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https://read.qxmd.com/read/30771686/fish-oil-increases-specialized-pro-resolving-lipid-mediators-in-pad-the-omega-pad-ii-trial
#1
Joel L Ramirez, Warren J Gasper, Sukaynah A Khetani, Greg J Zahner, Nancy K Hills, Pete T Mitchell, Brian E Sansbury, Michael S Conte, Matthew Spite, S Marlene Grenon
BACKGROUND: N-3 polyunsaturated fatty acid (PUFA) supplementation has been associated with reduced mortality and inflammation in patients with cardiovascular disease. There are limited data on the effects of n-3 PUFA supplementation in patients with peripheral artery disease (PAD). MATERIALS AND METHODS: The OMEGA-PAD II trial was a double-blinded, randomized, placebo-controlled trial to assess the effect of 3 mo of high-dose oral n-3 PUFA supplementation on inflammation, endothelial function, and walking ability in patients with PAD...
February 13, 2019: Journal of Surgical Research
https://read.qxmd.com/read/30771456/vegf-c-attenuates-ischemia-reperfusion-injury-of-liver-graft-in-rats
#2
Ming-Xiang Cheng, Jin-Zheng Li, Yong Chen, Ding Cao, Jian-Ping Gong, Bing Tu
BACKGROUND: Vascular endothelial growth factor receptor-3 (VEGFR-3) / vascular endothelial growth factor -c (VEGF-C) signaling is reported to negatively regulate TLR4-triggered inflammation of macrophages. This study aims to clarify whether the VEGFR-3/VEGF-C signaling can suppress Kupffer cells (KCs) activation and attenuate hepatic ischemia-reperfusion injury (IRI) after liver transplantation. METHODS: A rat model of liver transplantation was performed. Donor livers were perfused with VEGF-C injection via portal vein during cold preservation, and controls were perfused with UW solution...
February 13, 2019: Transplant Immunology
https://read.qxmd.com/read/30770921/selective-loss-of-cortical-endothelial-tight-junction-proteins-during-alzheimer-s-disease-progression
#3
Yu Yamazaki, Mitsuru Shinohara, Motoko Shinohara, Akari Yamazaki, Melissa E Murray, Amanda M Liesinger, Michael G Heckman, Elizabeth R Lesser, Joseph E Parisi, Ronald C Petersen, Dennis W Dickson, Takahisa Kanekiyo, Guojun Bu
While the accumulation and aggregation of amyloid-β and tau are central events in the pathogenesis of Alzheimer's disease, there is increasing evidence that cerebrovascular pathology is also abundant in Alzheimer's disease brains. In brain capillaries, endothelial cells are connected closely with one another through transmembrane tight junction proteins forming the blood-brain barrier. Because the blood-brain barrier tightly regulates the exchange of molecules between brain and blood and maintains brain homeostasis, its impairment is increasingly recognized as a critical factor contributing to Alzheimer's disease pathogenesis...
February 15, 2019: Brain: a Journal of Neurology
https://read.qxmd.com/read/30770282/the-effect-of-honokiol-on-pulmonary-artery-endothelium-cell-autophagy-mediated-by-cyclophilin-a-in-hypoxic-pulmonary-arterial-hypertension
#4
Xiaoying Wang, Dandan Xiao, Cui Ma, Lixin Zhang, Qingya Duan, Xiaodong Zheng, Min Mao, Daling Zhu, Qian Li
Abnormal autophagy plays critical roles in the structure and function of the pulmonary vasculature. Cyclophilin A (CyPA) can be secreted from cells in response to hypoxia and oxidative stress, which are involved in inducing autophagy and regulating the function of endothelial cells in pulmonary arterial hypertension. Honokiol is a small molecule natural compound; it has many bioactivities, such as antitumor, anti-inflammatory, antioxidant and antiangiogenic properties, but how honokiol mediates autophagy in pulmonary arterial hypertension is unclear...
February 1, 2019: Journal of Pharmacological Sciences
https://read.qxmd.com/read/30769337/bioprinted-osteon-like-scaffolds-enhance-in-vivo-neovascularization
#5
Charlotte Piard, Hannah Baker, Timur Kamalitdinov, John P Fisher
Bone tissue engineers are facing a daunting challenge when attempting to fabricate bigger constructs intended for use in the treatment of large bone defects, which is the vascularization of the graft. Cell-based approaches and, in particular, the use of in vitro coculture of human umbilical vein endothelial cells (HUVECs) and human mesenchymal stem cells (MSCs) has been one of the most explored options. We present in this paper an alternative method to mimic the spatial pattern of HUVECs and hMSCs found in native osteons based on the use of extrusion-based 3D bioprinting (3DP)...
February 15, 2019: Biofabrication
https://read.qxmd.com/read/30769250/vasoreactivity-to-acetylcholine-during-porcine-kidney-perfusion-for-the-assessment-of-ischemic-injury
#6
Michael F Bath, Sarah A Hosgood, Michael L Nicholson
BACKGROUND: The effects of renal allograft ischemic injury on vascular endothelial function have not been clearly established. The aim of this study was to examine vascular reactivity to acetylcholine (ACh) in kidneys subjected to ischemic injury and reperfusion. METHODS: Porcine kidneys were exposed to different combinations of warm ischemic time (WIT) and cold ischemic time (CIT) as follows: 15 min (n = 7), 60 min (n = 6), 90 min (n = 6), or 120 min (n = 4) WIT + 2 h CIT or 15 min WIT + 16 h CIT (n = 8)...
February 12, 2019: Journal of Surgical Research
https://read.qxmd.com/read/30769049/aloin-reduces-inflammatory-gene-inos-via-inhibition-activity-and-p-stat-1-and-nf-%C3%AE%C2%BAb
#7
Wonhwa Lee, Sumin Yang, Changhun Lee, Eui Kyun Park, Kyung-Min Kim, Sae-Kwang Ku, Jong-Sup Bae
Aloin is the major anthraquinone glycoside obtained from the Aloe species and exhibits anti-inflammatory and anti-oxidative activities. Here, we aimed to determine the effects of aloin on heme oxygenase-1 (HO-1) induction and on the expressions of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX) 2 in lipopolysaccharide (LPS)-activated human umbilical vein endothelial cells (HUVECs). To the end, aloin was tested whether aloin reduces iNOS protein expression and inflammatory markers (interleukin (IL)-1β and tumor necrosis factor (TNF)-α) in LPS-treated mice lung tissue...
February 12, 2019: Food and Chemical Toxicology
https://read.qxmd.com/read/30768863/human-fibrinogen-for-maintenance-and-differentiation-of-induced-pluripotent-stem-cells-in-two-dimensions-and-three-dimensions
#8
Jarel K Gandhi, Travis Knudsen, Matthew Hill, Bhaskar Roy, Lori Bachman, Cynthia Pfannkoch-Andrews, Karina N Schmidt, Muriel M Metko, Michael J Ackerman, Zachary Resch, Jose S Pulido, Alan D Marmorstein
Human fibrin hydrogels are a popular choice for use as a biomaterial within tissue engineered constructs because they are biocompatible, nonxenogenic, autologous use compatible, and biodegradable. We have recently demonstrated the ability to culture induced pluripotent stem cell (iPSC)-derived retinal pigment epithelium on fibrin hydrogels. However, iPSCs themselves have relatively few substrate options (e.g., laminin) for expansion in adherent cell culture for use in cell therapy. To address this, we investigated the potential of culturing iPSCs on fibrin hydrogels for three-dimensional applications and further examined the use of fibrinogen, the soluble precursor protein, as a coating substrate for traditional adherent cell culture...
February 15, 2019: Stem Cells Translational Medicine
https://read.qxmd.com/read/30768560/regulation-of-endothelial-cell-permeability-by-platelet-derived-extracellular-vesicles
#9
Byron Miyazawa, Alpa Trivedi, Padma Priya Togarrati, Daniel Potter, Gyulnar Baimukanova, Lindsay Vivona, Maximillian Lin, Ernesto Lopez, Rachael Callcut, Amit K Srivastava, Lucy Z Kornblith, Alexander T Fields, Martin A Schreiber, Charles E Wade, John B Holcomb, Shibani Pati
BACKGROUND: Platelet (Plt) derived-extracellular vesicles (Plt-EVs) have hemostatic properties similar to Plts. In addition to hemostasis, Plts also function to stabilize the vasculature and maintain endothelial cell (EC) barrier integrity. We hypothesized that Plt-EVs would inhibit vascular endothelial cell permeability, similar to fresh Plts. To investigate this hypothesis we utilized in vitro and in vivo models of vascular endothelial compromise and bleeding. METHODS: In vitro: Plt-EVs were isolated by ultracentrifugation and characterized for Plt markers and particle size distribution...
February 13, 2019: Journal of Trauma and Acute Care Surgery
https://read.qxmd.com/read/30768435/enhanced-processing-of-von-willebrand-factor-reflects-disease-severity-and-discriminates-severe-portal-hypertension-in-cirrhosis
#10
Lasse L Langholm, Tina Manon-Jensen, Morten A Karsdal, Flemming Bendtsen, Diana J Leeming, Søren Møller
OBJECTIVES: Portal hypertension in cirrhosis is associated with endothelial dysfunction, impaired wound healing, and decreased platelet count. Increased von Willebrand factor (VWF) formation has been suggested as a compensatory mechanism, but the role of VWF processing has not been directly assessed. The aim was to measure the processing of activated VWF (VWF-A) in addition to VWF release (VWF-N) to investigate the association of primary hemostasis with disease activity and portal hypertension in liver cirrhosis...
February 12, 2019: European Journal of Gastroenterology & Hepatology
https://read.qxmd.com/read/30766925/pmab-219-a-monoclonal-antibody-for-the-immunohistochemical-analysis-of-horse-podoplanin
#11
Yoshikazu Furusawa, Shinji Yamada, Shunsuke Itai, Takuro Nakamura, Miyuki Yanaka, Masato Sano, Hiroyuki Harada, Masato Fukui, Mika K Kaneko, Yukinari Kato
Monoclonal antibodies (mAbs) against human, mouse, rat, rabbit, dog, cat, and bovine podoplanin (PDPN), a lymphatic endothelial cell marker, have been established in our previous studies. However, mAbs against horse PDPN (horPDPN), which are useful for immunohistochemical analysis, remain to be developed. In the present study, mice were immunized with horPDPN-overexpressing Chinese hamster ovary (CHO)-K1 cells (CHO/horPDPN), and hybridomas producing mAbs against horPDPN were screened using flow cytometry. One of the mAbs, PMab-219 (IgG2a , kappa), specifically detected CHO/horPDPN cells via flow cytometry and recognized horPDPN protein using Western blotting...
July 2019: Biochemistry and Biophysics Reports
https://read.qxmd.com/read/30766915/evaluation-of-the-topographical-influence-on-the-cellular-behavior-of-human-umbilical-vein-endothelial-cells
#12
Marek Kukumberg, Yuan Yao, Seok Hong Goh, Dawn Jh Neo, Jia Yi Yao, Evelyn Kf Yim
Adhesion and proliferation of vascular endothelial cells are important parameters in the endothelialization of biomedical devices for vascular applications. Endothelialization is a complex process affected by endothelial cells and their interaction with the extracellular microenvironment. Although numerous approaches are taken to study the influence of the external environment, a systematic investigation of the impact of an engineered microenvironment on endothelial cell processes is needed. This study aims to investigate the influence of topography, initial cell seeding density, and collagen coating on human umbilical vein endothelial cells (HUVECs)...
June 2018: Advanced Biosystems
https://read.qxmd.com/read/30765799/using-gjd3-creegfp-mice-to-examine-atrioventricular-node-morphology-and-composition
#13
Samadrita Bhattacharyya, Jialei Duan, Lin Wang, Boxun Li, Minoti Bhakta, Antonio Fernandez-Perez, Gary C Hon, Nikhil V Munshi
The atrioventricular node (AVN) coordinates the timing of atrial and ventricular contraction to optimize cardiac performance. To study this critical function using mouse genetics, however, new reagents are needed that allow AVN-specific manipulation. Here we describe a novel Gjd3-CreEGFP mouse line that successfully recombines floxed alleles within the AVN beginning at E12.5. These mice have been engineered to express CreEGFP under the control of endogenous Gjd3 regulatory elements without perturbing native protein expression...
February 14, 2019: Scientific Reports
https://read.qxmd.com/read/30764688/relationship-between-endothelial-function-and-the-eliciting-shear-stress-stimulus-in-women-changes-across-the-lifespan-differ-to-men
#14
Sophie M Holder, Áine Brislane, Ellen A Dawson, Nicola D Hopkins, Maria T E Hopman, N Timothy Cable, Helen Jones, Tim H A Schreuder, Victoria S Sprung, Louise Naylor, Andrew Maiorana, Andrew Thompson, Dick H J Thijssen, Daniel J Green
Background Premenopausal women have a lower incidence of cardiovascular disease, which may partly be due to a protective effect of estrogen on endothelial function. Animal studies suggest that estrogen may also improve the relationship between shear rate ( SR ) and endothelial function. We aimed to explore the relationship between endothelial function (ie, flow-mediated dilation [ FMD ]) and SR (ie, SR area under the curve [ SRAUC ]) in women versus men, and between pre- versus postmenopausal women. Methods and Results Brachial artery FMD and SRAUC were measured in accordance with expert-consensus guidelines in 932 healthy participants who were stratified into young adults (18-40 years, 389 men, 144 women) and older adults (>40 years, 260 men, 139 women)...
February 19, 2019: Journal of the American Heart Association
https://read.qxmd.com/read/30764675/vegf-a-is-associated-with-early-degenerative-changes-in-cartilage-and-subchondral-bone
#15
Janja Zupan, Peter Vrtačnik, Andrej Cör, Gregor Haring, Georges Weryha, Sophie Visvikis-Siest, Janja Marc
Paired cartilage and subchondral bone of subjects with no clinical history of joint disorders were analyzed to determine whether antioxidant enzymes, inflammatory cytokines and growth factors can be linked to a pre-osteoarthritis. Tissue explants were phenotyped according to Osteoarthritis Research Society International grading and micro-computed tomography, and also screened for the expression of several markers using quantitative polymerase chain reaction. The expression of these same genes was measured in SW1353 cells treated with hydrogen peroxide, to gain insight into the pathways involved with oxidative stress responses...
February 15, 2019: Growth Factors
https://read.qxmd.com/read/30764582/acute-effects-of-hibiscus-sabdariffa-calyces-on-postprandial-blood-pressure-vascular-function-blood-lipids-biomarkers-of-insulin-resistance-and-inflammation-in-humans
#16
Salisu M Abubakar, Moses T Ukeyima, Jeremy P E Spencer, Julie A Lovegrove
BACKGROUND/OBJECTIVES: The acute impact of Hibiscus sabdariffa calyces (HSC) extract on postprandial vascular function and other cardiometabolic risk factors have not been studied previously. This study investigated the acute impact of HSC extract consumption on blood pressure (BP), vascular function and other cardiometabolic risk markers. SUBJECTS/METHODS: Twenty-five men with 1% to 10% cardiovascular disease (CVD) risk (determined by QRISK® 2) were randomised to consume either 250 mL of the aqueous extract of HSC or water with breakfast in a randomised, controlled, single-blinded, 2-meal cross-over study (ClinicalTrials...
February 5, 2019: Nutrients
https://read.qxmd.com/read/30763662/endothelial-cell-derived-extracellular-vesicles-mitigate-radiation-induced-hematopoietic-injury
#17
Sadhna O Piryani, Yiqun Jiao, Angel Y F Kam, Yang Liu, Tuan Vo-Dinh, Benny J Chen, Nelson J Chao, Phuong L Doan
PURPOSE: Extracellular vesicles (EVs) are shed vesicles that bear a combination of nucleic acids and proteins. EVs are becoming recognized as a mode of cell-to-cell communication. Since hematopoietic stem cells (HSCs) reside in proximity to endothelial cells (ECs), we investigated whether EC-derived EVs could regulate HSC regeneration following ionizing radiation. METHODS AND MATERIALS: We generated EVs derived from primary murine marrow ECs. We sought to determine the response of irradiated hematopoietic stem and progenitor cells to syngeneic or allogeneic EVs in culture assays...
February 11, 2019: International Journal of Radiation Oncology, Biology, Physics
https://read.qxmd.com/read/30763573/proteomic-characterization-of-early-lung-response-to-breast-cancer-metastasis-in-mice
#18
Anna Kurpińska, Joanna Suraj, Emilia Bonar, Agnieszka Zakrzewska, Marta Stojak, Magdalena Sternak, Agnieszka Jasztal, Maria Walczak
INTRODUCTION: The tumor-promoting rearrangement of the lungs facilitates the process of cancer cell survival in a foreign microenvironment and enables their protection against immune defense. The study aimed to define the fingerprint of the early rearrangement of the lungs via the proteomic profiling of the lung tissue in the experimental model of tumor metastasis in a murine 4 T1 mammary adenocarcinoma. MATERIALS AND METHODS: The studies were performed on 7-8-week-old BALB/c female mice...
February 11, 2019: Experimental and Molecular Pathology
https://read.qxmd.com/read/30763159/coagulation-changes-induced-by-lower-body-negative-pressure-in-men-and-women
#19
Gerhard Cvirn, James E Waha, Bianca Brix, Andreas Rössler, Andreas Jantscher, Axel Schlagenhauf, Martin Koestenberger, Willibald Wonisch, Thomas Wagner, Nandu Goswami
We investigated whether lower body negative pressure (LBNP) application leads to coagulation activation in whole blood (WB) samples in healthy men and women. Twenty-four women and twenty-one men, all healthy young participants, with no histories of thrombotic disorders and not on medications, were included. LBNP was commenced at -10 mmHg and increased by -10 mmHg every-five minutes until a maximum of -40 mmHg. Recovery up to 10 minutes was also monitored. Blood samples were collected at baseline, at end of LBNP, and end of recovery...
February 14, 2019: Journal of Applied Physiology
https://read.qxmd.com/read/30761739/pecam-1-modulates-liver-damage-induced-by-synergistic-effects-of-tnf-%C3%AE-and-irradiation
#20
Gesa Malik, Jörg Wilting, Clemens Friedrich Hess, Giuliano Ramadori, Ihtzaz Ahmed Malik
The mechanisms of radiation-induced liver damage are poorly understood. We investigated if tumour necrosis factor (TNF)-α acts synergistically with irradiation, and how its activity is influenced by platelet endothelial cell adhesion molecule-1 (PECAM-1). We studied murine models of selective single-dose (25 Gy) liver irradiation with and without TNF-α application (2 μg/mouse; i.p.). In serum of wild-type (wt)-mice, irradiation induced a mild increase in hepatic damage marker aspartate aminotransferase (AST) in comparison to sham-irradiated controls...
February 14, 2019: Journal of Cellular and Molecular Medicine
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