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Collagen degradation

Tanya Tyagi, May Alarab, Yvonne Leong, Stephen Lye, Oksana Shynlova
This study investigates the effect of local oestrogen therapy (LET) on the expression of proteins participating in collagen/elastin biogenesis and immune markers in vaginal tissues of post-menopausal women with severe pelvic organ prolapse (POP). Vaginal biopsies were collected from the anterior vaginal wall of informed and consented 52 post-menopausal women with severe POP undergoing total hysterectomy. Twenty-nine of the 52 women were treated with LET (in the form of vaginal oestrogen cream or tablet), while the remaining 23 untreated patients served as the controls...
February 17, 2019: Journal of Cellular and Molecular Medicine
Andrés Vásquez-Rivera, Harriëtte Oldenhof, Andres Hilfiker, Willem F Wolkers
Decellularized heart valves hold promise for their use as bioscaffolds in cardiovascular surgery. Quality assessment of heart valves after decellularization processing and/or storage is time consuming and destructive. Fourier transform infrared spectroscopy (FTIR) allows rapid non-invasive assessment of biomolecular structures in tissues. In this study, IR-spectra taken from different layers of the pulmonary artery trunk and leaflet tissues of decellularized porcine heart valves were compared with those of pure collagen and elastin, the main protein components in these tissues...
February 5, 2019: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Heikkinen Anne, Härönen Heli, Norman Oula, Pihlajaniemi Taina
Alongside playing structural roles, the extracellular matrix (ECM) acts as an interaction platform for cellular homeostasis, organ development, and maintenance. The necessity of the ECM is highlighted by the diverse, sometimes very serious diseases that stem from defects in its components. The neuromuscular junction (NMJ) is a large peripheral motor synapse differing from its central counterparts through the ECM included at the synaptic cleft. Such synaptic basal lamina (BL) is specialized to support NMJ establishment, differentiation, maturation, stabilization, and function and diverges in molecular composition from the extrasynaptic ECM...
February 15, 2019: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Akihiro Nishiguchi, Tetsushi Taguchi
An injectable bone may serve as a minimally invasive therapy for large orthopedic defects and osteoporosis and an alternative to allografting and surgical treatment. However, conventional bone substitutes lack the desirable biodegradability, bioresponsibility, and functionality to regulate the bone regeneration process. Here, we report an injectable, bioresponsive bone composed of bisphosphonate-modified nanocellulose (pNC) as a bone substitute for bone regeneration. Composites composed of nano-fibrillated cellulose and β-tricalcium phosphate (β-TCP) mimic bone structures in which apatite reinforces collagen fibrils...
February 15, 2019: Biomacromolecules
Jadwiga Wańczyk-Baszak, Sławomir Woźniak, Bartosz Milejski, Tomasz Paszkowski
Perimenopausal changes caused by oestrogen deficiency are accompanied by a decrease in the content of collagen and elastin in the tissues, leading to thinning of the epithelium and the resultant disappearance of the superficial layer, which leads to smooth muscle dysfunction as well as connective tissue degradation. This aetiopathogenetic chain results in a set of symptoms experienced by approximately 50% of women in the peri- and postmenopausal period. Symptoms of dryness, burning, dyspareunia and urgency contribute to a significant reduction in the quality of sexual function and general comfort of life due to recurrent infections of the vagina, vulva and urinary tract...
December 2018: Przeglad Menopauzalny, Menopause Review
Chang Tu, Xiaojian Huang, Yifan Xiao, Mingyu Song, Yongzhuang Ma, Jiyuan Yan, Hongbo You, Hua Wu
Osteoarthritis (OA) is a common joint disease in the elderly population. Its development has been reported to be associated with cartilage degradation and inflammatory responses. Schisandrin A, a bioactive lignin in Schisandra sphenanthera , has shown its anti-inflammatory potential in various inflammation diseases. However, the effects of Schisandrin A on OA remain to explore. In this study, rat chondrocytes were treated with IL-1β (10 ng/ml) with or without different concentrations of Schisandrin A for 24 h...
2019: Frontiers in Pharmacology
Sreya Das, Srisathya Srinivasan, Ankita Srivastava, Sushant Kumar, Gargi Das, Suman Das, Alka Dwivedi, Atharva Karulkar, Khushi Makkad, Richa Bilala, Ankit Gupta, Abhijeet Sawant, Chitra Nayak, Prakriti Tayalia, Rahul Purwar
T cells mediate skin immune surveillance by secreting specific cytokines and regulate numerous functions of keratinocytes, including migration during homeostasis and disease pathogenesis. Keratinocyte migration is mediated mainly by proteolytic cleavage of the extracellular matrix and/or by cytoskeleton reorganization. However, the cross-talk between T cell cytokines and actomyosin machinery of human primary keratinocytes (HPKs), which is required for cytoskeleton reorganization and subsequent migration, remains poorly examined...
February 13, 2019: Journal of Immunology: Official Journal of the American Association of Immunologists
Xiaoyun Peng, Zhiguang Qiao, You Wang, Huiqin Li, Yilin Xie, Mei Xin, Zhongdong Qiao, Zhaoxia Wang
PROBLEM: Osteoarthritis (OA) is a chronic disease with a very high incidence and the pathology of which is quite complex. Epidemiological investigation showed that OA may be related to smoking and estrogen levels, but there are few studies focused on the cross-effect of these two factors. This research aims to investigate the molecular mechanism of nicotine and estrogen effects on chondrocytes to study the effect of smoking on the incidence of osteoarthritis in women. METHOD OF THE STUDY: Nicotine was added to obtain inflammatory supernatants of macrophages, which were used to induce chondrocyte inflammation...
February 11, 2019: International Journal of Rheumatic Diseases
Hasan Mete Aksoy, Berna Aksoy, Emel Çalikoglu
Systemic retinoids such as isotretinoin and acitretin are associated with numerous side effects but are therapeutically valuable in dermatological practice. Several studies have reported the wound-healing effects of treatment with concurrent systemic retinoids in addition to surgical treatments. Herein, we describe two patients with scar dehiscence that developed after initiation of systemic retinoid treatment following cutaneous surgery. In Case 1, isotretinoin was thought to decrease collagen production to a greater degree than did degradation during the wound remodeling phase, which resulted in wound dehiscence...
January 2019: Indian Journal of Dermatology
M Putzu, F Causa, Manuel Parente, Israel González de Torre, J C Rodriguez-Cabello, P A Netti
In the field of tissue engineering the choice of materials is of great importance given the possibility to use biocompatible polymers produced by means of biotechnology. A large number of synthetic and natural materials have been used to this purpose and processed into scaffolds using Electrospinning technique. Among materials that could be used for the fabrication of scaffold and degradable membranes, natural polymers such as collagen, elastin or fibroin offer the possibility to design structures strictly similar to the extracellular matrix (ECM)...
February 2019: Regenerative Biomaterials
Jennifer Lehmann, Michael Praktiknjo, Mette Juul Nielsen, Robert Schierwagen, Carsten Meyer, Daniel Thomas, Francesco Violi, Christian P Strassburg, Flemming Bendtsen, Søren Møller, Aleksander Krag, Morten Asser Karsdal, Diana Julie Leeming, Jonel Trebicka
BACKGROUND: Remodeling of extracellular matrix (ECM) is crucial in progressive liver fibrosis. Collagen type III desposition has been shown in acute decompensation. ECM is compiled of deposition of various components. The role of basement membrane collagen type IV in advanced liver cirrhosis and acute decompensation is unclear and investigated in this study. METHODS: Patients with decompensated liver cirrhosis from the prospective NEPTUN cohort (ClinicalTrialsgov Identifier: NCT03628807), who underwent TIPS procedure were included...
February 9, 2019: Liver International: Official Journal of the International Association for the Study of the Liver
Zhiheng Dong, Sha Li, Xiaohui Wang, Lengge Si, Ruilian Ma, Lidao Bao, Agula Bo
Hepatic fibrosis is chronic liver damage with many causes that has a relatively high death rate. The current study showed that lncRNA GAS5, miR-23a and PTEN play important roles in the pathological process of hepatic fibrosis but have a relatively unclear regulatory mechanism. This study aimed to investigate the roles of lncRNA GAS5, miR-23a and PTEN in the pathological process of hepatic fibrosis and HSC activation. We used CCl4 intraperitoneal injections to establish a rat hepatic fibrosis model and exogenous TGF-β1 to establish a hepatic stellate cell (HSC) activation model...
February 8, 2019: American Journal of Physiology. Gastrointestinal and Liver Physiology
Luciana Migliore, Nicoletta Perini, Fulvio Mercuri, Silvia Orlanducci, Alessandro Rubechini, Maria Cristina Thaller
The preservation of cultural heritage is one of the major challenges of today's society. Parchments, a semi-solid matrix of collagen produced from animal skin, are a significant part of the cultural heritage, being used as writing material since ancient times. Due to their animal origin, parchments easily undergo biodeterioration: the most common biological damage is characterized by isolated or coalescent purple spots, that often lead to the detachment of the superficial layer and the consequent loss of written content...
February 7, 2019: Scientific Reports
Lin Chen, Ping-Lin He, Jin Yang, Ya-Fei Yang, Kai Wang, Bastian Amend, Arnulf Stenzl, Ya-Mei Zhang, Zi-Li Wang, Sha-Sha Xing, Xu Luo
Bladder dysfunction is associated with fibrosis‑-mediated aging, but the corresponding mechanism remains to be elucidated. Activation of the NACHT, LRR and PYD domains‑containing protein 3 (NLRP3) inflammasome is related to chronic diseases associated with aging, including organ fibrosis. The present study aimed to explore the role of NLRP3/interleukin 1β in aging‑associated bladder dysfunction. Female Sprague‑Dawley rats were divided into the following two groups (n=10 rats/group): 2‑month‑old group (young group) and 24‑month‑old group (old group)...
January 31, 2019: Molecular Medicine Reports
Robert E Seegmiller, Cameron Foster, Jared L Burnham
BACKGROUND: The mutant chondrodysplasia (cho) is a cartilage-targeting disorder in C57BL mice that results in dwarfing and other malformations stemming from this collagenopathy. Clarke Fraser made the discovery of the mutation accidentally in the early 1960s during the thalidomide tragedy. METHODS: For this review we identified key research on cho as since its discovery. Relevant data were compiled to make a comprehensive review that details discoveries associated with the cho mutation, that describes the associated phenotypes and molecular mechanisms, and that provides a discussion surrounding its current clinical relevance...
February 4, 2019: Birth Defects Research
Ji Suk Choi, Woo Lee Cho, Yeo Jin Choi, Jae Dong Kim, Hyun-A Park, Su Yeon Kim, Jae Hyung Park, Dong-Gyu Jo, Yong Woo Cho
Ultraviolet-B (UVB) irradiation causes imbalance between dermal matrix synthesis and degradation through aberrant upregulation of matrix metalloproteinases (MMPs), which leads to overall skin photoaging. We investigated the effects of extracellular vesicles (EVs) derived from human adipose-derived stem cells (HASCs) on photo-damaged human dermal fibroblasts (HDFs). EVs were isolated from conditioned media of HASCs with tangential flow filtration and characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blotting, micro RNA (miRNA) arrays, cytokine arrays and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)...
2019: Journal of Extracellular Vesicles
R Uemura, J Miura, T Ishimoto, K Yagi, Y Matsuda, M Shimizu, T Nakano, M Hayashi
Root caries is an increasingly problem in aging societies with severe implications for the general health and wellbeing of large numbers of people. Strengthening type-I collagen, a major organic component of human dentin, has proved effective in preventing root caries. This study sought to determine whether exposure to riboflavin followed by UVA irradiation (RF/UVA) could promote additional collagen crosslinking, and thus improve the acid and enzymatic resistance of human dentin under simulated oral environments...
February 4, 2019: Scientific Reports
Céline Deroyer, Edith Charlier, Sophie Neuville, Olivier Malaise, Philippe Gillet, William Kurth, Alain Chariot, Michel Malaise, Dominique de Seny
CEMIP (for "Cell migration-inducing protein" also called KIAA1199 and Hybid for "Hyaluronan-binding protein") expression is increased in cancers and described as a regulator of cell survival, growth and invasion. In rheumatoid arthritis, CEMIP is referred to as an angiogenic marker and participates in hyaluronic acid degradation. In this study, CEMIP expression is investigated in healthy and osteoarthritis (OA) cartilage from human and mouse. Its role in OA physiopathology is deciphered, specifically in chondrocytes proliferation and dedifferentiation and in the extracellular matrix remodeling...
February 4, 2019: Cell Death & Disease
Jing Ji, Liqun He
Kangxianling (KXL) decoction is a traditional Chinese herbal formulation which has been used to treat early and midterm chronic renal failure. Renal fibrosis is a common characteristic of progressive chronic kidney diseases (CKD). The formation of renal fibrosis is caused by kidney trauma, infection, and immune response. The pathophysiological mechanism of renal fibrosis was mainly due to increased collagen synthesis in the kidney, decreased degradation, and a large amount of extracellular matrix (ECM) deposition...
2019: Evidence-based Complementary and Alternative Medicine: ECAM
Marina Danalache, Robin Kleinert, Janine Schneider, Anna-Lisa Erler, Maik Schwitalle, Rosa Riester, Frank Traub, Ulf Krister Hofmann
OBJECTIVE: During osteoarthritis, chondrocytes seem to change their spatial arrangement from single to double strings, small and big clusters. Since the pericellular matrix (PCM) appears to degrade alongside this reorganisation, it has been suggested that spatial patterns act as an image-based biomarker for osteoarthritis. The aim of this study was to establish the functional relevance of spatial organisation in articular cartilage. METHOD: Cartilage samples were selected according to their predominant spatial cellular pattern...
January 31, 2019: Osteoarthritis and Cartilage
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