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follicle development

Haisheng Ding, Huiling Zhao, Guanglong Cheng, Yongxin Yang, Xiaofei Wang, Xiaowei Zhao, Yunxia Qi, Dongwei Huang
BACKGROUND: Hair fibre length is an important economic trait of rabbits in fur production. However, molecular mechanisms regulating rabbit hair growth have remained elusive. RESULTS: Here we aimed to characterise the skin traits and gene expression profiles of short-hair and long-hair rabbits by histological and transcriptome analyses. Haematoxylin-eosin staining was performed to observe the histological structure of the skin of short-hair and long-hair rabbits...
February 15, 2019: BMC Genomics
G Brambila-Souza, C C Mylonas, P H Mello, R Y Kuradomi, S R Batlouni, C E Tolussi, R G Moreira
Lambari-do-rabo-amarelo Astyanax altiparanae in the wild reproduce during spring and summer, but females undergo vitellogenesis throughout the year, including the non-spawning winter period when water temperatures are low. The present study investigated the physiological role of temperature modulation on the hypothalamus-pituitary-gonads axis of lambari during winter, as well as the effects of gonadotropin releasing hormone agonist (GnRHa) therapy. Captive females were exposed to two different temperatures (20°C and 27°C) and were injected weekly with GnRHa for 21 days during winter (Control, CTR; Low dose; LD and high dose of GnRHa, HD)...
February 12, 2019: General and Comparative Endocrinology
Yudong Jia, Jilin Lei
Gonadotropins (GtHs) and their receptors (follicle-stimulating hormone receptor, FSHR; luteinizing hormone receptor, LHR) are involved in the regulation of gametogenesis and play important roles during the reproductive cycles in vertebrate species, including fish. This minireview focuses on the molecular characterization and quantification of GtHs (common glycoprotein α subunit CGα, FSHβ, and LHβ) and their receptors (FSHR and LHR) throughout the reproductive cycle of female turbot Scophthalmus maximus...
February 12, 2019: General and Comparative Endocrinology
Daranporn Triwongwaranat, Rattapon Thuangtong, Sittiroj Arunkajohnsak
Hair pigmentation is regulated by follicular melanogenesis, in which the process consists of melanin formation and transfer to keratinocytes in the hair shaft. Human hair follicles contain two types of melanin: the brown-black eumelanin and yellow-red pheomelanin. Eumelanin is commonly present in black and brown hair while pheomelanin is found in auburn and blonde hair. Hair follicle melanogenesis is under cyclical control and is concurrently coupled to hair growth. Many factors including intrinsic and extrinsic factors affect the follicular melanogenesis...
February 15, 2019: International Journal of Dermatology
Genoa R Warner, Zhong Li, Madeline L Houde, Cassandra E Atkinson, Daryl D Meling, Catheryne Chiang, Jodi A Flaws
Phthalates are synthetic chemicals with widespread human exposure due to their use as additives in consumer products. Phthalate diesters are hydrolyzed in the environment and in the body to monoesters that may be more toxic than the parent compounds. This study tested the hypothesis that adult mouse antral follicles, but not neonatal ovaries, are able to metabolize an environmentally relevant mixture of phthalates. Whole neonatal ovaries and isolated adult antral follicles from CD-1 mice were cultured in media treated with vehicle control or 0...
February 15, 2019: Toxicological Sciences: An Official Journal of the Society of Toxicology
José María Fernández-Santos, José Carmelo Utrilla, Victoria Vázquez-Román, José Luis Villar-Rodríguez, Lorenzo Gutiérrez-Avilés, Inés Martín-Lacave
BACKGROUND: Primary cilia (PC) are conserved structures in the adult thyroid gland of different mammals. We have recently described that, in humans, PC are usually present as a single copy per follicular cell emerging from the follicular cell apex into the follicular lumen. METHODS: To better understand the role developed by PC in thyroid hormonogenesis, we investigated their changes in different human functional thyroid diseases (diffuse toxic hyperplasia/Graves' disease and nodular hyperplasia/nodular goiter), in comparison with normal thyroid tissue, using immunofluorescence, morphometry and electron microscopy analyses...
February 15, 2019: Thyroid: Official Journal of the American Thyroid Association
Chang Liu, Wenchao Sun, Guangchao Zhuo, Zhifen Zhang
In mammalian follicles, oocytes are arrested at the diplotene stage of prophase I until meiotic resumption following the LH surge. C-type natriuretic peptide (CNP), encoded by natriuretic peptide precursor type C (NPPC), was found to be reduced by the LH surge in the follicle, and then lead to meiotic resumption by decreasing the level of cAMP in the oocyte. As a wide-spread cytokine, macrophage colony-stimulating factor (M-CSF) takes part in the oocyte development to maturation and ovulation. Our study describes the expression curve of M-CSF and its receptor and investigates the impact on the levels of CNP/NPPC to explore the possible mechanism for meiotic resumption in both vivo and vitro...
February 15, 2019: Gynecological Endocrinology
Salvador Hernández-Martínez, Víctor Cardoso-Jaime, Marcela Nouzova, Veronika Michalkova, Cesar E Ramirez, Francisco Fernandez-Lima, Fernando G Noriega
Anophelinae mosquitoes are vectors of human malaria, a disease that infects hundreds of millions of people and causes almost 600,000 fatalities annually. Despite their medical importance, laboratory studies on key aspects of Anophelinae reproductive biology have been limited, and in particular, relatively little is known about the role of juvenile hormone (JH) in the control of female reproduction. The study presented here attempts to fill a gap of knowledge in our understanding of the JH control of ovarian development in female Anophelinae mosquitoes, using Anopheles albimanus as a model...
February 14, 2019: Scientific Reports
V R P Barros, A P O Monte, T L B G Lins, J M Santos, V G Menezes, A Y P Cavalcante, V R Araújo, B B Gouveia, M H T Matos
This study evaluated the in vitro development and maturation of ovine oocytes from secondary follicles cultured in serum-free medium containing fixed or sequential concentrations of recombinant human FSH (rhFSH). Follicles were cultured in α-MEM+ alone or with constant (500, 750, or 1,000 ng/mL) or sequential concentrations of rhFSH (seq. 1: day 6 = 500; day 12 = 750; day 18 = 1,000 ng/mL and seq. 2: day 6 = 100; day 12 = 500; day 18 = 1,000 ng/mL). At the end of the experiment, follicular survival was higher (P < 0...
December 18, 2018: Domestic Animal Endocrinology
Aoife Parsons, Anke Lange, Thomas H Hutchinson, Shinichi Miyagawa, Taisen Iguchi, Tetsuhiro Kudoh, Charles R Tyler
Brominated flame retardants are known to disrupt thyroid hormone (TH) homeostasis in several vertebrate species, but the molecular mechanisms underlying this process and their effects on TH-sensitive tissues during the stages of early development are not well characterised. In this study, we exposed zebrafish (Danio rerio) embryo-larvae to 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) and tetrabromobisphenol A (TBBPA) via the water for 96 h from fertilisation and assessed for lethality, effects on development and on the expression of a suite of genes in the hypothalamic-pituitary-thyroid (HPT) axis via both real time quantitative PCR (qRT-PCR) on whole body extracts and whole mount in situ hybridisation (WISH) to identify tissue targets...
January 28, 2019: Aquatic Toxicology
Stella Campo, Luz Andreone, Verónica Ambao, Mariela Urrutia, Ricardo S Calandra, Susana B Rulli
The Follicle-Stimulating Hormone plays an important role in the regulation of gametogenesis. It is synthesized and secreted as a family of glycoforms with differing oligosaccharide structure, biological action, and half-life. The presence of these oligosaccharides is absolutely necessary for the full expression of hormone bioactivity at the level of the target cell. The endocrine milieu modulates the glycosylation of this hormone. During male sexual development a progressive increase in FSH sialylation and in the proportion of glycoforms bearing complex oligosaccharides are the main features in this physiological condition...
2019: Frontiers in Endocrinology
Thomas Keith Cunningham, Victoria Allgar, Soha R Dargham, Eric Kilpatrick, Thozhukat Sathyapalan, Stephen Maguiness, Haira R Mokhtar Rudin, Nour M Abdul Ghani, Aishah Latiff, Stephen L Atkin
Objective: The relationship between fertilization rates and 1,25-dihydroxyvitamin D (1,25(OH)2 D3 ), 25-hydroxyvitamin D2 (25(OH)D2 ), 25-hydroxyvitamin D3 (25(OH)D3 ), 24,25-dihydroxyvitamin D (24,25(OH)2 D3 ), and 25-hydroxy-3epi-Vitamin D3 (3epi25(OH)D3 ) concentrations in age and weight matched women with and without PCOS was studied. Methods: Fifty nine non-obese women, 29 with PCOS, and 30 non-PCOS undergoing IVF, matched for age and weight were included. Serum vitamin D metabolites were taken the menstrual cycle prior to commencing controlled ovarian hyperstimulation...
2019: Frontiers in Endocrinology
Young-Cheol Lim, Hyeongi Kim, Sang Moo Lim, Jin Su Kim
BACKGROUND: Chemotherapy-induced alopecia has been well documented as a cause of distress to patients undergoing cancer treatment. Almost all traditional chemotherapeutic agents cause severe alopecia. Despite advances in the treatment of chemotherapy-induced alopecia, there is no effective treatment for preventing chemotherapy-induced alopecia. METHODS: In the present study, we investigated the potential role of a multi-target iron chelator, M30 in protecting against cyclophosphamide-induced alopecia in C57BL/6 mice implanted with an osmotic pump...
February 13, 2019: BMC Cancer
Prapti Singh, Ariela Agress, Vanessa Madrigal, Clara Magyar, Nora Ostrzega, Gregorio Daniel Chazenbalk, Daniel Anthony Dumesic
Context: Ovarian hyperandrogenism from polycystic ovarian syndrome (PCOS) and hyperinsulinemia from insulin resistance are modulators of ovarian follicle development. We report a PCOS woman with hyperandrogenism and severe insulin resistance from metabolic syndrome who received long-term GnRH analog therapy preceding bilateral salpingo-oophorectomy for massive ovarian enlargement. Ovarian histological examination showed proliferating granulosa cells within antral follicles coexistent with serous cystadenofibromas, demonstrating a unique link between hyperinsulinemia and granulosa cell mitogenesis...
February 12, 2019: Journal of Clinical Endocrinology and Metabolism
Minh Binh Nguyen, Victor Julian Valdes, Idan Cohen, Venu Pothula, Dejian Zhao, Deyou Zheng, Elena Ezhkova
Merkel cells are mechanosensory cells involved in tactile discrimination. Merkel cells have been primarily studied in the murine back skin, where they are found in specialized structures called touch domes located around primary hair follicles. Yet, little is known about the morphogenesis of Merkel cells in areas of the skin devoid of hair, such as the glabrous paw skin. Here, we describe Merkel cell formation in the glabrous paw skin during embryogenesis. We first found in the glabrous paw skin that Merkel cells were specified at E15...
February 13, 2019: Experimental Dermatology
Ahmed Gad, Lucie Nemcova, Matej Murin, Jiri Kanka, Jozef Laurincik, Michal Benc, Lazo Pendovski, Radek Prochazka
Oocyte developmental competence is acquired during folliculogenesis and regulated by complex molecular mechanisms. Several molecules are involved in these mechanisms, including microRNAs (miRNAs) that are essential for oocyte-specific processes throughout the development. The objective of this study was to identify the expression profile of miRNAs in porcine oocytes derived from follicles of different sizes using RNA deep sequencing. Oocytes were aspirated from large (LO; 3-6 mm) or small (SO; 1.5-1.9 mm) follicles and tested for developmental competence and chromatin configurations...
February 13, 2019: Molecular Reproduction and Development
Zhujun Gu, Yizhong Wang, Kai Lin, Xing Wang, Weiwei Cheng, Ling Wang, Ting Zhang, Haifeng Liu
OBJECTIVE: Capsule endoscopy (CE) is a noninvasive diagnostic tool for the digestive tract. We aim to investigate the feasibility and safety of newly-developed magnetically controlled capsule endoscopy (MCE) in children. METHODS: A total of 129 children who underwent MCE in Shanghai Children's Hospital were retrospectively recruited between March 2016 and August 2018. The feasibility, positive findings, and safety of MCE were evaluated and systematically analyzed...
February 7, 2019: Journal of Pediatric Gastroenterology and Nutrition
Liyan Pan, Guangjian Liu, Xiaojian Mao, Huixian Li, Jiexin Zhang, Huiying Liang, Xiuzhen Li
BACKGROUND: Central precocious puberty (CPP) in girls seriously affects their physical and mental development in childhood. The method of diagnosis-gonadotropin-releasing hormone (GnRH)-stimulation test or GnRH analogue (GnRHa)-stimulation test-is expensive and makes patients uncomfortable due to the need for repeated blood sampling. OBJECTIVE: We aimed to combine multiple CPP-related features and construct machine learning models to predict response to the GnRHa-stimulation test...
February 12, 2019: JMIR Medical Informatics
Jennifer Gherardini, Jeannine Wegner, Jérémy Chéret, Sushmita Ghatak, Janin Lehmann, Majid Alam, Francisco Jimenez, Wolfgang Funk, Markus Böhm, Natalia Botchkareva, Chris Ward, Ralf Paus, Marta Bertolini
OBJECTIVES: While the effect of ultraviolet radiation (UVR) on human skin has been extensively studied, very little is known on how UVR impacts on hair follicle (HF) homeostasis. Here, we investigated how solar spectrum UVR that hits the human skin surface impacts on HF biology, and whether any detrimental effects can be mitigated by a widely used cosmetic and nutraceutical ingredient, caffeine. METHODS: Human scalp skin with terminal HFs was irradiated transepidermally ex vivo using either 10 J/cm2 UVA (340-440 nm) + 20 mJ/cm2 UVB (290-320 nm) (low dose) or 50 J/cm2 UVA + 50 mJ/cm2 UVB (high dose) and organ-cultured under serum-free conditions for 1 or 3 days...
February 11, 2019: International Journal of Cosmetic Science
Maasoume Abdollahi, Mojdeh Salehnia, Saghar Salehpour, Shahram Pour-Beiranvand
Background: For improving the human ovarian tissue culture, this study was designed to assess the incidence of apoptosis in this tissue following vitrification and in vitro culture in the presence of leukemia inhibitory factor (LIF) as an anti-apoptotic factor. Methods: After collecting the ovarian tissue samples they were divided into non-vitrified and vitrified groups and cultured for 14 days in the presence and absence of LIF then morphological, ultrastructural and steroidogenesis studies, TUNEL and caspase-3/7 assays, and apoptosis analysis by real time RT-PCR were done in all groups...
October 2018: Journal of Reproduction & Infertility
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