Megi Rexhepaj, Young-Jun Park, Lisa Perruzza, Daniel Asarnow, Matthew McCallum, Katja Culap, Christian Saliba, Giada Leoni, Alessio Balmeli, Courtney Yoshiyama, Miles S Dickinson, Joel Quispe, Jack T Brown, M Alejandra Tortorici, Kaitlin R Sprouse, Ashley L Taylor, Tyler N Starr, Davide Corti, Fabio Begnini, David Veesler
Porcine deltacoronavirus (PDCoV) spillovers were recently detected in children with acute undifferentiated febrile illness, underscoring recurrent zoonoses of divergent coronaviruses. To date, no vaccines or specific therapeutics are approved for use in humans against PDCoV. To prepare for possible future PDCoV epidemics, we isolated human spike (S)-directed monoclonal antibodies from transgenic mice and found that two of them, designated PD33 and PD41, broadly neutralized a panel of PDCoV variants. Cryo-electron microscopy structures of PD33 and PD41 in complex with the PDCoV receptor-binding domain and S ectodomain trimer provide a blueprint of the epitopes recognized by these mAbs, rationalizing their broad inhibitory activity...
April 1, 2024: bioRxiv