Sarfaraz Ahmad, Gagan Deep, Henry A Punzi, Yixin Su, Sangeeta Singh, Ashish Kumar, Shalini Mishra, Amit K Saha, Kendra N Wright, Jessica L VonCannon, Louis J Dell'Italia, Wayne J Meredith, Carlos M Ferrario
BACKGROUND: Extracellular vesicles (EVs) have emerged as a promising liquid biopsy for various diseases. For the first time, using plasma and urinary EVs, we assessed the activity of renin-angiotensin system (RAS), a central regulator of renal, cardiac, and vascular physiology, in patients with control (Group I) or uncontrolled (Group II) primary hypertension. METHODS: EVs were isolated from 34 patients with history of hypertension, and characterized for size and concentration by nanoparticle tracking analyses, exosomal biomarkers by immunogold labeling coupled with transmission electron microscopy, flow cytometry and immunoblotting...
November 10, 2023: medRxiv