keyword
https://read.qxmd.com/read/38537150/prediction-of-occult-hemorrhage-in-the-lower-body-negative-pressure-model-initial-validation-of-machine-learning-approaches
#1
JOURNAL ARTICLE
Navid Rashedi, Yifei Sun, Vikrant Vaze, Parikshit Shah, Ryan Halter, Jonathan T Elliott, Norman A Paradis
INTRODUCTION: Detection of occult hemorrhage (OH) before progression to clinically apparent changes in vital signs remains an important clinical problem in managing trauma patients. The resource-intensiveness associated with continuous clinical patient monitoring and rescue from frank shock makes accurate early detection and prediction with noninvasive measurement technology a desirable innovation. Despite significant efforts directed toward the development of innovative noninvasive diagnostics, the implementation and performance of the newest bedside technologies remain inadequate...
March 27, 2024: Military Medicine
https://read.qxmd.com/read/38537148/conformation-selection-by-atp-competitive-inhibitors-and-allosteric-communication-in-erk2
#2
JOURNAL ARTICLE
Jake W Anderson, David Vaisar, David N Jones, Laurel M Pegram, Guy P Vigers, Huifen Chen, John G Moffat, Natalie G Ahn
Activation of the extracellular signal-regulated kinase-2 (ERK2) by phosphorylation has been shown to involve changes in protein dynamics, as determined by hydrogen-deuterium exchange mass spectrometry (HDX-MS) and NMR relaxation dispersion measurements. These can be described by a global exchange between two conformational states of the active kinase, named 'L' and 'R,' where R is associated with a catalytically productive ATP-binding mode. An ATP-competitive ERK1/2 inhibitor, Vertex-11e, has properties of conformation selection for the R-state, revealing movements of the activation loop that are allosterically coupled to the kinase active site...
March 27, 2024: ELife
https://read.qxmd.com/read/38537080/contributions-of-cerebral-white-matter-hyperintensities-age-and-pedal-perception-to-postural-sway-in-people-living-with-hiv
#3
JOURNAL ARTICLE
Edith V Sullivan, Natalie M Zahr, Qingyu Zhao, Kilian M Pohl, Stephanie A Sassoon, Adolf Pfefferbaum
OBJECTIVE: With aging, people living with HIV (PLWH) have diminishing postural stability that increases liability for falls. Factors and neuromechanisms contributing to instability are incompletely known. Brain white matter abnormalities seen as hyperintense (WMH) signals have been considered to underlie instability in normal aging and PLWH. We questioned whether sway-WMH relations endured after accounting for potentially relevant demographic, physiological, and HIV-related variables...
March 27, 2024: AIDS
https://read.qxmd.com/read/38537022/influence-of-genetic-polymorphisms-on-oral-health-related-quality-of-life-after-root-canal-treatment
#4
JOURNAL ARTICLE
Graciane E R Queiroz, Thuanny Castilho, Ludmila Silva Guimarães, Vania Gomes Moraes, Erlange Andrade Borges da Silva, Erika Calvano Küchler, Alice Corrêa Silva-Sousa, Manoel Damião Sousa-Neto, Lívia Azeredo Alves Antunes, Leonardo Santos Antunes
UNLABELLED: To evaluate the impact of genetic polymorphisms in interleukins (IL1A rs17561, rs1304037; IL10 rs1800871; IL1RN rs9005), nitric oxide (NOS2 rs2779249, rs2897518) and suppressor of cytokine signaling (SOCS1 rs243327, rs33977706) on oral health-related quality of life (OHRQoL) of patients under-going root canal treatment (RCT). METHODS: The sample consisted of 108 participants, presenting single-rooted teeth with asymptomatic periapical periodontitis. The impact of the OHRQoL was recorded using the Oral Health Impact Profile (OHIP-14) before, seven, and 30 days after RCT...
2024: Brazilian Dental Journal
https://read.qxmd.com/read/38536963/affinity-tagged-smad1-and-smad5-mouse-lines-reveal-transcriptional-reprogramming-mechanisms-during-early-pregnancy
#5
JOURNAL ARTICLE
Zian Liao, Suni Tang, Kaori Nozawa, Keisuke Shimada, Masahito Ikawa, Diana Monsivais, Martin Matzuk
Endometrial decidualization, a prerequisite for successful pregnancies, relies on transcriptional reprogramming driven by progesterone receptor (PR) and bone morphogenetic protein (BMP)-SMAD1/SMAD5 signaling pathways. Despite their critical roles in early pregnancy, how these pathways intersect in reprogramming the endometrium into a receptive state remains unclear. To define how SMAD1 and/or SMAD5 integrate BMP signaling in the uterus during early pregnancy, we generated two novel transgenic mouse lines with affinity tags inserted into the endogenous SMAD1 and SMAD5 loci ( Smad1HA/HA and Smad5PA/PA )...
March 27, 2024: ELife
https://read.qxmd.com/read/38536934/attenuation-of-fibroblast-activation-and-fibrosis-by-adropin-in-systemic-sclerosis
#6
JOURNAL ARTICLE
Minrui Liang, Nicholas Dickel, Andrea-Hermina Györfi, Bilgesu SafakTümerdem, Yi-Nan Li, Aleix Rius Rigau, Chunguang Liang, Xuezhi Hong, Lichong Shen, Alexandru-Emil Matei, Thuong Trinh-Minh, Cuong Tran-Manh, Xiang Zhou, Ariella Zehender, Alexander Kreuter, Hejian Zou, Georg Schett, Meik Kunz, Jörg H W Distler
Fibrotic diseases impose a major socioeconomic challenge on modern societies and have limited treatment options. Adropin, a peptide hormone encoded by the energy homeostasis-associated ( ENHO ) gene, is implicated in metabolism and vascular homeostasis, but its role in the pathogenesis of fibrosis remains enigmatic. Here, we used machine learning approaches in combination with functional in vitro and in vivo experiments to characterize adropin as a potential regulator involved in fibroblast activation and tissue fibrosis in systemic sclerosis (SSc)...
March 27, 2024: Science Translational Medicine
https://read.qxmd.com/read/38536916/a-different-interpretation-of-the-diana-fmri-signal
#7
JOURNAL ARTICLE
Valerie Doan Phi Van, Sajal Sen, Alan Jasanoff
Direct detection of neural activity by functional magnetic resonance imaging (fMRI) has been a longstanding goal in neuroscience. A recent study argued that it is possible to detect neuroelectrical potentials using a specialized fMRI scanning approach the authors termed "direct imaging of neuronal activity" (DIANA). We implemented DIANA in anesthetized rats and measured responses to somatosensory stimulation, reproducing core findings of the original study. We show, however, that neural activity is neither sufficient nor necessary to produce such results...
March 29, 2024: Science Advances
https://read.qxmd.com/read/38536914/egfr-targeting-phostacs-as-a-dual-inhibitory-approach-reveals-differential-downstream-signaling
#8
JOURNAL ARTICLE
Zhenyi Hu, Po-Han Chen, Wenxue Li, Mackenzie Krone, Sijin Zheng, Jacques Saarbach, Ines Urquizo Velasco, John Hines, Yansheng Liu, Craig M Crews
We recently developed a heterobifunctional approach [phosphorylation targeting chimeras (PhosTACs)] to achieve the targeted protein dephosphorylation (TPDephos). Here, we envisioned combining the inhibitory effects of receptor tyrosine kinase inhibitors (RTKIs) and the active dephosphorylation by phosphatases to achieve dual inhibition of kinases. We report an example of tyrosine phosphatase-based TPDephos and the effective epidermal growth factor receptor (EGFR) tyrosine dephosphorylation. We also used phosphoproteomic approaches to study the signaling transductions affected by PhosTAC-related molecules at the proteome-wide level...
March 29, 2024: Science Advances
https://read.qxmd.com/read/38536913/cardiac-tissue-model-of-immune-induced-dysfunction-reveals-the-role-of-free-mitochondrial-dna-and-the-therapeutic-effects-of-exosomes
#9
JOURNAL ARTICLE
Rick Xing Ze Lu, Naimeh Rafatian, Yimu Zhao, Karl T Wagner, Erika L Beroncal, Bo Li, Carol Lee, Jingan Chen, Eryn Churcher, Daniel Vosoughi, Chuan Liu, Ying Wang, Andrew Baker, Uriel Trahtemberg, Bowen Li, Agostino Pierro, Ana C Andreazza, Claudia C Dos Santos, Milica Radisic
Despite tremendous progress in the development of mature heart-on-a-chip models, human cell-based models of myocardial inflammation are lacking. Here, we bioengineered a vascularized heart-on-a-chip with circulating immune cells to model severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)-induced acute myocarditis. We observed hallmarks of coronavirus disease (COVID-19)-induced myocardial inflammation, as the presence of immune cells augmented the secretion of proinflammatory cytokines, triggered progressive impairment of contractile function, and altered intracellular calcium transients...
March 29, 2024: Science Advances
https://read.qxmd.com/read/38536911/select-ezh2-inhibitors-enhance-viral-mimicry-effects-of-dnmt-inhibition-through-a-mechanism-involving-nfat-ap-1-signaling
#10
JOURNAL ARTICLE
Alison A Chomiak, Rochelle L Tiedemann, Yanqing Liu, Xiangqian Kong, Ying Cui, Ashley K Wiseman, Kate E Thurlow, Evan M Cornett, Michael J Topper, Stephen B Baylin, Scott B Rothbart
DNA methyltransferase inhibitor (DNMTi) efficacy in solid tumors is limited. Colon cancer cells exposed to DNMTi accumulate lysine-27 trimethylation on histone H3 (H3K27me3). We propose this Enhancer of Zeste Homolog 2 (EZH2)-dependent repressive modification limits DNMTi efficacy. Here, we show that low-dose DNMTi treatment sensitizes colon cancer cells to select EZH2 inhibitors (EZH2is). Integrative epigenomic analysis reveals that DNMTi-induced H3K27me3 accumulates at genomic regions poised with EZH2. Notably, combined EZH2i and DNMTi alters the epigenomic landscape to transcriptionally up-regulate the calcium-induced nuclear factor of activated T cells (NFAT):activating protein 1 (AP-1) signaling pathway...
March 29, 2024: Science Advances
https://read.qxmd.com/read/38536899/multi-omics-and-single-cell-omics-new-tools-in-drug-target-discovery
#11
JOURNAL ARTICLE
Joseph Loscalzo
No abstract text is available yet for this article.
April 2024: Arteriosclerosis, Thrombosis, and Vascular Biology
https://read.qxmd.com/read/38536891/composition-optical-resonances-and-doping-of-inp-ingap-nanowires-for-tandem-solar-cells-a-micro-raman-analysis
#12
JOURNAL ARTICLE
Irene Mediavilla, Jose Luis Pura, Vanessa Giselle Hinojosa, Beatriz Galiana, Lukas Hrachowina, Magnus T Borgström, Juan Jimenez
We present a micro-Raman study of InP/InGaP tandem junction photovoltaic nanowires. These nanowires render possible InGaP compositions that cannot be made in thin films due to strain. The micro-Raman spectra acquired along the nanowires reveal the existence of compositional changes in the InGaP alloy associated with the doping sequence. The heavily Zn-doped In x Ga1- x P ( x is the In molar fraction) side of the tunnel diode is Ga rich, x = 0.25, with respect to the n-type and intrinsic segments of the top cell, which are close to the nominal composition of the NWs ( x = 0...
March 27, 2024: ACS Nano
https://read.qxmd.com/read/38536871/species-specific-responses-during-seoul-orthohantavirus-infection-in-human-and-rat-lung-microvascular-endothelial-cells
#13
JOURNAL ARTICLE
Danny Noack, Mirjam C G N van den Hout, Carmen W E Embregts, Wilfred F J van IJcken, Marion P G Koopmans, Barry Rockx
Seoul orthohantavirus (SEOV) is a rat-borne zoonotic virus that is transmitted via inhalation of aerosolized infectious excreta, and can cause hemorrhagic fever with renal syndrome (HFRS) in humans worldwide. In rats, SEOV predominantly exists as a persistent infection in the absence of overt clinical signs. Lack of disease in rats is attributed to downregulation of pro-inflammatory and upregulation of regulatory host responses. As lung microvascular endothelial cells (LMECs) represent a primary target of infection in both human and rats, infections in these cells provide a unique opportunity to study the central role of LMECs in the dichotomy between pathogenicity in both species...
March 27, 2024: PLoS Neglected Tropical Diseases
https://read.qxmd.com/read/38536854/anti-inflammatory-potential-via-the-mapk-signaling-pathway-of-lactobacillus-spp-isolated-from-canine-feces
#14
JOURNAL ARTICLE
Mi Ae Park, Mirieom Park, Hyun-Jun Jang, Sung Ho Lee, Yeong Min Hwang, Soyeon Park, Donghyun Shin, Yangseon Kim
Two probiotic candidates, Lactobacillus reuteri C1 (C1) and Lactobacillus acidophilus C5 (C5), which were previously isolated from canines, were evaluated in the present study. L. reuteri and L. acidophilus have anti-oxidant, anti-inflammatory, immune-enhancing, and anti-cancer properties and exhibit various probiotic effects in humans and animals. The strains C1 and C5 demonstrated good tolerance to acid and bile salt exposure, exhibited effective adhesion to HT-29 cell monolayer, and displayed sensitivity to antibiotics, thus affirming their probiotic characteristics...
2024: PloS One
https://read.qxmd.com/read/38536818/distinct-subpopulations-of-ventral-pallidal-cholinergic-projection-neurons-encode-valence-of-olfactory-stimuli
#15
JOURNAL ARTICLE
Ronald Kim, Mala R Ananth, Niraj S Desai, Lorna W Role, David A Talmage
To better understand the function of cholinergic projection neurons in the ventral pallidum (VP), we examined behavioral responses to appetitive (APP) and aversive (AV) odors that elicited approach or avoidance, respectively. Exposure to each odor increased cFos expression and calcium signaling in VP cholinergic neurons. Activity and Cre-dependent viral vectors selectively labeled VP cholinergic neurons that were activated and reactivated in response to either APP or AV odors, but not both, identifying two non-overlapping populations of VP cholinergic neurons differentially activated by the valence of olfactory stimuli...
March 25, 2024: Cell Reports
https://read.qxmd.com/read/38536816/iqgap1-and-nwasp-promote-human-cancer-cell-dissemination-and-metastasis-by-regulating-%C3%AE-1-integrin-via-fak-and-mrtf-srf
#16
JOURNAL ARTICLE
Camilla Cerutti, Serena Lucotti, Sofia T Menendez, Nicolas Reymond, Ritu Garg, Ignacio A Romero, Ruth Muschel, Anne J Ridley
Attachment of circulating tumor cells to the endothelial cells (ECs) lining blood vessels is a critical step in cancer metastatic colonization, which leads to metastatic outgrowth. Breast and prostate cancers are common malignancies in women and men, respectively. Here, we observe that β1-integrin is required for human prostate and breast cancer cell adhesion to ECs under shear-stress conditions in vitro and to lung blood vessel ECs in vivo. We identify IQGAP1 and neural Wiskott-Aldrich syndrome protein (NWASP) as regulators of β1-integrin transcription and protein expression in prostate and breast cancer cells...
March 26, 2024: Cell Reports
https://read.qxmd.com/read/38536815/osbp-mediated-pi-4-p-cholesterol-exchange-at-endoplasmic-reticulum-secretory-granule-contact-sites-controls-insulin-secretion
#17
JOURNAL ARTICLE
Styliani Panagiotou, Kia Wee Tan, Phuoc My Nguyen, Andreas Müller, Affiong Ika Oqua, Alejandra Tomas, Anna Wendt, Lena Eliasson, Anders Tengholm, Michele Solimena, Olof Idevall-Hagren
Insulin is packaged into secretory granules that depart the Golgi and undergo a maturation process that involves changes in the protein and lipid composition of the granules. Here, we show that insulin secretory granules form physical contacts with the endoplasmic reticulum and that the lipid exchange protein oxysterol-binding protein (OSBP) is recruited to these sites in a Ca2+ -dependent manner. OSBP binding to insulin granules is positively regulated by phosphatidylinositol-4 (PI4)-kinases and negatively regulated by the PI4 phosphate (PI(4)P) phosphatase Sac2...
March 26, 2024: Cell Reports
https://read.qxmd.com/read/38536803/electromyographic-activity-of-pelvic-floor-muscles-in-different-positions-during-the-use-an-innovative-vaginal-educator-cross-sectional-study
#18
JOURNAL ARTICLE
Natália de Souza Duarte, Yury Souza De Azevedo, Emilly Cássia Soares Furtado, Lorena Jarid Freire De Araújo, Rayanne Mesquita Bendelack, Cibele Nazaré Câmara Rodrigues, Nazete Dos Santos Araujo, Pablo Fabiano Moura das Neves, Ana Clara Nunes Soares, Rayana Carvalho Barros, Tainah Lacerda Santos, Erica Feio Carneiro Nunes, Elizabeth Alves Gonçalves Ferreira, Bianca Callegari, João Simão de Melo-Neto
The pelvic floor requires an integrated anatomical structure owing to its multiple functions. Therefore, it is necessary to study methods for improving muscle recruitment during training. This study aimed to analyze the effect of using an innovative vaginal trainer on the bioelectrical activity of the pelvic floor muscles. Pelvic positioning and interference factors, such as age, childbirth, sexual activity, urinary incontinence, and menopause, were also analyzed. A cross-sectional study assessed 30 women using an evaluation form, International Consultation on Incontinence Questionnaire-Short Form, and surface electromyography...
2024: PloS One
https://read.qxmd.com/read/38536788/a-dual-computational-and-experimental-strategy-to-enhance-tslp-antibody-affinity-for-improved-asthma-treatment
#19
JOURNAL ARTICLE
Yitong Lv, He Gong, Xuechao Liu, Jia Hao, Lei Xu, Zhiwei Sun, Changyuan Yu, Lida Xu
Thymic stromal lymphopoietin is a key cytokine involved in the pathogenesis of asthma and other allergic diseases. Targeting TSLP and its signaling pathways is increasingly recognized as an effective strategy for asthma treatment. This study focused on enhancing the affinity of the T6 antibody, which specifically targets TSLP, by integrating computational and experimental methods. The initial affinity of the T6 antibody for TSLP was lower than the benchmark antibody AMG157. To improve this, we utilized alanine scanning, molecular docking, and computational tools including mCSM-PPI2 and GEO-PPI to identify critical amino acid residues for site-directed mutagenesis...
March 2024: PLoS Computational Biology
https://read.qxmd.com/read/38536783/autophagosome-biogenesis-and-organelle-homeostasis-in-plant-cells
#20
JOURNAL ARTICLE
Xiaohong Zhuang, Baiying Li, Liwen Jiang
Autophagy is one of the major highly inducible degradation processes in response to plant developmental and environmental signals. In response to different stimuli, cellular materials, including proteins and organelles, can be sequestered into a double membrane autophagosome structure either selectively or non-selectively. The formation of an autophagosome as well as its delivery into the vacuole involves complex and dynamic membrane processes. The identification and characterization of the conserved autophagy-related (ATG) proteins and their related regulators have greatly advanced our understanding of the molecular mechanism underlying autophagosome biogenesis and function in plant cells...
March 27, 2024: Plant Cell
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