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https://read.qxmd.com/read/30774379/effects-of-combined-inhibition-of-stat3-and-vegfr2-pathways-on-the-radiosensitivity-of-non-small-cell-lung-cancer-cells
#1
Chenxi Hu, Wei Zhuang, Yun Qiao, Bin Liu, Liang Liu, Kaiyuan Hui, Xiaodong Jiang
Purpose: The goals of this study were to determine the effects of combined inhibition of STAT3 and vascular endothelial growth factor receptor 2 (VEGFR2) pathways on the radiosensitivity of non-small-cell lung cancer (NSCLC) cells, and to assess the underlying mechanisms. Methods: The expressions of VEGFR2, STAT3, related signaling molecules, hypoxia-inducible factor 1-alpha (HIF-1α), and cyclin D1 were determined by Western blotting. Radiosensitivity was assessed using the colony-forming assay, and cell cycle and cell death were analyzed by flow cytometry...
2019: OncoTargets and Therapy
https://read.qxmd.com/read/30774370/the-inhibitive-effect-of-sh-hif1a-as2-on-the-proliferation-invasion-and-pathological-damage-of-breast-cancer-via-targeting-mir-548c-3p-through-regulating-hif-1%C3%AE-vegf-pathway-in-vitro-and-vivo
#2
Xiao Guo, Shenghai Lee, Peilong Cao
Background: Breast cancer (BC) has been the commonest malignant tumor with a low survival rate among woman. Long non-coding RNA hypoxia-inducible factor-1 alpha antisense RNA-2 (HIF1A-AS2) was correlated with various cancers. Purpose: The study aimed to investigate the roles and related underlying molecular mechanisms of HIF1A-AS2 in BC. Material and methods: Target relationships were speculated by Targetscan 7.0 and confirmed by dual luciferase reporter assay...
2019: OncoTargets and Therapy
https://read.qxmd.com/read/30771687/unfractionated-heparin-alleviates-sepsis-induced-acute-lung-injury-by-protecting-tight-junctions
#3
Yina Liu, Shengtian Mu, Xu Li, Yingjian Liang, Liang Wang, Xiaochun Ma
BACKGROUND: Unfractionated heparin (UFH) has been shown to ameliorate lung edema and lung vascular leakage in lipopolysaccharide-induced lung injury. Impaired tight junction (TJ) function is a sign of sepsis-induced acute respiratory distress syndrome (ARDS) and acute lung injury (ALI), which is closely related to the downregulated expression of TJ-specific proteins or the upregulated expression of inflammatory cytokines. Because UFH has been intensively studied in modulating inflammation, we hypothesize that UFH may play a positive role in treating sepsis-induced ARDS/ALI by protecting TJs...
February 13, 2019: Journal of Surgical Research
https://read.qxmd.com/read/30767433/social-defeat-stress-induces-phosphorylation-of-extracellular-signal-regulated-kinase-in-the-leptomeninges-in-mice
#4
Satoshi Okamura, Hirotaka Nagai, Chisato Numa, Midori Nagai, Ryota Shinohara, Tomoyuki Furuyashiki
AIMS: Animal studies using various stress models have shown that excessive environmental stress induces depression? and anxiety?like behaviors through inflammatory responses in the brain and periphery. Although the leptomeningeal cells have multiple functions related to inflammatory responses in the brain, whether environmental stress influences the leptomeninges remains unknown. In this study, we aimed to examine phosphorylation of the extracellular signal-regulated kinase (ERK) in the leptomeninges...
February 14, 2019: Neuropsychopharmacology reports
https://read.qxmd.com/read/30766925/pmab-219-a-monoclonal-antibody-for-the-immunohistochemical-analysis-of-horse-podoplanin
#5
Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Masato Sano, Hiroyuki Harada, Masato Fukui, Mika K Kaneko, Yukinari Kato
Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG2a , kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting...
July 2019: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/30762134/tethering-qk-peptide-to-enhance-angiogenesis-in-elastin-like-recombinamer-elr-hydrogels
#6
Tatjana Flora, I González de Torre, M Alonso, J Carlos Rodríguez-Cabello
The development of new capillary networks in engineered constructs is essential for their survival and their integration with the host tissue. It has recently been demonstrated that ELR-based hydrogels encoding different bioactivities are able to modulate their interaction with the host after injection or implantation, as indicated by an increase in cell adhesion and the ability to trigger vascularization processes. Accordingly, the aim of this study was to increase their angiogenic ability both in vitro and in vivo using a small VEGF mimetic peptide named QK, which was tethered chemically to ELR-based hydrogels containing cell-adhesion sequences in their backbone, such as REDV and RGD, as well as a proteolytic site (VGVAPG)...
February 14, 2019: Journal of Materials Science. Materials in Medicine
https://read.qxmd.com/read/30761147/massive-b-cell-infiltration-and-organization-into-artery-tertiary-lymphoid-organs-in-the-aorta-of-large-vessel-giant-cell-arteritis
#7
Jacoba C Graver, Annemieke M H Boots, Erlin A Haacke, Arjan Diepstra, Elisabeth Brouwer, Maria Sandovici
Giant cell arteritis (GCA) can be classified into Cranial(C)-GCA and Large Vessel(LV)-GCA. Based on analysis of temporal arteries, GCA is postulated to be T-cell-mediated. Recently, a disturbed B-cell homeostasis was documented in newly diagnosed GCA patients. In the current study, we assessed the presence of B-cells and their level of ectopic organization in the aorta of LV-GCA patients. Aorta tissue samples of 9 histologically-proven LV-GCA patients and 22 age- and sex-matched atherosclerosis patients who underwent aortic aneurysm surgery were studied by immunohistochemistry...
2019: Frontiers in Immunology
https://read.qxmd.com/read/30760630/increase-of-glandular-epithelial-cell-clusters-by-an-external-volume-expansion-device-promotes-adipose-tissue-regeneration-by-recruiting-macrophages
#8
Xihang Chen, Yunfan He, Anqi Xu, Zilong Deng, Jinwei Feng, Feng Lu, Yi Yuan
BACKGROUND: There is a clinical need for the use of engineered adipose tissue in place of surgical reconstruction. We previously found that the external volume expansion (EVE) device increased special cell clusters in well-vascularized connective stroma during adipose regeneration. However, the origin of these cell clusters and their role in adipose tissue regeneration remains unknown. AIM: In this study, we evaluated EVE in the construction of expanded prefabricated adipose tissue (EPAT) in a rat model...
February 13, 2019: Bioscience Reports
https://read.qxmd.com/read/30756082/the-effects-of-korea-red-ginseng-on-inflammatory-cytokines-and-apoptosis-in-rat-model-with-chronic-nonbacterial-prostatitis
#9
Sang Wook Kang, Je-Hoon Park, Hosik Seok, Hae Jeong Park, Joo-Ho Chung, Chang-Ju Kim, Young Ock Kim, Young Rok Han, DongWhan Hong, Young Sik Kim, Su Kang Kim
Chronic prostatitis typically occurs in aging men, and its symptoms include frequent and painful urination. In recent study, several studies have shown that Korean red ginseng (KRG) can be used in the prevention and treatment of various diseases. The objective of this study is to investigate whether KRG can play a role in repressing the development of chronic nonbacterial prostatitis (CNP) in male Wistar rats. To induce CNP, rats were castrated and beta-estradiol (0.25 mg/kg) was subcutaneously (s.c.) injected daily...
2019: BioMed Research International
https://read.qxmd.com/read/30746892/vascular-endothelial-growth-factor-c-treatment-for-mouse-hind-limb-lymphatic-revascularization
#10
Juliana S P Ferrão, Antenor P Bonfim Neto, Vanessa U da Fonseca, Liza M M de C Sousa, Paula de C Papa
Spontaneous lymphatic revascularization is a challenge and the establishment of new therapeutic strategies may improve life quality for patients suffering from lymphatic disorders. This study was designed to verify if VEGFC treatment improves lymphatic vascularization in a time-dependent manner in mouse hindlimb (HL) after resection of the inguinal lymph node. Lymphatic vascular density (Vv) and length (Lv) were evaluated by stereology after immunohistochemistry. The control Group (CG) was not manipulated but received saline instead of VEGFC treatment...
February 11, 2019: Veterinary Medicine and Science
https://read.qxmd.com/read/30745851/fbxo22-suppresses-metastasis-in-human-renal-cell-carcinoma-via-inhibiting-mmp-9-mediated-migration-and-invasion-and-vegf-mediated-angiogenesis
#11
Feng Guo, Jinjin Liu, Xiao Han, Xuping Zhang, Tian Lin, You Wang, Jin Bai, Junqing Han
F-box only protein 22 (FBXO22), a substrate receptor of the SKP1-Cullin 1-F-box protein (SCF) E3 ubiquitin ligase that targets key regulators of cellular activities for ubiquitylation and degradation, plays important roles in the progression of human cancer. However, little is known about the role of FBXO22 in renal cell carcinoma (RCC). This study aims to explore the biological function of FBXO22 in RCC progression and its specific regulation mechanism. We performed immunohistochemistry analysis and found that the expression level of FBXO22 was significantly lower in RCC tissues than in normal renal tissues...
2019: International Journal of Biological Sciences
https://read.qxmd.com/read/30740857/the-endothelial-lectin-clearance-receptor-clec4m-binds-and-internalizes-factor-viii-in-a-vwf-dependent-and-independent-manner
#12
L L Swystun, C Notley, I Georgescu, J D Lai, K Nesbitt, P D James, D Lillicrap
BACKGROUND: von Willebrand factor (VWF) and factor VIII (FVIII) circulate in the plasma as a non-covalent complex, and the majority of FVIII is likely cleared by VWF-dependent pathways. Clearance of VWF-free FVIII is rapid and underlies the pathological basis of some quantitative FVIII deficiencies. The receptor pathways that regulate the clearance of VWF-bound and VWF-free FVIII are incompletely uncharacterized. The human liver expressed endothelial lectin CLEC4M has been previously characterized as a clearance receptor for VWF, although its influence on FVIII is unknown OBJECTIVES: The interaction between FVIII and CLEC4M was characterized in the presence or absence of VWF...
February 11, 2019: Journal of Thrombosis and Haemostasis: JTH
https://read.qxmd.com/read/30712668/histopathological-analysis-of-placentas-with-congenital-cytomegalovirus-infection
#13
Mizuki Uenaka, Mayumi Morizane, Kenji Tanimura, Masashi Deguchi, Maki Kanzawa, Tomoo Itoh, Hideto Yamada
INTRODUCTION: Cytomegalovirus (CMV) infection is the most common cause of congenital viral infections in humans. The unusual structure of the placenta plays a pivotal role in CMV transmission from mothers to fetuses. The aim of this study was to evaluate the histopathological findings of placentas with congenital CMV infections. METHODS: We obtained placental specimens from 35 women who had newborns with congenital CMV infections. Placental specimens, extraplacental membranes, and umbilical cords were stained with hematoxylin and eosin, and subjected to immunohistochemical analysis...
January 2019: Placenta
https://read.qxmd.com/read/30711173/inhibition-of-nuclear-factor-kappa-b-prevents-the-development-of-experimental-periapical-lesions
#14
Tomoatsu Kaneko, Su Yee Myo Zaw, Yukiko Sueyama, Ken-Ichi Katsube, Reika Kaneko, Jacques E Nör, Takashi Okiji
INTRODUCTION: Nuclear factor kappa B (NF-κB) is an important transcriptional regulator of angiogenesis involving B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax) signaling pathways. Thus, inhibition of NF-κB may suppress the development of periapical lesions via blockage of angiogenesis. Accordingly, we examined the effects of NF-κB decoy oligodeoxynucleotide (ODN) treatment on experimentally induced periapical lesions. METHODS: Periapical lesions were induced in the mandibular first molars of 5-week-old male Wistar rats by the application of lipopolysaccharide to the pulp...
February 2019: Journal of Endodontics
https://read.qxmd.com/read/30706534/histological-immunohistochemical-and-genomic-evaluation-of-excisional-and-diabetic-wounds-treated-with-human-wharton-s-jelly-stem-cells-with-and-without-a-nanocarrier
#15
Vaishnevi Raj, Stephanie Claudine, Arjunan Subramanian, Kimberley Tam, Arijit Biswas, Ariff Bongso, Chui-Yee Fong
We showed in previous studies that human umbilical cord Wharton's jelly stem cells (hWJSCs) improved the healing rates of excisional and diabetic wounds in the mouse model. As an extension of those studies, we report here the more detailed quantitative histological, immunohistochemical, and genomic evaluation of biopsies from those excisional and diabetic wounds in an attempt to understand the mechanisms of the enhanced wound healing aided by hWJSCs. Bright-field microscopic observations and ImageJ software analysis on histological sections of the excisional and diabetic wound biopsies collected at different time points showed that the thickness of the epidermis and dermis, and positive picrosirius-red stained areas for collagen, were significantly greater in the presence of hWJSCs compared with controls (P < 0...
January 31, 2019: Journal of Cellular Biochemistry
https://read.qxmd.com/read/30704100/human-brain-endothelial-cxcr2-is-inflammation-inducible-and-mediates-cxcl5-and-cxcl8-triggered-paraendothelial-barrier-breakdown
#16
Axel Haarmann, Michael K Schuhmann, Christine Silwedel, Camelia-Maria Monoranu, Guido Stoll, Mathias Buttmann
Chemokines (C-X-C) motif ligand (CXCL) 5 and 8 are overexpressed in patients with multiple sclerosis, where CXCL5 serum levels were shown to correlate with blood⁻brain barrier dysfunction as evidenced by gadolinium-enhanced magnetic resonance imaging. Here, we studied the potential role of CXCL5/CXCL8 receptor 2 (CXCR2) as a regulator of paraendothelial brain barrier function, using the well-characterized human cerebral microvascular endothelial cell line hCMEC/D3. Low basal CXCR2 mRNA and protein expression levels in hCMEC/D3 were found to strongly increase under inflammatory conditions...
January 30, 2019: International Journal of Molecular Sciences
https://read.qxmd.com/read/30703600/the-relationship-between-apoptosis-of-endplate-microvasculature-and-degeneration-of-the-intervertebral-disc
#17
Hong-Ming Xu, Fei Hu, Xiang-Yang Wang, Song-Lin Tong
OBJECTIVE: To explore the relationship between intervertebral disc degeneration and endplate microvasculature, and determine the role of apoptosis in the pathophysiology underlying endplate microvasculature. METHODS: Twelve 6-month-old rabbits were randomly divided into Groups A (The Control Group, animals were accepted a sham operation, in which the loading device was implanted but without loading) and B (The Degeneration Group, a calibrated spring within the loading device would immediately create static shear force of 50 N to the disc of L4/5)...
January 28, 2019: World Neurosurgery
https://read.qxmd.com/read/30695042/fc-gamma-riib-expression-levels-in-human-liver-sinusoidal-endothelial-cells-during-progression-of-non-alcoholic-fatty-liver-disease
#18
Tomoko Ishikawa, Hiroshi Yokoyama, Tomokazu Matsuura, Yoko Fujiwara
Liver sinusoidal endothelial cells (LSECs) play a pivotal role in hepatic function and homeostasis. LSEC dysfunction has been recognized to be closely involved in various liver diseases, including non-alcoholic steatohepatitis (NASH), but not much is known about the fate of the scavenger receptors in LSECs during NASH. Fc gamma receptor IIb (FcγRIIb), known as a scavenger receptor, contributes to receptor-mediated endocytosis and immune complexes clearance. In this study, to elucidate the fate of FcγRIIb in the progression of non-alcoholic fatty liver disease (NAFLD), we examined FcγRIIb levels in NAFLD biopsy specimens by immunohistochemistry, and investigated their correlation with the exacerbation of biological indexes and clinicopathological scores of NASH...
2019: PloS One
https://read.qxmd.com/read/30692122/thrombotic-microangipathy-as-a-cause-for-cardiovascular-toxicity-from-the-bcr-abl1-tyrosine-kinase-inhibitor-ponatinib
#19
Yllka Latifi, Federico Moccetti, Melinda Wu, Aris Xie, William Packwood, Yue Qi, Koya Ozawa, Weihui Shentu, Eran Brown, Toshiaki Shirai, Owen J McCarty, Zaverio Ruggeri, Javid Moslehi, Junmei Chen, Brian J Druker, Jose A López, Jonathan R Lindner
The third generation tyrosine kinase inhibitor (TKI) ponatinib is effective against drug-resistant chronic myelogenous leukemia, but has been plagued by high rates of acute ischemic events. The pathophysiology responsible for these events is unknown. We hypothesized that ponatinib produces an endothelial angiopathy involving excessive endothelial-associated von Willebrand factor (VWF) and secondary platelet adhesion. In wild-type mice and ApoE-/- mice on Western diet, contrast-enhanced ultrasound molecular imaging of the thoracic aorta for VWF A1-domain and glycoprotein-Ibα was performed to quantify endothelial-associated VWF and platelet adhesion...
January 28, 2019: Blood
https://read.qxmd.com/read/30691610/canine-cutaneous-haemangiosarcoma-biomarkers-and-survival
#20
D F Nóbrega, V F Sehaber, R Madureira, A P F R L Bracarense
Haemangiosarcoma (HSA) is a malignant tumour of vascular endothelial cells. It is common in dogs, but rare in other species, and may arise in any tissue. A dermal location of canine HSA has been associated with short survival and recurrence after surgical excision. Solar radiation has been proposed as a predisposing factor in shorthaired dogs with light skin pigmentation. There are no studies relating the expression of immunohistochemical markers to survival of dogs with cutaneous haemangiosarcoma (cHSA). Such data could contribute to establishing prognostic factors and new therapies based on the expression of target molecules...
January 2019: Journal of Comparative Pathology
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