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Recovery from stem cell treatment

Hai Nie, Eva Kubrova, Tao Wu, Janet M Denbeigh, Christine Hunt, Allan B Dietz, Jay Smith, Wenchun Qu, Andre J van Wijnen
OBJECTIVE: To assess the biological effects of lidocaine on the viability, proliferation and function of human adipose-tissue derived mesenchymal stromal/stem cells (MSCs) in vitro. METHODS: Adipose-derived MSCs from three donors were exposed to lidocaine at various dilutions (2mg/ml-8mg/ml) and exposure times (0.5 to 4 hours). Cell number and viability, mitochondrial activity, and quantitative real time reverse-transcriptase polymerase chain reaction (RT-qPCR) were analyzed at 0 (immediate effects) or 24 and 48 hours (recovery effects) after treatment with lidocaine...
February 19, 2019: PM & R: the Journal of Injury, Function, and Rehabilitation
Yongbin Ma, Liyang Dong, Dan Zhou, Li Li, Wenzhe Zhang, Yu Zhen, Ting Wang, Jianhua Su, Deyu Chen, Chaoming Mao, Xuefeng Wang
Peripheral nerve injury results in limited nerve regeneration and severe functional impairment. Mesenchymal stem cells (MSCs) are a remarkable tool for peripheral nerve regeneration. The involvement of human umbilical cord MSC-derived extracellular vesicles (hUCMSC-EVs) in peripheral nerve regeneration, however, remains unknown. In this study, we evaluated functional recovery and nerve regeneration in rats that received hUCMSC-EV treatment after nerve transection. We observed that hUCMSC-EV treatment promoted the recovery of motor function and the regeneration of axons; increased the sciatic functional index; resulted in the generation of numerous axons and of several Schwann cells that surrounded individual axons; and attenuated the atrophy of the gastrocnemius muscle...
February 17, 2019: Journal of Cellular and Molecular Medicine
Elisa García, Roxana Rodríguez-Barrera, Vinnitsa Buzoianu-Anguiano, Adrian Flores-Romero, Emanuel Malagón-Axotla, Marco Guerrero-Godinez, Estefanía De la Cruz-Castillo, Laura Castillo-Carvajal, Monserrat Rivas-Gonzalez, Paola Santiago-Tovar, Ivis Morales, Cesar Borlongan, Antonio Ibarra
Spinal cord injury is a very common pathological event that has devastating functional consequences in patients. In recent years, several research groups are trying to find an effective therapy that could be applied in clinical practice. In this study, we analyzed the combination of different strategies as a potential therapy for spinal cord injury. Immunization with neural derived peptides (INDP), inhibition of glial scar formation (dipyridyl: DPY), as well as the use of biocompatible matrix (fibrin glue: FG) impregnated with bone marrow mesenchymal stem cells (MSCs) were combined and then its beneficial effects were evaluated in the induction of neuroprotection and neuroregeneration after acute SCI...
June 2019: Neural Regeneration Research
Juliana A Passipieri, Jack Dienes, Joseph Frank, Joshua Glazier, Andrew J Portell, Kausnik P Venkatesh, Jacqueline Margaret Bliley, Damian Grybowsky, Benjamin Schilling, Kacey Marra, George Joseph Christ
Severe peripheral nerve injuries have devastating consequences on quality of life in affected patients, and represent a significant unmet medical need. Destruction of nerve fibers results in denervation of targeted muscles, which subsequently undergo progressive atrophy and loss of function. Timely restoration of neural innervation to muscle fibers is crucial to preservation of muscle homeostasis and function. The goal of this study was to evaluate the impact of addition of adipose stem cells (ASCs) to polycaprolactone (PCL) nerve conduit guides on peripheral nerve repair and functional muscle recovery in the setting of a critical size nerve defect...
February 14, 2019: Tissue Engineering. Part A
Nils Waldhüter, Wolfgang Köhler, Philipp G Hemmati, Christian Jehn, Rudolf Peceny, Giang L Vuong, Renate Arnold, Jörn-Sven Kühl
The adult cerebral form of X-linked adrenoleukodystrophy (ACALD), an acute inflammatory demyelinating disease, results in a rapidly progressive neurodegeneration, typically leading to severe disability or death within a few years after onset. We have treated 15 men who had developed ACALD with allogeneic hematopoietic stem cell transplantation (HSCT) from matched donors after myeloablative conditioning with busulfan and cyclophosphamide. All patients engrafted and 11 survived (estimated survival 73 ± 11%), 8 with stable cognition and 7 of them with stable motor function (estimated event-free survival 36 ± 17%)...
February 11, 2019: Journal of Inherited Metabolic Disease
Yiyue Xu, Chun So, Hon-Ming Lam, Ming-Chiu Fung, Suk-Ying Tsang
Cancer recurrence has long been studied by oncologists while the underlying mechanisms remain unclear. Recently, we and others found that a phenomenon named apoptosis reversal leads to increased tumorigenicity in various cell models under different stimuli. Previous studies have been focused on tracking this process in vitro and in vivo; however, the isolation of real reversed cells has yet to be achieved, which limits our understanding on the consequences of apoptosis reversal. Here, we take advantage of a Caspase-3/7 Green Detection dye to label cells with activated caspases after apoptotic induction...
January 26, 2019: Journal of Visualized Experiments: JoVE
Bangrong Cai, Kyung-Joo Seong, Sun-Woong Bae, Min Suk Kook, Changju Chun, Jin Ho Lee, Won-Seok Choi, Ji-Yeon Jung, Won-Jae Kim
Microglia-mediated neuroinflammatory responses are well known to inhibit neurogenesis in the dentate gyrus (DG) of the adult hippocampus, and growing evidence indicates that therapeutic intervention to suppress microglial activation could be an effective strategy for restoring the impaired neurogenesis and memory performance. In the present study, we investigated the effects of water-soluble arginyl-diosgenin analog (Arg-DG) on the adult hippocampal neurogenesis using a central LPS-induced inflammatory mice model, along with the fundamental mechanisms in vivo and in vitro using LPS-stimulated microglial BV2 cells...
February 11, 2019: Molecular Neurobiology
Mu-Chen Yang, Ding-Han Wang, Juin-Hong Cherng, Wan-Chun Li, Po-Yen Lin, Wun-Eng Hsu, Ming-Lun Hsu
AIMS: To elucidate the role of Link N in regulating inflammatory molecules from human mesenchymal stem cells (hMSCs) under interleukin (IL)-1β stimulation in vitro and under Complete Freund's Adjuvant (CFA)-induced arthritis of the temporomandibular joint (TMJ) in vivo. METHODS: In vitro analysis of inflammatory cytokines and epithelial-mesenchymal transition (EMT) genes in hMSCs treated with Link N, IL-1β, and co-stimulation of IL-1β and Link N was undertaken using Luminex multiplex assays and real-time polymerase chain reaction, respectively...
January 2019: Journal of Oral & Facial Pain and Headache
Zheng Gang Zhang, Benjamin Buller, Michael Chopp
Stroke is a leading cause of disability worldwide, and brain injuries devastate patients and their families, but currently no drugs on the market promote neurological recovery. Limited spontaneous recovery of function as a result of brain remodelling after stroke or injury does occur, and cell-based therapies have been used to promote these endogenous processes. Increasing evidence is demonstrating that the positive effects of such cell-based therapy are mediated by exosomes released from the administered cells and that the microRNA cargo in these exosomes is largely responsible for the therapeutic effects...
January 30, 2019: Nature Reviews. Neurology
Mayank Verma, Yoko Asakura, Atsushi Asakura
INTRODUCTION: The vasculature and blood flow in muscle is perturbed in Duchenne muscular dystrophy (DMD) and its mdx mouse model. MicroRNA-92a (miR-92a) is enriched in endothelial cells, especially during ischemic injury. METHODS: Since antagonizing miR-92a was shown to result in increased proliferation and migration of endothelial cells and recovery from ischemia, we assessed the effects of Antagomir-92a in vitro in muscle stem cell culture and in vivo in mdx mice...
January 30, 2019: Muscle & Nerve
Zheng-Li Xu, Ming Zhou, Jin-Song Jia, Wen-Jian Mo, Xiao-Hui Zhang, Yu-Ping Zhang, Yu Wang, Yu-Miao Li, Xiao-Jun Huang, Shun-Qing Wang, Lan-Ping Xu
Our study aimed to compare treatment outcomes between hematopoietic stem cell transplantation (HSCT) from haploidentical donors (HID) and immunosuppressive therapy (IST) in adults with acquired severe aplastic anemia (SAA). The medical records of 113 SAA adults who received IST, including rabbit ATG and cyclosporin (N = 37), or HID HSCT (N = 76) within 6 months of diagnosis at two institutions were retrospectively reviewed. Estimated 8-year overall survival (OS) was comparable between the IST and HID HSCT groups (75...
January 22, 2019: Bone Marrow Transplantation
Ahmad O Khalifa, Michael Kavran, Amr Mahran, Ilaha Isali, Juliana Woda, Chris A Flask, Marc S Penn, Adonis K Hijaz
INTRODUCTION AND HYPOTHESIS: SDF-1 chemokine enhances tissue regeneration through stem cell chemotaxis, neovascularization and neuronal regeneration. We hypothesized that non-viral delivery of human plasmids that express SDF-1 (pSDF-1) may represent a novel regenerative therapy for stress urinary incontinence (SUI). METHODS: Seventy-six female rats underwent vaginal distention (VD). They were then divided into four groups according to treatment: pSDF-1 (n = 42), sham (n = 30), PBS (n = 1) and luciferase-tagged pSDF-1 (n = 3)...
January 21, 2019: International Urogynecology Journal
Min-Ah Koo, Seung Hee Hong, Mi Hee Lee, Byeong-Ju Kwon, Gyeung Mi Seon, Min Sung Kim, Dohyun Kim, Ki Chang Nam, Jong-Chul Park
Extensive skin loss caused by burns or diabetic ulcers may lead to major disability or even death. Therefore, cell-based therapies that enhance skin regeneration are clinically needed. Previous approaches have been applied the injections of cell suspensions and the implantation of biodegradable three-dimensional scaffolds seeded cells. However, these treatments have limits due to poor localization of the injected cells and insufficient delivery of oxygen and nutrients to cells. Recently, cell sheet-based tissue engineering has been developed to transplant cell sheets, which are cell-dense tissues without scaffolds...
January 16, 2019: Acta Biomaterialia
Oliver D Yuan, Clayton Lin, Joseph Wagner, Joehleen Archard, Peter Deng, Julian Halmai, Gerhard Bauer, Kyle Fink, Brian Fury, Nicholas Perotti, Jon Walker, Kari Pollock, Michelle Apperson, Janelle Butters, Peter Belafsky, D Gregory Farwell, Maggie Kuhn, Jan A Nolta, Johnathon D Anderson
Mesenchymal stem cells (MSCs) facilitate functional recovery in numerous animal models of inflammatory and ischemic tissue related diseases with a growing body of research suggesting that exosomes mediate many of these therapeutic effects. It remains unclear, however, which types of proteins are packaged into exosomes as compared to the cells from which they are derived. Here, using comprehensive proteomic analysis, we demonstrated that human primed MSCs secrete exosomes (pMEX) that are packaged with markedly higher fractions of specific protein subclasses as compared to their cells of origin, indicating regulation of their contents...
January 14, 2019: Stem Cells and Development
Masaaki Kitada, Toru Murakami, Shohei Wakao, Gen Li, Mari Dezawa
Direct conversion is considered a promising approach to obtain tissue-specific cells for cell therapies; however, this strategy depends on exogenous gene expression that may cause undesired adverse effects such as tumorigenesis. By optimizing the Schwann cell induction system, which was originally developed for trans-differentiation of bone marrow mesenchymal stem cells into Schwann cells, we established a system to directly convert adult human skin fibroblasts into cells comparable to authentic human Schwann cells without gene introduction...
January 13, 2019: Glia
Tomomi Yokoyama, Hiromi Yagi Mendoza, Tomoko Tanaka, Hisataka Ii, Riya Takano, Ken Yaegaki, Hiroshi Ishikawa
Liver transplantation is the most effective treatment for treating liver cirrhosis. However, a limited number of donors, graft rejection, and other complications can undermine transplant success. It is considered that cell transplantation is an alternative approach of liver transplantation. We previously developed a protocol for hepatic differentiation of cluster of differentiation 117+ stem cells isolated from human exfoliated deciduous tooth pulp (SHEDs) under hydrogen sulfide exposure. These cells showed excellent hepatic function...
January 12, 2019: Human Cell
Sara Frias, Sandra Ramos, Consuelo Salas, Bertha Molina, Silvia Sánchez, Roberto Rivera-Luna
Anticancer regimens for Hodgkin lymphoma (HL) patients include highly genotoxic drugs that have been very successful in killing tumor cells and providing a 90% disease-free survival at five years. However, some of these treatments do not have a specific cell target, damaging both cancerous and normal cells. Thus, HL survivors have a high risk of developing new primary cancers, both hematologic and solid tumors, which have been related to treatment. Several studies have shown that after treatment, HL patients and survivors present persistent chromosomal instability, including nonclonal chromosomal aberrations...
January 10, 2019: Genes
Zeinab Zarei Behjani, Jafar Ai, Masoud Soleimani, Amir Atashi, Behnaz Taheri, Somayeh Ebrahimi-Barough, Vahid Siavashi, Sadegh Shirian, Amir Ali Hamidieh
Sepsis and related disorders, especially acute lung injury (ALI), are the most challenging life-threatening diseases in the hospital intensive care unit. Complex pathophysiology, unbalanced immune condition, and high rate of mortality complicate the treatment of sepsis. Recently, cell therapy has been introduced as a promising option to recover the sepsis symptoms. The aim of this study was to investigate the therapeutic potential of human unrestricted somatic stem cells (USSCs) isolated from human umbilical cord blood in the mouse model of ALI...
January 9, 2019: Journal of Cellular Physiology
Barbara De Moerloose, Ardine Reedijk, Geertruida H de Bock, Tim Lammens, Valerie de Haas, Barbara Denys, Laurence Dedeken, Marry M van den Heuvel-Eibrink, Maroeska Te Loo, Anne Uyttebroeck, An Van Damme, Jutte Van der Werff-Ten Bosch, Jozsef Zsiros, Gertjan Kaspers, Eveline de Bont
BACKGROUND: Children with acute myeloid leukemia (AML) have a 70% survival rate with treatment regimens containing high doses of cytarabine and anthracyclines and, in some, hematopoietic stem cell transplantation (allo-HSCT). PROCEDURE: In this multicenter Dutch-Belgian protocol (DB AML-01), 112 children with de novo AML were included. Treatment was stratified according to day 15 bone marrow response after the first induction course. Poor responders received a second course without delay while good responders awaited hematological recovery...
January 8, 2019: Pediatric Blood & Cancer
Kaiming Liu, Liang Guo, Zhijian Zhou, Mengxiong Pan, Chuanzhu Yan
Mesenchymal stem cells can be used as a novel treatment of ischemic stroke, but their therapeutic effect and mechanism of action require further evaluation. Mitochondrial dysfunction has core functions in ischemia-reperfusion stroke injury. Our recent research has demonstrated that mesenchymal stem cells can transfer their functional mitochondria to injured endothelial cells via tunneling nanotubes in vitro, resulting in the rescue of aerobic respiration and protection of endothelial cells from apoptosis. Therefore, we presume that the mechanisms of mitochondrial protection may be involved in stem cell-mediated rescue of injured cerebral microvasculature and recovery from ischemic stroke...
January 3, 2019: Microvascular Research
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