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Dermal AND (adipose OR Adipocyte OR Fat)

Weiqiang Duan, Chang Cao, Junliang Wu, Ying Cen, Xuewen Xu, Yong Liu
Objective: To explore the effectiveness of modified inferior pedicle technique with inverted T pattern for severe breast hypertrophy. Methods: Between January 2016 and May 2017, 15 patients of severe breast hypertrophy had undergone breast reduction using inferior pedicle technique with inverted T pattern combined with dermal suspension sling technique. The patients were 20 to 49 years old, with an average age of 31.6 years. Body mass index ranged from 24.9 to 32...
March 1, 2019: Chinese Journal of Reparative and Reconstructive Surgery
Robert P Gersch, Jeffrey C Raum, Catherine E Calvert, Ivona Percec
BACKGROUND: The efficacy of adipose-derived stem cells (ASCs) to improve wound healing has been extensively investigated. Unfortunately, there have been no consistent reports describing significant improvements in healing time or outcomes in large-scale clinical trials using human ASCs. Primarily, these studies examined undifferentiated ASCs as opposed to specific cells differentiated from ASCs. OBJECTIVE: Here, we examine the properties of fibroblasts differentiated from human ASCs (dFib cells) in comparison to those of primary dermal fibroblasts...
March 15, 2019: Aesthetic Surgery Journal
Gloria R Sue, Oscar H Ho
BACKGROUND: Facial paralysis is a significant problem with functional, psychological, and esthetic consequences. Free muscle transfer for reanimation of the smile has been established as the preferred reconstructive method. However, little has been reported on the complications after this procedure. We sought to perform a critical analysis of these complications and their ultimate outcomes. METHODS: A retrospective review was performed on consecutive patients undergoing microsurgical reconstruction of the smile by the senior author from 2013 through 2017...
March 12, 2019: Annals of Plastic Surgery
Hui Zheng, Lihong Qiu, Yingjun Su, Chenggang Yi
BACKGROUND: Nanofats could improve photoaging. Stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) may play pivotal roles. However, SVFs and ADSCs in nanofats processed by conventional methods cannot be enriched. Some researchers have found that after centrifugation, the SVF/ADSC density increases from top to bottom. OBJECTIVES: We hypothesized that centrifugation can be used to obtain SVF/ADSC-concentrated nanofats that are superior to conventional nanofats in improving the photoaging of skin...
March 14, 2019: Aesthetic Surgery Journal
Philip R Cohen, Christopher P Erickson, Antoanella Calame
Painful tumors of the skin present as dermal or subcutaneous nodules. They can originate from several sources: adipose tissue, cartilage degeneration, deposition of bone or calcium, eccrine glands, fibrous tissue, infiltration of benign (endometrium) or malignant (metastatic neoplasm) tissue, muscle, nerve, or vascular structures. Although pathologic evaluation of the lesion is necessary to determine the diagnosis, it is possible to make a reasonable differential diagnosis based on knowledge of prior tumors that have appeared as tender lesions...
2019: Clinical, Cosmetic and Investigational Dermatology
Hiroshi Nakagomi, Masayuki Inoue, Haruka Nakada, Masato Ohmori, Yuko Nakayama, Kazushige Furuya, Hideki Watanabe, Kou Ikegame, Koichi Kobayashi
BACKGROUND: Oncoplastic breast conserving surgery had been challenged to achieve both of local control and the cosmetic appearance of preserved breast. We developed the lateral thoracoaxillar dermal-fat flap (LTDF) as an oncoplastic procedure to fill the defect of breast-conserving surgery in 1999. METHODS: A total of 2338 breast cancer patients underwent surgery from January, 2000 to December, 2017. Mastectomy was performed in 706 patients (30%), and breast conservative surgery (BCS) was performed in 1634 patients (70%)...
March 7, 2019: Breast Cancer: the Journal of the Japanese Breast Cancer Society
Rachel Lentz, Andre Alcon, Hani Sbitany
Background: Breast animation deformity is a known complication associated with submuscular prosthetic breast reconstruction. Patients often will present months to years after their initial reconstruction complaining of chronic pain and visible contraction deformity of their chest, with minimal voluntary activation of their pectoralis musculature. This is aesthetically displeasing and physically uncomfortable. Our preferred method for addressing existing animation deformity and alleviating patients' symptoms involves reoperation, with implant pocket conversion to the prepectoral plane, with acellular dermal matrix (ADM) coverage...
February 2019: Gland Surgery
Glyn Jones, Anuja K Antony
Background: Given the current trends in skin preservation during mastectomy, improved biofilm reduction algorithms, and advancements in tissue bioengineering and perfusion assessment, acellular dermal matrix (ADM)-reinforced single stage, direct to implant insertion in the pre-pectoral space has become a viable alternative to two-stage expander-based, sub-pectoral reconstruction. Methods: The authors performed a retrospective review of their pre-pectoral cases evaluating outcomes for all single stage, direct to implant procedures...
February 2019: Gland Surgery
Adrian Djalali-Cuevas, Sergio Garnica-Galvez, Andrea Rampin, Diana Gaspar, Ioannis Skoufos, Athina Tzora, Nikitas Prassinos, Nikolaos Diakakis, Dimitrios I Zeugolis
Tissue engineering by self-assembly allows for the fabrication of living tissue surrogates by taking advantage of the cell's inherent ability to produce and deposit tissue-specific extracellular matrix. However, the long culture periods required to build a tissue substitute in conducive to phenotypic drift in vitro microenvironments result in phenotype and function losses. Although several biophysical microenvironmental modulators (e.g., surface topography, substrate stiffness, mechanical stimulation) have been used to address these issues, slow extracellular matrix deposition remains a limiting factor in clinical translation and commercialization of such therapies...
2019: Methods in Molecular Biology
Xi Zhang, Lin Chen, Bo Xiao, Hengxin Liu, Yingjun Su
It remains unclear why obese persons displayed a slower wound healing rate than the normal. In this study, we found that has_circ_0075932, a single-exon circular RNA, was outstandingly expressed in human normal adipose tissue and overexpressed in burned skin of obese persons compared with that of non-obese persons. Circ_0075932 overexpression or silencing in dermal keratinocytes had no obvious effect on cell behaviors, unless dozens of times overexpression, since its basal expression level in keratinocytes is too low...
February 26, 2019: Biochemical and Biophysical Research Communications
Kazutaka Soejima, Tsutomu Kashimura, Tomohiko Kazama, Taro Matsumoto, Hiroaki Nakazawa
BACKGROUND: Artificial dermis (AD) is an important option for preparing full-thickness wounds for cultured epithelial autograft (CEA). Long-term fragility after CEA remains a problem, probably due to lack of basement membrane (BM) proteins. We hypothesized that treating AD with mesenchymal stem cells would promote BM protein production. We tested this using dedifferentiated fat (DFAT) cells in a porcine experimental model. METHODS: This study employed four male LWD swine...
February 14, 2019: Plastic and Reconstructive Surgery
Carina Nicu, Jonathan Hardman, Jennifer Pople, Ralf Paus
In murine skin dermal white adipose tissue (DWAT) undergoes fluctuations in size across the hair cycle, whereas changes in size, function and metabolism of dermal adipocytes (DAs) during the human scalp hair cycle remain unexplored. Transmission electron microscopy results suggest that, during anagen-catagen transition, human DAs co-opt the autophagy machinery to undergo lipophagy within their lipid droplets. Wholemount staining of hair follicles (HFs) and surrounding DWAT for the autophagy marker LC3B confirms the increased presence of LC3B+ lipid droplets adjacent to catagen HFs; moreover, DWAT around catagen HFs engages in greater glycerol release compared to DWAT surrounding anagen HFs...
February 18, 2019: Experimental Dermatology
Helena Debiazi Zomer, Gisele Kristina Dos Santos Varela, Priscilla Barros Delben, Diana Heck, Talita da Silva Jeremias, Andrea Gonçalves Trentin
Novel strategies combining cell therapy, tissue engineering and regenerative medicine have been developed to treat major skin wounds. Although mesenchymal stromal cells (MSCs) from different tissues have similar stem cell features, such as self-renewing mesodermal differentiation potential and expression of immunophenotypic markers, they also have distinct characteristics. Therefore, we aimed to characterize the application of MSCs derived from the dermis and adipose tissue (DSCs and ASCs, respectively) in cutaneous wound healing by in vitro approaches...
February 18, 2019: Journal of Tissue Engineering and Regenerative Medicine
Yasuhiko Kaita, Takehiko Tarui, Hideaki Yoshino, Takeaki Matsuda, Yoshihiro Yamaguchi, Takatoshi Nakagawa, Michio Asahi, Masaaki Ii
Introduction: The purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs. Methods: Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses...
June 2019: Regenerative Therapy
Atefeh Pesaraklou, Nasser Mahdavi-Shahri, Halimeh Hassanzadeh, Masoud Ghasemi, Mahboubeh Kazemi, Nasser Sanjar Mousavi, Maryam M Matin
Today advancements in nanotechnology have made extensive progress in tissue engineering. Application of cerium oxide nanoparticles (CeO2) has improved regenerative medicine due to their antioxidant properties. In this study, nanoparticles were used to increase the efficacy of skin substitutes. Human skin samples were decellularized using four methods and studied via histological stainings and DNA content analyses. Then CeO2 dispersing and its stability were investigated. The prepared acellular dermal matrices (ADMs) were immersed in CeO2 suspension and their effects were evaluated on growth of cultured human adipose derived-mesenchymal stem cells (hAd-MSCs) using MTT assay and histological methods...
February 12, 2019: Biomedical Materials
Lin-Gwei Wei, Hsin-I Chang, Yiwei Wang, Shan-Hui Hsu, Lien-Guo Dai, Keng-Yen Fu, Niann-Tzyy Dai
Background: A tissue-engineered skin substitute, based on gelatin ("G"), collagen ("C"), and poly(ε-caprolactone) (PCL; "P"), was developed. Method: G/C/P biocomposites were fabricated by impregnation of lyophilized gelatin/collagen (GC) mats with PCL solutions, followed by solvent evaporation. Two different GC:PCL ratios (1:8 and 1:20) were used. Results: Differential scanning calorimetry revealed that all G/C/P biocomposites had characteristic melting point of PCL at around 60 °C...
2019: PeerJ
Ji Suk Choi, Woo Lee Cho, Yeo Jin Choi, Jae Dong Kim, Hyun-A Park, Su Yeon Kim, Jae Hyung Park, Dong-Gyu Jo, Yong Woo Cho
Ultraviolet-B (UVB) irradiation causes imbalance between dermal matrix synthesis and degradation through aberrant upregulation of matrix metalloproteinases (MMPs), which leads to overall skin photoaging. We investigated the effects of extracellular vesicles (EVs) derived from human adipose-derived stem cells (HASCs) on photo-damaged human dermal fibroblasts (HDFs). EVs were isolated from conditioned media of HASCs with tangential flow filtration and characterized using transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA), western blotting, micro RNA (miRNA) arrays, cytokine arrays and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS)...
2019: Journal of Extracellular Vesicles
Hira Raheel, Siavash Ghaffari, Negar Khosraviani, Victoria Mintsopoulos, Derek Auyeung, Changsen Wang, Yun Hye Kim, Brendan Mullen, Hoon-Ki Sung, May Ho, Gregory Fairn, Dante Neculai, Maria Febbraio, Bryan Heit, Warren L Lee
In healthy blood vessels, albumin crosses the endothelium to leave the circulation by transcytosis. However, little is known about the regulation of albumin transcytosis or how it differs in different tissues; its physiological purpose is also unclear. Using total internal reflection fluorescence microscopy, we quantified transcytosis of albumin across primary human microvascular endothelial cells from both lung and skin. We then validated our in vitro findings using a tissue-specific knockout mouse model. We observed that albumin transcytosis was saturable in the skin but not the lung microvascular endothelial cells, implicating a receptor-mediated process...
January 31, 2019: American Journal of Physiology. Lung Cellular and Molecular Physiology
Zhu Zhu, Zhaoqi Yuan, Cheng Huang, Rui Jin, Di Sun, Jun Yang, Xusong Luo
Heterologous acellular dermal matrix (ADM) has good biocompatibility and sufficient strength for clinical use for the repair of defects, tissue filling, and resurfacing of deep wounds. However, ADM tissue has such a compact structure that it can easily result in delayed vascularization after implantation. Moreover, in spite of the low immunogenicity of heterologous ADM, it can still cause varying degrees of inflammation in the host. These two drawbacks limit the efficacy and scope of clinical applications for heterologous ADM...
January 30, 2019: Biomedical Materials
Yuko Kijima, Munetsugu Hirata, Yoshiaki Shinden, Toshiaki Utsumi, Zenichi Morise, Shoji Natsugoe
Oncoplastic breast surgery (OBS), which combines the concepts of oncologic and plastic surgery, is becoming more common worldwide. We report the results of OBS in a Japanese patient with early breast cancer located on the outer lower quadrant area. We performed OBS combining partial mastectomy with immediate breast reshaping using multiple adipofascial cutaneous flaps and free dermal fat graft because she refused any other OBS. We selected three local flaps to repair the defect. Perioperative and postoperative complications were not seen...
January 25, 2019: Breast Cancer: the Journal of the Japanese Breast Cancer Society
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