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CSH transplantation

Luanda B F C Souza, Walicyranison P Silva-Rocha, Magda R A Ferreira, Luiz Alberto L Soares, Terezinha I E Svidzinski, Eveline P Milan, Regina H Pires, Ana Marisa Fusco Almeida, Maria José S Mendes-Giannini, Guilherme Maranhão Chaves
This study evaluated the influence of the extract of Eugenia uniflora in adhesion to human buccal epithelial cells (HBEC) biofilm formation and cell surface hydrophobicity (CSH) of Candida spp. isolated from the oral cavity of kidney transplant patients. To evaluate virulence attributes in vitro, nine yeasts were grown in the presence and absence of 1000 μg/mL of the extract. Adhesion was quantified using the number of Candida cells adhered to 150 HBEC determined by optical microscope. Biofilm formation was evaluated using two methodologies: XTT (2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2 H -tetrazolium-5-carboxanilide) and crystal violet assay, and further analyzed by electronic scan microscopy...
September 20, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Masaki Honda, Takayuki Takeichi, Shintaro Hashimoto, Daiki Yoshii, Kaori Isono, Shintaro Hayashida, Yuki Ohya, Hidekazu Yamamoto, Yasuhiko Sugawara, Yukihiro Inomata
Neutrophils are considered responsible for the pathophysiological changes resulting from hepatic ischemia-reperfusion (I/R) injury, which is a complication of trauma, shock, liver resection, and transplantation. Recently, evidence is accumulating that formyl-peptide receptor (FPR) signaling constitutes an important danger signal that guides neutrophils to sites of inflammation. This study aimed to investigate dynamic neutrophil recruitment using two-photon laser-scanning microscopy (TPLSM) in response to FPR1 blockade during hepatic I/R...
February 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
Tzong-fu Kuo, Sheng-Yang Lee, Hong-Da Wu, Malosi Poma, Yu-Wei Wu, Jen-Chang Yang
The purpose of this in vivo study was to evaluate the effect of human dental pulp stem cells (hDPSCs) on various resorbable calcium sulfate/calcium phosphate bone grafts in bone regeneration. Granular particles of calcium sulfate dehydrate (CSD), α-calcium sulfate hemihydrate/amorphous calcium phosphate (α-CSH/ACP), and CSD/β-tricalcium phosphates (β-TCP) were prepared for in vitro dissolution and implantation test. The chemical compositions of specimen residues after dissolution test were characterized by XRD...
May 2015: Materials Science & Engineering. C, Materials for Biological Applications
Toshiyuki Mizota, Shino Matsukawa, Hiroshi Fukagawa, Hiroki Daijo, Tomoharu Tanaka, Fengshi Chen, Hiroshi Date, Kazuhiko Fukuda
OBJECTIVE: To determine the incidence and predisposing factors of hypotension during anesthetic induction in lung transplant recipients. DESIGN: Retrospective study. SETTING: University hospital. PARTICIPANTS: Patients who underwent lung transplantation between 2008 and 2013 (n = 68). INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: The authors analyzed the mean arterial pressure (MAP) from administration of anesthetic drugs to 10 minutes after endotracheal intubation (ie, the anesthetic induction) among participants who underwent lung transplantation...
August 2015: Journal of Cardiothoracic and Vascular Anesthesia
Yu-Yu Tsai, Sea-Fue Wang, Shu-Ting Kuo, Wei-Hsing Tuan
The biodegradation rate of calcium sulfate hemihydrate (CSH) tablets is extremely rapid. This study adopts a water vapor treatment to modify the surface structure of a CSH tablet. After the treatment, a small amount of calcium sulfate dihydrate (CSD) forms on the surface; the porosity near the surface region decreases, consequently enhancing tablet strength and surface hardness. Because of the decrease in porosity at the surface, the time for complete disintegration increases from 1.2 h to 7.2 h; the time for complete dissolution increases from 7 days to 25 days...
January 1, 2013: Materials Science & Engineering. C, Materials for Biological Applications
Xue Han, Hongchen Liu, Dongsheng Wang, Fang Su, Yong Zhang, Wei Zhou, Shujun Li, Rui Yang
BACKGROUND: Lack of osseointegration between a dental implant and the walls of the alveolar bone is a common problem in immediate implantation. Injectable tissue-engineered bone (ITB) may be an effective and minimally invasive solution to the problem. In this study, an injectable bone cement, nHAC/CSH, which consists of nano-hydroxyapatite/collagen (nHAC) and calcium sulfate hemihydrate (CaSO4 .½H2 O; CSH) was investigated as a tissue-engineered scaffold material with blood-acquired mesenchymal progenitor cells (BMPC) as seeding cells...
June 2013: Clinical Implant Dentistry and related Research
Shah H A Mahdi, Shinichi Gima, Yuya Tomita, Hideo Yamasaki, Joji M Otaki
Butterfly wing color patterns can be modified by the application of temperature shock to pupae immediately after pupation, which has been attributed to a cold-shock-induced humoral factor called cold-shock hormone (CSH). Here, we physiologically characterized CSH and pharmacological action of tungstate, using a nymphalid butterfly Junonia orithya. We first showed that the precise patterns of modification were dependent on the time-point of the cold-shock treatment after pupation, and confirmed that the modification properties induced in a cold-shocked pupa were able to be transferred to another pupa in a parabiosis experiment...
September 2010: Journal of Insect Physiology
Anna Michalska, Eugenia Gospodarek
Enterobacter sp. is one of the important factors of hospital infections, especially at patients in intensive care, surgical and transplant units and cell surface hydrophobicity (CSH) plays an important role in the pathogenicity of many bacterial. Because of the increase of these bacteria in the infections, the aims of this study were the assessment of CSH of Enterobacter sp. strains on the basis of two methods: salt aggregation test (SAT) and bacterial adhesion to hydrocarbons (BATH). 175 strains of Enterobacter sp...
2009: Medycyna Doświadczalna i Mikrobiologia
Min-min Wang, Shi-jun Chen, Qi-fa Ye, Yi-jun Yang, Shi-bin Chen, Xin-min Zhou, Li-min Guo, Yue-xin Zhang, Xiao-qiang Ding, Xiao-bin Hu, Hong-tao Luo, Yi-he Liu, Wen-ya Wang
BACKGROUND: A liver support therapy, named molecular adsorbents recirculating system (MARS), has been used for more than 700 liver failure patients in China. We made here a summary to evaluate the effects of MARS treatment in different applications with emphasis on hepatitis B virus (HBV) based liver failure. METHODS: This report analyzed data of 252 patients (mean age (44.9+/- 12.7) years) in three groups: acute severe hepatitis (ASH), subacute severe hepatitis (SSH) and chronic severe hepatitis (CSH)...
November 5, 2008: Chinese Medical Journal
Jeffrey J Gaynor, Jang I Moon, Tomoaki Kato, Seigo Nishida, Gennaro Selvaggi, David M Levi, Eddie R Island, Nikolaos Pyrsopoulos, Debbie Weppler, Susan Ganz, Phillip Ruiz, Andreas G Tzakis
BACKGROUND: In orthotopic liver transplantation (OLT) distinct causes of graft failure (GF) and death with a functioning graft (DFG) exist. Prognostic factors for one failure type may be distinctly different from those predictive of other types, and an accurate portrayal of these relationships may more clearly explain each factor's importance. METHODS: A multivariable cause-specific hazard (CSH) rate analysis using Cox stepwise regression was performed among 877 adults who received primary OLT during 1996-2004 with tacrolimus+steroids as immunosuppression...
July 27, 2007: Transplantation
K Weichman, L M Dember, T Prokaeva, D G Wright, K Quillen, M Rosenzweig, M Skinner, D C Seldin, V Sanchorawala
Light chain deposition disease (LCDD) is caused by a clonal plasma cell disorder in which fragments of monoclonal immunoglobulin light chains form non-fibrillary deposits in various tissues resulting in organ dysfunction. Crystal storing histiocytosis (CSH) is another light chain deposition disorder in which monoclonal light chains form intracytoplasmic crystals. Both are uncommon diseases for which there is limited treatment experience. Between 2003 and 2005, five patients with LCDD and one with CSH were treated at Boston University Medical Center with high-dose melphalan and autologous peripheral blood stem cell transplantation (HDM/SCT)...
September 2006: Bone Marrow Transplantation
Jeffrey J Gaynor, Tomoaki Kato, Gennaro Selvaggi, Jang I Moon, David M Levi, Seigo Nishida, Juan R Madariaga, Debbie Weppler, Phillip Ruiz, Andreas G Tzakis
BACKGROUND: Although graft and patient survival are vital in reporting overall results of clinical transplant studies, these outcomes do not account for distinct types of graft failure and death, which clearly exist in pediatric small intestine transplantation (Itx). The use of a cause-specific hazard (CSH) approach may provide more precise identification and thus greater insight as to why certain factors are prognostically important. METHODS: Among 119 pediatric patients who received primary Itx at our center since 1994, Cox model stepwise regression analyses were performed to identify prognostic factors for the following CSH rates: intestinal graft failure (IGF)/death due to rejection, death due to infection not triggered by IGF, and intestinal graft loss/death due to other causes...
April 27, 2006: Transplantation
Danila Di Giuseppe, Simona Frosali, Raffaella Priora, Francesca Cherubini Di Simplicio, Giuseppe Buonocore, Carla Cellesi, Pier Leopoldo Capecchi, Franco Laghi Pasini, Pietro Enea Lazzerini, Hieronim Jakubowski, Paolo Di Simplicio
We assayed the redox forms of cysteine (reduced [CSH], oxidized [CSSC], and bound to protein [CS-SP]), cysteinylglycine (CGSH; cysteinylgycine disulfide [CGSSGC] and cysteinylglycine-protein mixed disulfide [CGS-SP]), glutathione (GSH; glutathione disulfide [GSSG] and glutathione-protein mixed disulfide [GS-SP]), homocysteine (Hcy; homocystine [HcyS] and homocystine-protein mixed disulfides [bHcy]), and protein sulfhydryls in the plasma of healthy subjects (divided into 8 groups ranging in age from birth to 70 years) and patients with mild hyperhomocysteinemia associated with cardiovascular disease (heart-transplant patients) or vascular atherosclerosis, with or without renal failure...
November 2004: Journal of Laboratory and Clinical Medicine
N S Nguyen, S M Pulido, U T Rüegg
The immunosuppressive drug cyclosporin A (CsA) depresses neutrophil oxidative burst which may lead to an increased susceptibility to infection in transplant patients. Using specific CsA analogues we investigated the mechanism of inhibition of the oxidative burst and evaluated short and long-term effects of CsA on dimethylsulphoxide-differentiated HL-60 neutrophils. A biphasic pattern was observed: a 4 h pre-treatment with CsA (1 microM) diminished the fMLP induced [Ca2+]c rise, reactive oxygen species (ROS) production, and beta-glucuronidase release by about 40%, whereas a 20 h pre-treatment increased these responses by about 1...
August 1998: British Journal of Pharmacology
D Chabannes, J F Moreau, J P Soulillou
The effect of cyclosporine and metabolite 17 (M17) as well as other CsA-related compounds (CsG, dihydro-CsC, dihydro-CsD, CsH, B5.49, and H7.94) was tested on T lymphocyte clone proliferation. In these experiments, antigen and interleukin 2 (IL-2) dependent long-term T lymphocyte clones derived from a rejected human kidney graft infiltrate were used. They were specifically committed (proliferation and cytotoxicity) for the donor Epstein-Barr virus (EBV)-transformed cells. CsA strongly inhibited clone T cell proliferation induced by the antigen...
December 1987: Transplantation
Y J Ganem, J M Launay, M C Debons-Guillemin, J Lasneret, A M Roucayrol, J Lesser, G Peries, C Dreux
The first successful heterotransplantation of a human carcinoid tumor into nude mice is reported. CSH, a voluminous hepatic metastasis of a primary bronchial carcinoid tumor (CSB) was resected and transplanted into three irradiated nude (Swiss-nu/nu) mice both by subcutaneous (SC) and intramuscular (IM) routes; the success rate was five of six. Heterotransplanted tumors took 4 to 5 months to appear in the mice and 1 month to attain a width of 0.5 cm. Both human and mouse tumors (named CSH-SC and CSH-IM) were studied by light and electron microscopy...
August 15, 1991: Cancer
C Amsellem, M Haftek, J Kanitakis, J Thivolet
Cyclosporin A (CsA) was first used in organ transplantation and for the treatment of autoimmune disorders because of its strong immunosuppressant properties. Several laboratory studies have demonstrated that CsA exerts an inhibitory action on the growth of various cell types in culture, including human skin cells. Such an influence on epidermal keratinocytes, if not associated with the serious adverse effects of CsA medication, would be of interest for the treatment of hyperproliferative genodermatoses such as non-bullous congenital ichthyotic erythroderma (NBCIE)...
1992: Archives of Dermatological Research
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