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Jiantai Timothy Qiu, Donia Alson, Ta-Hsien Lee, Ching-Chou Tsai, Ting-We Yu, Yu-Sing Chen, Ya-Fang Cheng, Chu-Chi Lin, Scott C Schuyler
Ectopic expression of codon-modified granulocyte-macrophage colony-stimulating factor (cGM-CSF) in TC-1 cells (TC-1/cGM-CSF), a model cell line for human papillomavirus (HPV)-infected cervical cancer cells, increased the expression level of GM-CSF and improved the efficacy of tumor cell-based vaccines in a cervical cancer mouse model. The number of vaccine doses required to induce a long-term immune response in a cervical cancer mouse model is poorly understood. Here, we investigated one, three, and five doses of the irradiated TC-1/cGM-CSF vaccine to determine which dose was effective in inducing a greater immune response and the suppression of tumors...
March 15, 2019: Cancers
Jamile R Silva, Natiely S Sales, Mariângela O Silva, Luana R M M Aps, Ana C R Moreno, Elaine G Rodrigues, Luís C S Ferreira, Mariana O Diniz
The presence of IL-10, produced either by tumor cells or immunosuppressive cells, is frequently associated with a poor prognosis for cancer progression. It may also negatively impact anticancer treatments, such as immunotherapies, that otherwise would promote the activation of cytotoxic T cells capable of detecting and destroying malignant cells. In the present study, we evaluated a new adjuvant approach for anticancer immunotherapy using a plasmid vector encoding a soluble form of the IL-10 receptor (pIL-10R)...
February 26, 2019: Cancer Immunology, Immunotherapy: CII
Malgorzata Lipowska, Jeffrey Klenc, Andrew T Taylor, Luigi G Marzilli
Ligands that coordinate via dianionic phosphonate groups have not been widely utilized in radiopharmaceuticals. N -(phosphonomethyl)iminodiacetic acid ( 1 , PMIDA) and N- (phosphonomethyl)glycine ( 2 , PMG) were investigated as new chelators for the 99m Tc/Re-tricarbonyl "core" ( fac -M(CO)3 , M = 99m Tc, Re) present in a major class of radiopharmaceuticals. fac-M(CO)3 (PMIDA) and fac-M(CO)3 (PMG) complexes were studied by HPLC and 1 H/13 C/31 P NMR methods for M = Re ( Re-1 and Re-2 ) and by HPLC for M = 99m Tc ( 99m Tc-1 and 99m Tc-2 )...
February 24, 2019: Inorganica Chimica Acta
Azam Bolhassani, Sepideh Shahbazi, Elnaz Agi, Nooshin Haghighipour, Amin Hadi, Fatemeh Asgari
Immunotherapy with DCs as antigen-presenting vehicles have already improved patients' outcome against a variety of tumors. Moreover, MSCs were recently used to develop anti-cancer therapeutic or anti-microbial prophylactic vaccines. The current study evaluated immune responses and anti-tumor effects generated by DCs and MSCs derived from mouse bone marrow which were modified with small heat shock proteins 27 and 20 (sHsp27 and sHsp20) and also E7 oncoprotein in tumor mouse model. Two vaccination strategies were utilized including homologous DC or MSC prime/ DC or MSC boost, and heterologous MSC or DC prime/ protein boost vaccinations...
February 22, 2019: Molecular Immunology
Pradnya Parab, Deepak Singh, Santosh Haram, R P Singh, Sangita Bose
Re6 Zr, a non-centrosymmetric superconductor is an interesting system as recent experimental evidence suggests that the superconducting state breaks time reversal symmetry. This implies a mixing of spin singlet-triplet states leading to a complex order parameter in this system. Here, we report point contact Andreev Reflection (PCAR) measurements on a single crystal of Re6 Zr (superconducting transition temperature (Tc ) = 6.78 K). We observe multiple gap features in the PCAR spectra which depends on the type of tip and contact...
February 21, 2019: Scientific Reports
Marina Sagnou, Barbara Mavroidi, Antonio Shegani, Maria Paravatou-Petsotas, Catherine P Raptopoulou, Vassilis Psycharis, Ioannis Pirmettis, Minas S Papadopoulos, Maria Pelecanou
The synthesis and evaluation of three novel 99m Tc complexes (99m Tc-1 - 99m Tc-3), and their corresponding Re complexes (Re-1 - Re-3) in which the phenyl ring of 2-phenylbenzothiazole or 2-phenylbenzimidazole is replaced by the cyclopentadienyl tricarbonyl [Cp 99m Tc(CO)3 ] core, is reported. Both 99m Tc and Re complexes were prepared from the corresponding ferrocenyl derivatives and the Re complexes were fully characterized by elemental analysis, spectroscopic methods and X-ray crystallography. The complexes exhibit effective in vitro binding to β-amyloid (Aβ) plaques and fibrils, inhibit Aβ fibril formation, significantly reduce Aβ-induced cytotoxicity and ROS production in neuronal cell cultures...
February 15, 2019: Journal of Medicinal Chemistry
Li Cai, Bingjie Ma, Lizi Lin, Yajun Chen, Wenhan Yang, Jun Ma, Jin Jing
With the increasing number of the one-child family, it is important to investigate whether the only-child status is associated with dyslipidemia. Among a national sample of 65,347 Chinese children aged 6-17 years, 16,100 lipid profiles were available. Children's height, weight, total cholesterol (TC), triglycerides (TG), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) were measured. In comparison to children with siblings, only children (OC) were more likely to be boys and live in urban areas...
February 5, 2019: Scientific Reports
Charlotte M Mousset, Willemijn Hobo, Rob Woestenenk, Frank Preijers, Harry Dolstra, Anniek B van der Waart
The T cell compartment can form a powerful defense against extrinsic (e.g., pathogens) and intrinsic danger (e.g., malignant cells). At the same time, specific subsets of T cells control this process to keep the immune system in check and prevent autoimmunity. A wide variety in T cell functionalities exists, which is dependent on the differentiation and maturation state of the T cells. In this review, we report an overview for the identification of CD4+ T-αβ cells (T-helper (Th)1, Th2, Th9, Th17, Th22, and CD4+ regulatory T cells), CD8+ T-αβ cells (cytotoxic T lymphocyte (Tc)1, Tc2, Tc9, Tc17, and CD8+ regulatory T cells), and their additional effector memory status (naïve, stem cell memory, central memory, effector memory, and effector) using flow cytometry...
February 4, 2019: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Chung-Man Moon, Sang-Soo Shin, Suk-Hee Heo, Hyo-Soon Lim, Myeong-Ju Moon, Suchithra Poilil Surendran, Ga-Eon Kim, Il-Woo Park, Yong-Yeon Jeong
PURPOSE: The objective was to assess metabolic changes in different stages of liver fibrosis using hyperpolarized C-13 magnetic resonance spectroscopy (MRS) and metabolic imaging. PROCEDURES: Mild and severe liver fibrosis were induced in C3H/HeN mice (n = 14) by injecting thioacetamide (TAA). Other C3H/HeN mice (n = 7) were injected with phosphate buffer saline (PBS) (7.4 pH) as normal controls. Hyperpolarized C-13 MRS was performed on the livers of the mice, which was accompanied by intravoxel incoherent motion (IVIM) diffusion-weighted imaging with 12 b values...
January 28, 2019: Molecular Imaging and Biology: MIB: the Official Publication of the Academy of Molecular Imaging
Rodolfo Garza-Morales, Jose J Perez-Trujillo, Elvis Martinez-Jaramillo, Odila Saucedo-Cardenas, Maria J Loera-Arias, Aracely Garcia-Garcia, Humberto Rodriguez-Rocha, Esma Yolcu, Haval Shirwan, Jorge G Gomez-Gutierrez, Roberto Montes-de-Oca-Luna
The SA-4-1BBL, an oligomeric novel form of the natural ligand for the 4-1BB co-stimulatory receptor of the tumor necrosis factor (TNF) superfamily, as a recombinant protein has potent pleiotropic effects on cells of innate, adaptive, and regulatory immunity with demonstrated therapeutic efficacy in several tumor models. However, the production of soluble form of SA-4-1BBL protein and quality control is time and resource intensive and face various issues pertinent to clinical development of biologics. The present study sought to take advantage of the simplicity and translatability of DNA-based vaccines for the production and delivery of SA-4-1BBL for cancer immune prevention and therapy...
January 15, 2019: Cancers
Jia Zheng, Zhaoqing Sun, Xingang Zhang, Zhao Li, Xiaofan Guo, Yanxia Xie, Yingxian Sun, Liqiang Zheng
BACKGROUND: Studies have shown that non-traditional lipid profiles have a better association with stroke than traditional blood lipids in clinical applications, other studies have drawn different conclusions. METHODS: This study was a large-scale study with a median follow-up of 8.4 years. The hazard ratio (HR) and 95% Confidence interval (CI) of lipid variables for risk of incident stroke were analyzed by multivariable Cox proportional hazard models. RESULTS: During the follow-up, 502 new strokes (310 ischemic, 187 hemorrhagic, and 5 unclassified strokes) occurred among the 5099 hypertensive patients...
January 8, 2019: Lipids in Health and Disease
Yi-Shu Tang, Dongmei Wang, Chunxia Zhou, Shuren Zhang
Bacterial magnetosomes (BMs) are used as carriers for antibodies, enzymes, and nucleic acids. This study aimed to demonstrate the clinical utility of BMs derived from Magnetospirillum gryphiswaldense for use in anti-tumor immunotherapy. Bis(sulfosuccinimidyl) suberate (BS3) was used to prepare BM-anti-4-1BB antibody complexes. We used syngeneic TC-1 mouse models of cancer to investigate whether BMs combined with an anti-4-1BB agonistic antibodies could enhance the therapeutic effects of anti-4-1BB antibodies in localized disease settings...
January 1, 2019: Biotechnology and Applied Biochemistry
Alireza Mohebbi, Mir Saeed Ebrahimzadeh, Sanaz Baghban Rahimi, Mohsen Saeidi, Alijan Tabarraei, Seyed Reza Mohebbi, Sadegh Shirian, Ali Gorji, Amir Ghaemi
The potential of non-replicating Newcastle Disease Virus (NDV) as an adjuvant for DNA vaccination remains to be elucidated. To assess the therapeutic effects of DNA vaccine (HPV-16 E7 gene) adjuvanted with NDV, female C57/BL6 mice were inoculated with murine TC-1 cells of human papillomavirus (HPV)-related carcinoma, expressing human papillomavirus 16 (HPV-16) E6/E7 antigens, and immunized with DNA vaccine alone or pretreated with NDV. One week after third immunization, Cytotoxic T lymphocytes (CTLs), splenocyte proliferation, cytokine balance (IFN-γ, IL-4 and IL-12 secretions) and intratumoral expression of cytotoxicity related proteins in tumor lysates were investigated...
February 2019: Virus Research
Alireza Tahamtan, Mohammad Barati, Alijan Tabarraei, Seyed Reza Mohebbi, Sadegh Shirian, Ali Gorji, Amir Ghaemi
BACKGROUND: In recent years attention has been paid to develop effective adjuvant systems for DNA vaccines. Co-formulation of a gene delivery vector with an immunostimulator can enhance therapeutic efficiency of DNA vaccine. OBJECTIVE: To investigate the efficacy of chitosan as a nanodelivery system to enhance antitumor effects of human papilloma virus (HPV)-16 DNA vaccine with IL-12 gene for protection against TC-1 tumor using an animal model. METHODS: The mice were challenged by subcutaneous injection of TC-1 cells and immunized intramuscularly with DNA vaccine thrice at seven-day intervals...
December 2018: Iranian Journal of Immunology: IJI
Amin Hadi, Abbas Rastgoo, Nooshin Haghighipour, Azam Bolhassani, Fatemeh Asgari, Sepehr Soleymani
Intracellular delivery of DNA is considered a challenge in biological research and treatment of diseases. The previously reported transfection rate by commercially available transfection reagents in cancer cell lines, such as the mouse lung tumor cell line (TC-1), is very low. The purpose of this study is to introduce and optimize an efficient gene transfection method by mechanical approaches. The combinatory transfection effect of mechanical treatments and conventional chemical carriers is also investigated on a formerly reported hard-to-transfect cell line (TC-1)...
2018: PloS One
Qing Ban, Wuya Chen, Shiwei Du, Huimin Wang, Jian Li, Rong You, Ying Jin, Yongyong Xue, Yan-Qing Guan
Lung is one of important organs and lung diseases seriously affect the health of human beings. In this study, chitosan and gelatin as natural biological macromolecules raw material for the synthesis of ordered colloidal crystal scaffolds (CCS), Fe3 O4 magnetic nanoparticles (MNPs) were used as pore-making for the first time. The pore-making agent were added into the hydrogels to synthesis the ordered (magnetic field) and disordered (no magnetic field) CCS. Collagen and basic fibroblast growth factor (bFGF) modified on the surface of CCS...
January 1, 2019: Colloids and Surfaces. B, Biointerfaces
Farideh Tabatabaei Yazdi, Abbas Tanhaeian, Marjan Azghandi, Alireza Vasiee, Behrooz Alizadeh Behbahani, Seyed Ali Mortazavi, Sahar Roshanak
The antimicrobial peptide Thrombocidin-1 (TC-1) isolated from human blood that derived from NAP-2 by deleting of two amino acids from C-terminal region. In this study, a C-terminal 6 _ His tagged recombinant TC-1 was expressed as a secreted peptide in Pichia pastoris, for the first time. The recombinant P. pastoris was inoculated in to BMMY culture medium, incubation with 5 μl/ml absolute methanol for 72 h at 30 °C. The TC-1 peptide was concentrated with nickel affinity chromatography and electrophoresis on 16% acrylamide gels...
December 1, 2018: Microbial Pathogenesis
Adrianna Grzelak, Ingrid Polakova, Jana Smahelova, Julie Vackova, Lucie Pekarcikova, Ruth Tachezy, Michal Smahel
Combined immunotherapy constitutes a novel, advanced strategy in cancer treatment. In this study, we investigated immunotherapy in the mouse TC-1/A9 model of human papillomavirus type 16 (HPV16)-associated tumors characterized by major histocompatibility complex class I (MHC-I) downregulation. We found that the induction of a significant anti-tumor response required a combination of DNA vaccination with the administration of an adjuvant, either the synthetic oligodeoxynucleotide ODN1826, carrying immunostimulatory CpG motifs, or α-galactosylceramide (α-GalCer)...
November 21, 2018: International Journal of Molecular Sciences
Jinyu Li, Adila Aipire, Huixin Zhao, Pengfei Yuan, Jinyao Li
We previously found that Pleurotus ferulae polysaccharides (PFPS) improved the maturation and function of dendritic cells (DCs). In this study, we investigated the effects of PFPS on the antitumor efficacy of therapeutic human papillomavirus (HPV) DC-based vaccine. PFPS stimulated DCs pulsed with HPV E6/E7 peptides were used to treat tumor mice on day 5 & 12 (HPV + PFPS-DCs early) and day 12 & 19 (HPV + PFPS-DCs late) after TC-1 cell injection. Compared to control group, both HPV + PFPS-DCs early and HPV + PFPS-DCs late strategies significantly inhibited tumor growth, which was significantly correlated with the increased activation status of both CD4+ and CD8+ T cells, the decreased frequencies of myeloid-derived suppressor cells, and the induction of HPV-specific CD8+ T cell responses...
November 14, 2018: Human Vaccines & Immunotherapeutics
A R Khosravi, S Alheidary, D Nikaein, N Asghari
Aspergillus fumigatus conidia are the most prevalent indoors fungal allergens. The interaction between Aspergillus antigens and lung epithelial cells (LECs) result in innate immune functions. The association between Aspergillus conidia and allergic reactions, like allergic bronchopulmonary aspergillosis (ABPA) and asthma have been repeatedly reported. Since conventional therapies for allergy and asthma are limited, finding new promising treatments are inevitable. This study was designed to evaluate the effect of A...
October 22, 2018: Journal de Mycologie Médicale
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