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Umbilical Stem Cell Injection

Boohwi Hong, Sunyeul Lee, Nara Shin, Youngkwon Ko, DongWoon Kim, Jun Lee, Wonhyung Lee
Objectives: Current treatments for osteoporosis were prevention of progression, yet it has been questionable in the stimulation of bone growth. The mesenchymal stem cells (MSCs) treatment for osteoporosis aims to induce differentiation of bone progenitor cells into bone-forming osteoblasts. We investigate whether human umbilical cord blood (hUCB)-MSCs transplantation may induce bone regeneration for osteoporotic rat model induced by ovariectomy. Methods: The ovariectomized (OVX) group (n = 10) and OVX-MSCs group (n = 10) underwent bilateral ovariectomy to induce osteoporosis, while the Sham group (n = 10) underwent sham operation at aged 12 weeks...
September 2018: Osteoporosis and Sarcopenia
Ainara Benavente-Babace, Kristina Haase, Duncan J Stewart, Michel Godin
Endothelial progenitor cells (EPCs) and human mesenchymal stem cells (hMSCs) have shown great regenerative potential to repair damaged tissue; however, their injection in vivo results in low retention and poor cell survival. Early clinical research has focused on cell-encapsulation, to improve viability and integration of delivered cells. However, this strategy has been limited by the inability to reproduce large volumes of standardized microcapsules and the lack of information on cell-specific egress and timed release from hydrogel microcapsules...
February 16, 2019: Journal of Tissue Engineering and Regenerative Medicine
Shashank Shankar Chetty, Selvarasu Praneetha, Kavitha Govarthanan, Rama Shanker Verma, Arumugam Vadivel Murugan
Acute liver injury is a critical syndrome ascribed to prevalent death of hepatocyte and imperatively requires a liver transplantation. Such methodology is certainly hampered due to deficit of healthy donors. In this regard, stem cells-based regenerative therapies are attractive in repairing injured tissues and organs for medical applications. However, it is crucial to understand the migration, engraftment and regeneration capabilities of transplanted stem cells in the living animal models. For the first time, we demonstrate rapid labeling of umbilical cord-derived mesenchymal stem cells (MSCs) with near-infrared (NIR) fluorescent CuInS2-ZnS nanocrystals (CIZS-NCs) to develop an innovative nano-bioconjugates (MSCs-CIZS-NBCs) that exhibit 98% labeling efficiency...
February 11, 2019: ACS Applied Materials & Interfaces
Yanfu Han, Tianjun Sun, Yanqing Han, Lingling Lin, Chang Liu, Jing Liu, Guangzhi Yan, Ran Tao
OBJECTIVE: Difficulty in wound healing is one common complication of diabetes mellitus. The study explored whether the therapeutic effect of human umbilical cord mesenchymal stem cells (hUCMSCs) on diabetic ulcer wound was enhanced by the activation of the Wnt signaling pathway. METHODS: Rat diabetic model was established by intraperitoneal injection of Streptozotocin (STZ). hUCMSCs were purified and seeded on the collagen-chitosan laser drilling acellular dermal matrix (CCLDADM) scaffold, which was subsequently implanted into the cutaneous wound of normal and diabetic rats, followed by daily injection of Wnt signaling pathway agonist (Wnt3a) or antagonist (sFRP3) at the edge of the scaffold...
February 8, 2019: European Journal of Medical Research
Asma Alanazi, Hafsa Munir, Mohammed Alassiri, Lewis S C Ward, Helen M McGettrick, Gerard B Nash
BACKGROUND: Mesenchymal stem cells (MSC) are used in therapy, often by injection into the blood. OBJECTIVE: We aimed to compare the adhesive and migratory properties of MSC from umbilical cords (UCMSC), bone marrow (BMMSC) or trabecular bone (TBMSC), which might influence delivery to injured tissue. METHODS: MSC were perfused through glass capillaries coated with matrix proteins, collagen or fibronectin, or albumin. Adherent cells were counted microscopically and their spreading analysed over time...
January 24, 2019: Biorheology
Cheng-Hsien Lin, Willie Lin, Yu-Chin Su, Yogi Cheng-Yo Hsuan, Yu-Chien Chen, Ching-Ping Chang, Willy Chou, Kao-Chang Lin
BACKGROUND & PURPOSE: Following traumatic brain injury (TBI), primary mechanical injury to the brain may cause blood-brain-barrier damage followed by secondary injury, ultimately culminating in cell death. We aimed to test whether one injection of mesenchymal stem cells (MSC) derived from the human umbilical cord can modulate brain cytokine and chemokine gene profiles and attenuate neurological injury in rats with TBI. METHODS: One-day post-TBI, the injured rats were treated with one injection of MSC (4 × 106 /rat, i...
January 29, 2019: Journal of the Formosan Medical Association, Taiwan Yi Zhi
Vaishnevi Raj, Stephanie Claudine, Arjunan Subramanian, Kimberley Tam, Arijit Biswas, Ariff Bongso, Chui-Yee Fong
We showed in previous studies that human umbilical cord Wharton's jelly stem cells (hWJSCs) improved the healing rates of excisional and diabetic wounds in the mouse model. As an extension of those studies, we report here the more detailed quantitative histological, immunohistochemical, and genomic evaluation of biopsies from those excisional and diabetic wounds in an attempt to understand the mechanisms of the enhanced wound healing aided by hWJSCs. Bright-field microscopic observations and ImageJ software analysis on histological sections of the excisional and diabetic wound biopsies collected at different time points showed that the thickness of the epidermis and dermis, and positive picrosirius-red stained areas for collagen, were significantly greater in the presence of hWJSCs compared with controls (P < 0...
January 31, 2019: Journal of Cellular Biochemistry
Sen Ren, Jing Chen, Dominik Duscher, Yutian Liu, Guojun Guo, Yu Kang, Hewei Xiong, Peng Zhan, Yang Wang, Cheng Wang, Hans-Günther Machens, Zhenbing Chen
BACKGROUND: Human adipose stem cells (ASCs) have emerged as a promising treatment paradigm for skin wounds. Recent works demonstrate that the therapeutic effect of stem cells is partially mediated by extracellular vesicles, which comprise exosomes and microvesicles. In this study, we investigate the regenerative effects of isolated microvesicles from ASCs and evaluate the mechanisms how ASC microvesicles promote wound healing. METHODS: Adipose stem cell-derived microvesicles (ASC-MVs) were isolated by differential ultracentrifugation, stained by PKH26, and characterized by electron microscopy and dynamic light scattering (DLS)...
January 31, 2019: Stem Cell Research & Therapy
Xiao Chen, Kangwu Wang, Shijun Chen, Yuqing Chen
Interferon-β (IFN-β) exhibits a tumor-killing effect; however, injection of IFN-β alone for lung cancer is often accompanied by side effects. This study investigated the possibility of using umbilical cord mesenchymal stem cells (MSCs) as cellular carriers of IFN-β. Isolated umbilical cord MSCs were transfected with a lentivirus packaging IFN-β-overexpression plasmid. A549 cells were subcutaneously injected into nude mice to establish a non-small cell lung cancer (NSCLC) mouse model. A total of 50 mice were randomly assigned to 5 different groups: a control group, IFN-β group, IFN-β-MSCs group, MSCs-lentivirus group, and MSCs group...
January 14, 2019: Pathology, Research and Practice
Chenggui Wang, Min Wang, Tianzhen Xu, Xingxing Zhang, Cai Lin, Weiyang Gao, Huazi Xu, Bo Lei, Cong Mao
Rationale: Chronic nonhealing diabetic wound therapy and complete skin regeneration remains a critical clinical challenge. The controlled release of bioactive factors from a multifunctional hydrogel was a promising strategy to repair chronic wounds. Methods: Herein, for the first time, we developed an injectable, self-healing and antibacterial polypeptide-based FHE hydrogel (F127/OHA-EPL) with stimuli-responsive adipose-derived mesenchymal stem cells exosomes (AMSCs-exo) release for synergistically enhancing chronic wound healing and complete skin regeneration...
2019: Theranostics
Wei Zhang, Yang Wang, Yichun Kong
Purpose: In this study, we aim to investigate whether mesenchymal stem cell (MSC)-derived exosomes (MSC-Exos) could regulate hyperglycemia-induced retinal inflammation by transferring microRNA-126 (miR-126). Methods: MSC-Exos were isolated from the media of human umbilical cord-derived mesenchymal stem cells (hUCMSCs), and this isolation was followed by the transfer of miR-126. MSC-Exos or MSC-Exos overexpressing miR-126 were intravitreally injected into diabetic rats in vivo and were cocultured with high glucose-affected human retinal endothelial cells (HRECs) in vitro...
January 2, 2019: Investigative Ophthalmology & Visual Science
Zeinab Zarei Behjani, Jafar Ai, Masoud Soleimani, Amir Atashi, Behnaz Taheri, Somayeh Ebrahimi-Barough, Vahid Siavashi, Sadegh Shirian, Amir Ali Hamidieh
Sepsis and related disorders, especially acute lung injury (ALI), are the most challenging life-threatening diseases in the hospital intensive care unit. Complex pathophysiology, unbalanced immune condition, and high rate of mortality complicate the treatment of sepsis. Recently, cell therapy has been introduced as a promising option to recover the sepsis symptoms. The aim of this study was to investigate the therapeutic potential of human unrestricted somatic stem cells (USSCs) isolated from human umbilical cord blood in the mouse model of ALI...
January 9, 2019: Journal of Cellular Physiology
Marie Maumus, Yves-Marie Pers, Maxime Ruiz, Christian Jorgensen, Danièle Noël
Mesenchymal stromal or stem cells (MSCs) are multipotent adult cells that can be isolated from a variety of adult or neonatal tissues, such as bone marrow, fat tissue, placenta or umbilical cord. A therapy based on MSCs can be justified in osteoarthritis (OA) thanks to their differentiation abilities but mostly, to their paracrine and immunosuppressive properties. Possible therapeutic strategies therefore rely on the articular injection of MSCs suspensions for trophic activity or the implantation of MSCs combined with biodegradable materials for tissue engineering applications...
December 2018: Médecine Sciences: M/S
Jing Zhang, Yangyan Yi, Shuifa Yang, Yuanzheng Zhu, Xuan Hu
Objective: To explore the effects of adipose-derived stem cell released exosomes (ADSC-Exos) on the proliferation, migration, and tube-like differentiation of human umbilical vein endothelial cells (HUVECs). Methods: Adipose tissue voluntarily donated by liposuction patients was obtained. The ADSCs were harvested by enzyme digestion and identified by flow cytometry and adipogenic induction. The ADSC-Exos were extracted from the supernatant of the 3rd generation ADSCs and the morphology was observed by transmission electron microscopy...
October 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
Jose Matas, Mario Orrego, Diego Amenabar, Catalina Infante, Rafael Tapia-Limonchi, Maria Ignacia Cadiz, Francisca Alcayaga-Miranda, Paz L González, Emilio Muse, Maroun Khoury, Fernando E Figueroa, Francisco Espinoza
Knee osteoarthritis (OA) is a leading cause of pain and disability. Although conventional treatments show modest benefits, pilot and phase I/II trials with bone marrow (BM) and adipose-derived (AD) mesenchymal stromal cells (MSCs) point to the feasibility, safety, and occurrence of clinical and structural improvement in focal or diffuse disease. This study aimed to assess the safety and efficacy of the intra-articular injection of single or repeated umbilical cord-derived (UC) MSCs in knee OA. UC-MSCs were cultured in an International Organization for Standardization 9001:2015 certified Good Manufacturing Practice-type Laboratory...
December 28, 2018: Stem Cells Translational Medicine
Weiwei Hu, Zehua Feng, Jun Xu, Zhi Jiang, Meijiang Feng
Alzheimer's disease (AD) is an age-related neurodegenerative disease and the most common type of dementia. Although it is still incurable, stem cell replacement therapy provides new hope for AD. Human umbilical cord mesenchymal stem cells (hUC-MSCs) have multiple differentiation potentials, which can differentiate into cholinergic-like neurons and promote the release of acetylcholine. Brain-derived neurotrophic factor (BDNF) can also promote neurogenesis and synaptic formation, reduce oxidative stress and cell death...
December 23, 2018: Brain Research
Quan-Quan Wang, Xiao-Ma Jing, Yan-Zhen Bi, Xiao-Fu Cao, Yu-Zhong Wang, Yan-Xin Li, Bao-Jun Qiao, Yun Chen, Yan-Lei Hao, Jing Hu
BACKGROUND Since the use of human umbilical cord Wharton's Jelly derived mesenchymal stromal cells (hWJ-MSCs) to treat sarcopenia has not been explored, we studied the effects of hWJ-MSCs in aged male C57BL/6J mice with sarcopenia induced by hindlimb suspension, and explored the potential mechanism. MATERIAL AND METHODS Hindlimb suspension was used to induce sarcopenia in 24-month-old C57BL/6J mice and green fluorescent protein-tagged hWJ-MSCs and controls were transplanted into mice via tail vein or local intramuscular injection...
December 20, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Negar Varaa, Saeed Azandeh, Layasadat Khorsandi, Darioush Bijan Nejad, Vahid Bayati, Amin Bahreini
Objectives: In this study, effects of encapsulated umbilical cord stem cells (UCSCs)-derived hepatocyte-like cells (HLCs) in high mannuronic alginate scaffolds was investigated on CCl4 -induced acute liver failure (ALF) in rats. Material and Methods: UCSCs were encapsulated in high mannuronic alginate scaffolds. Then the UCSCs differentiated into HLCs for treatment of CCl4 -induced ALF in rats. Thirty rats randomly divided into 5 groups: Intoxicated group received only CCl4 to induce ALF...
September 2018: Iranian Journal of Basic Medical Sciences
Xin Gu, Shi Hua, Matz Ethan, Liren Zhong, Ting Long, Cara Clouse, Wei Li, Dong Chen, HyunChul Chung, Sean Murphy, James Yoo, Guiting Lin, Tom Lu, Anthony Atala, Jackson John, Yuanyuan Zhang
OBJECTIVE: To determine the long-term therapeutic effect among three types of human cell types on erectile function recovery in a rodent model of dual neurovascular injured erectile dysfunction (NVED). MATERIALS AND METHODS: A dual NVED model was established in athymic rats by crushing the bilateral cavernous nerves and ligations of the bilateral internal pudendal nerve-vessel bundles. At the time of defect creation, three different types of human cell populations (2...
November 30, 2018: BJU International
Yahong Chen, Ke Xue, Xiaodie Zhang, Zhiwei Zheng, Kai Liu
BACKGROUND: Developing cartilage constructed with the appropriate matrix composition and persistent chondrogenesis remains an enduring challenge in cartilage defects. Cartilage progenitor cell (CPC)-based tissue engineering has attracted recent attention because of its strong chondrogenic differentiation capacity. However, due to the lack of a suitable chondrogenic niche, the clinical application of CPC-regenerated cartilage in the subcutaneous environment remains a challenge. In this study, exosomes derived from chondrocytes (CC-Exos) were used to provide the CPC constructs with a cartilage signal in subcutaneous environments for efficient ectopic cartilage regeneration...
November 21, 2018: Stem Cell Research & Therapy
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