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Thymic epithelial

Jonathan W Riess, Christina S Kong, Robert B West, Sukhmani K Padda, Joel W Neal, Heather A Wakelee, Quynh-Thu Le
INTRODUCTION: Thymic epithelial tumors (TET) are rare malignancies with a paucity of data on biology and therapeutics. Galectin-1 is a member of the β-galactoside binding protein family and has been shown to mediate tumor growth via modulation of immune cell function. This study examined galectin-1 expression in TET. PATIENTS AND METHODS: A tissue microarray of 68 patients with TET and 8 benign thymus controls were stained for galectin-1 expression and scored by a pathologist blinded to patient clinical and pathologic data...
December 21, 2018: Clinical Lung Cancer
Bryce D Warren, Soo H Ahn, Lynda K McGinnis, Geoffrey Grzesiak, Ren-Wei Su, Asgi T Fazleabas, Lane K Christenson, Brian K Petroff, Margaret G Petroff
Autoimmune Regulator (AIRE) regulates central immune tolerance by inducing expression of tissue-restricted antigens in thymic medullary epithelial cells, thereby ensuring elimination of autoreactive T cells. Aire mutations in humans and targeted Aire deletion in mice results in multi-organ autoimmune disease, known in humans as Autoimmune Polyglandular Syndrome Type 1 (APS-1). APS-1 is characterized by presence of adrenal insufficiency, chronic mucosal candidiasis, and/or hypoparathyroidism. Additionally, females often present with gonadal insufficiency and infertility...
February 16, 2019: Biology of Reproduction
Ryosuke Segawa, Mika Shiraki, Shiori Sudo, Kenichi Shigeeda, Taiji Saito, Natsumi Mizuno, Takahiro Moriya, Takayuki Yonezawa, Je-Tae Woo, Masahiro Hiratsuka, Noriyasu Hirasawa
Thymic stromal lymphopoietin (TSLP) is a key epithelial-derived factor that aggravates allergic diseases. Therefore, TSLP inhibitors are candidate compounds for the treatment of allergic diseases. Previously, we reported that KCMH-1, a mouse keratinocyte cell line, constitutively produces TSLP. In this study, we tried to identify inhibitors of TSLP by screening 2169 compounds in KCMH-1 cells and found one such chalcone derivative (code no. 16D10). 16D10 inhibited TSLP expression and TSLP promoter activation in HaCaT cells, a human keratinocyte cell line...
February 9, 2019: European Journal of Pharmacology
Darin B White, Megan J Hora, Sarah M Jenkins, Randolph S Marks, Yolanda I Garces, Stephen D Cassivi, Anja C Roden
OBJECTIVES: The aim of this study is to evaluate the efficacy of chest computed tomography (CT) to predict the pathological stage of thymic epithelial tumours (TET) using the recently introduced tumour, node and metastasis (TNM) staging with comparison to the modified Masaoka staging. METHODS: Preoperative chest CT examinations in cases of resected TET with sampled lymph nodes (2006-2016) were retrospectively reviewed by 2 thoracic radiologists and radiologically (r) staged using both staging systems...
February 11, 2019: European Journal of Cardio-thoracic Surgery
Xiu-Long Feng, Xue-Bin Lei, Wen-Ting Dong, Lin-Feng Yan, Yong-Kang Xin, Gang-Feng Li, Yong Jing, Shi-Jun Duan, Jie Zhang, Yu-Chuan Hu, Bo Li, Sha-Sha Zhao, Qian Sun, Jin Zhang, Tao Zhang, Dong-Liang Cheng, Guang-Bin Cui, Wen Wang
Background: Thymic epithelial tumors (TETs) are the most common primary thymus tumors, but neither the possible ethnical/regional differences in the incidence of TETs nor the inter-relationships among the clinical variables has been revealed in northwest China. Methods: A retrospective chart review was performed among pathologically confirmed TET patients from January 2004 to December 2015 in a tertiary general hospital of northwest China and the incidence, clinical features and the inter-relationships among clinical variables were analyzed...
December 2018: Journal of Thoracic Disease
Yi Wei, Renqiang Ma, Jia Zhang, Xingmei Wu, Guodong Yu, Xianting Hu, Jian Li, Zhuofu Liu, Wendong Ji, Huabin Li, Weiping Wen
Background: Periostin has been shown to be upregulated in chronic rhinosinusitis with nasal polyps (CRSwNP), especially in the CRSwNP patients with asthma. However, the underlying mechanism that how periostin contributes to the polyp genesis remains unclear. Methods: In this study, we collected 63 CRSwNP patients' nasal polyps (NPs) and 25 control subjects' uncinated tissues. The expressions of periostin, thymic stromal lymphopoietin (TSLP), and other proinflammatory cytokines were examined using IHC staining, qRT-PCR, Western blot (WB), ELISA and FACS...
December 2018: Journal of Thoracic Disease
Ayako Fujiwara, Soichiro Funaki, Naoko Ose, Takashi Kanou, Ryu Kanzaki, Masato Minami, Yasushi Shintani
Background: The hemi-clamshell (HCS) approach provides a wide anterior view of the mediastinum as well as outstanding exposure of the pulmonary hilum. Here, we evaluated the utility and outcomes of this approach in cases of advanced thymic malignancy with hilar invasion. Methods: We performed a retrospective analysis of 14 patients with thymic epithelial malignancy surgically resected with an HCS approach. All required lung resection because of suspected pulmonary hilar vessel invasion...
December 2018: Journal of Thoracic Disease
Kiyoshi Sato, Satoshi Fumimoto, Takayuki Kataoka, Yoshio Ichihashi, Kaoru Ochi, Hidetoshi Satomi, Nobuharu Hanaoka, Yoshikatsu Okada, Takahiro Katsumata
Thymomas are tumors originating from the thymus epithelial cells and are the most common tumors of the anterior mediastinum. They have been classified into types A, AB, B1, B2, and B3 by the World Health Organization. Type B3 thymoma is composed of epithelial cell sheets with mild to moderate atypia and scant lymphocytes. An association between thymic carcinoma and neuroendocrine differentiation has been observed by some authors. However, cases of type B3 thymoma with neuroendocrine differentiation are very rarely discussed in the literature...
2019: SAGE Open Medical Case Reports
Miho Sekai, Jianwei Wang, Nagahiro Minato, Yoko Hamazaki
A clonogenic assay system for thymic epithelial cells (TECs) is of crucial importance for identifying thymic epithelial stem and/or progenitor cells, evaluating their activities, and understanding the mechanisms of thymic involution. However, current systems are not sufficiently sensitive at detecting and quantifying TEC colonies from the adult thymus. Here, we optimized the culture condition to detect visible colonies from adult TECs by modifying our previous culture methods. Epidermal growth factor and leukemia inhibitory factor significantly enhanced the colony-forming efficiency of total TECs from embryo as well as adult mice when added 3 days after plating...
February 6, 2019: Journal of Immunological Methods
Chiaki Nagai-Okatani, Misugi Nagai, Takashi Sato, Atsushi Kuno
Lectin microarray (LMA) is a highly sensitive technology used to obtain the global glycomic profiles of endogenous glycoproteins in biological samples including formalin-fixed paraffin-embedded tissue sections. Here, we describe an effective method for cell type-selective glycomic profiling of tissue fragments collected by laser microdissection (LMD) under fluorescent histochemical visualization. We optimized each step of histochemical staining and confirmed the reliability and validity of glycomic profiling...
February 6, 2019: International Journal of Molecular Sciences
Dana Dohr, Robby Engelmann, Brigitte Müller-Hilke
OBJECTIVE: The central mechanism for establishing a self-tolerant and functional T cell repertoire includes the promiscuous expression of otherwise tissue-restricted proteins by medullary thymic epithelial cells (TEC). We here demonstrate a novel and highly efficient method for isolating this rare key cell type. METHODS: We combined the enrichment of medullary TEC via UEA-1 MicroBeads with the subsequent depletion of residual CD45+ hematopoietic cells via specific size exclusion and compared our results to the standard Percoll enrichment method and isolation procedure via flow cytometric cell sorting...
February 5, 2019: Journal of Immunological Methods
Danjouma Housmanou Cheufou, Daniel Valdivia, Stephan Puhlvers, Benjamin Fels, Gerhard Weinreich, Christian Taube, Dirk Theegarten, Martin Stuschke, Martin Schuler, Balazs Hegedus, Georgios Stamatis, Clemens Aigner
BACKGROUND: Thymic epithelial and neuroendocrine carcinomas are rare malignancies and only few prognosticators are defined. Surgery is the mainstay of treatment and complete resection contributes to superior outcome. Systematic lymph node dissection is not routinely performed in thymic malignancies. Aim of this study was to assess the impact of histologically confirmed lymph node metastases on the outcome following thymectomy. METHODS: We identified 53 patients with thymic epithelial or neuroendocrine carcinomas who underwent surgical resection at our center between 1999 and 2016...
February 2, 2019: Annals of Thoracic Surgery
R Kouitcheu, R Appay, M Diallo, L Troude, A Melot
Thymic epithelial tumors (TET) are rare lesions. The brain metastases of these tumors are even rarer. We report a case of brain metastases in a known patient with a thymic carcinoma diagnosed in October 2016. She was a 73-year-old woman who presented with headache, nausea, and right hemiplegia. Brain MRI revealed five lesions (1 insular, 1 frontal and 2 left temporal, 1 right parafalcine). These lesions were initially treated using two stereotactic radiosurgery gamma knives. A macroscopically complete excision of the left frontal lesion was subsequently performed without any complications with a good evolution of the neurological symptoms postoperatively...
January 30, 2019: Neuro-Chirurgie
Jinquan Li, Yushan Chen, Qiao Yi Chen, Dan Liu, Lang Xu, Guirong Cheng, Xu Yang, Zhenzhong Guo, Yan Zeng
Around the globe, worsening air pollution is spawning major public health and environmental concerns, especially in the poorest and most populous cities. As a major secondary air pollutant, ozone is a potential risk factor for exacerbated asthma, although the underlying mechanisms remain uncertain. In this study, we aim to investigate the role of ozone on asthma exacerbation using a classic asthmatic model with allergic airway inflammation by treating Balb/c mice with ovalbumin (OVA). Our study shows ozone exposure significantly exacerbated OVA-induced asthmatic phenotypes, including serum immunoglobulin, Th cytokines, inflammatory cell counts, mucus production, airway remodeling, and airway hyper-responsiveness (AHR)...
January 24, 2019: Environmental Pollution
Shigeko Kijimoto-Ochiai, Keiko Kamimura, Toshiaki Koda
Neu-medullocytes, which were previously identified and named by our group, are sialidase (neuraminidase)-positive B cells that express immunoglobulin and Mac-1 in the mouse thymus. Recently, B cells that migrated into the thymus were reported to express autoimmune regulator (AIRE) and to contribute to self-tolerance. We sought to determine whether Neu-medullocytes also express AIRE. We obtained positive results by triple staining Neu-medullocytes for in situ sialidase activity, anti-AIRE, and either anti-IgG or anti-IgM antibodies and observing the staining with confocal microscopy...
January 29, 2019: Scientific Reports
K C Lakshmaiah, Tamojit Chaudhuri, Govind K Babu, Lokanatha Dasappa, Linu Abraham Jacob, M C Suresh Babu, A H Rudresha, K N Lokesh, L K Rajeev
BACKGROUND: Thymic epithelial tumors (TET) are the most common tumors of the anterior mediastinum. Patients with advanced/metastatic disease are usually treated with palliative chemotherapy (CT). Unfortunately, even though various palliative CT regimens have been used for long time, there is a real scarcity of published Indian data regarding the experience of palliative CT in metastatic TET (mTET). MATERIALS AND METHODS: This is a retrospective analysis of mTET patients treated between January 2010 and September 2017...
July 2018: Indian Journal of Cancer
Ildikó Bódi, Krisztina H-Minkó, Zsolt Prodán, Nándor Nagy, Imre Oláh
The classical histological features of the thymus are the cortex and medulla, the Hassall's bodies as well as the lobules. Anti-pan-cytokeratin immunocytochemistry shows that the keratin staining pattern of the cortical and medullary epithelial cells is different. The medulla is further compartmentalized: it consists of keratin-positive network and keratin-negative areas. Histology of the keratin-negative area is identical with the connective tissue of the septae. The basal lamina is continuous at the capsule and septae, but it becomes discontinuous at the border between the keratin-positive network and keratin-negative area...
February 2019: Orvosi Hetilap
Xiang Yu, Kaiming Zeng, Xing Liu, Yi Peng, Chunhua Li, Ke Fu, Hui Liang
Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine expressed in the skin, gut, lungs, and thymus. TSLP triggers dendritic cell-mediated T helper 2 inflammatory responses by formation of a ternary complex consisting of a heterodimer of interleukin-7 (IL-7) receptor α chain (IL-7Rα), TSLP, and the TSLP receptor chain (TSLPR). The present study aimed to investigate the expression of this ternary complex and its interaction with signal transducer and activator of transcription 5 (STAT5) in a ischemic stroke model using middle cerebral artery occlusion...
January 22, 2019: Neuroreport
Kei Matsuno, Norihiro Harada, Sonoko Harada, Tomohito Takeshige, Ayako Ishimori, Yukinari Itoigawa, Yoko Katsura, Yuzo Kodama, Fumihiko Makino, Jun Ito, Ryo Atsuta, Hisaya Akiba, Kazuhisa Takahashi
AIM OF THE STUDY: In patients with asthma, chronic inflammatory processes and the subsequent remodeling of the airways contribute to the symptoms and the pathophysiological changes. Epithelial-mesenchymal transition (EMT) is thought to play an important role in tissue remodeling. Previous reports show that tumor necrosis factor (TNF)-like weak inducer of apoptosis (TWEAK) is a cytokine of the TNF superfamily, exerts pro-inflammatory effects, and enhances transforming growth factor (TGF)-β-induced EMT in bronchial epithelial cells...
January 24, 2019: Experimental Lung Research
Jie Zheng, Xiaoyu Wang, Yu Tao, Yan Wang, Xi Yu, Hailiang Liu, Lv Ji, Kaifan Bao, Can Wang, Zhirong Jia, Min Hong
BACKGROUND: Atopic dermatitis (AD) is a common allergic inflammatory skin disease, concomitant with a high relapse rate. Yu-Ping-Feng-San (YPFS), a well-known Chinese herbal decoction, reduces the AD relapse rate and recurring severity incidence. However, the underlying mechanism of YPFS on resisting AD recurrence is still unknown and further study is needed. PURPOSE: To evaluate the effects of YPFS on recurrent allergic inflammation of AD in a murine model and to investigate the underlying mechanisms in vivo and ex vivo...
September 19, 2018: Phytomedicine: International Journal of Phytotherapy and Phytopharmacology
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