeng
The aim of this study was to investigate the role of oxidative stress in cystine crystal formation and whether salvianolic acid B, a natural antioxidant, could prevent cystine-mediated oxidative injury in vivo and in vitro. The levels of oxidative stress and antioxidase activity in cystine stone patients were assessed. Then, the oxidative stress exerted by cystine on human kidney-2 (HK-2) cell viability and biochemical parameters including antioxidase activity and antioxidant protein expression were evaluated, and the protective action of salvianolic acid B was also examined...
February 18, 2019: Urolithiasis
Zhanjun Ma, Xue Cui, Li Lu, Guohu Chen, Yang Yang, Yan Hu, Yubao Lu, Zhangqi Cao, Yan Wang, Xuexi Wang
BACKGROUND: Exosomes are nanoscale membrane vesicles secreted by both normal and cancer cells, and cancer cell-derived exosomes play an important role in the cross-talk between cancer cells and other cellular components in the tumor microenvironment. Mesenchymal stem cells (MSCs) have tropism for tumors and have been used as tumor-tropic vectors for tumor therapy; however, the safety of such therapeutic use of MSCs is unknown. In this study, we investigated the role of glioma cell-derived exosomes in the tumor-like phenotype transformation of human bone marrow mesenchymal stem cells (hBMSCs) and explored the underlying molecular mechanisms...
February 15, 2019: Stem Cell Research & Therapy
Jie Wu, Dan-Dan Li, Jia-Yao Li, Yi-Cong Yin, Peng-Chang Li, Ling Qiu, Li-Meng Chen
Cisplatin is a widely used chemotherapeutic drug that often causes acute kidney injury (AKI) in cancer patients. The contribution of miRNAs to the cisplatin-induced renal tubular epithelial cell injury remains largely unknown. Here we performed an integrative network analysis of miRNA and mRNA expression profiles to shed light into the underlying mechanism of cisplatin-induced renal tubular epithelial cell injury. Microarray analysis identified 47 differentially expressed miRNAs, among them 26 were upregulated and 21 were downregulated...
February 12, 2019: European Journal of Pharmacology
Yebei Li, Daijin Ren, Gaosi Xu
Several recent reporters have indicated the potential role of long noncoding RNA metastasis associated lung adenocarcinoma transcript 1 (MALAT1) in diabetic nephropathy (DN). However, these studies mainly focus on renal tubular epithelial cells HK-2, the role of MALAT1 in human renal glomerular endothelial cells (HRGECs) remains unclear. Hence, this study aimed to explore the role of MALAT1 in high glucose (HG)-induced HRGECs injury and the underlying epigenetic mechanism. Increased MALAT1 and decreased klotho expression were observed in both renal tissues from DN patients and HG-exposed HRGECs...
February 14, 2019: IUBMB Life
Yaeni Kim, Seun Deuk Hwang, Ji Hee Lim, Min Young Kim, Eun Nim Kim, Bum Soon Choi, Yong-Soo Kim, Hye Won Kim, Cheol Whee Park
Lymphangiogenesis occurs in response to renal injury and is correlated with interstitial fibrosis. Diabetes- and high-fat diet (HFD)-induced intrarenal lipotoxicity and their relationships with lymphangiogenesis are not established. We used PPARα agonist, fenofibrate, to unravel the linkage between lipotoxicity and lymphangiogenesis. Eight-week-old male C57BLKS/J db/db mice and HFD Spontaneously hypertensive rats (SHRs) were fed fenofibrate for 12 weeks. HK-2 and RAW264.7 cells were used to investigate their lymphangiogenic capacity in relation to lipotoxicity...
February 13, 2019: Scientific Reports
Z Lan, K Chai, Y Jiang, X Liu
The aim of this study was to identify biomarkers of zoledronate-induced nephrotoxicity and to further characterize the mechanisms underlying this process by analyzing urinary metabolites. Twenty-four rats were randomly divided into four groups containing four (two control groups) or eight rats (two zoledronate groups) per group. The rats were injected intravenously with saline or zoledronate (3 mg/kg) singly (single, 3 weeks) or repeatedly eight times (3 weeks/time, 24 weeks). Serum blood urea nitrogen, serum creatinine, creatinine clearance, and kidney injury observed by hematoxylin and eosin and immunohistochemical staining were changed only in the repeated zoledronate group (3 mg/kg, 3 weeks/time, 24 weeks)...
February 11, 2019: Human & Experimental Toxicology
Xiong Yang, Tong Yang, Jie Li, Rui Yang, Shiyong Qi, Yang Zhao, Liang Li, Jingjin Li, Xuening Zhang, Kuo Yang, Yong Xu, Chunyu Liu
Treatment targeting osteopontin (OPN) and monocyte chemoattractant protein 1 (MCP‑1) has been recognized as a novel approach in renal crystal formation. The present study was designed to investigate the suppressive effects of metformin on nephrolithiasis formation and its potential mechanism. The cytotoxicity of metformin on MDCK and HK‑2 cells was determined using a Cell Counting Kit‑8 assay in vitro. Subsequently, the mRNA transcription and protein expression levels of MCP‑1 and OPN were detected by reverse transcription‑quantitative‑polymerase chain reaction analysis, western blot analysis and ELISA...
January 30, 2019: International Journal of Molecular Medicine
N Singh, M Siddarth, R Ghosh, A K Tripathi, B D Banerjee
This study investigated the effect of heptachlor-induced oxidative stress (OS) on transforming growth factor (TGF)-β1-mediated epithelial to mesenchymal transition (EMT) in human renal proximal tubular epithelial (HK-2) cells. Following treatment of HK-2 cells with an increasing concentration of heptachlor (0.01-10 µM) for 24 h, the intracellular reactive oxygen species and malondialdehyde level increased, whereas the glutathione-s-hydroxylase (GSH) level declined significantly in a dose-dependent manner...
February 5, 2019: Human & Experimental Toxicology
Mi Jeong Kim, Chul-Hee Lee, Youngeun Lee, Hyewon Youn, Keon Wook Kang, JoonHo Kwon, Abass Alavi, Sean Carlin, Gi Jeong Cheon, June-Key Chung
PURPOSE: 2-Deoxy-2-[18 F]fluoro-D-glucose ([18 F]FDG) accumulation in inflammatory lesions can confound the diagnosis of cancer. In this study, we investigated [18 F]FDG accumulation and efflux in relation to the genes and proteins involved in glucose metabolism in murine inflammation and cancer models. PROCEDURES: [18 F]FDG accumulation and [18 F]FDG efflux were measured in cancer cells (breast cancer, glioma, thyroid cancer, and hepatoma cells) and RAW 264.7 cells (macrophages) activated with lipopolysaccharide (LPS)...
February 4, 2019: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Jie Li, Yu-Jun Tan, Ming-Zhi Wang, Ying Sun, Guang-Yan Li, Qi-Long Wang, Jing-Chun Yao, Jiang Yue, Zhong Liu, Gui-Min Zhang, Yu-Shan Ren
BACKGROUND AND PURPOSE: Acute kidney injury (AKI) is a rapid renal dysfunctional disease, for which no effective drugs or therapies are available to improve prognosis. Loganetin is a natural product with unknown bioactivities. Here we identified a new protective effect and mechanism of Loganetin in rhabdomyolysis-induced AKI mice model. EXPERIMENTAL APPROACH: AKI was induced using glycerol by intramuscular injection in mice models. Thirty minutes, 24 h and 48 h after injection of glycerol, the mice received 2 and 18 mg/kg of Loganetin i...
January 31, 2019: British Journal of Pharmacology
Fengjiao Zhang, Frances M Munoz, Lanlan Sun, Shuya Zhang, Serrine S Lau, Terrence J Monks
2,3,5-tris(Glutathion-S-yl)hydroquinone (TGHQ), a potent nephrotoxic and nephroncarcinogenic metabolite of benzene and hydroquinone, retains the ability to redox cycle and create oxidative stress. We have previously detected that TGHQ induces ROS-dependent necrotic or apoptotic cell death in renal epithelial HK-2 and human leukemic HL-60 cells respectively. Herein, we sought to determine the nature of the Nrf2 regulation in HK-2 and HL-60 cells undergoing TGHQ-mediated ROS-dependent cell death, due to the key role of Nrf2 in oxidative stress...
January 28, 2019: Chemico-biological Interactions
Giovanna Priante, Monica Ceol, Lisa Gianesello, Claudio Furlan, Dorella Del Prete, Franca Anglani
Nephrocalcinosis is a clinicopathological entity characterized by microscopic calcium crystals in the renal parenchyma, within the tubular lumen or in the interstitium. Crystal binding to tubular cells may be the cause underlying nephrocalcinosis and nephrolithiasis. Pathological circumstances, such as acute cortical necrosis, may induce healthy cells to acquire a crystal-binding phenotype. The present study aimed to investigate whether human renal proximal tubular cells (HK-2 cells) can form calcium phosphate deposits under osteogenic conditions, and whether apoptosis and/or osteogenic-like processes are involved in cell calcification...
2019: Cell Death Discovery
Yang Zhang, Ling Wang, Lei Meng, Guangke Cao, Yu Wu
Sirtuin 6 (SIRT6) has the function of regulating autophagy. The aim of this study was to investigate the mechanism through which SIRT6 relieved acute kidney injury (AKI) caused by sepsis. The AKI model was established with lipopolysaccharides (LPS) using mice. Hematoxylin-eosin (HE) staining and streptavidin-perosidase (SP) staining was used to observe kidney tissue and test SIRT6 and LC3B proteins in kidney. Enzyme-linked immunosorbent assay (ELISA) was performed to detected the tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) concentrations...
January 30, 2019: Cell Cycle
Mei-Chen Lo, Ming-Hong Chen, Yu-Ting Hsueh, Yung-Ting Kuo, Horng-Mo Lee
Nε -(carboxymethyl) lysine (CML) plays causal roles in diabetic complications. In the present study, we investigated whether CML-induced HIF-1α accumulation and epithelial-mesenchymal transition (EMT) in HK-2 renal proximal tubular epithelial cells. Treatment with CML-BSA increased reactive oxygen species (ROS) production reduced the mitochondrial membrane potential and induced mitochondrial fragmentation. Pre-treatment of cells with antioxidant, α-lipoic acid, normalised the ROS production and restored the mitochondrial membrane potential...
December 2018: Free Radical Research
Liping Wang, Ning Liu, Xiaoyan Xue, Shujun Zhou
BACKGROUND Acute kidney injury (AKI) involves the renal tubular epithelium. The enhancer of zeste homolog 1 (EZH1) gene has a role in cell development and differentiation. This study aimed to investigate the effect of overexpression of the EZH1 gene on aristolochic acid-induced injury in HK-2 human kidney proximal tubule epithelial cells in vitro. MATERIAL AND METHODS The HK-2 cells were cultured and treated with aristolochic acid and the effects of aristolochic acid-injury were evaluated using a cell counting kit-8 (CCK-8) assay...
January 28, 2019: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Xiao Jiang, Xiao-Hui Liao, Li-Li Huang, Hang Sun, Qi Liu, Ling Zhang
Ischemia/reperfusion is a major cause of acute kidney injury and can induce apoptosis in renal epithelial tubule (HK-2) cells. Accumulating evidence indicates that endoplasmic reticulum (ER) stress is a major contributor to apoptosis in acute kidney injury. We previously reported that augmenter of liver regeneration (ALR) functions as an anti-apoptotic factor in H2 O2 -treated HK-2 cells although the precise mechanism underlying this action remains unclear. In the present study, we investigate the role of ALR in H2 O2 -induced ER stress-mediated apoptosis...
January 24, 2019: Apoptosis: An International Journal on Programmed Cell Death
Li Yuan, Ping-Ping Liao, Hai-Cheng Song, Jia-Hui Zhou, Hai-Chen Chu, Lin Lyu
AIMS: Hyperbilirubinemia is associated with postoperative acute kidney injury in patients undergoing cardiac surgeries. A high concentration of bilirubin could induce oxidative stress and cell apoptosis. The aim of this study was to investigate whether hyperbilirubinemia aggravated the renal tubule cells injury and the pro-apoptotic potential of bilirubin on renal ischemia reperfusion injury (RIRI). METHODS: The human proximal tubular epithelial cell line HK-2 cells were challenged with a gradient concentration of bilirubin for 24 h...
January 23, 2019: Nephron
Martina Daga, Stefania Pizzimenti, Chiara Dianzani, Marie Angele Cucci, Roberta Cavalli, Margherita Grattarola, Benedetta Ferrara, Valentina Scariot, Francesco Trotta, Giuseppina Barrera
BACKGROUND: Ailanthone (Aila) is a natural active compound isolated from the Ailanthus altissima, which has been shown to possess an "in vitro" growth-inhibitory effect against several cancer cell lines. Advanced bladder cancer is a common disease characterized by a frequent onset of resistance to cisplatin-based therapy. The cisplatin (CDDP) resistance is accompanied by an increase in Nrf2 protein expression which contributes to conferring resistance. Recently, we demonstrated a cross-talk between Nrf2 and YAP...
October 29, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Lai Wang, Yang Liu, Ting-Li Zhao, Zheng-Zheng Li, Jin-Yong He, Ben-Jia Zhang, Hong-Zhi Du, Jing-Wei Jiang, Sheng-Tao Yuan, Li Sun
BACKGROUND: Topotecan (TPT) is a Topo I inhibitor and shows obvious anti-cancer effects on gastric cancer. Cancer cells reprogram their metabolic pathways to increase nutrients uptake, which has already been a hallmark of cancer. But the effect of TPT on metabolism in gastric cancer remains unknown. PURPOSE: To investigate the effect of TPT on metabolism in gastric cancer. METHODS: ATP production was measured by ATP Assay kit. Glucose and glutamine uptake were measured by Glucose (HK) Assay Kit and Glutamine/Glutamate Determination Kit respectively...
December 11, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
Jun Shen, Li Liu, Facai Zhang, Jiang Gu, Guanghui Pan
OBJECTIVE: To explore the possible mechanism of lncRNA TapSAKI in urine derived sepsis-induced kidney injury. MATERIALS AND METHODS: In vivo urine-derived sepsis (US) rat model and in vitro LPS-induced HK-2 cells were established, and TapSAKI, miR-22, PTEN, TLR4 and p-p65 expressions were detected by qRT-PCR and western blot. RNA precipitation and RNA pull-down were performed to confirm the interaction between TapSAKI and miR-22. RESULTS: TapSAKI was up-regulated, miR-22 was down-regulated, PTEN, TLR4 and p-p65 expressions, and inflammatory factors TNF-α and IL-6 levels were up-regulated in kidney tissue of US rats and LPS-induced HK-2 cells...
January 21, 2019: Journal of Pharmacy and Pharmacology
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