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Prevention of adhesion formation

Anne E C Nichols, Katherine T Best, Alayna E Loiselle
Tendon injuries are common and can dramatically impair patient mobility and productivity, resulting in a significant socioeconomic burden and reduced quality of life. Because the tendon healing process results in the formation of a fibrotic scar, injured tendons never regain the mechanical strength of the uninjured tendon, leading to frequent reinjury. Many tendons are also prone to the development of peritendinous adhesions and excess scar formation, which further reduce tendon function and lead to chronic complications...
February 8, 2019: Translational Research: the Journal of Laboratory and Clinical Medicine
Amir Horiquini Barbosa, Jaqueline Lopes Damasceno, Luciana Assirati Casemiro, Carlos Henrique Gomes Martins, Regina Helena Pires, Regina Célia Candido
PURPOSE: To isolate Candida spp. from dental prosthesis users' saliva and to evaluate the isolates for the presence of several virulence factors. This research also aimed to investigate the antifungal activity of 3 commercial mouthwashes/oral antiseptic formulations containing 0.12% chlorhexidine, 0.07% cetylpyridinium, or 0.075% cetylpyridinium against planktonic and sessile (biofilm mode) yeast cells. MATERIALS AND METHODS: Forty-three Candida yeasts were isolated from 32 of 70 selected patients, and the virulence factors of C...
February 15, 2019: Journal of Prosthodontics: Official Journal of the American College of Prosthodontists
Minji Kang, Sungwhan Kim, Heejin Kim, Youngjun Song, Dongwook Jung, Sunah Kang, Ji-Hun Seo, Sohee Nam, Yan Lee
Quorum sensing (QS) inhibitor-based therapy is an attractive strategy to inhibit bacterial biofilm formation without excessive induction of antibiotic resistance. Thus, we designed Ca2+ -binding poly(lactide- co-glycolide) (PLGA) microparticles that can maintain a sufficient concentration of QS inhibitors around hydroxyapatite (HA) surfaces in order to prevent biofilm formation on HA-based dental or bone tissues or implants and, therefore, subsequent pathogenesis. Poly(butyl methacrylate- co-methacryloyloxyethyl phosphate) (PBMP) contains both Ca2+ -binding phosphomonoester groups and PLGA-interacting butyl groups...
February 15, 2019: ACS Applied Materials & Interfaces
Myung-Jin Lee, Jae-Sung Kwon, Heng Bo Jiang, Eun Ha Choi, Gyungsoon Park, Kwang-Mahn Kim
Titanium is commonly used as a biomaterial for dental implants. In this study, we investigated the antibacterial properties of titanium samples following treatment with a non-thermal atmospheric pressure plasma jet (NTAPPJ) on bacteria with two different cell wall structures, including gram-positive and gram-negative bacteria. The hydrophilicity and surface energy of titanium surfaces were significantly increased after NTAPPJ treatment without altering topographical features. Changes in the chemical composition and reductive potential were observed on the NTAPPJ-treated titanium surfaces...
February 13, 2019: Scientific Reports
Anne-Laure Papa, Amanda Jiang, Netanel Korin, Michelle B Chen, Erin T Langan, Anna Waterhouse, Emma Nash, Jildaz Caroff, Amanda Graveline, Andyna Vernet, Akiko Mammoto, Tadanori Mammoto, Abhishek Jain, Roger D Kamm, Matthew J Gounis, Donald E Ingber
Platelets are crucial for normal hemostasis; however, their hyperactivation also contributes to many potentially lethal pathologies including myocardial infarction, stroke, and cancer. We hypothesized that modified platelets lacking their aggregation and activation capacity could act as reversible inhibitors of platelet activation cascades. Here, we describe the development of detergent-extracted human modified platelets (platelet decoys) that retained platelet binding functions but were incapable of functional activation and aggregation...
February 13, 2019: Science Translational Medicine
Kurt Reynolds, Priyanka Kumari, Lessly Sepulveda Rincon, Ran Gu, Yu Ji, Santosh Kumar, Chengji J Zhou
Diverse signaling cues and attendant proteins work together during organogenesis, including craniofacial development. Lip and palate formation starts as early as the fourth week of gestation in humans or embryonic day 9.5 in mice. Disruptions in these early events may cause serious consequences, such as orofacial clefts, mainly cleft lip and/or cleft palate. Morphogenetic Wnt signaling, along with other signaling pathways and transcription regulation mechanisms, plays crucial roles during embryonic development, yet the signaling mechanisms and interactions in lip and palate formation and fusion remain poorly understood...
February 4, 2019: Disease Models & Mechanisms
Matej Vicen, Barbora Vitverova, Radim Havelek, Katerina Blazickova, Miloslav Machacek, Jana Rathouska, Iveta Najmanová, Eva Dolezelova, Alena Prasnicka, Magdalena Sternak, Carmelo Bernabeu, Petr Nachtigal
Our objective was to investigate the effect of cholesterol [hypercholesterolemia and 7-ketocholesterol (7K)] on endoglin (Eng) expression and regulation with respect to endothelial or vascular dysfunction in vivo and in vitro. In vivo experiments were performed in 2-mo-old atherosclerosis-prone apolipoprotein E-deficient/LDL receptor-deficient (ApoE-/- /LDLR-/- ) female mice and their wild-type C57BL/6J littermates. In in vitro experiments, human aortic endothelial cells (HAECs) were treated with 7K. ApoE-/- /LDLR-/- mice developed hypercholesterolemia accompanied by increased circulating levels of P-selectin and Eng and a disruption of NO metabolism...
February 12, 2019: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Chen Sai, Jiang Yunhan, Jian Zhao, Zhu Yu, Zhu Yun, Cao Zhezhe, Tang Fuqin, Ma Ruiyan, Xiao Yingbin
Atrial fibrillation has caused severe burden for people worldwide. Differentiation of fibroblasts into myofibroblasts, and consequent progress in atrial structural remodeling have been considered the basis for persistent atrial fibrillation, yet little is known about the molecular mechanisms underlying the process. Here, we show that cyclin-dependent kinase 1 (CDK1) is activated in atrial fibroblasts from patients with atrial fibrillation (AFPAF) and in platelet derived growth factor BB (PDGF-BB)-treated atrial fibroblasts from patients with sinus rhythm (AFPSR)...
February 8, 2019: International Heart Journal
Andrea S Rothmeier, Henri H Versteeg, Wolfram Ruf
BACKGROUND: Cell injury signal-induced activation and release of tissue factor (TF) on extracellular vesicles (EV) from immune and vessel wall cells propagate local and systemic coagulation initiation. TF trafficking and release on EV occurs in concert with the release of cell adhesion receptors, including integrin β1 heterodimers which control trafficking of the TF-FVIIa complex. Activation of the TF signaling partner, protease activated receptor (PAR) 2, also triggers TF release on integrin β1+ EV from endothelial cells, but the physiological signals for PAR2-dependent EV generation at the vascular interface remain unknown...
February 11, 2019: Journal of Thrombosis and Haemostasis: JTH
Yufeng Zhou, Kai Yang
INTRODUCTION: Surgery or trauma may induce extensive arthrofibrosis around joints and tendon for the restrictive range of motion. Although some approaches were proposed, this problem is not solved satisfactorily. Extracorporeal shock wave therapy (ESWT) has been used for orthopedic, musculoskeletal, and fibrotic disorders. Whether it could prevent the formation of arthrofibrosis during the joint repair is unknown. METHODS: Intra-articular adhesions were created in the right knee of the rabbit by cortical bone shaving and subsequent cast immobilization...
February 2, 2019: Injury
Shoji Takenaka, Tatsuya Ohsumi, Yuichiro Noiri
Therapeutic mouthwash (MW) is an adjunctive tool along with a regular oral hygiene routine of daily tooth brushing and daily flossing. Previous systematic reviews have demonstrated that it is effective against dental biofilm and gingival inflammation, for prevention of dental caries, and for managing one's bad breath condition according to the active ingredients. MWs prevent the microorganisms from bacterial adhesion that corresponds to the initial step in biofilm formation. This review summarized the current state of evidence such as anti-biofilm, anti-gingivitis and cariostatic properties of MWs by evaluating systematic reviews from the past six years...
November 2019: Japanese Dental Science Review
Kymora B Scotland, Joey Lo, Thomas Grgic, Dirk Lange
Ureteral stents are commonly used devices in hospital settings. However, their usage is often complicated by associated urinary tract infections as a result of bacterial adhesion onto the indwelling implant surfaces, followed by the formation of layers of biofilm. Once formed, the biofilm is exceedingly difficult to remove, potentially leading to further morbidity and even urosepsis. Urosepsis, where pathogens from the urinary tract enter the bloodstream, has a mortality rate of up to 50% of severely infected patients...
February 8, 2019: Biofouling
Chuan Wan, Le-Tian Wang, Jun-Yi Sha, Hong-Hua Ge
Nanofluids have great application prospects in industrial heat exchange systems because they can significantly improve the heat and mass transfer efficiency. However, the presence of nanoparticles in the fluid might also affect the formation and attachment of inorganic scales, such as calcium carbonate, on the heat exchange surface. The effects of carbon nanoparticles on the crystallization of calcium carbonate in aqueous solution were studied by the scale inhibition test, solution analysis, scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Fourier transform infrared spectroscopy (FTIR)...
February 1, 2019: Nanomaterials
Songtao Wu, Naoki Uyama, Rei Atono Itou, Etsuro Hatano, Hiroko Tsutsui, Jiro Fujimoto
Daikenchuto (DKT) has been widely used for the treatment of postsurgical ileus in Japan. However, its effect on postsurgical adhesion formation has been obscure. In this study, the effect of DKT on postsurgical adhesion formation induced by cecum cauterization or cecum abrasion in mice was investigated. First, the expression of adhesion-related molecules in damaged ceca was investigated by quantitative (q)RT-PCR. During 24 h after surgery, mRNA expressions of interferon-γ (IFN-γ), plasminogen activator inhibitor-1 (PAI-1), interleukin-17 (IL-17), and Substance P (SP) in cauterized ceca and those of PAI-1 and IL-17 in abraded ceca were significantly up-regulated...
2019: Biological & Pharmaceutical Bulletin
Marc Miserez, An P Jairam, Geesien S A Boersema, Yves Bayon, Johannes Jeekel, Johan F Lange
BACKGROUND: Prosthetic materials for the repair of abdominal wall defects have been studied extensively to improve outcome. A new approach can be the use of a slowly resorbable synthetic mesh, which aims to combine advantages of both synthetic and biological meshes. The objective of this review is to give an overview of the physicochemical characteristics and biomechanical, histological, and macroscopic outcome (recurrence, adhesion formation) of the use of resorbable synthetic meshes, for treatment and prevention of abdominal wall hernias, based on experimental studies...
January 30, 2019: Journal of Surgical Research
S Gordts, R Campo
Formation of the ovarian endometrioma consists of implantation, invagination of the ovarian cortex, and adhesion formation. Progression is characterized by repeated injury and repair with degenerative changes. Already with a partially deprived ovarian reserve, resulting from the disease, surgical treatment carries a potential risk of further follicular deprivation. Surgery should therefore be performed with microsurgical precision by experienced hands. Early treatment can possibly prevent further progression...
January 5, 2019: Best Practice & Research. Clinical Obstetrics & Gynaecology
Joseph S Fernandez-Moure, Anna Mydlowska, Crystal Shin, Michael Vella, Lewis J Kaplan
Prosthetic contamination and biofilm formation continue to plague implanted materials. With increasing resistance to traditional antibiotic regimens, alternative approaches to preventing bacterial adhesion and biofilm formation have focused on the physiochemical properties of the prosthetics. Roughness, topography, hydrophobicity, porosity, charge, stiffness, and surface area all influence the processes of adhesion and colonization leading to biofilm formation. In this review, we discuss the physiochemical properties of solid and porous prosthetic materials that influence biofilm formation at the nanometric scale...
January 31, 2019: Surgical Infections
Shen Liu, Fei Wu, Shanshan Gu, Tianyi Wu, Shun Chen, Shuai Chen, Chongyang Wang, Guanlan Huang, Tuo Jin, Wenguo Cui, Bruno Sarmento, Lianfu Deng, Cunyi Fan
Sustained delivery of small interfering RNA (siRNA) is a challenge in gene silencing for managing gene-related disorders. Although nanoparticle-mediated electrospun fibers enable sustainable gene silencing, low efficiency, loss of biological activity, toxicity issues, and complex electrospinning techniques are all bottlenecks of these systems. Preventing peritendinous adhesion is crucial for their successful use, which involves blocking cellular signaling via physical barriers. Here, a multifunctional, yet structurally simple, cationic 2,6-pyridinedicarboxaldehyde-polyethylenimine (PDA)-mediated extracellular signal-regulated kinase (ERK)2-siRNA polymeric delivery system is reported, in the form of peritendinous antiadhesion electrospun poly-l-lactic acid/hyaluronan membranes (P/H), with the ability to perform sustained release of bioactive siRNA for long-term prevention of adhesions and ERK2 silencing...
January 23, 2019: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Zhixiao Yao, Wei Zheng, Xiangqi Zhang, Hao Xiong, Yun Qian, Cunyi Fan
Peritendinous fibrosis, which leads to impaired tendon function, is a clinical problem worldwide, and it is urgent to explore potential ways to reduce the formation of peritendinous adhesion. Several studies have demonstrated the biological roles of hydroxycamptothecin (HCPT) in inhibiting fibrosis in different tissues. In this study, we investigated whether HCPT could inhibit tendon fibrosis in vitro. Our results revealed that HCPT inhibited transforming growth factor (TGF)-β1-induced cell viability of human fibroblasts, decreased excessive cell hyperproliferation and promoted fibroblasts apoptosis...
January 28, 2019: IUBMB Life
Gaoxiang Zhao, Liyan Gong, Dan Su, Yujuan Jin, Chenchen Guo, Meiting Yue, Shun Yao, Zhen Qin, Yi Ye, Ying Tang, Qibiao Wu, Jian Zhang, Binghai Cui, Qiurong Ding, Hsinyi Huang, Liang Hu, Yuting Chen, Peiyuan Zhang, Guohong Hu, Luonan Chen, Kwok-Kin Wong, Daming Gao, Hongbin Ji
Metastasis is the dominant cause of patient death in small-cell lung cancer (SCLC), and a better understanding of the molecular mechanisms underlying SCLC metastasis may potentially improve clinical treatment. Through genome-scale screening for key regulators of mouse Rb1-/- Trp53-/- SCLC metastasis using the pooled CRISPR/Cas9 library, we identified Cullin5 (CUL5) and suppressor of cytokine signaling 3 (SOCS3), two components of the Cullin-RING E3 ubiquitin ligase complex, as top candidates. Mechanistically, the deficiency of CUL5 or SOCS3 disrupted the functional formation of the E3 ligase complex and prevented the degradation of integrin β1, which stabilized integrin β1 and activated downstream focal adhesion kinase/SRC (FAK/SRC) signaling and eventually drove SCLC metastasis...
January 28, 2019: Journal of Clinical Investigation
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